Putative flagellar radial spoke protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Flagellar radial spoke protein 4/6

Species:

Leishmania braziliensis

UniProt:

A4H732_LEIBR

TriTrypDb:

LbrM.13.0250 , LBRM2903_130007900

Length:

592

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0001534	radial spoke	2	12
GO:0005929	cilium	4	12
GO:0031514	motile cilium	5	12
GO:0032991	protein-containing complex	1	12
GO:0042995	cell projection	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0110165	cellular anatomical entity	1	12
GO:0120025	plasma membrane bounded cell projection	3	12
GO:0005930	axoneme	2	1
GO:0097730	non-motile cilium	5	1

Expansion

Sequence features

A4H732

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H732

Function

Biological processes
Term	Name	Level	Count
GO:0003341	cilium movement	4	12
GO:0006996	organelle organization	4	12
GO:0007017	microtubule-based process	2	12
GO:0007018	microtubule-based movement	3	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0022607	cellular component assembly	4	12
GO:0030030	cell projection organization	4	11
GO:0030031	cell projection assembly	5	11
GO:0044782	cilium organization	5	11
GO:0060271	cilium assembly	6	11
GO:0060294	cilium movement involved in cell motility	5	12
GO:0070925	organelle assembly	5	11
GO:0071840	cellular component organization or biogenesis	2	12
GO:0120031	plasma membrane bounded cell projection assembly	6	11
GO:0120036	plasma membrane bounded cell projection organization	5	11
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0001578	microtubule bundle formation	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0035082	axoneme assembly	5	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	193	195	PF00675	0.291
CLV_NRD_NRD_1	213	215	PF00675	0.119
CLV_NRD_NRD_1	327	329	PF00675	0.336
CLV_NRD_NRD_1	569	571	PF00675	0.547
CLV_NRD_NRD_1	91	93	PF00675	0.353
CLV_PCSK_FUR_1	191	195	PF00082	0.336
CLV_PCSK_KEX2_1	193	195	PF00082	0.275
CLV_PCSK_KEX2_1	263	265	PF00082	0.258
CLV_PCSK_KEX2_1	521	523	PF00082	0.295
CLV_PCSK_KEX2_1	569	571	PF00082	0.547
CLV_PCSK_KEX2_1	91	93	PF00082	0.324
CLV_PCSK_PC1ET2_1	263	265	PF00082	0.261
CLV_PCSK_PC1ET2_1	521	523	PF00082	0.305
CLV_PCSK_SKI1_1	160	164	PF00082	0.254
CLV_PCSK_SKI1_1	263	267	PF00082	0.260
CLV_PCSK_SKI1_1	331	335	PF00082	0.249
CLV_PCSK_SKI1_1	97	101	PF00082	0.311
DEG_APCC_DBOX_1	286	294	PF00400	0.442
DEG_SCF_SKP2-CKS1_1	460	467	PF00560	0.536
DOC_CYCLIN_yCln2_LP_2	270	276	PF00134	0.471
DOC_MAPK_gen_1	211	221	PF00069	0.442
DOC_MAPK_gen_1	331	340	PF00069	0.536
DOC_MAPK_gen_1	521	528	PF00069	0.461
DOC_PP2B_LxvP_1	128	131	PF13499	0.536
DOC_PP2B_LxvP_1	270	273	PF13499	0.404
DOC_PP2B_LxvP_1	338	341	PF13499	0.536
DOC_PP2B_LxvP_1	416	419	PF13499	0.497
DOC_PP2B_LxvP_1	434	437	PF13499	0.499
DOC_PP4_FxxP_1	100	103	PF00568	0.491
DOC_USP7_MATH_1	239	243	PF00917	0.555
DOC_USP7_MATH_1	419	423	PF00917	0.402
DOC_USP7_MATH_1	546	550	PF00917	0.555
DOC_USP7_UBL2_3	175	179	PF12436	0.525
DOC_USP7_UBL2_3	211	215	PF12436	0.504
DOC_USP7_UBL2_3	375	379	PF12436	0.461
DOC_WW_Pin1_4	237	242	PF00397	0.502
DOC_WW_Pin1_4	249	254	PF00397	0.395
DOC_WW_Pin1_4	339	344	PF00397	0.556
DOC_WW_Pin1_4	461	466	PF00397	0.536
DOC_WW_Pin1_4	537	542	PF00397	0.448
LIG_14-3-3_CanoR_1	232	236	PF00244	0.450
LIG_14-3-3_CanoR_1	292	299	PF00244	0.496
LIG_14-3-3_CanoR_1	522	528	PF00244	0.554
LIG_BIR_II_1	1	5	PF00653	0.577
LIG_BIR_III_3	1	5	PF00653	0.427
LIG_BIR_III_4	321	325	PF00653	0.375
LIG_BIR_III_4	398	402	PF00653	0.555
LIG_BRCT_BRCA1_1	184	188	PF00533	0.527
LIG_FHA_1	120	126	PF00498	0.445
LIG_FHA_1	232	238	PF00498	0.515
LIG_FHA_1	265	271	PF00498	0.475
LIG_FHA_1	486	492	PF00498	0.475
LIG_FHA_1	559	565	PF00498	0.469
LIG_FHA_2	107	113	PF00498	0.450
LIG_FHA_2	181	187	PF00498	0.491
LIG_FHA_2	312	318	PF00498	0.555
LIG_FHA_2	386	392	PF00498	0.375
LIG_Integrin_isoDGR_2	370	372	PF01839	0.261
LIG_KLC1_Yacidic_2	200	205	PF13176	0.536
LIG_LIR_Gen_1	115	125	PF02991	0.450
LIG_LIR_Gen_1	132	138	PF02991	0.491
LIG_LIR_Gen_1	242	253	PF02991	0.494
LIG_LIR_Gen_1	31	40	PF02991	0.460
LIG_LIR_Gen_1	357	368	PF02991	0.407
LIG_LIR_Nem_3	115	120	PF02991	0.450
LIG_LIR_Nem_3	132	136	PF02991	0.491
LIG_LIR_Nem_3	185	190	PF02991	0.460
LIG_LIR_Nem_3	224	230	PF02991	0.473
LIG_LIR_Nem_3	242	248	PF02991	0.375
LIG_LIR_Nem_3	31	36	PF02991	0.460
LIG_LIR_Nem_3	332	338	PF02991	0.461
LIG_LIR_Nem_3	357	363	PF02991	0.407
LIG_LIR_Nem_3	415	420	PF02991	0.450
LIG_LIR_Nem_3	423	429	PF02991	0.430
LIG_MLH1_MIPbox_1	184	188	PF16413	0.527
LIG_MYND_1	432	436	PF01753	0.437
LIG_NRBOX	38	44	PF00104	0.555
LIG_Pex14_1	523	527	PF04695	0.461
LIG_Pex14_2	177	181	PF04695	0.491
LIG_REV1ctd_RIR_1	174	183	PF16727	0.491
LIG_SH2_CRK	117	121	PF00017	0.461
LIG_SH2_CRK	33	37	PF00017	0.461
LIG_SH2_CRK	335	339	PF00017	0.461
LIG_SH2_NCK_1	117	121	PF00017	0.461
LIG_SH2_PTP2	227	230	PF00017	0.555
LIG_SH2_SRC	195	198	PF00017	0.507
LIG_SH2_SRC	424	427	PF00017	0.440
LIG_SH2_STAP1	233	237	PF00017	0.536
LIG_SH2_STAT3	21	24	PF00017	0.522
LIG_SH2_STAT5	117	120	PF00017	0.461
LIG_SH2_STAT5	187	190	PF00017	0.456
LIG_SH2_STAT5	195	198	PF00017	0.441
LIG_SH2_STAT5	203	206	PF00017	0.427
LIG_SH2_STAT5	21	24	PF00017	0.450
LIG_SH2_STAT5	227	230	PF00017	0.545
LIG_SH2_STAT5	233	236	PF00017	0.525
LIG_SH2_STAT5	285	288	PF00017	0.450
LIG_SH2_STAT5	366	369	PF00017	0.461
LIG_SH2_STAT5	424	427	PF00017	0.415
LIG_SH2_STAT5	527	530	PF00017	0.447
LIG_SH3_2	465	470	PF14604	0.536
LIG_SH3_3	324	330	PF00018	0.502
LIG_SH3_3	337	343	PF00018	0.553
LIG_SH3_3	374	380	PF00018	0.511
LIG_SH3_3	426	432	PF00018	0.478
LIG_SH3_3	455	461	PF00018	0.477
LIG_SH3_3	462	468	PF00018	0.453
LIG_SH3_3	540	546	PF00018	0.484
LIG_SH3_3	554	560	PF00018	0.408
LIG_SH3_4	211	218	PF00018	0.511
LIG_SUMO_SIM_par_1	336	342	PF11976	0.563
LIG_TRAF2_1	151	154	PF00917	0.461
LIG_TRAF2_1	304	307	PF00917	0.461
LIG_TRAF2_1	408	411	PF00917	0.499
LIG_TRAF2_1	449	452	PF00917	0.501
LIG_TRAF2_1	86	89	PF00917	0.536
LIG_WRC_WIRS_1	61	66	PF05994	0.536
MOD_CDK_SPxK_1	461	467	PF00069	0.536
MOD_CDK_SPxxK_3	237	244	PF00069	0.555
MOD_CK1_1	132	138	PF00069	0.511
MOD_CK1_1	295	301	PF00069	0.536
MOD_CK2_1	106	112	PF00069	0.450
MOD_CK2_1	385	391	PF00069	0.375
MOD_CK2_1	4	10	PF00069	0.586
MOD_CK2_1	559	565	PF00069	0.541
MOD_CK2_1	576	582	PF00069	0.614
MOD_Cter_Amidation	519	522	PF01082	0.261
MOD_GlcNHglycan	16	19	PF01048	0.431
MOD_GlcNHglycan	2	5	PF01048	0.647
MOD_GlcNHglycan	269	273	PF01048	0.301
MOD_GlcNHglycan	294	297	PF01048	0.189
MOD_GlcNHglycan	30	33	PF01048	0.306
MOD_GlcNHglycan	6	9	PF01048	0.557
MOD_GlcNHglycan	76	79	PF01048	0.324
MOD_GSK3_1	115	122	PF00069	0.448
MOD_GSK3_1	231	238	PF00069	0.455
MOD_GSK3_1	264	271	PF00069	0.510
MOD_GSK3_1	291	298	PF00069	0.453
MOD_GSK3_1	385	392	PF00069	0.554
MOD_GSK3_1	60	67	PF00069	0.515
MOD_N-GLC_1	493	498	PF02516	0.262
MOD_NEK2_1	158	163	PF00069	0.450
MOD_NEK2_1	235	240	PF00069	0.466
MOD_NEK2_1	64	69	PF00069	0.498
MOD_NEK2_2	485	490	PF00069	0.475
MOD_PIKK_1	80	86	PF00454	0.555
MOD_PKA_1	521	527	PF00069	0.555
MOD_PKA_2	231	237	PF00069	0.450
MOD_PKA_2	259	265	PF00069	0.555
MOD_PKA_2	291	297	PF00069	0.517
MOD_PKA_2	371	377	PF00069	0.450
MOD_PKA_2	521	527	PF00069	0.555
MOD_Plk_1	158	164	PF00069	0.450
MOD_Plk_1	80	86	PF00069	0.517
MOD_Plk_2-3	106	112	PF00069	0.525
MOD_Plk_2-3	311	317	PF00069	0.555
MOD_Plk_2-3	576	582	PF00069	0.614
MOD_Plk_4	106	112	PF00069	0.494
MOD_Plk_4	121	127	PF00069	0.524
MOD_Plk_4	231	237	PF00069	0.450
MOD_Plk_4	60	66	PF00069	0.464
MOD_ProDKin_1	237	243	PF00069	0.502
MOD_ProDKin_1	249	255	PF00069	0.395
MOD_ProDKin_1	339	345	PF00069	0.556
MOD_ProDKin_1	461	467	PF00069	0.536
MOD_ProDKin_1	537	543	PF00069	0.448
MOD_SUMO_rev_2	168	177	PF00179	0.536
MOD_SUMO_rev_2	448	458	PF00179	0.529
TRG_DiLeu_BaEn_1	153	158	PF01217	0.461
TRG_DiLeu_BaEn_2	59	65	PF01217	0.536
TRG_DiLeu_BaEn_4	153	159	PF01217	0.461
TRG_ENDOCYTIC_2	117	120	PF00928	0.450
TRG_ENDOCYTIC_2	187	190	PF00928	0.443
TRG_ENDOCYTIC_2	227	230	PF00928	0.555
TRG_ENDOCYTIC_2	33	36	PF00928	0.450
TRG_ENDOCYTIC_2	335	338	PF00928	0.461
TRG_ENDOCYTIC_2	426	429	PF00928	0.440
TRG_ER_diArg_1	190	193	PF00400	0.497
TRG_ER_diArg_1	568	570	PF00400	0.553
TRG_ER_diArg_1	90	92	PF00400	0.555
TRG_NES_CRM1_1	34	46	PF08389	0.555
TRG_NLS_MonoExtN_4	211	218	PF00514	0.536

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDF4	Leptomonas seymouri	73%	99%
A0A0S4JGY1	Bodo saltans	50%	100%
A0A1X0NN98	Trypanosomatidae	58%	99%
A0A3Q8IE67	Leishmania donovani	24%	83%
A0A3S7WSD6	Leishmania donovani	87%	98%
A0A422NNZ6	Trypanosoma rangeli	57%	99%
A4HH73	Leishmania braziliensis	24%	100%
A4HVG6	Leishmania infantum	88%	99%
A4I4B9	Leishmania infantum	24%	84%
D0A6U3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9AP67	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	99%
Q01656	Chlamydomonas reinhardtii	26%	100%
Q4QGB1	Leishmania major	88%	100%
V5BDZ5	Trypanosoma cruzi	59%	92%