Cysteine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteinyl-tRNA synthetase, putative

Species:

Leishmania braziliensis

UniProt:

A4H6U0_LEIBR

TriTrypDb:

LbrM.12.0270 , LBRM2903_120007700

Length:

780

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 2
Pissara et al.	yes	yes: 8

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 4

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 4

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H6U0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6U0

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006399	tRNA metabolic process	7	11
GO:0006418	tRNA aminoacylation for protein translation	6	11
GO:0006423	cysteinyl-tRNA aminoacylation	7	11
GO:0006520	amino acid metabolic process	3	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0019752	carboxylic acid metabolic process	5	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034660	ncRNA metabolic process	6	11
GO:0043038	amino acid activation	4	11
GO:0043039	tRNA aminoacylation	5	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043436	oxoacid metabolic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044281	small molecule metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004812	aminoacyl-tRNA ligase activity	4	11
GO:0004817	cysteine-tRNA ligase activity	5	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016874	ligase activity	2	11
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140101	catalytic activity, acting on a tRNA	4	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	609	613	PF00656	0.494
CLV_NRD_NRD_1	154	156	PF00675	0.306
CLV_NRD_NRD_1	236	238	PF00675	0.301
CLV_NRD_NRD_1	559	561	PF00675	0.395
CLV_NRD_NRD_1	660	662	PF00675	0.414
CLV_NRD_NRD_1	667	669	PF00675	0.433
CLV_NRD_NRD_1	674	676	PF00675	0.456
CLV_NRD_NRD_1	687	689	PF00675	0.289
CLV_NRD_NRD_1	705	707	PF00675	0.483
CLV_NRD_NRD_1	87	89	PF00675	0.247
CLV_PCSK_KEX2_1	113	115	PF00082	0.247
CLV_PCSK_KEX2_1	154	156	PF00082	0.306
CLV_PCSK_KEX2_1	20	22	PF00082	0.439
CLV_PCSK_KEX2_1	333	335	PF00082	0.260
CLV_PCSK_KEX2_1	667	669	PF00082	0.561
CLV_PCSK_KEX2_1	686	688	PF00082	0.293
CLV_PCSK_KEX2_1	705	707	PF00082	0.465
CLV_PCSK_KEX2_1	87	89	PF00082	0.247
CLV_PCSK_PC1ET2_1	113	115	PF00082	0.247
CLV_PCSK_PC1ET2_1	154	156	PF00082	0.342
CLV_PCSK_PC1ET2_1	20	22	PF00082	0.463
CLV_PCSK_PC1ET2_1	333	335	PF00082	0.260
CLV_PCSK_PC1ET2_1	686	688	PF00082	0.420
CLV_PCSK_SKI1_1	110	114	PF00082	0.247
CLV_PCSK_SKI1_1	155	159	PF00082	0.375
CLV_PCSK_SKI1_1	241	245	PF00082	0.244
CLV_PCSK_SKI1_1	422	426	PF00082	0.247
CLV_PCSK_SKI1_1	428	432	PF00082	0.247
CLV_PCSK_SKI1_1	437	441	PF00082	0.247
CLV_PCSK_SKI1_1	451	455	PF00082	0.247
CLV_PCSK_SKI1_1	481	485	PF00082	0.328
CLV_PCSK_SKI1_1	507	511	PF00082	0.436
CLV_PCSK_SKI1_1	677	681	PF00082	0.538
CLV_PCSK_SKI1_1	734	738	PF00082	0.485
CLV_PCSK_SKI1_1	745	749	PF00082	0.401
DEG_APCC_DBOX_1	436	444	PF00400	0.460
DOC_CYCLIN_RxL_1	448	457	PF00134	0.420
DOC_MAPK_gen_1	428	436	PF00069	0.447
DOC_PP1_RVXF_1	134	141	PF00149	0.404
DOC_PP1_RVXF_1	449	455	PF00149	0.447
DOC_PP1_RVXF_1	55	62	PF00149	0.444
DOC_PP4_FxxP_1	344	347	PF00568	0.447
DOC_PP4_FxxP_1	696	699	PF00568	0.425
DOC_USP7_MATH_1	147	151	PF00917	0.491
DOC_USP7_MATH_1	208	212	PF00917	0.509
DOC_USP7_MATH_1	651	655	PF00917	0.325
DOC_USP7_UBL2_3	154	158	PF12436	0.459
DOC_USP7_UBL2_3	734	738	PF12436	0.451
DOC_USP7_UBL2_3	741	745	PF12436	0.413
DOC_WW_Pin1_4	351	356	PF00397	0.455
DOC_WW_Pin1_4	443	448	PF00397	0.388
DOC_WW_Pin1_4	454	459	PF00397	0.388
LIG_14-3-3_CanoR_1	260	268	PF00244	0.465
LIG_14-3-3_CanoR_1	311	318	PF00244	0.503
LIG_14-3-3_CanoR_1	553	559	PF00244	0.363
LIG_14-3-3_CanoR_1	60	69	PF00244	0.475
LIG_14-3-3_CanoR_1	78	84	PF00244	0.386
LIG_ActinCP_TwfCPI_2	344	351	PF01115	0.478
LIG_BRCT_BRCA1_1	286	290	PF00533	0.458
LIG_BRCT_BRCA1_1	335	339	PF00533	0.392
LIG_BRCT_BRCA1_1	700	704	PF00533	0.447
LIG_deltaCOP1_diTrp_1	349	356	PF00928	0.447
LIG_deltaCOP1_diTrp_1	691	696	PF00928	0.520
LIG_EVH1_2	699	703	PF00568	0.311
LIG_FHA_1	260	266	PF00498	0.447
LIG_FHA_1	324	330	PF00498	0.483
LIG_FHA_1	4	10	PF00498	0.534
LIG_FHA_1	43	49	PF00498	0.378
LIG_FHA_1	447	453	PF00498	0.481
LIG_FHA_1	497	503	PF00498	0.404
LIG_FHA_1	592	598	PF00498	0.544
LIG_FHA_1	80	86	PF00498	0.447
LIG_FHA_2	103	109	PF00498	0.447
LIG_FHA_2	311	317	PF00498	0.449
LIG_FHA_2	45	51	PF00498	0.518
LIG_FHA_2	65	71	PF00498	0.375
LIG_FHA_2	711	717	PF00498	0.486
LIG_FHA_2	725	731	PF00498	0.423
LIG_LIR_Apic_2	349	355	PF02991	0.447
LIG_LIR_Apic_2	457	462	PF02991	0.460
LIG_LIR_Gen_1	201	210	PF02991	0.463
LIG_LIR_Gen_1	277	288	PF02991	0.558
LIG_LIR_Gen_1	526	534	PF02991	0.425
LIG_LIR_Gen_1	593	604	PF02991	0.552
LIG_LIR_Gen_1	713	722	PF02991	0.447
LIG_LIR_Gen_1	749	754	PF02991	0.466
LIG_LIR_Gen_1	82	90	PF02991	0.447
LIG_LIR_Nem_3	201	206	PF02991	0.449
LIG_LIR_Nem_3	277	283	PF02991	0.558
LIG_LIR_Nem_3	301	305	PF02991	0.472
LIG_LIR_Nem_3	526	531	PF02991	0.428
LIG_LIR_Nem_3	567	573	PF02991	0.373
LIG_LIR_Nem_3	593	599	PF02991	0.573
LIG_LIR_Nem_3	691	696	PF02991	0.414
LIG_LIR_Nem_3	709	715	PF02991	0.472
LIG_LIR_Nem_3	749	753	PF02991	0.463
LIG_LIR_Nem_3	82	86	PF02991	0.447
LIG_LIR_Nem_3	91	96	PF02991	0.447
LIG_LYPXL_yS_3	28	31	PF13949	0.442
LIG_LYPXL_yS_3	302	305	PF13949	0.460
LIG_MLH1_MIPbox_1	286	290	PF16413	0.447
LIG_MLH1_MIPbox_1	700	704	PF16413	0.447
LIG_MYND_1	303	307	PF01753	0.460
LIG_PCNA_PIPBox_1	617	626	PF02747	0.337
LIG_PCNA_TLS_4	87	94	PF02747	0.447
LIG_PCNA_yPIPBox_3	611	624	PF02747	0.334
LIG_Pex14_1	386	390	PF04695	0.460
LIG_Pex14_1	409	413	PF04695	0.447
LIG_Pex14_2	203	207	PF04695	0.447
LIG_REV1ctd_RIR_1	701	710	PF16727	0.355
LIG_SH2_CRK	96	100	PF00017	0.460
LIG_SH2_NCK_1	239	243	PF00017	0.542
LIG_SH2_NCK_1	715	719	PF00017	0.511
LIG_SH2_SRC	715	718	PF00017	0.511
LIG_SH2_STAP1	239	243	PF00017	0.460
LIG_SH2_STAP1	505	509	PF00017	0.276
LIG_SH2_STAP1	746	750	PF00017	0.463
LIG_SH2_STAP1	96	100	PF00017	0.503
LIG_SH2_STAT3	264	267	PF00017	0.478
LIG_SH2_STAT5	264	267	PF00017	0.451
LIG_SH2_STAT5	282	285	PF00017	0.575
LIG_SH2_STAT5	295	298	PF00017	0.508
LIG_SH2_STAT5	412	415	PF00017	0.447
LIG_SH2_STAT5	547	550	PF00017	0.364
LIG_SH2_STAT5	565	568	PF00017	0.311
LIG_SH2_STAT5	62	65	PF00017	0.459
LIG_SH2_STAT5	712	715	PF00017	0.471
LIG_SH2_STAT5	80	83	PF00017	0.400
LIG_SH2_STAT5	93	96	PF00017	0.447
LIG_SH3_3	23	29	PF00018	0.449
LIG_SH3_3	344	350	PF00018	0.447
LIG_SH3_3	643	649	PF00018	0.492
LIG_SH3_3	694	700	PF00018	0.355
LIG_SUMO_SIM_anti_2	486	491	PF11976	0.373
LIG_TRAF2_1	313	316	PF00917	0.457
LIG_TRAF2_1	669	672	PF00917	0.513
LIG_TRAF2_1	727	730	PF00917	0.549
LIG_TYR_ITIM	26	31	PF00017	0.285
LIG_TYR_ITSM	711	718	PF00017	0.410
LIG_UBA3_1	179	185	PF00899	0.221
LIG_WRC_WIRS_1	80	85	PF05994	0.282
MOD_CDK_SPK_2	443	448	PF00069	0.199
MOD_CDK_SPxxK_3	454	461	PF00069	0.204
MOD_CK1_1	259	265	PF00069	0.261
MOD_CK1_1	466	472	PF00069	0.300
MOD_CK2_1	129	135	PF00069	0.222
MOD_CK2_1	310	316	PF00069	0.331
MOD_CK2_1	40	46	PF00069	0.345
MOD_CK2_1	466	472	PF00069	0.282
MOD_CK2_1	724	730	PF00069	0.545
MOD_CK2_1	746	752	PF00069	0.401
MOD_GlcNHglycan	210	213	PF01048	0.387
MOD_GlcNHglycan	382	385	PF01048	0.323
MOD_GlcNHglycan	549	552	PF01048	0.431
MOD_GlcNHglycan	554	557	PF01048	0.339
MOD_GlcNHglycan	588	591	PF01048	0.598
MOD_GSK3_1	365	372	PF00069	0.315
MOD_GSK3_1	40	47	PF00069	0.392
MOD_GSK3_1	60	67	PF00069	0.161
MOD_GSK3_1	647	654	PF00069	0.557
MOD_GSK3_1	716	723	PF00069	0.462
MOD_N-GLC_1	102	107	PF02516	0.282
MOD_N-GLC_1	3	8	PF02516	0.419
MOD_N-GLC_1	311	316	PF02516	0.282
MOD_N-GLC_1	463	468	PF02516	0.300
MOD_N-GLC_2	395	397	PF02516	0.282
MOD_NEK2_1	129	134	PF00069	0.204
MOD_NEK2_1	184	189	PF00069	0.309
MOD_NEK2_1	424	429	PF00069	0.282
MOD_NEK2_1	94	99	PF00069	0.415
MOD_NEK2_2	284	289	PF00069	0.330
MOD_PIKK_1	184	190	PF00454	0.325
MOD_PIKK_1	496	502	PF00454	0.458
MOD_PKA_1	333	339	PF00069	0.204
MOD_PKA_1	677	683	PF00069	0.412
MOD_PKA_1	710	716	PF00069	0.405
MOD_PKA_1	737	743	PF00069	0.460
MOD_PKA_2	259	265	PF00069	0.282
MOD_PKA_2	310	316	PF00069	0.415
MOD_PKA_2	333	339	PF00069	0.208
MOD_PKA_2	552	558	PF00069	0.357
MOD_Plk_1	284	290	PF00069	0.325
MOD_Plk_1	365	371	PF00069	0.282
MOD_Plk_1	466	472	PF00069	0.282
MOD_Plk_1	591	597	PF00069	0.345
MOD_Plk_1	69	75	PF00069	0.300
MOD_Plk_2-3	729	735	PF00069	0.524
MOD_Plk_4	284	290	PF00069	0.330
MOD_Plk_4	365	371	PF00069	0.282
MOD_Plk_4	527	533	PF00069	0.413
MOD_Plk_4	536	542	PF00069	0.350
MOD_Plk_4	554	560	PF00069	0.244
MOD_Plk_4	64	70	PF00069	0.282
MOD_Plk_4	757	763	PF00069	0.568
MOD_Plk_4	768	774	PF00069	0.474
MOD_Plk_4	94	100	PF00069	0.346
MOD_ProDKin_1	351	357	PF00069	0.293
MOD_ProDKin_1	443	449	PF00069	0.199
MOD_ProDKin_1	454	460	PF00069	0.199
MOD_SUMO_rev_2	106	112	PF00179	0.282
MOD_SUMO_rev_2	174	180	PF00179	0.283
MOD_SUMO_rev_2	383	391	PF00179	0.300
MOD_SUMO_rev_2	612	620	PF00179	0.449
MOD_SUMO_rev_2	708	713	PF00179	0.530
MOD_SUMO_rev_2	729	739	PF00179	0.469
TRG_DiLeu_BaLyEn_6	448	453	PF01217	0.282
TRG_ENDOCYTIC_2	28	31	PF00928	0.442
TRG_ENDOCYTIC_2	302	305	PF00928	0.306
TRG_ENDOCYTIC_2	460	463	PF00928	0.204
TRG_ENDOCYTIC_2	715	718	PF00928	0.445
TRG_ENDOCYTIC_2	750	753	PF00928	0.430
TRG_ENDOCYTIC_2	80	83	PF00928	0.282
TRG_ENDOCYTIC_2	96	99	PF00928	0.282
TRG_ER_diArg_1	114	117	PF00400	0.282
TRG_ER_diArg_1	687	689	PF00400	0.538
TRG_ER_diArg_1	704	706	PF00400	0.481
TRG_ER_diArg_1	86	88	PF00400	0.282
TRG_NLS_Bipartite_1	661	680	PF00514	0.405
TRG_NLS_MonoExtC_3	153	158	PF00514	0.295
TRG_NLS_MonoExtC_3	685	691	PF00514	0.418
TRG_NLS_MonoExtN_4	110	117	PF00514	0.282
TRG_NLS_MonoExtN_4	154	159	PF00514	0.295
TRG_NLS_MonoExtN_4	675	680	PF00514	0.457
TRG_Pf-PMV_PEXEL_1	662	666	PF00026	0.504
TRG_Pf-PMV_PEXEL_1	687	691	PF00026	0.419
TRG_Pf-PMV_PEXEL_1	87	91	PF00026	0.250

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2U0	Leptomonas seymouri	81%	99%
A0A0S4JIJ5	Bodo saltans	64%	100%
A0A1X0NM71	Trypanosomatidae	69%	98%
A0A3S7WRW6	Leishmania donovani	89%	99%
A0A422N5J6	Trypanosoma rangeli	68%	99%
C9ZQB2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	67%	99%
E9AGB4	Leishmania infantum	89%	99%
E9ANV2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	99%
P49589	Homo sapiens	40%	100%
P53852	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	39%	100%
Q09860	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	100%
Q291L4	Drosophila pseudoobscura pseudoobscura	41%	100%
Q4QGR8	Leishmania major	89%	100%
Q4R550	Macaca fascicularis	41%	100%
Q54KR1	Dictyostelium discoideum	45%	100%
Q5F408	Gallus gallus	40%	100%
Q5M7N8	Xenopus tropicalis	41%	100%
Q7KN90	Drosophila melanogaster	41%	100%
Q7ZWR2	Xenopus laevis	41%	100%
Q9ER72	Mus musculus	39%	94%