tRNA pseudouridine synthase D (TruD)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase D (TruD), putative

Species:

Leishmania braziliensis

UniProt:

A4H6R0_LEIBR

TriTrypDb:

LbrM.11.1140 , LBRM2903_110019700

Length:

618

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H6R0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6R0

PDB structure(s): 7am2_CA

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	13
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0009451	RNA modification	5	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0043170	macromolecule metabolic process	3	13
GO:0043412	macromolecule modification	4	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	13
GO:0003723	RNA binding	4	13
GO:0003824	catalytic activity	1	13
GO:0005488	binding	1	13
GO:0009982	pseudouridine synthase activity	4	13
GO:0016853	isomerase activity	2	13
GO:0016866	intramolecular transferase activity	3	13
GO:0097159	organic cyclic compound binding	2	13
GO:1901363	heterocyclic compound binding	2	13
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	439	443	PF00656	0.574
CLV_C14_Caspase3-7	458	462	PF00656	0.365
CLV_C14_Caspase3-7	87	91	PF00656	0.432
CLV_NRD_NRD_1	106	108	PF00675	0.471
CLV_NRD_NRD_1	143	145	PF00675	0.520
CLV_NRD_NRD_1	264	266	PF00675	0.266
CLV_NRD_NRD_1	360	362	PF00675	0.527
CLV_NRD_NRD_1	391	393	PF00675	0.471
CLV_NRD_NRD_1	425	427	PF00675	0.578
CLV_NRD_NRD_1	44	46	PF00675	0.527
CLV_NRD_NRD_1	553	555	PF00675	0.474
CLV_NRD_NRD_1	577	579	PF00675	0.565
CLV_PCSK_KEX2_1	143	145	PF00082	0.569
CLV_PCSK_KEX2_1	264	266	PF00082	0.367
CLV_PCSK_KEX2_1	360	362	PF00082	0.287
CLV_PCSK_KEX2_1	391	393	PF00082	0.471
CLV_PCSK_KEX2_1	424	426	PF00082	0.565
CLV_PCSK_KEX2_1	44	46	PF00082	0.527
CLV_PCSK_KEX2_1	552	554	PF00082	0.510
CLV_PCSK_KEX2_1	577	579	PF00082	0.606
CLV_PCSK_PC1ET2_1	552	554	PF00082	0.596
CLV_PCSK_PC7_1	356	362	PF00082	0.278
CLV_PCSK_PC7_1	573	579	PF00082	0.312
CLV_PCSK_SKI1_1	113	117	PF00082	0.482
CLV_PCSK_SKI1_1	152	156	PF00082	0.475
CLV_PCSK_SKI1_1	249	253	PF00082	0.350
CLV_PCSK_SKI1_1	356	360	PF00082	0.530
CLV_PCSK_SKI1_1	409	413	PF00082	0.558
CLV_PCSK_SKI1_1	416	420	PF00082	0.541
CLV_PCSK_SKI1_1	601	605	PF00082	0.386
CLV_PCSK_SKI1_1	94	98	PF00082	0.512
CLV_Separin_Metazoa	235	239	PF03568	0.539
CLV_Separin_Metazoa	357	361	PF03568	0.532
DEG_APCC_DBOX_1	248	256	PF00400	0.346
DEG_APCC_DBOX_1	264	272	PF00400	0.365
DEG_APCC_DBOX_1	355	363	PF00400	0.532
DEG_Nend_UBRbox_1	1	4	PF02207	0.478
DEG_ODPH_VHL_1	241	252	PF01847	0.254
DEG_SPOP_SBC_1	494	498	PF00917	0.490
DOC_CYCLIN_RxL_1	107	120	PF00134	0.503
DOC_CYCLIN_RxL_1	261	270	PF00134	0.511
DOC_MAPK_DCC_7	216	225	PF00069	0.306
DOC_MAPK_gen_1	107	117	PF00069	0.469
DOC_MAPK_gen_1	170	179	PF00069	0.465
DOC_MAPK_gen_1	264	271	PF00069	0.531
DOC_PP1_RVXF_1	407	414	PF00149	0.539
DOC_PP2B_LxvP_1	177	180	PF13499	0.493
DOC_PP2B_LxvP_1	453	456	PF13499	0.411
DOC_PP4_FxxP_1	124	127	PF00568	0.440
DOC_USP7_MATH_1	127	131	PF00917	0.479
DOC_USP7_MATH_1	18	22	PF00917	0.610
DOC_USP7_MATH_1	190	194	PF00917	0.422
DOC_USP7_MATH_1	212	216	PF00917	0.407
DOC_USP7_MATH_1	23	27	PF00917	0.465
DOC_USP7_MATH_1	302	306	PF00917	0.506
DOC_USP7_MATH_1	404	408	PF00917	0.353
DOC_USP7_MATH_1	441	445	PF00917	0.567
DOC_USP7_MATH_1	456	460	PF00917	0.505
DOC_USP7_MATH_1	468	472	PF00917	0.519
DOC_USP7_MATH_1	503	507	PF00917	0.391
DOC_USP7_MATH_1	543	547	PF00917	0.451
DOC_USP7_MATH_1	57	61	PF00917	0.609
DOC_USP7_MATH_1	591	595	PF00917	0.436
DOC_WW_Pin1_4	119	124	PF00397	0.497
DOC_WW_Pin1_4	314	319	PF00397	0.484
DOC_WW_Pin1_4	53	58	PF00397	0.619
DOC_WW_Pin1_4	556	561	PF00397	0.422
DOC_WW_Pin1_4	592	597	PF00397	0.417
LIG_14-3-3_CanoR_1	111	116	PF00244	0.492
LIG_14-3-3_CanoR_1	134	139	PF00244	0.458
LIG_14-3-3_CanoR_1	228	234	PF00244	0.501
LIG_14-3-3_CanoR_1	238	242	PF00244	0.548
LIG_14-3-3_CanoR_1	267	272	PF00244	0.458
LIG_14-3-3_CanoR_1	409	414	PF00244	0.504
LIG_14-3-3_CanoR_1	424	432	PF00244	0.547
LIG_14-3-3_CanoR_1	493	503	PF00244	0.641
LIG_14-3-3_CanoR_1	51	56	PF00244	0.563
LIG_14-3-3_CanoR_1	78	86	PF00244	0.488
LIG_Actin_WH2_2	141	158	PF00022	0.437
LIG_APCC_ABBA_1	157	162	PF00400	0.364
LIG_APCC_ABBAyCdc20_2	156	162	PF00400	0.381
LIG_BIR_III_4	90	94	PF00653	0.490
LIG_BRCT_BRCA1_1	532	536	PF00533	0.477
LIG_BRCT_BRCA1_1	563	567	PF00533	0.453
LIG_eIF4E_1	218	224	PF01652	0.429
LIG_eIF4E_1	246	252	PF01652	0.485
LIG_FHA_1	112	118	PF00498	0.453
LIG_FHA_1	467	473	PF00498	0.559
LIG_FHA_2	230	236	PF00498	0.439
LIG_FHA_2	287	293	PF00498	0.481
LIG_FHA_2	310	316	PF00498	0.500
LIG_FHA_2	33	39	PF00498	0.467
LIG_FHA_2	445	451	PF00498	0.471
LIG_FHA_2	608	614	PF00498	0.478
LIG_FHA_2	64	70	PF00498	0.419
LIG_FHA_2	85	91	PF00498	0.426
LIG_HP1_1	596	600	PF01393	0.496
LIG_Integrin_RGD_1	372	374	PF01839	0.432
LIG_LIR_Apic_2	122	127	PF02991	0.437
LIG_LIR_Gen_1	193	202	PF02991	0.490
LIG_LIR_Gen_1	227	237	PF02991	0.495
LIG_LIR_Gen_1	341	349	PF02991	0.534
LIG_LIR_Gen_1	474	481	PF02991	0.441
LIG_LIR_Gen_1	594	604	PF02991	0.394
LIG_LIR_Gen_1	610	617	PF02991	0.341
LIG_LIR_Nem_3	137	141	PF02991	0.440
LIG_LIR_Nem_3	193	197	PF02991	0.376
LIG_LIR_Nem_3	227	233	PF02991	0.476
LIG_LIR_Nem_3	270	275	PF02991	0.369
LIG_LIR_Nem_3	341	345	PF02991	0.508
LIG_LIR_Nem_3	474	480	PF02991	0.460
LIG_LIR_Nem_3	505	510	PF02991	0.416
LIG_LIR_Nem_3	594	600	PF02991	0.392
LIG_LIR_Nem_3	610	614	PF02991	0.333
LIG_NRBOX	246	252	PF00104	0.387
LIG_Pex14_1	547	551	PF04695	0.546
LIG_Pex14_2	274	278	PF04695	0.347
LIG_SH2_CRK	272	276	PF00017	0.244
LIG_SH2_GRB2like	327	330	PF00017	0.425
LIG_SH2_NCK_1	327	331	PF00017	0.534
LIG_SH2_SRC	327	330	PF00017	0.425
LIG_SH2_STAT5	141	144	PF00017	0.470
LIG_SH2_STAT5	185	188	PF00017	0.494
LIG_SH2_STAT5	246	249	PF00017	0.495
LIG_SH2_STAT5	277	280	PF00017	0.326
LIG_SH2_STAT5	309	312	PF00017	0.416
LIG_SH2_STAT5	324	327	PF00017	0.308
LIG_SH2_STAT5	446	449	PF00017	0.560
LIG_SH3_3	448	454	PF00018	0.408
LIG_SH3_3	508	514	PF00018	0.445
LIG_SH3_3	552	558	PF00018	0.441
LIG_SUMO_SIM_par_1	112	120	PF11976	0.465
LIG_TYR_ITIM	275	280	PF00017	0.336
LIG_WRC_WIRS_1	410	415	PF05994	0.555
LIG_WRC_WIRS_1	608	613	PF05994	0.447
MOD_CK1_1	165	171	PF00069	0.391
MOD_CK1_1	193	199	PF00069	0.319
MOD_CK1_1	229	235	PF00069	0.428
MOD_CK1_1	26	32	PF00069	0.530
MOD_CK1_1	393	399	PF00069	0.481
MOD_CK1_1	444	450	PF00069	0.556
MOD_CK1_1	496	502	PF00069	0.586
MOD_CK1_1	56	62	PF00069	0.491
MOD_CK1_1	84	90	PF00069	0.473
MOD_CK2_1	134	140	PF00069	0.458
MOD_CK2_1	229	235	PF00069	0.440
MOD_CK2_1	309	315	PF00069	0.411
MOD_CK2_1	468	474	PF00069	0.538
MOD_CK2_1	56	62	PF00069	0.598
MOD_CK2_1	63	69	PF00069	0.581
MOD_GlcNHglycan	20	23	PF01048	0.581
MOD_GlcNHglycan	394	398	PF01048	0.422
MOD_GlcNHglycan	428	431	PF01048	0.520
MOD_GlcNHglycan	498	501	PF01048	0.727
MOD_GSK3_1	107	114	PF00069	0.462
MOD_GSK3_1	309	316	PF00069	0.379
MOD_GSK3_1	47	54	PF00069	0.513
MOD_GSK3_1	489	496	PF00069	0.596
MOD_GSK3_1	520	527	PF00069	0.328
MOD_GSK3_1	541	548	PF00069	0.546
MOD_GSK3_1	556	563	PF00069	0.427
MOD_N-GLC_1	484	489	PF02516	0.291
MOD_N-GLC_1	561	566	PF02516	0.461
MOD_NEK2_1	12	17	PF00069	0.602
MOD_NEK2_1	313	318	PF00069	0.391
MOD_NEK2_1	489	494	PF00069	0.606
MOD_NEK2_2	23	28	PF00069	0.355
MOD_NEK2_2	286	291	PF00069	0.476
MOD_PIKK_1	195	201	PF00454	0.374
MOD_PIKK_1	365	371	PF00454	0.568
MOD_PIKK_1	404	410	PF00454	0.371
MOD_PIKK_1	81	87	PF00454	0.279
MOD_PKA_1	107	113	PF00069	0.468
MOD_PKA_1	424	430	PF00069	0.415
MOD_PKA_2	237	243	PF00069	0.534
MOD_PKA_2	390	396	PF00069	0.464
MOD_PKA_2	424	430	PF00069	0.594
MOD_PKA_2	456	462	PF00069	0.610
MOD_PKA_2	569	575	PF00069	0.535
MOD_PKB_1	105	113	PF00069	0.567
MOD_PKB_1	265	273	PF00069	0.527
MOD_PKB_1	424	432	PF00069	0.414
MOD_Plk_1	127	133	PF00069	0.556
MOD_Plk_1	226	232	PF00069	0.452
MOD_Plk_1	441	447	PF00069	0.558
MOD_Plk_1	484	490	PF00069	0.415
MOD_Plk_1	580	586	PF00069	0.516
MOD_Plk_2-3	162	168	PF00069	0.500
MOD_Plk_2-3	38	44	PF00069	0.312
MOD_Plk_2-3	436	442	PF00069	0.559
MOD_Plk_2-3	545	551	PF00069	0.546
MOD_Plk_4	111	117	PF00069	0.457
MOD_Plk_4	23	29	PF00069	0.352
MOD_Plk_4	267	273	PF00069	0.446
MOD_Plk_4	286	292	PF00069	0.462
MOD_Plk_4	484	490	PF00069	0.430
MOD_ProDKin_1	119	125	PF00069	0.499
MOD_ProDKin_1	314	320	PF00069	0.481
MOD_ProDKin_1	53	59	PF00069	0.628
MOD_ProDKin_1	556	562	PF00069	0.416
MOD_ProDKin_1	592	598	PF00069	0.414
MOD_SUMO_for_1	531	534	PF00179	0.267
TRG_DiLeu_BaEn_1	354	359	PF01217	0.413
TRG_DiLeu_BaLyEn_6	219	224	PF01217	0.339
TRG_DiLeu_BaLyEn_6	246	251	PF01217	0.378
TRG_DiLeu_BaLyEn_6	264	269	PF01217	0.418
TRG_DiLeu_BaLyEn_6	406	411	PF01217	0.371
TRG_DiLeu_LyEn_5	150	155	PF01217	0.495
TRG_ENDOCYTIC_2	230	233	PF00928	0.493
TRG_ENDOCYTIC_2	272	275	PF00928	0.358
TRG_ENDOCYTIC_2	277	280	PF00928	0.332
TRG_ENDOCYTIC_2	507	510	PF00928	0.398
TRG_ENDOCYTIC_2	608	611	PF00928	0.394
TRG_ER_diArg_1	142	144	PF00400	0.559
TRG_ER_diArg_1	169	172	PF00400	0.477
TRG_ER_diArg_1	264	267	PF00400	0.279
TRG_ER_diArg_1	359	361	PF00400	0.285
TRG_ER_diArg_1	390	392	PF00400	0.491
TRG_ER_diArg_1	423	426	PF00400	0.483
TRG_ER_diArg_1	553	556	PF00400	0.500
TRG_NLS_MonoExtC_3	551	556	PF00514	0.321
TRG_Pf-PMV_PEXEL_1	37	42	PF00026	0.324

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P414	Leptomonas seymouri	62%	98%
A0A0S4JV05	Bodo saltans	38%	94%
A0A1X0NUT8	Trypanosomatidae	44%	100%
A0A3S7WRT6	Leishmania donovani	80%	100%
A0A422N563	Trypanosoma rangeli	46%	100%
A4HV43	Leishmania infantum	80%	100%
C9ZZ88	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	100%
D0A7A5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	99%
E9ANS4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
Q4QGU5	Leishmania major	79%	100%
V5BBF7	Trypanosoma cruzi	43%	100%