Putative pretranslocation protein, alpha subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein transport protein SEC61 subunit alpha, putative

Species:

Leishmania braziliensis

UniProt:

A4H6N1_LEIBR

TriTrypDb:

LbrM.11.0850 , LBRM2903_110015200

Length:

486

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	12
GO:0110165	cellular anatomical entity	1	12
GO:0005784	Sec61 translocon complex	4	1
GO:0032991	protein-containing complex	1	1
GO:0071256	translocon complex	3	1
GO:0098796	membrane protein complex	2	1
GO:0140534	endoplasmic reticulum protein-containing complex	2	1

Expansion

Sequence features

A4H6N1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6N1

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0008104	protein localization	4	12
GO:0009987	cellular process	1	12
GO:0015031	protein transport	4	12
GO:0033036	macromolecule localization	2	12
GO:0045184	establishment of protein localization	3	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0051641	cellular localization	2	12
GO:0070727	cellular macromolecule localization	3	12
GO:0071702	organic substance transport	4	12
GO:0071705	nitrogen compound transport	4	12
GO:0006616	SRP-dependent cotranslational protein targeting to membrane, translocation	5	1
GO:0006886	intracellular protein transport	4	1
GO:0031204	post-translational protein targeting to membrane, translocation	5	1
GO:0046907	intracellular transport	3	1
GO:0051649	establishment of localization in cell	3	1
GO:0055085	transmembrane transport	2	1
GO:0065002	intracellular protein transmembrane transport	4	1
GO:0071806	protein transmembrane transport	3	1

Molecular functions
Term	Name	Level	Count
GO:0005048	signal sequence binding	4	1
GO:0005215	transporter activity	1	1
GO:0005488	binding	1	1
GO:0008320	protein transmembrane transporter activity	3	1
GO:0022857	transmembrane transporter activity	2	1
GO:0022884	macromolecule transmembrane transporter activity	3	1
GO:0033218	amide binding	2	1
GO:0042277	peptide binding	3	1
GO:0043021	ribonucleoprotein complex binding	3	1
GO:0043022	ribosome binding	4	1
GO:0044877	protein-containing complex binding	2	1
GO:0140318	protein transporter activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	311	317	PF00089	0.460
CLV_NRD_NRD_1	313	315	PF00675	0.460
CLV_PCSK_KEX2_1	313	315	PF00082	0.460
CLV_PCSK_SKI1_1	112	116	PF00082	0.271
CLV_PCSK_SKI1_1	12	16	PF00082	0.432
CLV_PCSK_SKI1_1	234	238	PF00082	0.468
CLV_PCSK_SKI1_1	318	322	PF00082	0.566
CLV_PCSK_SKI1_1	68	72	PF00082	0.460
DEG_APCC_DBOX_1	238	246	PF00400	0.260
DEG_APCC_DBOX_1	317	325	PF00400	0.362
DEG_MDM2_SWIB_1	381	388	PF02201	0.297
DOC_CYCLIN_yCln2_LP_2	351	354	PF00134	0.277
DOC_MAPK_MEF2A_6	75	83	PF00069	0.260
DOC_PP1_RVXF_1	31	37	PF00149	0.519
DOC_PP2B_LxvP_1	351	354	PF13499	0.271
DOC_PP4_FxxP_1	237	240	PF00568	0.260
DOC_PP4_FxxP_1	264	267	PF00568	0.460
DOC_USP7_MATH_1	133	137	PF00917	0.312
DOC_USP7_MATH_1	410	414	PF00917	0.489
DOC_USP7_MATH_1	448	452	PF00917	0.260
DOC_USP7_MATH_1	85	89	PF00917	0.440
DOC_WW_Pin1_4	197	202	PF00397	0.260
DOC_WW_Pin1_4	81	86	PF00397	0.297
LIG_14-3-3_CanoR_1	33	43	PF00244	0.453
LIG_AP2alpha_2	62	64	PF02296	0.260
LIG_APCC_ABBA_1	439	444	PF00400	0.297
LIG_BIR_II_1	1	5	PF00653	0.543
LIG_BRCT_BRCA1_1	311	315	PF00533	0.271
LIG_BRCT_BRCA1_1	60	64	PF00533	0.260
LIG_EVH1_2	353	357	PF00568	0.327
LIG_FHA_1	225	231	PF00498	0.362
LIG_FHA_1	249	255	PF00498	0.315
LIG_FHA_1	296	302	PF00498	0.356
LIG_FHA_1	451	457	PF00498	0.297
LIG_FHA_2	125	131	PF00498	0.297
LIG_FHA_2	354	360	PF00498	0.275
LIG_FHA_2	75	81	PF00498	0.260
LIG_GBD_Chelix_1	160	168	PF00786	0.380
LIG_IRF3_LxIS_1	78	84	PF10401	0.260
LIG_LIR_Apic_2	137	143	PF02991	0.362
LIG_LIR_Apic_2	235	240	PF02991	0.260
LIG_LIR_Gen_1	116	126	PF02991	0.562
LIG_LIR_Gen_1	246	257	PF02991	0.227
LIG_LIR_Gen_1	305	315	PF02991	0.449
LIG_LIR_Gen_1	356	365	PF02991	0.261
LIG_LIR_Gen_1	382	393	PF02991	0.461
LIG_LIR_Gen_1	440	449	PF02991	0.297
LIG_LIR_Nem_3	193	199	PF02991	0.310
LIG_LIR_Nem_3	246	252	PF02991	0.227
LIG_LIR_Nem_3	305	310	PF02991	0.413
LIG_LIR_Nem_3	356	360	PF02991	0.288
LIG_LIR_Nem_3	382	388	PF02991	0.461
LIG_LIR_Nem_3	422	427	PF02991	0.460
LIG_LIR_Nem_3	430	434	PF02991	0.460
LIG_LIR_Nem_3	440	445	PF02991	0.297
LIG_LIR_Nem_3	475	481	PF02991	0.618
LIG_NRBOX	167	173	PF00104	0.312
LIG_NRBOX	320	326	PF00104	0.271
LIG_Pex14_2	260	264	PF04695	0.297
LIG_Pex14_2	369	373	PF04695	0.305
LIG_Pex14_2	381	385	PF04695	0.279
LIG_PTB_Apo_2	300	307	PF02174	0.297
LIG_PTB_Apo_2	314	321	PF02174	0.362
LIG_PTB_Apo_2	460	467	PF02174	0.297
LIG_PTB_Phospho_1	460	466	PF10480	0.356
LIG_SH2_CRK	478	482	PF00017	0.638
LIG_SH2_PTP2	424	427	PF00017	0.460
LIG_SH2_SRC	424	427	PF00017	0.460
LIG_SH2_STAP1	288	292	PF00017	0.477
LIG_SH2_STAP1	358	362	PF00017	0.378
LIG_SH2_STAT3	238	241	PF00017	0.260
LIG_SH2_STAT5	134	137	PF00017	0.297
LIG_SH2_STAT5	288	291	PF00017	0.486
LIG_SH2_STAT5	350	353	PF00017	0.277
LIG_SH2_STAT5	370	373	PF00017	0.222
LIG_SH2_STAT5	424	427	PF00017	0.460
LIG_SH3_3	237	243	PF00018	0.270
LIG_SUMO_SIM_par_1	292	299	PF11976	0.356
LIG_SUMO_SIM_par_1	455	460	PF11976	0.297
LIG_TRFH_1	349	353	PF08558	0.284
LIG_TYR_ITAM	463	481	PF00017	0.513
LIG_TYR_ITIM	347	352	PF00017	0.362
LIG_TYR_ITIM	476	481	PF00017	0.603
LIG_UBA3_1	14	23	PF00899	0.654
LIG_UBA3_1	167	174	PF00899	0.312
LIG_UBA3_1	8	12	PF00899	0.655
LIG_Vh1_VBS_1	373	391	PF01044	0.373
MOD_CK1_1	180	186	PF00069	0.517
MOD_CK1_1	200	206	PF00069	0.232
MOD_CK1_1	299	305	PF00069	0.297
MOD_CK2_1	124	130	PF00069	0.297
MOD_CK2_1	74	80	PF00069	0.260
MOD_GlcNHglycan	136	139	PF01048	0.484
MOD_GlcNHglycan	179	182	PF01048	0.314
MOD_GlcNHglycan	450	453	PF01048	0.297
MOD_GlcNHglycan	87	90	PF01048	0.387
MOD_GSK3_1	180	187	PF00069	0.474
MOD_GSK3_1	199	206	PF00069	0.289
MOD_GSK3_1	243	250	PF00069	0.380
MOD_GSK3_1	295	302	PF00069	0.330
MOD_GSK3_1	305	312	PF00069	0.249
MOD_GSK3_1	384	391	PF00069	0.497
MOD_GSK3_1	70	77	PF00069	0.264
MOD_GSK3_1	81	88	PF00069	0.305
MOD_N-GLC_1	243	248	PF02516	0.471
MOD_N-GLC_1	302	307	PF02516	0.373
MOD_N-GLC_1	58	63	PF02516	0.528
MOD_N-GLC_2	189	191	PF02516	0.297
MOD_NEK2_1	124	129	PF00069	0.297
MOD_NEK2_1	159	164	PF00069	0.340
MOD_NEK2_1	184	189	PF00069	0.298
MOD_NEK2_1	222	227	PF00069	0.271
MOD_NEK2_1	269	274	PF00069	0.460
MOD_NEK2_1	286	291	PF00069	0.460
MOD_NEK2_1	296	301	PF00069	0.297
MOD_NEK2_1	373	378	PF00069	0.365
MOD_NEK2_1	384	389	PF00069	0.460
MOD_NEK2_1	457	462	PF00069	0.297
MOD_NEK2_2	437	442	PF00069	0.297
MOD_NEK2_2	7	12	PF00069	0.657
MOD_PIKK_1	305	311	PF00454	0.297
MOD_PIKK_1	399	405	PF00454	0.568
MOD_PKA_2	410	416	PF00069	0.460
MOD_Plk_1	302	308	PF00069	0.373
MOD_Plk_4	159	165	PF00069	0.319
MOD_Plk_4	180	186	PF00069	0.460
MOD_Plk_4	190	196	PF00069	0.297
MOD_Plk_4	248	254	PF00069	0.254
MOD_Plk_4	296	302	PF00069	0.320
MOD_Plk_4	353	359	PF00069	0.277
MOD_Plk_4	437	443	PF00069	0.297
MOD_Plk_4	457	463	PF00069	0.168
MOD_Plk_4	74	80	PF00069	0.260
MOD_Plk_4	87	93	PF00069	0.308
MOD_ProDKin_1	197	203	PF00069	0.260
MOD_ProDKin_1	81	87	PF00069	0.297
TRG_DiLeu_BaLyEn_6	10	15	PF01217	0.579
TRG_DiLeu_BaLyEn_6	416	421	PF01217	0.471
TRG_ENDOCYTIC_2	349	352	PF00928	0.288
TRG_ENDOCYTIC_2	358	361	PF00928	0.232
TRG_ENDOCYTIC_2	370	373	PF00928	0.297
TRG_ENDOCYTIC_2	424	427	PF00928	0.460
TRG_ENDOCYTIC_2	465	468	PF00928	0.521
TRG_ENDOCYTIC_2	478	481	PF00928	0.655
TRG_ER_diArg_1	313	315	PF00400	0.260
TRG_ER_diLys_1	482	486	PF00400	0.623
TRG_Pf-PMV_PEXEL_1	231	235	PF00026	0.460
TRG_Pf-PMV_PEXEL_1	405	409	PF00026	0.260

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4G7	Leptomonas seymouri	91%	100%
A0A0S4INX1	Bodo saltans	74%	100%
A0A1X0NWC0	Trypanosomatidae	80%	100%
A0A3R7MTY8	Trypanosoma rangeli	81%	100%
A0A3S5H6J5	Leishmania donovani	96%	100%
A4HV14	Leishmania infantum	96%	100%
D0A7D4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	76%	100%
E9ANP9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O26134	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	36%	100%
O28377	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	30%	99%
O42965	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
O59442	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	35%	100%
P28541	Methanococcus vannielii	33%	100%
P28542	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	28%	100%
P32915	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	46%	100%
P38353	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	99%
P38377	Canis lupus familiaris	56%	100%
P38379	Pyrenomonas salina	54%	98%
P49978	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	27%	100%
P61619	Homo sapiens	56%	100%
P61620	Mus musculus	56%	100%
P61621	Rattus norvegicus	56%	100%
P78979	Yarrowia lipolytica (strain CLIB 122 / E 150)	47%	100%
P79088	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	46%	100%
Q25147	Halocynthia roretzi	55%	100%
Q2KHX4	Bos taurus	56%	100%
Q4QGX4	Leishmania major	96%	100%
Q54XK2	Dictyostelium discoideum	52%	100%
Q5EA68	Bos taurus	56%	100%
Q5NVM7	Pongo abelii	56%	100%
Q5R5L5	Pongo abelii	56%	100%
Q60175	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	33%	100%
Q6BN08	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	49%	100%
Q6CPY9	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	48%	100%
Q6FRY3	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	46%	100%
Q752H7	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	46%	100%
Q7T277	Dissostichus mawsoni	57%	100%
Q7T278	Harpagifer antarcticus	57%	100%
Q870W0	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	49%	100%
Q8AY31	Bovichtus variegatus	56%	100%
Q8AY32	Gadus ogac	57%	100%
Q8AY33	Boreogadus saida	56%	100%
Q8AY34	Hemitripterus americanus	57%	100%
Q8AY35	Notothenia angustata	57%	100%
Q8AY36	Pagothenia borchgrevinki	57%	100%
Q8U019	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	34%	100%
Q90YL4	Danio rerio	58%	100%
Q90ZM2	Danio rerio	57%	100%
Q96TW8	Wickerhamomyces anomalus	48%	100%
Q977V3	Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)	28%	99%
Q98SN8	Oncorhynchus mykiss	57%	100%
Q98SN9	Oncorhynchus mykiss	57%	100%
Q9H9S3	Homo sapiens	56%	100%
Q9HPB1	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	28%	99%
Q9JLR1	Mus musculus	56%	100%
Q9P8E3	Candida albicans (strain SC5314 / ATCC MYA-2876)	49%	100%
Q9UX84	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	28%	100%
Q9V1V8	Pyrococcus abyssi (strain GE5 / Orsay)	35%	100%
Q9YDD0	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	32%	100%
V5BBD8	Trypanosoma cruzi	80%	100%