tRNA pseudouridine(55) synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4H6H8_LEIBR

TriTrypDb:

LbrM.11.0300 , LBRM2903_110007900

Length:

721

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A4H6H8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6H8

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006400	tRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	278	280	PF00675	0.599
CLV_NRD_NRD_1	427	429	PF00675	0.373
CLV_NRD_NRD_1	645	647	PF00675	0.407
CLV_NRD_NRD_1	83	85	PF00675	0.571
CLV_PCSK_KEX2_1	278	280	PF00082	0.556
CLV_PCSK_KEX2_1	289	291	PF00082	0.635
CLV_PCSK_KEX2_1	522	524	PF00082	0.471
CLV_PCSK_KEX2_1	645	647	PF00082	0.407
CLV_PCSK_KEX2_1	83	85	PF00082	0.570
CLV_PCSK_PC1ET2_1	289	291	PF00082	0.635
CLV_PCSK_PC1ET2_1	522	524	PF00082	0.271
CLV_PCSK_PC7_1	274	280	PF00082	0.552
CLV_PCSK_PC7_1	641	647	PF00082	0.384
CLV_PCSK_SKI1_1	18	22	PF00082	0.341
CLV_PCSK_SKI1_1	180	184	PF00082	0.454
CLV_PCSK_SKI1_1	317	321	PF00082	0.663
DEG_APCC_DBOX_1	316	324	PF00400	0.589
DEG_MDM2_SWIB_1	182	189	PF02201	0.437
DEG_SPOP_SBC_1	11	15	PF00917	0.446
DEG_SPOP_SBC_1	302	306	PF00917	0.647
DOC_ANK_TNKS_1	306	313	PF00023	0.481
DOC_ANK_TNKS_1	536	543	PF00023	0.477
DOC_CKS1_1	636	641	PF01111	0.334
DOC_MAPK_DCC_7	252	261	PF00069	0.641
DOC_MAPK_FxFP_2	33	36	PF00069	0.528
DOC_MAPK_FxFP_2	462	465	PF00069	0.356
DOC_MAPK_gen_1	651	660	PF00069	0.339
DOC_MAPK_JIP1_4	543	549	PF00069	0.297
DOC_MAPK_MEF2A_6	252	261	PF00069	0.642
DOC_MAPK_MEF2A_6	531	538	PF00069	0.342
DOC_MAPK_MEF2A_6	690	697	PF00069	0.380
DOC_MAPK_MEF2A_6	702	710	PF00069	0.276
DOC_PP1_RVXF_1	603	610	PF00149	0.315
DOC_PP1_RVXF_1	644	651	PF00149	0.312
DOC_PP2B_LxvP_1	100	103	PF13499	0.630
DOC_PP4_FxxP_1	299	302	PF00568	0.498
DOC_PP4_FxxP_1	33	36	PF00568	0.477
DOC_PP4_FxxP_1	462	465	PF00568	0.356
DOC_USP7_MATH_1	116	120	PF00917	0.720
DOC_USP7_MATH_1	204	208	PF00917	0.522
DOC_USP7_MATH_1	241	245	PF00917	0.648
DOC_USP7_MATH_1	247	251	PF00917	0.595
DOC_USP7_MATH_1	302	306	PF00917	0.677
DOC_USP7_MATH_1	331	335	PF00917	0.569
DOC_USP7_MATH_1	410	414	PF00917	0.596
DOC_USP7_MATH_1	511	515	PF00917	0.490
DOC_USP7_MATH_1	594	598	PF00917	0.415
DOC_WW_Pin1_4	105	110	PF00397	0.504
DOC_WW_Pin1_4	161	166	PF00397	0.461
DOC_WW_Pin1_4	434	439	PF00397	0.338
DOC_WW_Pin1_4	474	479	PF00397	0.623
DOC_WW_Pin1_4	548	553	PF00397	0.353
DOC_WW_Pin1_4	635	640	PF00397	0.335
DOC_WW_Pin1_4	658	663	PF00397	0.386
DOC_WW_Pin1_4	689	694	PF00397	0.403
DOC_WW_Pin1_4	75	80	PF00397	0.465
LIG_14-3-3_CanoR_1	180	185	PF00244	0.380
LIG_14-3-3_CanoR_1	28	32	PF00244	0.433
LIG_14-3-3_CanoR_1	290	299	PF00244	0.578
LIG_14-3-3_CanoR_1	342	350	PF00244	0.529
LIG_14-3-3_CanoR_1	363	370	PF00244	0.524
LIG_14-3-3_CanoR_1	407	413	PF00244	0.624
LIG_14-3-3_CanoR_1	432	441	PF00244	0.447
LIG_14-3-3_CanoR_1	504	509	PF00244	0.522
LIG_14-3-3_CanoR_1	556	562	PF00244	0.419
LIG_14-3-3_CanoR_1	583	591	PF00244	0.375
LIG_14-3-3_CanoR_1	595	601	PF00244	0.446
LIG_14-3-3_CanoR_1	645	649	PF00244	0.411
LIG_14-3-3_CanoR_1	653	659	PF00244	0.370
LIG_APCC_ABBA_1	259	264	PF00400	0.605
LIG_APCC_ABBAyCdc20_2	681	687	PF00400	0.327
LIG_BIR_II_1	1	5	PF00653	0.666
LIG_BRCT_BRCA1_1	180	184	PF00533	0.297
LIG_BRCT_BRCA1_1	29	33	PF00533	0.418
LIG_BRCT_BRCA1_1	336	340	PF00533	0.333
LIG_BRCT_BRCA1_1	436	440	PF00533	0.434
LIG_Clathr_ClatBox_1	669	673	PF01394	0.343
LIG_eIF4E_1	146	152	PF01652	0.503
LIG_FHA_1	106	112	PF00498	0.613
LIG_FHA_1	171	177	PF00498	0.560
LIG_FHA_1	251	257	PF00498	0.581
LIG_FHA_1	344	350	PF00498	0.522
LIG_FHA_1	363	369	PF00498	0.439
LIG_FHA_1	588	594	PF00498	0.398
LIG_FHA_1	690	696	PF00498	0.370
LIG_FHA_1	701	707	PF00498	0.291
LIG_FHA_2	113	119	PF00498	0.700
LIG_FHA_2	197	203	PF00498	0.562
LIG_FHA_2	480	486	PF00498	0.647
LIG_FHA_2	556	562	PF00498	0.403
LIG_FHA_2	583	589	PF00498	0.378
LIG_FHA_2	61	67	PF00498	0.564
LIG_FHA_2	94	100	PF00498	0.710
LIG_LIR_Apic_2	296	302	PF02991	0.495
LIG_LIR_Apic_2	30	36	PF02991	0.454
LIG_LIR_Apic_2	375	379	PF02991	0.335
LIG_LIR_Gen_1	136	145	PF02991	0.398
LIG_LIR_Gen_1	181	191	PF02991	0.294
LIG_LIR_Gen_1	371	381	PF02991	0.258
LIG_LIR_Gen_1	503	513	PF02991	0.594
LIG_LIR_Nem_3	136	140	PF02991	0.533
LIG_LIR_Nem_3	181	187	PF02991	0.295
LIG_LIR_Nem_3	371	377	PF02991	0.254
LIG_LIR_Nem_3	503	508	PF02991	0.658
LIG_NRBOX	694	700	PF00104	0.336
LIG_Pex14_2	182	186	PF04695	0.452
LIG_Rb_LxCxE_1	558	580	PF01857	0.278
LIG_Rb_pABgroove_1	27	35	PF01858	0.405
LIG_SH2_CRK	370	374	PF00017	0.304
LIG_SH2_CRK	604	608	PF00017	0.309
LIG_SH2_NCK_1	370	374	PF00017	0.337
LIG_SH2_PTP2	376	379	PF00017	0.250
LIG_SH2_SRC	262	265	PF00017	0.489
LIG_SH2_SRC	374	377	PF00017	0.327
LIG_SH2_STAP1	133	137	PF00017	0.557
LIG_SH2_STAP1	167	171	PF00017	0.523
LIG_SH2_STAP1	370	374	PF00017	0.319
LIG_SH2_STAT5	193	196	PF00017	0.517
LIG_SH2_STAT5	232	235	PF00017	0.452
LIG_SH2_STAT5	262	265	PF00017	0.505
LIG_SH2_STAT5	359	362	PF00017	0.409
LIG_SH2_STAT5	376	379	PF00017	0.280
LIG_SH2_STAT5	380	383	PF00017	0.359
LIG_SH2_STAT5	422	425	PF00017	0.449
LIG_SH2_STAT5	679	682	PF00017	0.346
LIG_SH2_STAT5	85	88	PF00017	0.524
LIG_SH3_1	659	665	PF00018	0.258
LIG_SH3_3	108	114	PF00018	0.802
LIG_SH3_3	455	461	PF00018	0.358
LIG_SH3_3	462	468	PF00018	0.420
LIG_SH3_3	469	475	PF00018	0.669
LIG_SH3_3	505	511	PF00018	0.386
LIG_SH3_3	659	665	PF00018	0.364
LIG_SUMO_SIM_par_1	477	485	PF11976	0.470
LIG_SUMO_SIM_par_1	545	551	PF11976	0.256
LIG_SUMO_SIM_par_1	668	674	PF11976	0.397
LIG_TRAF2_1	282	285	PF00917	0.496
LIG_TRAF2_1	450	453	PF00917	0.301
LIG_TRAF2_1	482	485	PF00917	0.571
LIG_TYR_ITIM	191	196	PF00017	0.253
LIG_TYR_ITIM	368	373	PF00017	0.308
LIG_WRC_WIRS_1	446	451	PF05994	0.447
LIG_WW_3	36	40	PF00397	0.544
MOD_CDC14_SPxK_1	551	554	PF00782	0.381
MOD_CDK_SPK_2	75	80	PF00069	0.463
MOD_CDK_SPxK_1	548	554	PF00069	0.360
MOD_CDK_SPxK_1	635	641	PF00069	0.339
MOD_CK1_1	236	242	PF00069	0.553
MOD_CK1_1	250	256	PF00069	0.632
MOD_CK1_1	329	335	PF00069	0.588
MOD_CK1_1	47	53	PF00069	0.719
MOD_CK1_1	490	496	PF00069	0.668
MOD_CK1_1	54	60	PF00069	0.633
MOD_CK2_1	112	118	PF00069	0.760
MOD_CK2_1	196	202	PF00069	0.552
MOD_CK2_1	269	275	PF00069	0.447
MOD_CK2_1	447	453	PF00069	0.374
MOD_CK2_1	479	485	PF00069	0.646
MOD_Cter_Amidation	276	279	PF01082	0.450
MOD_GlcNHglycan	1	4	PF01048	0.706
MOD_GlcNHglycan	128	131	PF01048	0.552
MOD_GlcNHglycan	328	331	PF01048	0.592
MOD_GlcNHglycan	489	492	PF01048	0.657
MOD_GlcNHglycan	514	517	PF01048	0.397
MOD_GlcNHglycan	527	530	PF01048	0.329
MOD_GlcNHglycan	53	56	PF01048	0.748
MOD_GlcNHglycan	569	572	PF01048	0.378
MOD_GSK3_1	112	119	PF00069	0.714
MOD_GSK3_1	166	173	PF00069	0.488
MOD_GSK3_1	178	185	PF00069	0.372
MOD_GSK3_1	236	243	PF00069	0.564
MOD_GSK3_1	293	300	PF00069	0.677
MOD_GSK3_1	47	54	PF00069	0.683
MOD_GSK3_1	500	507	PF00069	0.592
MOD_GSK3_1	569	576	PF00069	0.388
MOD_GSK3_1	654	661	PF00069	0.376
MOD_GSK3_1	671	678	PF00069	0.325
MOD_N-GLC_1	178	183	PF02516	0.420
MOD_N-GLC_1	196	201	PF02516	0.341
MOD_N-GLC_1	361	366	PF02516	0.513
MOD_N-GLC_1	432	437	PF02516	0.485
MOD_NEK2_1	182	187	PF00069	0.378
MOD_NEK2_1	196	201	PF00069	0.555
MOD_NEK2_1	269	274	PF00069	0.409
MOD_NEK2_1	27	32	PF00069	0.344
MOD_NEK2_1	326	331	PF00069	0.608
MOD_NEK2_1	654	659	PF00069	0.355
MOD_NEK2_1	688	693	PF00069	0.371
MOD_NEK2_2	587	592	PF00069	0.415
MOD_PIKK_1	432	438	PF00454	0.339
MOD_PIKK_1	577	583	PF00454	0.416
MOD_PIKK_1	671	677	PF00454	0.318
MOD_PK_1	504	510	PF00069	0.508
MOD_PK_1	630	636	PF00069	0.381
MOD_PKA_2	27	33	PF00069	0.324
MOD_PKA_2	326	332	PF00069	0.616
MOD_PKA_2	362	368	PF00069	0.552
MOD_PKA_2	402	408	PF00069	0.633
MOD_PKA_2	497	503	PF00069	0.509
MOD_PKA_2	555	561	PF00069	0.507
MOD_PKA_2	582	588	PF00069	0.365
MOD_PKA_2	594	600	PF00069	0.412
MOD_PKA_2	644	650	PF00069	0.397
MOD_Plk_1	178	184	PF00069	0.432
MOD_Plk_1	57	63	PF00069	0.616
MOD_Plk_1	577	583	PF00069	0.383
MOD_Plk_1	587	593	PF00069	0.390
MOD_Plk_1	630	636	PF00069	0.286
MOD_Plk_2-3	555	561	PF00069	0.456
MOD_Plk_4	133	139	PF00069	0.533
MOD_Plk_4	205	211	PF00069	0.480
MOD_Plk_4	241	247	PF00069	0.484
MOD_Plk_4	27	33	PF00069	0.325
MOD_Plk_4	331	337	PF00069	0.568
MOD_Plk_4	368	374	PF00069	0.336
MOD_Plk_4	490	496	PF00069	0.462
MOD_Plk_4	675	681	PF00069	0.361
MOD_ProDKin_1	105	111	PF00069	0.506
MOD_ProDKin_1	161	167	PF00069	0.462
MOD_ProDKin_1	434	440	PF00069	0.343
MOD_ProDKin_1	474	480	PF00069	0.626
MOD_ProDKin_1	548	554	PF00069	0.360
MOD_ProDKin_1	635	641	PF00069	0.339
MOD_ProDKin_1	658	664	PF00069	0.389
MOD_ProDKin_1	689	695	PF00069	0.398
MOD_ProDKin_1	75	81	PF00069	0.461
MOD_SUMO_for_1	210	213	PF00179	0.538
MOD_SUMO_rev_2	483	489	PF00179	0.461
MOD_SUMO_rev_2	597	601	PF00179	0.509
TRG_DiLeu_BaEn_2	540	546	PF01217	0.383
TRG_DiLeu_BaLyEn_6	650	655	PF01217	0.489
TRG_ENDOCYTIC_2	146	149	PF00928	0.347
TRG_ENDOCYTIC_2	193	196	PF00928	0.517
TRG_ENDOCYTIC_2	370	373	PF00928	0.301
TRG_ENDOCYTIC_2	374	377	PF00928	0.327
TRG_ENDOCYTIC_2	427	430	PF00928	0.335
TRG_ENDOCYTIC_2	604	607	PF00928	0.315
TRG_ER_diArg_1	225	228	PF00400	0.441
TRG_ER_diArg_1	650	653	PF00400	0.374
TRG_ER_diArg_1	82	84	PF00400	0.545
TRG_NES_CRM1_1	212	224	PF08389	0.509
TRG_NES_CRM1_1	704	719	PF08389	0.359
TRG_NLS_Bipartite_1	278	293	PF00514	0.481
TRG_NLS_MonoExtC_3	288	294	PF00514	0.591
TRG_Pf-PMV_PEXEL_1	537	541	PF00026	0.492
TRG_Pf-PMV_PEXEL_1	612	616	PF00026	0.381

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HW55	Leptomonas seymouri	66%	96%
A0A0S4IV39	Bodo saltans	37%	90%
A0A1X0NVE2	Trypanosomatidae	46%	100%
A0A3S5H6I6	Leishmania donovani	83%	100%
A0A422MV99	Trypanosoma rangeli	45%	100%
A4HUW4	Leishmania infantum	83%	100%
D0A7I9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9ANJ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
Q4QH27	Leishmania major	84%	100%
V5BW97	Trypanosoma cruzi	45%	100%