Leishmanolysin

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Leishmanolysin

Gene product:

GP63, leishmanolysin (fragment)

Species:

Leishmania braziliensis

UniProt:

A4H6E7_LEIBR

TriTrypDb:

LbrM.10.1710

Length:

305

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 10, no: 34
NetGPI	no	yes: 0, no: 44

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	45
GO:0110165	cellular anatomical entity	1	45

Expansion

Sequence features

A4H6E7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6E7

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	45
GO:0006807	nitrogen compound metabolic process	2	45
GO:0007155	cell adhesion	2	45
GO:0008152	metabolic process	1	45
GO:0009987	cellular process	1	45
GO:0019538	protein metabolic process	3	45
GO:0043170	macromolecule metabolic process	3	45
GO:0044238	primary metabolic process	2	45
GO:0071704	organic substance metabolic process	2	45
GO:1901564	organonitrogen compound metabolic process	3	45

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	45
GO:0004175	endopeptidase activity	4	45
GO:0004222	metalloendopeptidase activity	5	45
GO:0005488	binding	1	45
GO:0008233	peptidase activity	3	45
GO:0008237	metallopeptidase activity	4	45
GO:0016787	hydrolase activity	2	45
GO:0043167	ion binding	2	45
GO:0043169	cation binding	3	45
GO:0046872	metal ion binding	4	45
GO:0140096	catalytic activity, acting on a protein	2	45

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	210	212	PF00675	0.516
CLV_PCSK_KEX2_1	210	212	PF00082	0.506
CLV_PCSK_SKI1_1	137	141	PF00082	0.490
CLV_PCSK_SKI1_1	59	63	PF00082	0.574
CLV_PCSK_SKI1_1	83	87	PF00082	0.509
DEG_Nend_UBRbox_2	1	3	PF02207	0.463
DOC_CKS1_1	229	234	PF01111	0.408
DOC_CYCLIN_yCln2_LP_2	143	149	PF00134	0.392
DOC_PP2B_LxvP_1	143	146	PF13499	0.383
DOC_PP4_FxxP_1	93	96	PF00568	0.404
DOC_USP7_MATH_1	296	300	PF00917	0.383
DOC_WW_Pin1_4	228	233	PF00397	0.364
DOC_WW_Pin1_4	298	303	PF00397	0.387
LIG_14-3-3_CanoR_1	181	188	PF00244	0.387
LIG_14-3-3_CanoR_1	227	232	PF00244	0.344
LIG_14-3-3_CanoR_1	297	302	PF00244	0.394
LIG_AP2alpha_1	240	244	PF02296	0.244
LIG_APCC_ABBA_1	158	163	PF00400	0.365
LIG_APCC_ABBA_1	280	285	PF00400	0.377
LIG_BRCT_BRCA1_1	183	187	PF00533	0.409
LIG_BRCT_BRCA1_1	71	75	PF00533	0.311
LIG_FHA_1	155	161	PF00498	0.351
LIG_FHA_1	214	220	PF00498	0.369
LIG_FHA_1	221	227	PF00498	0.366
LIG_FHA_1	26	32	PF00498	0.361
LIG_FHA_1	56	62	PF00498	0.375
LIG_FHA_1	77	83	PF00498	0.347
LIG_LIR_Apic_2	109	114	PF02991	0.367
LIG_LIR_Apic_2	209	215	PF02991	0.386
LIG_LIR_Apic_2	228	232	PF02991	0.344
LIG_LIR_Gen_1	115	123	PF02991	0.387
LIG_LIR_Gen_1	235	245	PF02991	0.351
LIG_LIR_Gen_1	279	287	PF02991	0.409
LIG_LIR_Nem_3	115	121	PF02991	0.395
LIG_LIR_Nem_3	235	240	PF02991	0.342
LIG_LIR_Nem_3	249	254	PF02991	0.194
LIG_LIR_Nem_3	279	283	PF02991	0.409
LIG_LIR_Nem_3	8	12	PF02991	0.333
LIG_MLH1_MIPbox_1	72	76	PF16413	0.277
LIG_Pex14_1	200	204	PF04695	0.411
LIG_Pex14_2	240	244	PF04695	0.397
LIG_Pex14_2	71	75	PF04695	0.361
LIG_PTB_Apo_2	238	245	PF02174	0.373
LIG_PTB_Apo_2	3	10	PF02174	0.371
LIG_SH2_CRK	111	115	PF00017	0.381
LIG_SH2_CRK	153	157	PF00017	0.293
LIG_SH2_CRK	212	216	PF00017	0.356
LIG_SH2_CRK	229	233	PF00017	0.317
LIG_SH2_GRB2like	239	242	PF00017	0.194
LIG_SH2_SRC	118	121	PF00017	0.311
LIG_SH2_STAP1	248	252	PF00017	0.200
LIG_SH2_STAT5	118	121	PF00017	0.408
LIG_SH2_STAT5	12	15	PF00017	0.334
LIG_SH2_STAT5	153	156	PF00017	0.302
LIG_SH2_STAT5	236	239	PF00017	0.323
LIG_SH2_STAT5	254	257	PF00017	0.388
LIG_SH3_3	14	20	PF00018	0.394
LIG_SH3_3	290	296	PF00018	0.395
LIG_TYR_ITIM	116	121	PF00017	0.327
MOD_CDK_SPK_2	300	305	PF00069	0.410
MOD_CDK_SPxxK_3	298	305	PF00069	0.377
MOD_CK1_1	129	135	PF00069	0.351
MOD_CK1_1	15	21	PF00069	0.384
MOD_CK1_1	281	287	PF00069	0.356
MOD_CK2_1	169	175	PF00069	0.333
MOD_GlcNHglycan	126	129	PF01048	0.559
MOD_GlcNHglycan	147	150	PF01048	0.545
MOD_GlcNHglycan	248	251	PF01048	0.574
MOD_GSK3_1	228	235	PF00069	0.378
MOD_GSK3_1	296	303	PF00069	0.381
MOD_GSK3_1	55	62	PF00069	0.352
MOD_N-GLC_1	181	186	PF02516	0.489
MOD_N-GLC_1	195	200	PF02516	0.572
MOD_N-GLC_1	205	210	PF02516	0.529
MOD_N-GLC_1	240	245	PF02516	0.590
MOD_N-GLC_1	297	302	PF02516	0.582
MOD_N-GLC_1	98	103	PF02516	0.541
MOD_N-GLC_2	190	192	PF02516	0.601
MOD_NEK2_1	240	245	PF00069	0.399
MOD_NEK2_1	69	74	PF00069	0.344
MOD_NEK2_1	76	81	PF00069	0.310
MOD_NEK2_2	70	75	PF00069	0.397
MOD_PIKK_1	195	201	PF00454	0.363
MOD_PK_1	278	284	PF00069	0.227
MOD_PKA_2	296	302	PF00069	0.375
MOD_PKB_1	211	219	PF00069	0.232
MOD_Plk_1	169	175	PF00069	0.332
MOD_Plk_1	195	201	PF00069	0.390
MOD_Plk_1	240	246	PF00069	0.395
MOD_Plk_1	278	284	PF00069	0.389
MOD_Plk_1	98	104	PF00069	0.381
MOD_Plk_4	232	238	PF00069	0.384
MOD_Plk_4	240	246	PF00069	0.384
MOD_Plk_4	70	76	PF00069	0.359
MOD_Plk_4	99	105	PF00069	0.371
MOD_ProDKin_1	228	234	PF00069	0.364
MOD_ProDKin_1	298	304	PF00069	0.387
MOD_SUMO_for_1	139	142	PF00179	0.296
TRG_ENDOCYTIC_2	118	121	PF00928	0.327
TRG_ENDOCYTIC_2	153	156	PF00928	0.289
TRG_ENDOCYTIC_2	248	251	PF00928	0.348
TRG_ENDOCYTIC_2	254	257	PF00928	0.349
TRG_ER_diArg_1	210	212	PF00400	0.379
TRG_ER_diArg_1	218	221	PF00400	0.385

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IFV2	Leptomonas seymouri	41%	100%
A0A1X0NFB0	Trypanosomatidae	37%	75%
A0A1X0NFK9	Trypanosomatidae	38%	100%
A0A1X0NFZ9	Trypanosomatidae	33%	66%
A0A1X0NGN3	Trypanosomatidae	35%	73%
A0A1X0NHP4	Trypanosomatidae	33%	76%
A0A1X0NIJ6	Trypanosomatidae	33%	74%
A0A1X0NJC6	Trypanosomatidae	36%	71%
A0A1X0NQJ5	Trypanosomatidae	45%	66%
A0A1X0NQM4	Trypanosomatidae	43%	66%
A0A1X0NQN3	Trypanosomatidae	39%	70%
A0A1X0NQU3	Trypanosomatidae	51%	80%
A0A1X0NR02	Trypanosomatidae	45%	89%
A0A1X0NTJ1	Trypanosomatidae	33%	75%
A0A1X0NWK7	Trypanosomatidae	38%	76%
A0A1X0NY94	Trypanosomatidae	41%	72%
A0A1X0NZS0	Trypanosomatidae	30%	77%
A0A1X0P5K2	Trypanosomatidae	42%	69%
A0A3R7JS09	Trypanosoma rangeli	34%	88%
A0A3R7JUH9	Trypanosoma rangeli	43%	89%
A0A3R7MTS2	Trypanosoma rangeli	41%	67%
A0A3R7N7I3	Trypanosoma rangeli	46%	75%
A0A422MNW0	Trypanosoma rangeli	43%	75%
A0A422MP17	Trypanosoma rangeli	44%	69%
A0A422MR94	Trypanosoma rangeli	43%	100%
A0A422MUP1	Trypanosoma rangeli	44%	89%
A0A422MWD4	Trypanosoma rangeli	36%	100%
A0A422N0V4	Trypanosoma rangeli	43%	71%
A0A422NX20	Trypanosoma rangeli	40%	71%
A4H629	Leishmania braziliensis	91%	100%
A4H636	Leishmania braziliensis	66%	68%
A4H6E4	Leishmania braziliensis	70%	100%
D0A7W4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	69%
E8NHL1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	73%	100%
V5A303	Trypanosoma cruzi	44%	73%
V5ABN5	Trypanosoma cruzi	34%	100%
V5ALP9	Trypanosoma cruzi	35%	100%
V5AN34	Trypanosoma cruzi	44%	73%
V5ANT4	Trypanosoma cruzi	43%	94%
V5APB3	Trypanosoma cruzi	38%	95%
V5BAB0	Trypanosoma cruzi	38%	86%
V5BD39	Trypanosoma cruzi	44%	69%
V5CHU5	Trypanosoma cruzi	43%	95%