LeishMANIAdb
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Leishmanolysin

Quick info Annotations Function or PPIs Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Leishmanolysin
Gene product:
GP63, leishmanolysin
Species:
Leishmania braziliensis
UniProt:
A4H6E4_LEIBR
TriTrypDb:
LbrM.10.1680 , LBRM2903_100012600 *
Length:
401

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

LeishMANIAdb interaction annotations

by homology
Contact email: albert.descoteaux@iaf.inrs.ca
Publication title: The host cell secretory pathway mediates the export of Leishmania virulence factors out of the parasitophorous vacuole
Publication 1st author(s): Amandine Isnard
Publication Identifier(s): 25826301
Host organism: -1
Interaction detection method(s): protease assay
Interaction type: physical association
Identification method participant A: monoclonal antibody western blot
Identification method participant B: monoclonal antibody western blot
ID(s) interactor A: P05627
ID(s) interactor B: P08148
Taxid interactor A: Mus musculus
Taxid interactor B: Leishmania major
Biological role(s) interactor A: enzyme
Biological role(s) interactor B: enzyme target
Experimental role(s) interactor A: neutral component

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 2
Forrest at al. (procyclic) no yes: 2
Silverman et al. no yes: 0
Pissara et al. no yes: 45
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 5
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 0
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 76, no: 66
NetGPI no yes: 0, no: 142
Cellular components
Term Name Level Count
GO:0016020 membrane 2 143
GO:0110165 cellular anatomical entity 1 143
GO:0005737 cytoplasm 2 4
GO:0018995 host cellular component 2 3
GO:0033643 host cell part 3 3
GO:0033646 host intracellular part 4 3
GO:0033647 host intracellular organelle 5 3
GO:0033648 host intracellular membrane-bounded organelle 6 3
GO:0042025 host cell nucleus 7 3

Expansion

Sequence features

A4H6E4
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6E4

Function

Biological processes
Term Name Level Count
GO:0006508 proteolysis 4 143
GO:0006807 nitrogen compound metabolic process 2 143
GO:0007155 cell adhesion 2 143
GO:0008152 metabolic process 1 143
GO:0009987 cellular process 1 143
GO:0019538 protein metabolic process 3 143
GO:0043170 macromolecule metabolic process 3 143
GO:0044238 primary metabolic process 2 143
GO:0071704 organic substance metabolic process 2 143
GO:1901564 organonitrogen compound metabolic process 3 143
GO:0009966 regulation of signal transduction 4 3
GO:0010646 regulation of cell communication 4 3
GO:0010749 regulation of nitric oxide mediated signal transduction 6 3
GO:0023051 regulation of signaling 3 3
GO:0035821 modulation of process of another organism 2 3
GO:0044003 modulation by symbiont of host process 3 3
GO:0044068 modulation by symbiont of host cellular process 4 3
GO:0044081 modulation by symbiont of host nitric oxide-mediated signal transduction 5 3
GO:0044403 biological process involved in symbiotic interaction 2 3
GO:0044419 biological process involved in interspecies interaction between organisms 1 3
GO:0044501 modulation of signal transduction in another organism 3 3
GO:0048583 regulation of response to stimulus 3 3
GO:0050789 regulation of biological process 2 3
GO:0050794 regulation of cellular process 3 3
GO:0051701 biological process involved in interaction with host 3 3
GO:0052027 modulation by symbiont of host signal transduction pathway 4 3
GO:0065007 biological regulation 1 3
GO:0075130 modulation by symbiont of host protein kinase-mediated signal transduction 5 3
GO:1902531 regulation of intracellular signal transduction 5 3
Molecular functions
Term Name Level Count
GO:0003824 catalytic activity 1 143
GO:0004175 endopeptidase activity 4 143
GO:0004222 metalloendopeptidase activity 5 143
GO:0005488 binding 1 143
GO:0008233 peptidase activity 3 143
GO:0008237 metallopeptidase activity 4 143
GO:0016787 hydrolase activity 2 143
GO:0043167 ion binding 2 143
GO:0043169 cation binding 3 143
GO:0046872 metal ion binding 4 143
GO:0140096 catalytic activity, acting on a protein 2 143
GO:0008047 enzyme activator activity 3 3
GO:0008160 protein tyrosine phosphatase activator activity 6 3
GO:0019208 phosphatase regulator activity 3 3
GO:0019211 phosphatase activator activity 4 3
GO:0019888 protein phosphatase regulator activity 4 3
GO:0030234 enzyme regulator activity 2 3
GO:0072542 protein phosphatase activator activity 5 3
GO:0098772 molecular function regulator activity 1 3
GO:0140677 molecular function activator activity 2 3

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_C14_Caspase3-7 47 51 PF00656 0.293
CLV_PCSK_SKI1_1 153 157 PF00082 0.437
CLV_PCSK_SKI1_1 211 215 PF00082 0.486
CLV_PCSK_SKI1_1 254 258 PF00082 0.473
CLV_PCSK_SKI1_1 75 79 PF00082 0.549
DEG_Nend_UBRbox_2 1 3 PF02207 0.133
DEG_SCF_FBW7_1 349 356 PF00400 0.335
DOC_CYCLIN_yCln2_LP_2 159 165 PF00134 0.351
DOC_PP1_RVXF_1 262 268 PF00149 0.146
DOC_PP2B_LxvP_1 159 162 PF13499 0.342
DOC_PP4_FxxP_1 9 12 PF00568 0.282
DOC_USP7_MATH_1 145 149 PF00917 0.267
DOC_USP7_MATH_1 353 357 PF00917 0.256
DOC_WW_Pin1_4 113 118 PF00397 0.252
DOC_WW_Pin1_4 287 292 PF00397 0.342
DOC_WW_Pin1_4 349 354 PF00397 0.327
DOC_WW_Pin1_4 64 69 PF00397 0.337
LIG_14-3-3_CanoR_1 211 220 PF00244 0.361
LIG_14-3-3_CanoR_1 229 237 PF00244 0.252
LIG_14-3-3_CanoR_1 239 249 PF00244 0.280
LIG_14-3-3_CanoR_1 254 261 PF00244 0.255
LIG_14-3-3_CanoR_1 264 268 PF00244 0.291
LIG_14-3-3_CanoR_1 333 339 PF00244 0.353
LIG_APCC_ABBA_1 174 179 PF00400 0.312
LIG_APCC_ABBA_1 296 301 PF00400 0.378
LIG_APCC_ABBA_1 89 94 PF00400 0.354
LIG_BRCT_BRCA1_1 199 203 PF00533 0.366
LIG_BRCT_BRCA1_1 362 366 PF00533 0.323
LIG_Clathr_ClatBox_1 388 392 PF01394 0.228
LIG_FHA_1 15 21 PF00498 0.267
LIG_FHA_1 171 177 PF00498 0.301
LIG_FHA_1 335 341 PF00498 0.322
LIG_FHA_1 362 368 PF00498 0.315
LIG_FHA_1 6 12 PF00498 0.267
LIG_FHA_1 76 82 PF00498 0.337
LIG_FHA_2 186 192 PF00498 0.301
LIG_FHA_2 241 247 PF00498 0.271
LIG_FHA_2 290 296 PF00498 0.255
LIG_FHA_2 350 356 PF00498 0.297
LIG_FHA_2 45 51 PF00498 0.259
LIG_FHA_2 88 94 PF00498 0.315
LIG_GBD_Chelix_1 81 89 PF00786 0.429
LIG_LIR_Apic_2 108 112 PF02991 0.370
LIG_LIR_Apic_2 125 130 PF02991 0.290
LIG_LIR_Apic_2 243 248 PF02991 0.266
LIG_LIR_Apic_2 8 12 PF02991 0.311
LIG_LIR_Gen_1 131 139 PF02991 0.363
LIG_LIR_Gen_1 295 301 PF02991 0.366
LIG_LIR_Gen_1 376 385 PF02991 0.338
LIG_LIR_Gen_1 67 77 PF02991 0.334
LIG_LIR_Gen_1 90 98 PF02991 0.276
LIG_LIR_Nem_3 131 137 PF02991 0.365
LIG_LIR_Nem_3 23 28 PF02991 0.298
LIG_LIR_Nem_3 251 256 PF02991 0.285
LIG_LIR_Nem_3 266 270 PF02991 0.274
LIG_LIR_Nem_3 295 299 PF02991 0.365
LIG_LIR_Nem_3 319 325 PF02991 0.314
LIG_LIR_Nem_3 376 380 PF02991 0.311
LIG_LIR_Nem_3 67 72 PF02991 0.325
LIG_LIR_Nem_3 90 95 PF02991 0.277
LIG_Pex14_1 216 220 PF04695 0.309
LIG_PTB_Apo_2 19 26 PF02174 0.327
LIG_SH2_CRK 127 131 PF00017 0.275
LIG_SH2_CRK 169 173 PF00017 0.257
LIG_SH2_CRK 270 274 PF00017 0.332
LIG_SH2_CRK 322 326 PF00017 0.365
LIG_SH2_CRK 69 73 PF00017 0.367
LIG_SH2_PTP2 377 380 PF00017 0.376
LIG_SH2_SRC 245 248 PF00017 0.307
LIG_SH2_SRC 377 380 PF00017 0.295
LIG_SH2_STAP1 338 342 PF00017 0.320
LIG_SH2_STAP1 69 73 PF00017 0.369
LIG_SH2_STAT3 338 341 PF00017 0.305
LIG_SH2_STAT5 169 172 PF00017 0.227
LIG_SH2_STAT5 252 255 PF00017 0.295
LIG_SH2_STAT5 270 273 PF00017 0.286
LIG_SH2_STAT5 28 31 PF00017 0.272
LIG_SH2_STAT5 348 351 PF00017 0.337
LIG_SH2_STAT5 371 374 PF00017 0.363
LIG_SH2_STAT5 377 380 PF00017 0.343
LIG_SH3_3 30 36 PF00018 0.354
LIG_SUMO_SIM_anti_2 93 100 PF11976 0.330
MOD_CDK_SPxxK_3 287 294 PF00069 0.151
MOD_CK1_1 115 121 PF00069 0.194
MOD_CK1_1 241 247 PF00069 0.292
MOD_CK1_1 287 293 PF00069 0.282
MOD_CK1_1 297 303 PF00069 0.285
MOD_CK1_1 31 37 PF00069 0.353
MOD_CK2_1 185 191 PF00069 0.287
MOD_CK2_1 289 295 PF00069 0.325
MOD_CK2_1 349 355 PF00069 0.319
MOD_CK2_1 373 379 PF00069 0.305
MOD_GlcNHglycan 143 146 PF01048 0.528
MOD_GlcNHglycan 163 166 PF01048 0.477
MOD_GlcNHglycan 345 348 PF01048 0.544
MOD_GSK3_1 137 144 PF00069 0.270
MOD_GSK3_1 14 21 PF00069 0.313
MOD_GSK3_1 220 227 PF00069 0.219
MOD_GSK3_1 285 292 PF00069 0.239
MOD_GSK3_1 349 356 PF00069 0.282
MOD_GSK3_1 44 51 PF00069 0.228
MOD_N-GLC_1 211 216 PF02516 0.503
MOD_N-GLC_1 221 226 PF02516 0.509
MOD_N-GLC_2 206 208 PF02516 0.501
MOD_NEK2_1 137 142 PF00069 0.283
MOD_NEK2_1 256 261 PF00069 0.328
MOD_NEK2_1 299 304 PF00069 0.242
MOD_NEK2_1 48 53 PF00069 0.252
MOD_NEK2_1 73 78 PF00069 0.311
MOD_NEK2_1 87 92 PF00069 0.306
MOD_PIKK_1 211 217 PF00454 0.345
MOD_PKA_2 228 234 PF00069 0.247
MOD_PKA_2 238 244 PF00069 0.261
MOD_PKA_2 263 269 PF00069 0.309
MOD_Plk_1 211 217 PF00069 0.356
MOD_Plk_1 257 263 PF00069 0.305
MOD_Plk_1 294 300 PF00069 0.271
MOD_Plk_1 87 93 PF00069 0.305
MOD_Plk_4 115 121 PF00069 0.292
MOD_Plk_4 248 254 PF00069 0.336
MOD_Plk_4 367 373 PF00069 0.289
MOD_Plk_4 87 93 PF00069 0.309
MOD_ProDKin_1 113 119 PF00069 0.252
MOD_ProDKin_1 287 293 PF00069 0.342
MOD_ProDKin_1 349 355 PF00069 0.327
MOD_ProDKin_1 64 70 PF00069 0.337
MOD_SUMO_for_1 155 158 PF00179 0.239
TRG_ENDOCYTIC_2 169 172 PF00928 0.250
TRG_ENDOCYTIC_2 270 273 PF00928 0.318
TRG_ENDOCYTIC_2 322 325 PF00928 0.350
TRG_ENDOCYTIC_2 377 380 PF00928 0.267
TRG_ENDOCYTIC_2 69 72 PF00928 0.344
TRG_NES_CRM1_1 88 100 PF08389 0.308
TRG_Pf-PMV_PEXEL_1 211 215 PF00026 0.548

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0S4IS53 Bodo saltans 36% 83%
A0A1X0NDK2 Trypanosomatidae 32% 69%
A0A1X0NDK9 Trypanosomatidae 29% 82%
A0A1X0NE71 Trypanosomatidae 33% 79%
A0A1X0NES0 Trypanosomatidae 33% 100%
A0A1X0NET7 Trypanosomatidae 35% 78%
A0A1X0NF32 Trypanosomatidae 33% 85%
A0A1X0NF66 Trypanosomatidae 31% 67%
A0A1X0NFJ0 Trypanosomatidae 28% 76%
A0A1X0NFZ9 Trypanosomatidae 34% 87%
A0A1X0NGN3 Trypanosomatidae 37% 97%
A0A1X0NGZ3 Trypanosomatidae 35% 67%
A0A1X0NHP4 Trypanosomatidae 31% 100%
A0A1X0NHP6 Trypanosomatidae 34% 76%
A0A1X0NHQ6 Trypanosomatidae 32% 78%
A0A1X0NIJ6 Trypanosomatidae 35% 97%
A0A1X0NIZ3 Trypanosomatidae 32% 71%
A0A1X0NJC6 Trypanosomatidae 34% 94%
A0A1X0NKW9 Trypanosomatidae 29% 79%
A0A1X0NMJ8 Trypanosomatidae 32% 70%
A0A1X0NMK3 Trypanosomatidae 30% 78%
A0A1X0NMK7 Trypanosomatidae 40% 77%
A0A1X0NQ02 Trypanosomatidae 29% 100%
A0A1X0NQJ5 Trypanosomatidae 35% 87%
A0A1X0NQM4 Trypanosomatidae 37% 87%
A0A1X0NQN3 Trypanosomatidae 38% 92%
A0A1X0NQU3 Trypanosomatidae 39% 100%
A0A1X0NQW6 Trypanosomatidae 34% 70%
A0A1X0NR02 Trypanosomatidae 38% 100%
A0A1X0NRY8 Trypanosomatidae 38% 73%
A0A1X0NTJ1 Trypanosomatidae 35% 99%
A0A1X0NU16 Trypanosomatidae 36% 67%
A0A1X0NV28 Trypanosomatidae 37% 68%
A0A1X0NWK7 Trypanosomatidae 32% 100%
A0A1X0NY94 Trypanosomatidae 37% 94%
A0A1X0NYE7 Trypanosomatidae 32% 79%
A0A1X0NYN2 Trypanosomatidae 36% 76%
A0A1X0NYS5 Trypanosomatidae 35% 67%
A0A1X0NYZ2 Trypanosomatidae 38% 71%
A0A1X0NZ46 Trypanosomatidae 28% 69%
A0A1X0P055 Trypanosomatidae 37% 68%
A0A1X0P0C4 Trypanosomatidae 32% 78%
A0A1X0P154 Trypanosomatidae 30% 67%
A0A1X0P331 Trypanosomatidae 36% 70%
A0A1X0P3S2 Trypanosomatidae 28% 72%
A0A1X0P4L8 Trypanosomatidae 34% 78%
A0A1X0P5K2 Trypanosomatidae 36% 90%
A0A1X0P7R3 Trypanosomatidae 37% 71%
A0A1X0P8B4 Trypanosomatidae 34% 80%
A0A1X0P9Z4 Trypanosomatidae 34% 73%
A0A1X0PB04 Trypanosomatidae 34% 78%
A0A3Q8I8N3 Leishmania donovani 59% 67%
A0A3Q8I8P8 Leishmania donovani 59% 67%
A0A3Q8I8S9 Leishmania donovani 60% 67%
A0A3Q8IAZ8 Leishmania donovani 59% 67%
A0A3Q8IC35 Leishmania donovani 59% 67%
A0A3Q8IC44 Leishmania donovani 60% 68%
A0A3Q8IH61 Leishmania donovani 59% 68%
A0A3R7JUH9 Trypanosoma rangeli 39% 100%
A0A3R7JUS0 Trypanosoma rangeli 34% 87%
A0A3R7JV87 Trypanosoma rangeli 34% 75%
A0A3R7K7T9 Trypanosoma rangeli 35% 67%
A0A3R7K9S1 Trypanosoma rangeli 35% 77%
A0A3R7KFR4 Trypanosoma rangeli 32% 100%
A0A3R7KJC7 Trypanosoma rangeli 37% 98%
A0A3R7LFZ4 Trypanosoma rangeli 39% 73%
A0A3R7LWG1 Trypanosoma rangeli 35% 71%
A0A3R7LX11 Trypanosoma rangeli 36% 71%
A0A3R7M1R8 Trypanosoma rangeli 35% 70%
A0A3R7MTS2 Trypanosoma rangeli 40% 88%
A0A3R7MW36 Trypanosoma rangeli 35% 75%
A0A3R7N1W7 Trypanosoma rangeli 34% 69%
A0A3R7NTI8 Trypanosoma rangeli 35% 70%
A0A3R7R5R1 Trypanosoma rangeli 36% 67%
A0A3R7R818 Trypanosoma rangeli 32% 76%
A0A3S5H6G0 Leishmania donovani 59% 67%
A0A3S5IQY2 Trypanosoma rangeli 40% 78%
A0A3S7WR43 Leishmania donovani 59% 67%
A0A3S7WR46 Leishmania donovani 59% 67%
A0A3S7WR60 Leishmania donovani 60% 67%
A0A3S7X192 Leishmania donovani 38% 71%
A0A422MQQ6 Trypanosoma rangeli 32% 100%
A0A422MTE4 Trypanosoma rangeli 31% 87%
A0A422MU95 Trypanosoma rangeli 35% 70%
A0A422MUP1 Trypanosoma rangeli 40% 100%
A0A422MVF5 Trypanosoma rangeli 33% 72%
A0A422MVS3 Trypanosoma rangeli 35% 78%
A0A422MXE4 Trypanosoma rangeli 40% 100%
A0A422MZ47 Trypanosoma rangeli 40% 71%
A0A422MZG4 Trypanosoma rangeli 36% 68%
A0A422N361 Trypanosoma rangeli 35% 72%
A0A422NDS2 Trypanosoma rangeli 33% 69%
A0A422NDT3 Trypanosoma rangeli 31% 70%
A0A422NP82 Trypanosoma rangeli 38% 70%
A4H626 Leishmania braziliensis 99% 100%
A4H627 Leishmania braziliensis 72% 100%
A4H629 Leishmania braziliensis 71% 100%
A4H631 Leishmania braziliensis 79% 100%
A4H632 Leishmania braziliensis 68% 100%
A4H633 Leishmania braziliensis 79% 100%
A4H636 Leishmania braziliensis 72% 100%
A4H638 Leishmania braziliensis 67% 68%
A4H6D3 Leishmania braziliensis 75% 100%
A4H6D7 Leishmania braziliensis 71% 100%
A4H6D8 Leishmania braziliensis 81% 100%
A4H6D9 Leishmania braziliensis 81% 100%
A4H6E1 Leishmania braziliensis 75% 100%
A4H6E2 Leishmania braziliensis 75% 100%
A4H6E3 Leishmania braziliensis 81% 70%
A4H6E5 Leishmania braziliensis 68% 78%
A4H6E7 Leishmania braziliensis 70% 100%
A4HJI1 Leishmania braziliensis 37% 100%
A4HUF6 Leishmania infantum 59% 67%
A4HUF8 Leishmania infantum 60% 67%
C9ZUT5 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 36% 69%
D0A1R8 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 31% 68%
D0A7W4 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 34% 90%
E9AHH5 Leishmania infantum 38% 71%
E9AN54 Leishmania mexicana (strain MHOM/GT/2001/U1103) 58% 67%
E9AN57 Leishmania mexicana (strain MHOM/GT/2001/U1103) 58% 67%
E9AZL8 Leishmania mexicana (strain MHOM/GT/2001/U1103) 39% 71%
P08148 Leishmania major 60% 67%
P15706 Leishmania chagasi 60% 67%
P23223 Leishmania donovani 58% 68%
Q27673 Leishmania amazonensis 57% 67%
Q4Q8L3 Leishmania major 40% 100%
Q4QHG9 Leishmania major 59% 67%
Q4QHH1 Leishmania major 60% 67%
Q4QHH2 Leishmania major 60% 67%
Q6LA77 Leishmania infantum 60% 67%
V5A2X8 Trypanosoma cruzi 35% 83%
V5A303 Trypanosoma cruzi 40% 96%
V5A359 Trypanosoma cruzi 40% 74%
V5AII7 Trypanosoma cruzi 40% 74%
V5AJE7 Trypanosoma cruzi 34% 77%
V5AMH8 Trypanosoma cruzi 35% 85%
V5AN34 Trypanosoma cruzi 39% 96%
V5ANT4 Trypanosoma cruzi 40% 100%
V5AZG8 Trypanosoma cruzi 36% 84%
V5B7J8 Trypanosoma cruzi 35% 83%
V5BAB0 Trypanosoma cruzi 36% 100%
V5BAL3 Trypanosoma cruzi 41% 80%
V5BD39 Trypanosoma cruzi 44% 91%
V5CHU5 Trypanosoma cruzi 42% 100%
V5CIA7 Trypanosoma cruzi 34% 84%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2024 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS