Leishmanolysin

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Leishmanolysin

Gene product:

GP63, leishmanolysin (fragment)

Species:

Leishmania braziliensis

UniProt:

A4H6D2_LEIBR

TriTrypDb:

LbrM.10.1560

Length:

340

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 32, no: 19
NetGPI	no	yes: 0, no: 51

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	52
GO:0110165	cellular anatomical entity	1	52

Expansion

Sequence features

A4H6D2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6D2

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	52
GO:0006807	nitrogen compound metabolic process	2	52
GO:0007155	cell adhesion	2	52
GO:0008152	metabolic process	1	52
GO:0009987	cellular process	1	52
GO:0019538	protein metabolic process	3	52
GO:0043170	macromolecule metabolic process	3	52
GO:0044238	primary metabolic process	2	52
GO:0071704	organic substance metabolic process	2	52
GO:1901564	organonitrogen compound metabolic process	3	52

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	52
GO:0004175	endopeptidase activity	4	52
GO:0004222	metalloendopeptidase activity	5	52
GO:0005488	binding	1	52
GO:0008233	peptidase activity	3	52
GO:0008237	metallopeptidase activity	4	52
GO:0016787	hydrolase activity	2	52
GO:0043167	ion binding	2	52
GO:0043169	cation binding	3	52
GO:0046872	metal ion binding	4	52
GO:0140096	catalytic activity, acting on a protein	2	52

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	221	225	PF00656	0.283
CLV_NRD_NRD_1	11	13	PF00675	0.656
CLV_PCSK_KEX2_1	10	12	PF00082	0.671
CLV_PCSK_KEX2_1	141	143	PF00082	0.499
CLV_PCSK_PC1ET2_1	141	143	PF00082	0.505
CLV_PCSK_SKI1_1	327	331	PF00082	0.456
CLV_PCSK_SKI1_1	99	103	PF00082	0.469
DEG_SPOP_SBC_1	82	86	PF00917	0.353
DOC_CYCLIN_RxL_1	96	106	PF00134	0.187
DOC_MAPK_gen_1	184	194	PF00069	0.279
DOC_MAPK_gen_1	279	287	PF00069	0.233
DOC_MAPK_MEF2A_6	187	194	PF00069	0.282
DOC_PP2B_LxvP_1	333	336	PF13499	0.305
DOC_PP2B_LxvP_1	70	73	PF13499	0.347
DOC_PP4_FxxP_1	183	186	PF00568	0.256
DOC_USP7_MATH_1	73	77	PF00917	0.302
DOC_WW_Pin1_4	287	292	PF00397	0.272
DOC_WW_Pin1_4	83	88	PF00397	0.353
LIG_14-3-3_CanoR_1	10	19	PF00244	0.608
LIG_14-3-3_CanoR_1	142	146	PF00244	0.306
LIG_14-3-3_CanoR_1	52	58	PF00244	0.337
LIG_14-3-3_CanoR_1	99	105	PF00244	0.255
LIG_APCC_ABBA_1	154	159	PF00400	0.220
LIG_APCC_ABBA_1	263	268	PF00400	0.341
LIG_BIR_II_1	1	5	PF00653	0.762
LIG_Clathr_ClatBox_1	101	105	PF01394	0.314
LIG_FHA_1	156	162	PF00498	0.230
LIG_FHA_1	174	180	PF00498	0.298
LIG_FHA_1	189	195	PF00498	0.225
LIG_FHA_1	213	219	PF00498	0.287
LIG_FHA_1	95	101	PF00498	0.255
LIG_FHA_2	219	225	PF00498	0.269
LIG_FHA_2	305	311	PF00498	0.270
LIG_GBD_Chelix_1	255	263	PF00786	0.435
LIG_LIR_Apic_2	182	186	PF02991	0.287
LIG_LIR_Apic_2	282	286	PF02991	0.345
LIG_LIR_Gen_1	241	251	PF02991	0.290
LIG_LIR_Gen_1	305	313	PF02991	0.333
LIG_LIR_Nem_3	111	116	PF02991	0.299
LIG_LIR_Nem_3	196	202	PF02991	0.239
LIG_LIR_Nem_3	241	246	PF02991	0.282
LIG_LIR_Nem_3	305	311	PF02991	0.336
LIG_LIR_Nem_3	92	98	PF02991	0.193
LIG_LYPXL_SIV_4	73	81	PF13949	0.147
LIG_NRBOX	97	103	PF00104	0.147
LIG_PCNA_yPIPBox_3	141	150	PF02747	0.290
LIG_PDZ_Class_2	335	340	PF00595	0.343
LIG_SH2_CRK	243	247	PF00017	0.340
LIG_SH2_STAP1	243	247	PF00017	0.340
LIG_SH2_STAT3	116	119	PF00017	0.333
LIG_SH2_STAT5	113	116	PF00017	0.169
LIG_SH2_STAT5	202	205	PF00017	0.246
LIG_SH2_STAT5	238	241	PF00017	0.230
LIG_SH2_STAT5	308	311	PF00017	0.319
LIG_SH2_STAT5	74	77	PF00017	0.226
LIG_SH3_3	204	210	PF00018	0.324
LIG_SUMO_SIM_par_1	100	106	PF11976	0.311
LIG_SxIP_EBH_1	142	156	PF03271	0.305
LIG_TRAF2_1	329	332	PF00917	0.327
LIG_TYR_ITIM	306	311	PF00017	0.338
MOD_CK1_1	205	211	PF00069	0.368
MOD_CK1_1	289	295	PF00069	0.244
MOD_CK2_1	304	310	PF00069	0.388
MOD_GlcNHglycan	240	243	PF01048	0.271
MOD_GlcNHglycan	67	70	PF01048	0.339
MOD_GlcNHglycan	75	78	PF01048	0.316
MOD_GlcNHglycan	91	94	PF01048	0.320
MOD_GSK3_1	141	148	PF00069	0.334
MOD_GSK3_1	166	173	PF00069	0.406
MOD_GSK3_1	188	195	PF00069	0.363
MOD_GSK3_1	311	318	PF00069	0.324
MOD_GSK3_1	81	88	PF00069	0.424
MOD_NEK2_1	145	150	PF00069	0.307
MOD_NEK2_1	247	252	PF00069	0.399
MOD_NEK2_1	311	316	PF00069	0.396
MOD_NEK2_1	81	86	PF00069	0.408
MOD_PKA_1	10	16	PF00069	0.553
MOD_PKA_1	141	147	PF00069	0.428
MOD_PKA_2	141	147	PF00069	0.396
MOD_PKA_2	4	10	PF00069	0.695
MOD_PKB_1	3	11	PF00069	0.604
MOD_Plk_4	112	118	PF00069	0.428
MOD_Plk_4	141	147	PF00069	0.281
MOD_Plk_4	304	310	PF00069	0.405
MOD_ProDKin_1	287	293	PF00069	0.339
MOD_ProDKin_1	83	89	PF00069	0.452
MOD_SUMO_for_1	329	332	PF00179	0.317
TRG_ENDOCYTIC_2	243	246	PF00928	0.380
TRG_ENDOCYTIC_2	308	311	PF00928	0.433
TRG_ER_diArg_1	10	12	PF00400	0.678
TRG_ER_diArg_1	278	281	PF00400	0.338
TRG_NES_CRM1_1	262	274	PF08389	0.357

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P260	Leptomonas seymouri	47%	92%
A0A0N0P2A9	Leptomonas seymouri	50%	93%
A0A0S4IS53	Bodo saltans	34%	71%
A0A1X0NDC6	Trypanosomatidae	26%	91%
A0A1X0NDI6	Trypanosomatidae	31%	100%
A0A1X0NDK0	Trypanosomatidae	26%	99%
A0A1X0NDK9	Trypanosomatidae	33%	70%
A0A1X0NDV4	Trypanosomatidae	28%	100%
A0A1X0NET7	Trypanosomatidae	38%	66%
A0A1X0NFB0	Trypanosomatidae	35%	84%
A0A1X0NFB6	Trypanosomatidae	30%	100%
A0A1X0NFI2	Trypanosomatidae	29%	99%
A0A1X0NFZ9	Trypanosomatidae	32%	74%
A0A1X0NGN3	Trypanosomatidae	37%	82%
A0A1X0NIJ6	Trypanosomatidae	31%	82%
A0A1X0NPU7	Trypanosomatidae	35%	100%
A0A1X0NQM4	Trypanosomatidae	36%	74%
A0A1X0NQN3	Trypanosomatidae	38%	78%
A0A1X0NX09	Trypanosomatidae	33%	100%
A0A1X0NXI9	Trypanosomatidae	37%	100%
A0A1X0NXK4	Trypanosomatidae	26%	93%
A0A1X0NXM9	Trypanosomatidae	34%	100%
A0A1X0NXY0	Trypanosomatidae	27%	99%
A0A1X0NYE7	Trypanosomatidae	34%	67%
A0A1X0NZ76	Trypanosomatidae	27%	97%
A0A1X0NZS0	Trypanosomatidae	27%	86%
A0A1X0P1G3	Trypanosomatidae	28%	92%
A0A1X0P3N6	Trypanosomatidae	33%	69%
A0A1X0P6E8	Trypanosomatidae	34%	93%
A0A1X0P868	Trypanosomatidae	28%	100%
A0A1X0PB04	Trypanosomatidae	35%	67%
A0A3R7K754	Trypanosoma rangeli	38%	91%
A0A3R7KAQ9	Trypanosoma rangeli	37%	100%
A0A3R7L4L7	Trypanosoma rangeli	37%	88%
A0A3R7M5K4	Trypanosoma rangeli	33%	66%
A0A3R7MTS2	Trypanosoma rangeli	38%	75%
A0A3R7N7I3	Trypanosoma rangeli	38%	83%
A0A3R7R4F7	Trypanosoma rangeli	38%	100%
A0A422MNW0	Trypanosoma rangeli	36%	84%
A0A422MP17	Trypanosoma rangeli	40%	76%
A0A422NN26	Trypanosoma rangeli	32%	93%
A0A422NX20	Trypanosoma rangeli	36%	79%
A4H6E5	Leishmania braziliensis	66%	67%
E8NHL1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	65%	100%
V5APB3	Trypanosoma cruzi	37%	100%
V5AUG3	Trypanosoma cruzi	40%	100%
V5B5E7	Trypanosoma cruzi	36%	100%
V5BAL3	Trypanosoma cruzi	39%	67%
V5BD39	Trypanosoma cruzi	39%	77%
V5BIW7	Trypanosoma cruzi	34%	84%