Leishmanolysin

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Leishmanolysin

Gene product:

GP63, leishmanolysin

Species:

Leishmania braziliensis

UniProt:

A4H6D1_LEIBR

TriTrypDb:

LbrM.10.1550 , LBRM2903_310032100

Length:

559

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 5

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 12, no: 4
NetGPI	no	yes: 0, no: 16

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	17
GO:0110165	cellular anatomical entity	1	17

Expansion

Sequence features

A4H6D1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6D1

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	17
GO:0006807	nitrogen compound metabolic process	2	17
GO:0007155	cell adhesion	2	17
GO:0008152	metabolic process	1	17
GO:0009987	cellular process	1	17
GO:0019538	protein metabolic process	3	17
GO:0043170	macromolecule metabolic process	3	17
GO:0044238	primary metabolic process	2	17
GO:0071704	organic substance metabolic process	2	17
GO:1901564	organonitrogen compound metabolic process	3	17

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	17
GO:0004175	endopeptidase activity	4	17
GO:0004222	metalloendopeptidase activity	5	17
GO:0005488	binding	1	17
GO:0008233	peptidase activity	3	17
GO:0008237	metallopeptidase activity	4	17
GO:0016787	hydrolase activity	2	17
GO:0043167	ion binding	2	17
GO:0043169	cation binding	3	17
GO:0046872	metal ion binding	4	17
GO:0140096	catalytic activity, acting on a protein	2	17

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	463	467	PF00656	0.158
CLV_NRD_NRD_1	161	163	PF00675	0.518
CLV_NRD_NRD_1	476	478	PF00675	0.358
CLV_NRD_NRD_1	9	11	PF00675	0.382
CLV_PCSK_KEX2_1	160	162	PF00082	0.537
CLV_PCSK_KEX2_1	291	293	PF00082	0.598
CLV_PCSK_KEX2_1	475	477	PF00082	0.358
CLV_PCSK_KEX2_1	9	11	PF00082	0.382
CLV_PCSK_PC1ET2_1	291	293	PF00082	0.582
CLV_PCSK_PC1ET2_1	475	477	PF00082	0.358
CLV_PCSK_SKI1_1	22	26	PF00082	0.357
CLV_PCSK_SKI1_1	249	253	PF00082	0.405
CLV_PCSK_SKI1_1	399	403	PF00082	0.501
CLV_PCSK_SKI1_1	477	481	PF00082	0.464
CLV_PCSK_SKI1_1	70	74	PF00082	0.368
DEG_Nend_UBRbox_2	1	3	PF02207	0.591
DEG_SPOP_SBC_1	118	122	PF00917	0.560
DEG_SPOP_SBC_1	232	236	PF00917	0.366
DOC_CYCLIN_RxL_1	19	28	PF00134	0.555
DOC_CYCLIN_RxL_1	246	256	PF00134	0.179
DOC_MAPK_gen_1	429	437	PF00069	0.270
DOC_MAPK_MEF2A_6	119	127	PF00069	0.566
DOC_PP1_RVXF_1	506	512	PF00149	0.176
DOC_PP2B_LxvP_1	108	111	PF13499	0.551
DOC_PP2B_LxvP_1	220	223	PF13499	0.366
DOC_PP2B_LxvP_1	483	486	PF13499	0.371
DOC_PP4_FxxP_1	333	336	PF00568	0.392
DOC_USP7_MATH_1	149	153	PF00917	0.660
DOC_USP7_MATH_1	223	227	PF00917	0.344
DOC_WW_Pin1_4	155	160	PF00397	0.604
DOC_WW_Pin1_4	233	238	PF00397	0.366
DOC_WW_Pin1_4	31	36	PF00397	0.567
DOC_WW_Pin1_4	437	442	PF00397	0.323
DOC_WW_Pin1_4	46	51	PF00397	0.585
LIG_14-3-3_CanoR_1	119	124	PF00244	0.584
LIG_14-3-3_CanoR_1	141	151	PF00244	0.633
LIG_14-3-3_CanoR_1	155	159	PF00244	0.728
LIG_14-3-3_CanoR_1	160	169	PF00244	0.672
LIG_14-3-3_CanoR_1	202	208	PF00244	0.360
LIG_14-3-3_CanoR_1	249	255	PF00244	0.387
LIG_14-3-3_CanoR_1	292	296	PF00244	0.244
LIG_14-3-3_CanoR_1	30	34	PF00244	0.568
LIG_APCC_ABBA_1	304	309	PF00400	0.398
LIG_APCC_ABBA_1	413	418	PF00400	0.254
LIG_APCC_ABBA_1	545	550	PF00400	0.355
LIG_Clathr_ClatBox_1	251	255	PF01394	0.174
LIG_Clathr_ClatBox_1	413	417	PF01394	0.163
LIG_CtBP_PxDLS_1	126	130	PF00389	0.569
LIG_FHA_1	118	124	PF00498	0.562
LIG_FHA_1	245	251	PF00498	0.339
LIG_FHA_1	306	312	PF00498	0.267
LIG_FHA_1	324	330	PF00498	0.307
LIG_FHA_1	339	345	PF00498	0.217
LIG_FHA_1	52	58	PF00498	0.595
LIG_FHA_2	455	461	PF00498	0.224
LIG_FHA_2	498	504	PF00498	0.240
LIG_FHA_2	510	516	PF00498	0.225
LIG_LIR_Apic_2	332	336	PF02991	0.398
LIG_LIR_Apic_2	432	436	PF02991	0.328
LIG_LIR_Apic_2	449	454	PF02991	0.224
LIG_LIR_Gen_1	104	113	PF02991	0.556
LIG_LIR_Gen_1	391	401	PF02991	0.379
LIG_LIR_Gen_1	455	463	PF02991	0.394
LIG_LIR_Gen_1	500	509	PF02991	0.163
LIG_LIR_Gen_1	550	559	PF02991	0.360
LIG_LIR_Nem_3	104	108	PF02991	0.560
LIG_LIR_Nem_3	242	248	PF02991	0.319
LIG_LIR_Nem_3	261	266	PF02991	0.228
LIG_LIR_Nem_3	348	352	PF02991	0.360
LIG_LIR_Nem_3	391	396	PF02991	0.379
LIG_LIR_Nem_3	455	461	PF02991	0.289
LIG_LIR_Nem_3	500	504	PF02991	0.218
LIG_LIR_Nem_3	550	554	PF02991	0.359
LIG_LYPXL_SIV_4	223	231	PF13949	0.174
LIG_NRBOX	247	253	PF00104	0.174
LIG_PTAP_UEV_1	98	103	PF05743	0.553
LIG_PTB_Apo_2	343	350	PF02174	0.382
LIG_REV1ctd_RIR_1	370	376	PF16727	0.163
LIG_SH2_CRK	393	397	PF00017	0.243
LIG_SH2_CRK	451	455	PF00017	0.242
LIG_SH2_CRK	493	497	PF00017	0.273
LIG_SH2_STAP1	393	397	PF00017	0.366
LIG_SH2_STAT3	266	269	PF00017	0.344
LIG_SH2_STAT5	113	116	PF00017	0.557
LIG_SH2_STAT5	224	227	PF00017	0.371
LIG_SH2_STAT5	263	266	PF00017	0.296
LIG_SH2_STAT5	352	355	PF00017	0.392
LIG_SH2_STAT5	388	391	PF00017	0.217
LIG_SH2_STAT5	493	496	PF00017	0.262
LIG_SH3_3	120	126	PF00018	0.564
LIG_SH3_3	354	360	PF00018	0.323
LIG_SH3_3	549	555	PF00018	0.380
LIG_SH3_3	90	96	PF00018	0.560
LIG_SUMO_SIM_par_1	125	131	PF11976	0.568
LIG_SUMO_SIM_par_1	250	256	PF11976	0.174
LIG_SxIP_EBH_1	292	306	PF03271	0.174
LIG_TRFH_1	95	99	PF08558	0.434
LIG_WRC_WIRS_1	498	503	PF05994	0.170
LIG_WW_1	110	113	PF00397	0.438
LIG_WW_3	109	113	PF00397	0.433
MOD_CDK_SPK_2	155	160	PF00069	0.507
MOD_CDK_SPxK_1	155	161	PF00069	0.502
MOD_CDK_SPxxK_3	155	162	PF00069	0.499
MOD_CK1_1	128	134	PF00069	0.448
MOD_CK1_1	355	361	PF00069	0.447
MOD_CK1_1	49	55	PF00069	0.494
MOD_CK2_1	454	460	PF00069	0.292
MOD_CK2_1	509	515	PF00069	0.323
MOD_Cter_Amidation	7	10	PF01082	0.484
MOD_DYRK1A_RPxSP_1	155	159	PF00069	0.478
MOD_GlcNHglycan	105	108	PF01048	0.440
MOD_GlcNHglycan	130	133	PF01048	0.448
MOD_GlcNHglycan	217	220	PF01048	0.456
MOD_GlcNHglycan	225	228	PF01048	0.331
MOD_GlcNHglycan	241	244	PF01048	0.198
MOD_GlcNHglycan	39	42	PF01048	0.486
MOD_GlcNHglycan	390	393	PF01048	0.296
MOD_GlcNHglycan	99	102	PF01048	0.428
MOD_GSK3_1	135	142	PF00069	0.485
MOD_GSK3_1	143	150	PF00069	0.497
MOD_GSK3_1	231	238	PF00069	0.305
MOD_GSK3_1	25	32	PF00069	0.440
MOD_GSK3_1	291	298	PF00069	0.185
MOD_GSK3_1	316	323	PF00069	0.498
MOD_GSK3_1	338	345	PF00069	0.493
MOD_GSK3_1	461	468	PF00069	0.247
MOD_GSK3_1	97	104	PF00069	0.455
MOD_NEK2_1	1	6	PF00069	0.481
MOD_NEK2_1	139	144	PF00069	0.537
MOD_NEK2_1	231	236	PF00069	0.289
MOD_NEK2_1	24	29	PF00069	0.436
MOD_NEK2_1	295	300	PF00069	0.185
MOD_NEK2_1	397	402	PF00069	0.435
MOD_PIKK_1	1	7	PF00454	0.483
MOD_PIKK_1	25	31	PF00454	0.439
MOD_PKA_1	160	166	PF00069	0.653
MOD_PKA_1	291	297	PF00069	0.281
MOD_PKA_2	118	124	PF00069	0.478
MOD_PKA_2	142	148	PF00069	0.577
MOD_PKA_2	154	160	PF00069	0.669
MOD_PKA_2	29	35	PF00069	0.452
MOD_PKA_2	291	297	PF00069	0.282
MOD_PKB_1	141	149	PF00069	0.547
MOD_Plk_4	262	268	PF00069	0.371
MOD_Plk_4	291	297	PF00069	0.395
MOD_Plk_4	439	445	PF00069	0.429
MOD_Plk_4	454	460	PF00069	0.239
MOD_ProDKin_1	155	161	PF00069	0.502
MOD_ProDKin_1	233	239	PF00069	0.453
MOD_ProDKin_1	31	37	PF00069	0.457
MOD_ProDKin_1	437	443	PF00069	0.393
MOD_ProDKin_1	46	52	PF00069	0.478
MOD_SUMO_for_1	479	482	PF00179	0.310
TRG_DiLeu_BaEn_1	417	422	PF01217	0.222
TRG_DiLeu_BaEn_4	417	423	PF01217	0.170
TRG_DiLeu_BaLyEn_6	67	72	PF01217	0.450
TRG_ENDOCYTIC_2	393	396	PF00928	0.284
TRG_ENDOCYTIC_2	493	496	PF00928	0.323
TRG_ER_diArg_1	159	162	PF00400	0.680
TRG_ER_diArg_1	428	431	PF00400	0.237
TRG_ER_diArg_1	43	46	PF00400	0.522
TRG_ER_diArg_1	9	12	PF00400	0.474
TRG_NES_CRM1_1	404	418	PF08389	0.302

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IYM3	Bodo saltans	29%	77%
A0A3R7M5K4	Trypanosoma rangeli	33%	100%
A0A3R7MW36	Trypanosoma rangeli	32%	100%
A0A422MRQ6	Trypanosoma rangeli	33%	75%
A4H626	Leishmania braziliensis	79%	97%
A4H627	Leishmania braziliensis	72%	96%
A4H630	Leishmania braziliensis	72%	90%
A4H634	Leishmania braziliensis	74%	91%
A4H635	Leishmania braziliensis	73%	90%
A4H637	Leishmania braziliensis	74%	90%
A4H640	Leishmania braziliensis	74%	90%
A4H6D3	Leishmania braziliensis	77%	96%
A4H6D5	Leishmania braziliensis	73%	79%
A4H6D7	Leishmania braziliensis	71%	96%
A4H6D8	Leishmania braziliensis	80%	96%
A4H6D9	Leishmania braziliensis	80%	96%
A4H6E0	Leishmania braziliensis	82%	79%
A4H6E1	Leishmania braziliensis	76%	96%
A4H6E2	Leishmania braziliensis	76%	97%
P43150	Leishmania mexicana	61%	87%
Q00689	Leishmania guyanensis	72%	90%
Q06031	Crithidia fasciculata	52%	86%
Q27673	Leishmania amazonensis	59%	94%
V5BLT3	Trypanosoma cruzi	32%	89%