Leishmanolysin

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Leishmanolysin

Gene product:

GP63, leishmanolysin

Species:

Leishmania braziliensis

UniProt:

A4H6D0_LEIBR

TriTrypDb:

LbrM.10.1540 , LBRM2903_100014100

Length:

251

Annotations

LeishMANIAdb annotations

Publication identifier(s): 31356625
GPI-anchored cell surface protease. Broad-spectrum ectoenzyme involved in pathogenesis. Heavily expanded family in all parazitic species.. Localization: Cell surface (experimental)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 22
NetGPI	no	yes: 0, no: 22

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	23
GO:0110165	cellular anatomical entity	1	23

Expansion

Sequence features

A4H6D0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H6D0

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	23
GO:0006807	nitrogen compound metabolic process	2	23
GO:0007155	cell adhesion	2	23
GO:0008152	metabolic process	1	23
GO:0009987	cellular process	1	23
GO:0019538	protein metabolic process	3	23
GO:0043170	macromolecule metabolic process	3	23
GO:0044238	primary metabolic process	2	23
GO:0071704	organic substance metabolic process	2	23
GO:1901564	organonitrogen compound metabolic process	3	23

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	23
GO:0004175	endopeptidase activity	4	23
GO:0004222	metalloendopeptidase activity	5	23
GO:0005488	binding	1	23
GO:0008233	peptidase activity	3	23
GO:0008237	metallopeptidase activity	4	23
GO:0016787	hydrolase activity	2	23
GO:0043167	ion binding	2	23
GO:0043169	cation binding	3	23
GO:0046872	metal ion binding	4	23
GO:0140096	catalytic activity, acting on a protein	2	23

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	38	40	PF00675	0.400
CLV_PCSK_KEX2_1	38	40	PF00082	0.398
CLV_Separin_Metazoa	3	7	PF03568	0.125
DEG_SCF_FBW7_1	161	168	PF00400	0.292
DOC_CKS1_1	57	62	PF01111	0.159
DOC_USP7_MATH_1	101	105	PF00917	0.341
DOC_USP7_MATH_1	124	128	PF00917	0.329
DOC_USP7_MATH_1	165	169	PF00917	0.201
DOC_WW_Pin1_4	161	166	PF00397	0.211
DOC_WW_Pin1_4	56	61	PF00397	0.309
DOC_WW_Pin1_4	99	104	PF00397	0.142
LIG_14-3-3_CanoR_1	148	153	PF00244	0.347
LIG_14-3-3_CanoR_1	214	223	PF00244	0.264
LIG_14-3-3_CanoR_1	55	60	PF00244	0.224
LIG_14-3-3_CanoR_1	9	14	PF00244	0.329
LIG_APCC_ABBA_1	108	113	PF00400	0.159
LIG_BIR_II_1	1	5	PF00653	0.185
LIG_BRCT_BRCA1_1	11	15	PF00533	0.338
LIG_BRCT_BRCA1_1	174	178	PF00533	0.275
LIG_DLG_GKlike_1	9	16	PF00625	0.125
LIG_FHA_1	147	153	PF00498	0.261
LIG_FHA_1	225	231	PF00498	0.462
LIG_FHA_1	42	48	PF00498	0.211
LIG_FHA_1	49	55	PF00498	0.184
LIG_FHA_2	162	168	PF00498	0.256
LIG_GBD_Chelix_1	242	250	PF00786	0.336
LIG_IRF3_LxIS_1	242	249	PF10401	0.272
LIG_LIR_Apic_2	37	43	PF02991	0.310
LIG_LIR_Apic_2	56	60	PF02991	0.221
LIG_LIR_Gen_1	12	21	PF02991	0.125
LIG_LIR_Gen_1	63	73	PF02991	0.196
LIG_LIR_Nem_3	12	16	PF02991	0.326
LIG_LIR_Nem_3	63	68	PF02991	0.202
LIG_LIR_Nem_3	77	82	PF02991	0.262
LIG_PCNA_yPIPBox_3	194	205	PF02747	0.194
LIG_Pex14_1	28	32	PF04695	0.150
LIG_Pex14_2	118	122	PF04695	0.125
LIG_Pex14_2	68	72	PF04695	0.159
LIG_PTB_Apo_2	177	184	PF02174	0.142
LIG_PTB_Phospho_1	177	183	PF10480	0.142
LIG_SH2_CRK	13	17	PF00017	0.125
LIG_SH2_CRK	40	44	PF00017	0.283
LIG_SH2_CRK	57	61	PF00017	0.194
LIG_SH2_GRB2like	89	92	PF00017	0.265
LIG_SH2_PTP2	189	192	PF00017	0.159
LIG_SH2_SRC	189	192	PF00017	0.208
LIG_SH2_STAP1	150	154	PF00017	0.175
LIG_SH2_STAT3	150	153	PF00017	0.159
LIG_SH2_STAT5	160	163	PF00017	0.210
LIG_SH2_STAT5	183	186	PF00017	0.321
LIG_SH2_STAT5	189	192	PF00017	0.327
LIG_SH2_STAT5	221	224	PF00017	0.241
LIG_SH2_STAT5	64	67	PF00017	0.194
LIG_SH2_STAT5	82	85	PF00017	0.124
LIG_SH3_3	118	124	PF00018	0.172
LIG_SUMO_SIM_par_1	244	249	PF11976	0.510
MOD_CK1_1	182	188	PF00069	0.301
MOD_CK1_1	99	105	PF00069	0.331
MOD_CK2_1	161	167	PF00069	0.222
MOD_GlcNHglycan	157	160	PF01048	0.486
MOD_GlcNHglycan	174	177	PF01048	0.392
MOD_GlcNHglycan	76	79	PF01048	0.444
MOD_GSK3_1	124	131	PF00069	0.311
MOD_GSK3_1	142	149	PF00069	0.287
MOD_GSK3_1	161	168	PF00069	0.193
MOD_GSK3_1	56	63	PF00069	0.273
MOD_GSK3_1	97	104	PF00069	0.322
MOD_N-GLC_1	179	184	PF02516	0.513
MOD_N-GLC_1	23	28	PF02516	0.347
MOD_N-GLC_1	33	38	PF02516	0.401
MOD_N-GLC_1	9	14	PF02516	0.397
MOD_N-GLC_2	18	20	PF02516	0.451
MOD_NEK2_1	142	147	PF00069	0.159
MOD_NEK2_1	246	251	PF00069	0.552
MOD_PIKK_1	23	29	PF00454	0.253
MOD_PK_1	148	154	PF00069	0.356
MOD_PKA_2	124	130	PF00069	0.241
MOD_PKA_2	154	160	PF00069	0.258
MOD_PKB_1	39	47	PF00069	0.147
MOD_Plk_1	23	29	PF00069	0.272
MOD_Plk_1	9	15	PF00069	0.238
MOD_Plk_4	179	185	PF00069	0.290
MOD_Plk_4	60	66	PF00069	0.321
MOD_ProDKin_1	161	167	PF00069	0.211
MOD_ProDKin_1	56	62	PF00069	0.309
MOD_ProDKin_1	99	105	PF00069	0.142
TRG_ENDOCYTIC_2	13	16	PF00928	0.228
TRG_ENDOCYTIC_2	189	192	PF00928	0.185
TRG_ENDOCYTIC_2	82	85	PF00928	0.222
TRG_ER_diArg_1	38	40	PF00400	0.327
TRG_ER_diArg_1	46	49	PF00400	0.353

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NEJ8	Trypanosomatidae	30%	100%
A0A1X0NGP1	Trypanosomatidae	33%	68%
A0A1X0NGV9	Trypanosomatidae	24%	100%
A0A1X0NHF4	Trypanosomatidae	25%	81%
A0A1X0NKP1	Trypanosomatidae	34%	100%
A0A1X0NP29	Trypanosomatidae	30%	80%
A0A1X0NQ02	Trypanosomatidae	26%	80%
A0A1X0NQU3	Trypanosomatidae	32%	66%
A0A1X0NR02	Trypanosomatidae	30%	74%
A0A1X0NYQ7	Trypanosomatidae	26%	100%
A0A3R7JUH9	Trypanosoma rangeli	31%	73%
A0A3R7LGK6	Trypanosoma rangeli	34%	100%
A0A422MUP1	Trypanosoma rangeli	34%	73%
A0A422MXE4	Trypanosoma rangeli	33%	91%
A0A422MYZ3	Trypanosoma rangeli	33%	100%
A4HJI1	Leishmania braziliensis	34%	85%
V5ANT4	Trypanosoma cruzi	33%	78%
V5AQ95	Trypanosoma cruzi	28%	67%
V5BAB0	Trypanosoma cruzi	28%	71%
V5CIW6	Trypanosoma cruzi	27%	69%
V5CJ71	Trypanosoma cruzi	27%	68%