Thymidylate kinase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

thymidylate kinase-like protein

Species:

Leishmania braziliensis

UniProt:

A4H5Y8_LEIBR

TriTrypDb:

LbrM.10.0060 , LBRM2903_100005500

Length:

363

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H5Y8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H5Y8

Function

Biological processes
Term	Name	Level	Count
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016310	phosphorylation	5	11
GO:0044237	cellular metabolic process	2	11
GO:0006139	nucleobase-containing compound metabolic process	3	5
GO:0006220	pyrimidine nucleotide metabolic process	5	5
GO:0006221	pyrimidine nucleotide biosynthetic process	6	5
GO:0006227	dUDP biosynthetic process	8	1
GO:0006233	dTDP biosynthetic process	8	5
GO:0006235	dTTP biosynthetic process	8	1
GO:0006725	cellular aromatic compound metabolic process	3	5
GO:0006753	nucleoside phosphate metabolic process	4	5
GO:0006807	nitrogen compound metabolic process	2	5
GO:0009058	biosynthetic process	2	5
GO:0009117	nucleotide metabolic process	5	5
GO:0009132	nucleoside diphosphate metabolic process	5	5
GO:0009133	nucleoside diphosphate biosynthetic process	6	5
GO:0009138	pyrimidine nucleoside diphosphate metabolic process	6	5
GO:0009139	pyrimidine nucleoside diphosphate biosynthetic process	7	5
GO:0009141	nucleoside triphosphate metabolic process	5	1
GO:0009142	nucleoside triphosphate biosynthetic process	6	1
GO:0009147	pyrimidine nucleoside triphosphate metabolic process	6	1
GO:0009148	pyrimidine nucleoside triphosphate biosynthetic process	7	1
GO:0009165	nucleotide biosynthetic process	6	5
GO:0009186	deoxyribonucleoside diphosphate metabolic process	6	5
GO:0009189	deoxyribonucleoside diphosphate biosynthetic process	7	5
GO:0009196	pyrimidine deoxyribonucleoside diphosphate metabolic process	7	5
GO:0009197	pyrimidine deoxyribonucleoside diphosphate biosynthetic process	8	5
GO:0009211	pyrimidine deoxyribonucleoside triphosphate metabolic process	7	1
GO:0009212	pyrimidine deoxyribonucleoside triphosphate biosynthetic process	8	1
GO:0009219	pyrimidine deoxyribonucleotide metabolic process	6	5
GO:0009221	pyrimidine deoxyribonucleotide biosynthetic process	7	5
GO:0009262	deoxyribonucleotide metabolic process	4	5
GO:0009263	deoxyribonucleotide biosynthetic process	5	5
GO:0009265	2'-deoxyribonucleotide biosynthetic process	6	5
GO:0009394	2'-deoxyribonucleotide metabolic process	5	5
GO:0018130	heterocycle biosynthetic process	4	5
GO:0019438	aromatic compound biosynthetic process	4	5
GO:0019637	organophosphate metabolic process	3	5
GO:0019692	deoxyribose phosphate metabolic process	4	5
GO:0034641	cellular nitrogen compound metabolic process	3	5
GO:0034654	nucleobase-containing compound biosynthetic process	4	5
GO:0044238	primary metabolic process	2	5
GO:0044249	cellular biosynthetic process	3	5
GO:0044271	cellular nitrogen compound biosynthetic process	4	5
GO:0044281	small molecule metabolic process	2	5
GO:0046072	dTDP metabolic process	7	5
GO:0046075	dTTP metabolic process	7	1
GO:0046077	dUDP metabolic process	7	1
GO:0046385	deoxyribose phosphate biosynthetic process	5	5
GO:0046483	heterocycle metabolic process	3	5
GO:0055086	nucleobase-containing small molecule metabolic process	3	5
GO:0071704	organic substance metabolic process	2	5
GO:0072527	pyrimidine-containing compound metabolic process	4	5
GO:0072528	pyrimidine-containing compound biosynthetic process	5	5
GO:0090407	organophosphate biosynthetic process	4	5
GO:1901135	carbohydrate derivative metabolic process	3	5
GO:1901137	carbohydrate derivative biosynthetic process	4	5
GO:1901293	nucleoside phosphate biosynthetic process	5	5
GO:1901360	organic cyclic compound metabolic process	3	5
GO:1901362	organic cyclic compound biosynthetic process	4	5
GO:1901564	organonitrogen compound metabolic process	3	5
GO:1901566	organonitrogen compound biosynthetic process	4	5
GO:1901576	organic substance biosynthetic process	3	5

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004798	thymidylate kinase activity	6	10
GO:0016301	kinase activity	4	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016776	phosphotransferase activity, phosphate group as acceptor	4	10
GO:0019205	nucleobase-containing compound kinase activity	5	10
GO:0050145	nucleoside monophosphate kinase activity	5	10
GO:0004550	nucleoside diphosphate kinase activity	5	1
GO:0009041	uridylate kinase activity	6	1
GO:0000166	nucleotide binding	3	4
GO:0005488	binding	1	4
GO:0005524	ATP binding	5	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0036094	small molecule binding	2	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097159	organic cyclic compound binding	2	4
GO:0097367	carbohydrate derivative binding	2	4
GO:1901265	nucleoside phosphate binding	3	4
GO:1901363	heterocyclic compound binding	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	144	148	PF00656	0.277
CLV_NRD_NRD_1	120	122	PF00675	0.436
CLV_NRD_NRD_1	224	226	PF00675	0.325
CLV_NRD_NRD_1	289	291	PF00675	0.410
CLV_NRD_NRD_1	293	295	PF00675	0.393
CLV_NRD_NRD_1	336	338	PF00675	0.525
CLV_PCSK_KEX2_1	224	226	PF00082	0.351
CLV_PCSK_KEX2_1	289	291	PF00082	0.397
CLV_PCSK_KEX2_1	309	311	PF00082	0.328
CLV_PCSK_KEX2_1	336	338	PF00082	0.525
CLV_PCSK_PC1ET2_1	309	311	PF00082	0.412
CLV_PCSK_SKI1_1	186	190	PF00082	0.300
CLV_PCSK_SKI1_1	251	255	PF00082	0.416
CLV_PCSK_SKI1_1	290	294	PF00082	0.194
CLV_PCSK_SKI1_1	336	340	PF00082	0.460
DEG_APCC_DBOX_1	60	68	PF00400	0.583
DEG_Nend_UBRbox_1	1	4	PF02207	0.393
DEG_SCF_FBW7_1	98	105	PF00400	0.465
DOC_CKS1_1	99	104	PF01111	0.463
DOC_MAPK_gen_1	224	231	PF00069	0.371
DOC_MAPK_MEF2A_6	270	279	PF00069	0.372
DOC_PP1_RVXF_1	215	222	PF00149	0.419
DOC_PP2B_LxvP_1	277	280	PF13499	0.287
DOC_PP2B_LxvP_1	54	57	PF13499	0.466
DOC_PP4_FxxP_1	266	269	PF00568	0.282
DOC_USP7_MATH_1	133	137	PF00917	0.498
DOC_USP7_MATH_1	22	26	PF00917	0.516
DOC_WW_Pin1_4	104	109	PF00397	0.536
DOC_WW_Pin1_4	131	136	PF00397	0.369
DOC_WW_Pin1_4	98	103	PF00397	0.476
LIG_14-3-3_CanoR_1	224	230	PF00244	0.400
LIG_14-3-3_CanoR_1	270	275	PF00244	0.404
LIG_14-3-3_CanoR_1	310	316	PF00244	0.306
LIG_14-3-3_CanoR_1	349	355	PF00244	0.340
LIG_Actin_WH2_2	27	45	PF00022	0.343
LIG_APCC_ABBAyCdc20_2	2	8	PF00400	0.360
LIG_BRCT_BRCA1_1	355	359	PF00533	0.295
LIG_FHA_1	270	276	PF00498	0.393
LIG_FHA_1	346	352	PF00498	0.518
LIG_FHA_1	95	101	PF00498	0.444
LIG_FHA_2	337	343	PF00498	0.452
LIG_FHA_2	78	84	PF00498	0.537
LIG_GBD_Chelix_1	92	100	PF00786	0.287
LIG_KLC1_Yacidic_2	28	33	PF13176	0.457
LIG_LIR_Apic_2	264	269	PF02991	0.282
LIG_LIR_Gen_1	23	34	PF02991	0.529
LIG_LIR_Gen_1	257	268	PF02991	0.325
LIG_LIR_Gen_1	273	284	PF02991	0.252
LIG_LIR_Gen_1	85	96	PF02991	0.462
LIG_LIR_Nem_3	23	29	PF02991	0.486
LIG_LIR_Nem_3	257	263	PF02991	0.337
LIG_LIR_Nem_3	273	279	PF02991	0.298
LIG_LIR_Nem_3	70	74	PF02991	0.430
LIG_LIR_Nem_3	85	91	PF02991	0.233
LIG_PCNA_yPIPBox_3	193	207	PF02747	0.412
LIG_Pex14_1	46	50	PF04695	0.612
LIG_SH2_CRK	181	185	PF00017	0.337
LIG_SH2_PTP2	31	34	PF00017	0.515
LIG_SH2_SRC	26	29	PF00017	0.533
LIG_SH2_SRC	31	34	PF00017	0.500
LIG_SH2_STAP1	235	239	PF00017	0.371
LIG_SH2_STAP1	26	30	PF00017	0.454
LIG_SH2_STAP1	88	92	PF00017	0.446
LIG_SH2_STAT5	235	238	PF00017	0.278
LIG_SH2_STAT5	276	279	PF00017	0.198
LIG_SH2_STAT5	31	34	PF00017	0.515
LIG_SH2_STAT5	74	77	PF00017	0.473
LIG_SUMO_SIM_anti_2	139	144	PF11976	0.474
LIG_SUMO_SIM_anti_2	94	101	PF11976	0.372
LIG_SUMO_SIM_par_1	139	144	PF11976	0.474
LIG_SUMO_SIM_par_1	96	101	PF11976	0.364
LIG_TRAF2_1	247	250	PF00917	0.306
LIG_TRAF2_1	339	342	PF00917	0.486
LIG_TYR_ITIM	274	279	PF00017	0.287
LIG_TYR_ITIM	29	34	PF00017	0.512
LIG_WRC_WIRS_1	203	208	PF05994	0.405
MOD_CK1_1	117	123	PF00069	0.532
MOD_CK1_1	127	133	PF00069	0.529
MOD_CK1_1	134	140	PF00069	0.494
MOD_CK1_1	353	359	PF00069	0.538
MOD_CK2_1	162	168	PF00069	0.337
MOD_CK2_1	192	198	PF00069	0.397
MOD_CK2_1	22	28	PF00069	0.399
MOD_CK2_1	253	259	PF00069	0.345
MOD_CK2_1	336	342	PF00069	0.512
MOD_Cter_Amidation	190	193	PF01082	0.312
MOD_Cter_Amidation	334	337	PF01082	0.480
MOD_GlcNHglycan	10	13	PF01048	0.551
MOD_GlcNHglycan	102	105	PF01048	0.591
MOD_GlcNHglycan	136	139	PF01048	0.471
MOD_GlcNHglycan	164	167	PF01048	0.195
MOD_GlcNHglycan	189	192	PF01048	0.313
MOD_GSK3_1	127	134	PF00069	0.522
MOD_GSK3_1	202	209	PF00069	0.292
MOD_GSK3_1	37	44	PF00069	0.532
MOD_GSK3_1	4	11	PF00069	0.502
MOD_GSK3_1	73	80	PF00069	0.405
MOD_GSK3_1	94	101	PF00069	0.377
MOD_NEK2_1	184	189	PF00069	0.227
MOD_NEK2_1	206	211	PF00069	0.289
MOD_NEK2_1	67	72	PF00069	0.598
MOD_PIKK_1	150	156	PF00454	0.290
MOD_PK_1	192	198	PF00069	0.397
MOD_PK_1	225	231	PF00069	0.357
MOD_PK_1	270	276	PF00069	0.207
MOD_PKA_1	192	198	PF00069	0.397
MOD_PKA_1	224	230	PF00069	0.419
MOD_PKA_1	336	342	PF00069	0.531
MOD_PKA_2	224	230	PF00069	0.353
MOD_PKA_2	269	275	PF00069	0.491
MOD_PKA_2	311	317	PF00069	0.338
MOD_PKA_2	336	342	PF00069	0.421
MOD_Plk_1	114	120	PF00069	0.608
MOD_Plk_2-3	77	83	PF00069	0.468
MOD_Plk_4	124	130	PF00069	0.521
MOD_Plk_4	225	231	PF00069	0.380
MOD_Plk_4	270	276	PF00069	0.378
MOD_Plk_4	311	317	PF00069	0.193
MOD_Plk_4	67	73	PF00069	0.551
MOD_Plk_4	94	100	PF00069	0.437
MOD_ProDKin_1	131	137	PF00069	0.365
MOD_ProDKin_1	98	104	PF00069	0.491
MOD_SUMO_rev_2	35	45	PF00179	0.377
MOD_SUMO_rev_2	62	70	PF00179	0.634
TRG_DiLeu_BaEn_1	94	99	PF01217	0.545
TRG_DiLeu_BaEn_3	249	255	PF01217	0.306
TRG_ENDOCYTIC_2	181	184	PF00928	0.320
TRG_ENDOCYTIC_2	26	29	PF00928	0.518
TRG_ENDOCYTIC_2	276	279	PF00928	0.272
TRG_ENDOCYTIC_2	31	34	PF00928	0.515
TRG_ENDOCYTIC_2	88	91	PF00928	0.406
TRG_ER_diArg_1	223	225	PF00400	0.371
TRG_ER_diArg_1	288	290	PF00400	0.282
TRG_ER_diArg_1	336	338	PF00400	0.525
TRG_NLS_Bipartite_1	294	313	PF00514	0.182
TRG_NLS_MonoExtC_3	307	312	PF00514	0.182
TRG_NLS_MonoExtN_4	308	313	PF00514	0.182
TRG_Pf-PMV_PEXEL_1	336	341	PF00026	0.532

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HS08	Leptomonas seymouri	62%	100%
A0A0S4KK84	Bodo saltans	34%	96%
A0A1X0NRS1	Trypanosomatidae	46%	100%
A0A3Q8I8L2	Leishmania donovani	85%	100%
A0A3R7KQ58	Trypanosoma rangeli	49%	100%
A4HUB3	Leishmania infantum	85%	100%
C9ZVH7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9AN12	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
Q4QHL4	Leishmania major	85%	100%
V5AU19	Trypanosoma cruzi	48%	100%