Putative translation initiation factor EIF-2B gamma subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

translation initiation factor EIF-2B gamma subunit, putative

Species:

Leishmania braziliensis

UniProt:

A4H5T7_LEIBR

TriTrypDb:

LbrM.09.1140 , LBRM2903_090017900

Length:

602

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 27

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005850	eukaryotic translation initiation factor 2 complex	2	2
GO:0032991	protein-containing complex	1	2
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H5T7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H5T7

Function

Biological processes
Term	Name	Level	Count
GO:0001731	formation of translation preinitiation complex	7	2
GO:0006417	regulation of translation	6	2
GO:0006450	regulation of translational fidelity	3	2
GO:0009889	regulation of biosynthetic process	4	2
GO:0009891	positive regulation of biosynthetic process	5	2
GO:0009893	positive regulation of metabolic process	4	2
GO:0009987	cellular process	1	2
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0010557	positive regulation of macromolecule biosynthetic process	6	2
GO:0010604	positive regulation of macromolecule metabolic process	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0010628	positive regulation of gene expression	6	2
GO:0016043	cellular component organization	3	2
GO:0019222	regulation of metabolic process	3	2
GO:0022607	cellular component assembly	4	2
GO:0022618	ribonucleoprotein complex assembly	6	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031325	positive regulation of cellular metabolic process	5	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0031328	positive regulation of cellular biosynthetic process	6	2
GO:0034248	regulation of amide metabolic process	5	2
GO:0034250	positive regulation of amide metabolic process	6	2
GO:0043933	protein-containing complex organization	4	2
GO:0045727	positive regulation of translation	7	2
GO:0045903	positive regulation of translational fidelity	4	2
GO:0048518	positive regulation of biological process	3	2
GO:0048522	positive regulation of cellular process	4	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051173	positive regulation of nitrogen compound metabolic process	5	2
GO:0051246	regulation of protein metabolic process	5	2
GO:0051247	positive regulation of protein metabolic process	6	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065003	protein-containing complex assembly	5	2
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0071826	ribonucleoprotein complex subunit organization	5	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003676	nucleic acid binding	3	11
GO:0003743	translation initiation factor activity	4	11
GO:0003824	catalytic activity	1	11
GO:0003924	GTPase activity	7	11
GO:0005488	binding	1	11
GO:0005525	GTP binding	5	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0016462	pyrophosphatase activity	5	11
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	11
GO:0019001	guanyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032561	guanyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0000049	tRNA binding	5	6
GO:0003723	RNA binding	4	6
GO:0003746	translation elongation factor activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	114	118	PF00656	0.681
CLV_NRD_NRD_1	251	253	PF00675	0.469
CLV_NRD_NRD_1	312	314	PF00675	0.570
CLV_NRD_NRD_1	319	321	PF00675	0.490
CLV_NRD_NRD_1	393	395	PF00675	0.484
CLV_NRD_NRD_1	519	521	PF00675	0.403
CLV_NRD_NRD_1	577	579	PF00675	0.379
CLV_NRD_NRD_1	588	590	PF00675	0.555
CLV_PCSK_KEX2_1	131	133	PF00082	0.362
CLV_PCSK_KEX2_1	251	253	PF00082	0.481
CLV_PCSK_KEX2_1	311	313	PF00082	0.555
CLV_PCSK_KEX2_1	319	321	PF00082	0.481
CLV_PCSK_KEX2_1	393	395	PF00082	0.484
CLV_PCSK_KEX2_1	519	521	PF00082	0.578
CLV_PCSK_PC1ET2_1	131	133	PF00082	0.362
CLV_PCSK_SKI1_1	126	130	PF00082	0.306
CLV_PCSK_SKI1_1	46	50	PF00082	0.471
CLV_PCSK_SKI1_1	477	481	PF00082	0.417
CLV_PCSK_SKI1_1	569	573	PF00082	0.351
DOC_CDC14_PxL_1	301	309	PF14671	0.442
DOC_CYCLIN_yCln2_LP_2	270	276	PF00134	0.507
DOC_CYCLIN_yCln2_LP_2	449	455	PF00134	0.581
DOC_CYCLIN_yCln2_LP_2	557	563	PF00134	0.403
DOC_MAPK_gen_1	251	262	PF00069	0.466
DOC_MAPK_gen_1	383	391	PF00069	0.442
DOC_MAPK_gen_1	457	465	PF00069	0.483
DOC_MAPK_gen_1	589	596	PF00069	0.416
DOC_MAPK_gen_1	76	85	PF00069	0.562
DOC_MAPK_MEF2A_6	457	465	PF00069	0.496
DOC_MAPK_MEF2A_6	525	534	PF00069	0.351
DOC_MAPK_MEF2A_6	589	596	PF00069	0.416
DOC_MAPK_MEF2A_6	79	87	PF00069	0.562
DOC_MAPK_RevD_3	305	320	PF00069	0.515
DOC_PP1_RVXF_1	579	586	PF00149	0.411
DOC_PP2B_LxvP_1	449	452	PF13499	0.584
DOC_PP2B_PxIxI_1	460	466	PF00149	0.421
DOC_PP4_FxxP_1	169	172	PF00568	0.562
DOC_PP4_FxxP_1	361	364	PF00568	0.456
DOC_USP7_MATH_1	110	114	PF00917	0.492
DOC_USP7_MATH_1	165	169	PF00917	0.681
DOC_USP7_MATH_1	318	322	PF00917	0.411
DOC_USP7_MATH_1	419	423	PF00917	0.695
DOC_USP7_MATH_1	440	444	PF00917	0.586
DOC_USP7_MATH_1	598	602	PF00917	0.552
DOC_WW_Pin1_4	116	121	PF00397	0.589
DOC_WW_Pin1_4	331	336	PF00397	0.617
DOC_WW_Pin1_4	403	408	PF00397	0.687
DOC_WW_Pin1_4	409	414	PF00397	0.690
DOC_WW_Pin1_4	436	441	PF00397	0.633
DOC_WW_Pin1_4	442	447	PF00397	0.583
DOC_WW_Pin1_4	488	493	PF00397	0.614
LIG_14-3-3_CanoR_1	132	137	PF00244	0.562
LIG_14-3-3_CanoR_1	255	263	PF00244	0.511
LIG_14-3-3_CanoR_1	346	350	PF00244	0.492
LIG_14-3-3_CanoR_1	513	518	PF00244	0.403
LIG_Actin_WH2_2	240	257	PF00022	0.530
LIG_Actin_WH2_2	462	479	PF00022	0.424
LIG_Actin_WH2_2	510	527	PF00022	0.286
LIG_Actin_WH2_2	530	547	PF00022	0.351
LIG_APCC_ABBA_1	463	468	PF00400	0.422
LIG_BIR_II_1	1	5	PF00653	0.793
LIG_FHA_1	125	131	PF00498	0.681
LIG_FHA_1	255	261	PF00498	0.382
LIG_FHA_1	345	351	PF00498	0.559
LIG_FHA_1	38	44	PF00498	0.518
LIG_FHA_1	47	53	PF00498	0.418
LIG_FHA_1	84	90	PF00498	0.562
LIG_FHA_2	370	376	PF00498	0.498
LIG_FHA_2	8	14	PF00498	0.796
LIG_Integrin_RGD_1	494	496	PF01839	0.613
LIG_LIR_Apic_2	167	172	PF02991	0.562
LIG_LIR_Apic_2	339	344	PF02991	0.495
LIG_LIR_Apic_2	358	364	PF02991	0.334
LIG_LIR_Gen_1	257	266	PF02991	0.356
LIG_LIR_Gen_1	39	48	PF02991	0.446
LIG_LIR_Nem_3	106	111	PF02991	0.681
LIG_LIR_Nem_3	257	262	PF02991	0.361
LIG_PCNA_PIPBox_1	207	216	PF02747	0.522
LIG_PCNA_PIPBox_1	467	476	PF02747	0.458
LIG_PCNA_yPIPBox_3	460	474	PF02747	0.453
LIG_REV1ctd_RIR_1	471	481	PF16727	0.425
LIG_SH2_CRK	208	212	PF00017	0.565
LIG_SH2_CRK	341	345	PF00017	0.644
LIG_SH2_GRB2like	259	262	PF00017	0.461
LIG_SH2_GRB2like	91	94	PF00017	0.562
LIG_SH2_NCK_1	221	225	PF00017	0.556
LIG_SH2_SRC	259	262	PF00017	0.345
LIG_SH2_SRC	466	469	PF00017	0.403
LIG_SH2_SRC	91	94	PF00017	0.562
LIG_SH2_STAT5	214	217	PF00017	0.681
LIG_SH2_STAT5	243	246	PF00017	0.570
LIG_SH2_STAT5	259	262	PF00017	0.330
LIG_SH2_STAT5	331	334	PF00017	0.588
LIG_SH2_STAT5	575	578	PF00017	0.403
LIG_SH3_3	134	140	PF00018	0.562
LIG_SH3_3	222	228	PF00018	0.562
LIG_SH3_3	242	248	PF00018	0.439
LIG_SH3_3	265	271	PF00018	0.460
LIG_SH3_3	329	335	PF00018	0.583
LIG_Sin3_3	158	165	PF02671	0.562
LIG_SUMO_SIM_anti_2	187	193	PF11976	0.518
LIG_SUMO_SIM_anti_2	299	305	PF11976	0.386
LIG_SUMO_SIM_par_1	366	373	PF11976	0.478
LIG_SUMO_SIM_par_1	528	536	PF11976	0.391
LIG_TRAF2_1	325	328	PF00917	0.461
LIG_TYR_ITSM	255	262	PF00017	0.375
LIG_UBA3_1	189	195	PF00899	0.556
LIG_WRC_WIRS_1	263	268	PF05994	0.489
LIG_WRC_WIRS_1	38	43	PF05994	0.519
MOD_CDK_SPK_2	403	408	PF00069	0.606
MOD_CDK_SPxK_1	488	494	PF00069	0.618
MOD_CK1_1	406	412	PF00069	0.644
MOD_CK1_1	422	428	PF00069	0.649
MOD_CK1_1	434	440	PF00069	0.598
MOD_CK1_1	442	448	PF00069	0.587
MOD_CK1_1	488	494	PF00069	0.594
MOD_CK1_1	533	539	PF00069	0.403
MOD_CK2_1	322	328	PF00069	0.618
MOD_GlcNHglycan	324	327	PF01048	0.550
MOD_GlcNHglycan	399	402	PF01048	0.525
MOD_GlcNHglycan	422	425	PF01048	0.681
MOD_GlcNHglycan	442	445	PF01048	0.645
MOD_GlcNHglycan	561	564	PF01048	0.403
MOD_GlcNHglycan	69	72	PF01048	0.362
MOD_GSK3_1	111	118	PF00069	0.559
MOD_GSK3_1	262	269	PF00069	0.476
MOD_GSK3_1	318	325	PF00069	0.416
MOD_GSK3_1	370	377	PF00069	0.670
MOD_GSK3_1	42	49	PF00069	0.475
MOD_GSK3_1	420	427	PF00069	0.682
MOD_GSK3_1	430	437	PF00069	0.609
MOD_GSK3_1	526	533	PF00069	0.440
MOD_GSK3_1	535	542	PF00069	0.390
MOD_GSK3_1	67	74	PF00069	0.562
MOD_N-GLC_1	165	170	PF02516	0.481
MOD_N-GLC_1	235	240	PF02516	0.356
MOD_N-GLC_1	434	439	PF02516	0.589
MOD_N-GLC_1	488	493	PF02516	0.591
MOD_N-GLC_1	83	88	PF02516	0.362
MOD_NEK2_1	145	150	PF00069	0.562
MOD_NEK2_1	254	259	PF00069	0.477
MOD_NEK2_1	266	271	PF00069	0.456
MOD_NEK2_1	345	350	PF00069	0.453
MOD_NEK2_1	428	433	PF00069	0.581
MOD_NEK2_1	544	549	PF00069	0.403
MOD_NEK2_1	83	88	PF00069	0.562
MOD_NEK2_2	584	589	PF00069	0.461
MOD_OFUCOSY	102	107	PF10250	0.481
MOD_PIKK_1	111	117	PF00454	0.562
MOD_PIKK_1	254	260	PF00454	0.382
MOD_PIKK_1	71	77	PF00454	0.562
MOD_PK_1	513	519	PF00069	0.286
MOD_PKA_2	103	109	PF00069	0.681
MOD_PKA_2	254	260	PF00069	0.522
MOD_PKA_2	318	324	PF00069	0.521
MOD_PKA_2	345	351	PF00069	0.490
MOD_PKA_2	485	491	PF00069	0.627
MOD_PKA_2	512	518	PF00069	0.286
MOD_PKA_2	544	550	PF00069	0.403
MOD_Plk_1	165	171	PF00069	0.681
MOD_Plk_1	235	241	PF00069	0.556
MOD_Plk_1	266	272	PF00069	0.475
MOD_Plk_1	7	13	PF00069	0.795
MOD_Plk_1	83	89	PF00069	0.562
MOD_Plk_4	145	151	PF00069	0.562
MOD_Plk_4	185	191	PF00069	0.519
MOD_Plk_4	235	241	PF00069	0.473
MOD_Plk_4	345	351	PF00069	0.624
MOD_Plk_4	526	532	PF00069	0.376
MOD_Plk_4	539	545	PF00069	0.468
MOD_Plk_4	61	67	PF00069	0.562
MOD_ProDKin_1	116	122	PF00069	0.589
MOD_ProDKin_1	331	337	PF00069	0.622
MOD_ProDKin_1	403	409	PF00069	0.686
MOD_ProDKin_1	436	442	PF00069	0.634
MOD_ProDKin_1	488	494	PF00069	0.618
TRG_ENDOCYTIC_2	208	211	PF00928	0.656
TRG_ENDOCYTIC_2	259	262	PF00928	0.461
TRG_ENDOCYTIC_2	466	469	PF00928	0.462
TRG_ER_diArg_1	250	252	PF00400	0.454
TRG_ER_diArg_1	310	313	PF00400	0.697
TRG_ER_diArg_1	318	320	PF00400	0.551
TRG_ER_diArg_1	392	394	PF00400	0.509
TRG_ER_diArg_1	519	521	PF00400	0.403
TRG_NES_CRM1_1	182	193	PF08389	0.681
TRG_Pf-PMV_PEXEL_1	595	599	PF00026	0.570

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NJL3	Trypanosomatidae	55%	100%
A0A3S7WQT0	Leishmania donovani	69%	100%
A4H5T6	Leishmania braziliensis	70%	100%
A4HU29	Leishmania infantum	69%	100%
A4HU30	Leishmania infantum	85%	100%
E9AMV9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	100%
E9AMW0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
Q4QHR6	Leishmania major	86%	100%
Q4QHR7	Leishmania major	69%	100%