Putative cleavage and polyadenylation specificity factor 30 kDa subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cleavage and polyadenylation specificity factor 30 kDa subunit, putative

Species:

Leishmania braziliensis

UniProt:

A4H5Q3_LEIBR

TriTrypDb:

LbrM.09.0800 , LBRM2903_090013200

Length:

354

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005847	mRNA cleavage and polyadenylation specificity factor complex	4	1
GO:0005849	mRNA cleavage factor complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A4H5Q3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H5Q3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006379	mRNA cleavage	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	12
GO:0090501	RNA phosphodiester bond hydrolysis	6	12
GO:0098787	mRNA cleavage involved in mRNA processing	8	12
GO:0098789	pre-mRNA cleavage required for polyadenylation	9	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006396	RNA processing	6	1
GO:0006397	mRNA processing	7	1
GO:0031426	polycistronic mRNA processing	8	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0005488	binding	1	12
GO:0008270	zinc ion binding	6	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0046914	transition metal ion binding	5	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	122	124	PF00675	0.366
CLV_NRD_NRD_1	249	251	PF00675	0.264
CLV_NRD_NRD_1	297	299	PF00675	0.607
CLV_NRD_NRD_1	300	302	PF00675	0.603
CLV_NRD_NRD_1	347	349	PF00675	0.710
CLV_PCSK_FUR_1	298	302	PF00082	0.705
CLV_PCSK_KEX2_1	122	124	PF00082	0.374
CLV_PCSK_KEX2_1	138	140	PF00082	0.395
CLV_PCSK_KEX2_1	174	176	PF00082	0.529
CLV_PCSK_KEX2_1	249	251	PF00082	0.388
CLV_PCSK_KEX2_1	296	298	PF00082	0.611
CLV_PCSK_KEX2_1	300	302	PF00082	0.601
CLV_PCSK_KEX2_1	346	348	PF00082	0.719
CLV_PCSK_PC1ET2_1	138	140	PF00082	0.520
CLV_PCSK_PC1ET2_1	174	176	PF00082	0.522
CLV_PCSK_PC7_1	296	302	PF00082	0.708
CLV_PCSK_SKI1_1	135	139	PF00082	0.368
CLV_PCSK_SKI1_1	174	178	PF00082	0.537
CLV_PCSK_SKI1_1	192	196	PF00082	0.363
CLV_PCSK_SKI1_1	250	254	PF00082	0.385
DEG_Nend_Nbox_1	1	3	PF02207	0.470
DOC_MAPK_gen_1	174	182	PF00069	0.382
DOC_USP7_MATH_1	111	115	PF00917	0.555
DOC_USP7_MATH_1	214	218	PF00917	0.562
DOC_USP7_MATH_1	235	239	PF00917	0.524
DOC_USP7_MATH_1	284	288	PF00917	0.712
LIG_14-3-3_CanoR_1	48	57	PF00244	0.491
LIG_Actin_WH2_2	54	69	PF00022	0.340
LIG_BIR_III_2	43	47	PF00653	0.507
LIG_BIR_III_4	5	9	PF00653	0.549
LIG_FHA_1	231	237	PF00498	0.704
LIG_FHA_2	258	264	PF00498	0.368
LIG_FHA_2	7	13	PF00498	0.554
LIG_FHA_2	98	104	PF00498	0.415
LIG_LIR_Nem_3	52	57	PF02991	0.524
LIG_PCNA_PIPBox_1	58	67	PF02747	0.335
LIG_REV1ctd_RIR_1	90	99	PF16727	0.507
LIG_SH2_CRK	54	58	PF00017	0.521
LIG_SH2_GRB2like	82	85	PF00017	0.480
LIG_SH2_STAP1	167	171	PF00017	0.461
LIG_SH2_STAP1	82	86	PF00017	0.335
LIG_SH2_STAT3	86	89	PF00017	0.454
LIG_SH2_STAT5	146	149	PF00017	0.429
LIG_SH2_STAT5	78	81	PF00017	0.241
LIG_SH2_STAT5	86	89	PF00017	0.311
LIG_SH3_3	124	130	PF00018	0.350
LIG_SH3_3	149	155	PF00018	0.467
LIG_SH3_3	206	212	PF00018	0.420
LIG_SH3_3	226	232	PF00018	0.334
LIG_SH3_3	278	284	PF00018	0.772
LIG_SH3_3	99	105	PF00018	0.404
LIG_TRAF2_1	162	165	PF00917	0.443
MOD_CK1_1	287	293	PF00069	0.627
MOD_CK2_1	111	117	PF00069	0.426
MOD_CK2_1	97	103	PF00069	0.433
MOD_Cter_Amidation	344	347	PF01082	0.639
MOD_GlcNHglycan	140	143	PF01048	0.556
MOD_GlcNHglycan	192	195	PF01048	0.489
MOD_GlcNHglycan	277	280	PF01048	0.723
MOD_GlcNHglycan	286	289	PF01048	0.716
MOD_GlcNHglycan	51	54	PF01048	0.461
MOD_GSK3_1	186	193	PF00069	0.533
MOD_GSK3_1	49	56	PF00069	0.388
MOD_LATS_1	136	142	PF00433	0.494
MOD_N-GLC_1	111	116	PF02516	0.473
MOD_N-GLC_1	190	195	PF02516	0.378
MOD_NEK2_1	106	111	PF00069	0.594
MOD_NEK2_1	13	18	PF00069	0.511
MOD_NEK2_1	190	195	PF00069	0.499
MOD_NEK2_1	236	241	PF00069	0.662
MOD_PIKK_1	111	117	PF00454	0.462
MOD_PKA_1	138	144	PF00069	0.490
MOD_PKA_2	138	144	PF00069	0.442
MOD_Plk_1	13	19	PF00069	0.516
MOD_Plk_2-3	257	263	PF00069	0.384
MOD_Plk_4	214	220	PF00069	0.546
MOD_SUMO_for_1	137	140	PF00179	0.497
MOD_SUMO_rev_2	20	28	PF00179	0.597
TRG_ENDOCYTIC_2	54	57	PF00928	0.528
TRG_ER_diArg_1	121	123	PF00400	0.355
TRG_ER_diArg_1	296	298	PF00400	0.700
TRG_ER_diArg_1	299	301	PF00400	0.695
TRG_ER_diArg_1	346	348	PF00400	0.721
TRG_ER_diArg_1	38	41	PF00400	0.507
TRG_Pf-PMV_PEXEL_1	221	225	PF00026	0.567
TRG_Pf-PMV_PEXEL_1	297	302	PF00026	0.712

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEE4	Leptomonas seymouri	88%	100%
A0A0S4KP33	Bodo saltans	50%	100%
A0A1X0NMX4	Trypanosomatidae	60%	100%
A0A3Q8IAT5	Leishmania donovani	97%	100%
A0A3R7KCZ6	Trypanosoma rangeli	59%	100%
A4HTZ3	Leishmania infantum	97%	100%
D0A9F4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9AMS3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O95639	Homo sapiens	33%	100%
Q4QHV2	Leishmania major	88%	95%
V5D8L4	Trypanosoma cruzi	63%	100%