tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Methyltransferase TYW3, putative

Species:

Leishmania braziliensis

UniProt:

A4H5P7_LEIBR

TriTrypDb:

LbrM.09.0740 , LBRM2903_090012300

Length:

325

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H5P7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H5P7

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006396	RNA processing	6	13
GO:0006399	tRNA metabolic process	7	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008033	tRNA processing	8	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0032259	methylation	2	13
GO:0034470	ncRNA processing	7	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0034660	ncRNA metabolic process	6	13
GO:0043170	macromolecule metabolic process	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13
GO:0001510	RNA methylation	4	1
GO:0006400	tRNA modification	6	1
GO:0009451	RNA modification	5	1
GO:0030488	tRNA methylation	5	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0008168	methyltransferase activity	4	13
GO:0016740	transferase activity	2	13
GO:0016741	transferase activity, transferring one-carbon groups	3	13
GO:0008173	RNA methyltransferase activity	4	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	4	6	PF00675	0.722
CLV_PCSK_FUR_1	112	116	PF00082	0.450
CLV_PCSK_KEX2_1	114	116	PF00082	0.323
CLV_PCSK_PC1ET2_1	114	116	PF00082	0.323
CLV_PCSK_SKI1_1	105	109	PF00082	0.315
CLV_PCSK_SKI1_1	118	122	PF00082	0.193
CLV_PCSK_SKI1_1	238	242	PF00082	0.298
CLV_PCSK_SKI1_1	87	91	PF00082	0.328
DEG_SCF_TRCP1_1	247	253	PF00400	0.418
DEG_SPOP_SBC_1	79	83	PF00917	0.370
DOC_CYCLIN_RxL_1	232	242	PF00134	0.432
DOC_CYCLIN_yCln2_LP_2	274	280	PF00134	0.336
DOC_MAPK_DCC_7	127	136	PF00069	0.219
DOC_MAPK_MEF2A_6	215	222	PF00069	0.419
DOC_MAPK_MEF2A_6	267	276	PF00069	0.352
DOC_PP2B_LxvP_1	210	213	PF13499	0.450
DOC_PP2B_LxvP_1	274	277	PF13499	0.336
DOC_PP4_FxxP_1	19	22	PF00568	0.497
DOC_USP7_MATH_1	168	172	PF00917	0.443
DOC_USP7_MATH_1	200	204	PF00917	0.469
DOC_USP7_MATH_1	243	247	PF00917	0.385
DOC_USP7_MATH_1	57	61	PF00917	0.440
DOC_USP7_UBL2_3	110	114	PF12436	0.312
DOC_USP7_UBL2_3	2	6	PF12436	0.718
DOC_USP7_UBL2_3	31	35	PF12436	0.531
DOC_WW_Pin1_4	105	110	PF00397	0.454
DOC_WW_Pin1_4	146	151	PF00397	0.395
DOC_WW_Pin1_4	180	185	PF00397	0.252
DOC_WW_Pin1_4	53	58	PF00397	0.336
DOC_WW_Pin1_4	63	68	PF00397	0.219
LIG_14-3-3_CanoR_1	267	274	PF00244	0.341
LIG_APCC_ABBA_1	225	230	PF00400	0.306
LIG_CtBP_PxDLS_1	133	137	PF00389	0.472
LIG_FHA_1	215	221	PF00498	0.390
LIG_FHA_1	267	273	PF00498	0.295
LIG_FHA_1	40	46	PF00498	0.397
LIG_FHA_2	146	152	PF00498	0.399
LIG_FHA_2	72	78	PF00498	0.373
LIG_FHA_2	93	99	PF00498	0.337
LIG_Integrin_RGD_1	115	117	PF01839	0.365
LIG_PCNA_PIPBox_1	136	145	PF02747	0.450
LIG_PDZ_Class_2	320	325	PF00595	0.588
LIG_SH2_STAT5	292	295	PF00017	0.359
LIG_SH2_STAT5	78	81	PF00017	0.328
LIG_SH3_3	252	258	PF00018	0.336
LIG_SH3_4	110	117	PF00018	0.316
LIG_SH3_4	2	9	PF00018	0.763
LIG_Sin3_3	237	244	PF02671	0.396
LIG_UBA3_1	296	303	PF00899	0.336
MOD_CDK_SPK_2	105	110	PF00069	0.445
MOD_CDK_SPK_2	53	58	PF00069	0.336
MOD_CDK_SPxxK_3	105	112	PF00069	0.454
MOD_CK1_1	176	182	PF00069	0.244
MOD_CK1_1	82	88	PF00069	0.326
MOD_CK2_1	145	151	PF00069	0.424
MOD_CK2_1	200	206	PF00069	0.469
MOD_CK2_1	92	98	PF00069	0.365
MOD_Cter_Amidation	258	261	PF01082	0.352
MOD_GlcNHglycan	170	173	PF01048	0.443
MOD_GlcNHglycan	202	205	PF01048	0.433
MOD_GlcNHglycan	23	28	PF01048	0.714
MOD_GlcNHglycan	241	244	PF01048	0.348
MOD_GlcNHglycan	247	250	PF01048	0.347
MOD_GlcNHglycan	252	255	PF01048	0.363
MOD_GlcNHglycan	84	87	PF01048	0.323
MOD_GSK3_1	173	180	PF00069	0.459
MOD_GSK3_1	239	246	PF00069	0.344
MOD_GSK3_1	53	60	PF00069	0.426
MOD_GSK3_1	78	85	PF00069	0.344
MOD_GSK3_1	99	106	PF00069	0.370
MOD_N-GLC_1	92	97	PF02516	0.433
MOD_N-GLC_2	51	53	PF02516	0.352
MOD_NEK2_1	239	244	PF00069	0.393
MOD_NEK2_1	71	76	PF00069	0.378
MOD_NEK2_2	216	221	PF00069	0.370
MOD_PIKK_1	285	291	PF00454	0.465
MOD_PKA_1	6	12	PF00069	0.672
MOD_PKA_2	214	220	PF00069	0.409
MOD_PKA_2	250	256	PF00069	0.328
MOD_PKA_2	266	272	PF00069	0.315
MOD_Plk_2-3	230	236	PF00069	0.306
MOD_ProDKin_1	105	111	PF00069	0.454
MOD_ProDKin_1	146	152	PF00069	0.395
MOD_ProDKin_1	180	186	PF00069	0.252
MOD_ProDKin_1	53	59	PF00069	0.336
MOD_ProDKin_1	63	69	PF00069	0.219
MOD_SUMO_for_1	220	223	PF00179	0.323
MOD_SUMO_rev_2	302	311	PF00179	0.404
MOD_SUMO_rev_2	56	66	PF00179	0.370
MOD_SUMO_rev_2	9	16	PF00179	0.553
MOD_SUMO_rev_2	97	107	PF00179	0.219
TRG_DiLeu_BaEn_1	235	240	PF01217	0.472
TRG_DiLeu_BaLyEn_6	309	314	PF01217	0.374
TRG_NLS_MonoExtN_4	2	9	PF00514	0.744

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P754	Leptomonas seymouri	64%	94%
A0A0S4JMK0	Bodo saltans	40%	100%
A0A1X0NNC3	Trypanosomatidae	51%	94%
A0A1X0NRX8	Trypanosomatidae	28%	100%
A0A3Q8IBW6	Leishmania donovani	82%	92%
A0A3S5IRY7	Trypanosoma rangeli	49%	100%
A4HTY7	Leishmania infantum	82%	92%
D0A9E3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	91%
E9AMR7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	94%
Q4QHV8	Leishmania major	79%	100%
V5DJH9	Trypanosoma cruzi	47%	84%