E3 ubiquitin-protein ligase CHFR

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

FHA domain/Ring finger domain containing protein, putative

Species:

Leishmania braziliensis

UniProt:

A4H5J1_LEIBR

TriTrypDb:

LbrM.09.0160 , LBRM2903_090006500

Length:

521

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016604	nuclear body	2	12
GO:0016605	PML body	3	12
GO:0110165	cellular anatomical entity	1	12
GO:0005634	nucleus	5	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A4H5J1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H5J1

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0016567	protein ubiquitination	7	12
GO:0019538	protein metabolic process	3	12
GO:0032446	protein modification by small protein conjugation	6	12
GO:0036211	protein modification process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044238	primary metabolic process	2	12
GO:0070647	protein modification by small protein conjugation or removal	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0003824	catalytic activity	1	1
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	10	12	PF00675	0.460
CLV_NRD_NRD_1	116	118	PF00675	0.346
CLV_NRD_NRD_1	250	252	PF00675	0.588
CLV_NRD_NRD_1	419	421	PF00675	0.225
CLV_NRD_NRD_1	473	475	PF00675	0.249
CLV_NRD_NRD_1	518	520	PF00675	0.484
CLV_PCSK_KEX2_1	116	118	PF00082	0.398
CLV_PCSK_KEX2_1	250	252	PF00082	0.498
CLV_PCSK_KEX2_1	274	276	PF00082	0.578
CLV_PCSK_KEX2_1	419	421	PF00082	0.169
CLV_PCSK_KEX2_1	473	475	PF00082	0.244
CLV_PCSK_KEX2_1	518	520	PF00082	0.476
CLV_PCSK_PC1ET2_1	274	276	PF00082	0.543
CLV_PCSK_PC1ET2_1	518	520	PF00082	0.476
CLV_PCSK_SKI1_1	117	121	PF00082	0.343
CLV_PCSK_SKI1_1	123	127	PF00082	0.291
CLV_PCSK_SKI1_1	161	165	PF00082	0.423
CLV_PCSK_SKI1_1	186	190	PF00082	0.300
CLV_PCSK_SKI1_1	419	423	PF00082	0.281
CLV_PCSK_SKI1_1	467	471	PF00082	0.410
DEG_APCC_DBOX_1	419	427	PF00400	0.169
DEG_APCC_DBOX_1	99	107	PF00400	0.394
DOC_CYCLIN_RxL_1	417	425	PF00134	0.169
DOC_CYCLIN_RxL_1	464	471	PF00134	0.255
DOC_CYCLIN_yCln2_LP_2	38	44	PF00134	0.538
DOC_MAPK_gen_1	116	126	PF00069	0.255
DOC_MAPK_RevD_3	101	117	PF00069	0.386
DOC_PP1_RVXF_1	465	471	PF00149	0.169
DOC_PP2B_LxvP_1	33	36	PF13499	0.581
DOC_PP4_FxxP_1	189	192	PF00568	0.345
DOC_PP4_FxxP_1	331	334	PF00568	0.311
DOC_USP7_MATH_1	137	141	PF00917	0.554
DOC_USP7_MATH_1	174	178	PF00917	0.608
DOC_USP7_MATH_1	317	321	PF00917	0.232
DOC_WW_Pin1_4	11	16	PF00397	0.636
DOC_WW_Pin1_4	3	8	PF00397	0.682
DOC_WW_Pin1_4	321	326	PF00397	0.394
DOC_WW_Pin1_4	451	456	PF00397	0.345
LIG_14-3-3_CanoR_1	191	196	PF00244	0.286
LIG_14-3-3_CanoR_1	250	259	PF00244	0.482
LIG_14-3-3_CanoR_1	341	349	PF00244	0.394
LIG_14-3-3_CanoR_1	459	465	PF00244	0.460
LIG_APCC_ABBA_1	385	390	PF00400	0.332
LIG_BIR_II_1	1	5	PF00653	0.623
LIG_EVH1_2	398	402	PF00568	0.345
LIG_FHA_1	133	139	PF00498	0.343
LIG_FHA_1	179	185	PF00498	0.280
LIG_FHA_1	230	236	PF00498	0.397
LIG_FHA_1	262	268	PF00498	0.398
LIG_FHA_1	416	422	PF00498	0.209
LIG_FHA_1	53	59	PF00498	0.443
LIG_FHA_1	96	102	PF00498	0.315
LIG_FHA_2	216	222	PF00498	0.169
LIG_FHA_2	250	256	PF00498	0.376
LIG_FHA_2	430	436	PF00498	0.169
LIG_Integrin_isoDGR_2	114	116	PF01839	0.307
LIG_LIR_Apic_2	187	192	PF02991	0.397
LIG_LIR_Apic_2	329	334	PF02991	0.267
LIG_LIR_Gen_1	463	472	PF02991	0.259
LIG_LIR_Nem_3	463	468	PF02991	0.314
LIG_PCNA_PIPBox_1	409	418	PF02747	0.251
LIG_PCNA_yPIPBox_3	409	420	PF02747	0.183
LIG_PCNA_yPIPBox_3	451	465	PF02747	0.365
LIG_PTB_Apo_2	362	369	PF02174	0.218
LIG_SH2_STAT5	415	418	PF00017	0.251
LIG_SH3_2	384	389	PF14604	0.281
LIG_SH3_3	381	387	PF00018	0.375
LIG_SH3_3	393	399	PF00018	0.443
LIG_TRAF2_1	252	255	PF00917	0.484
LIG_UBA3_1	163	168	PF00899	0.466
MOD_CDK_SPxxK_3	11	18	PF00069	0.607
MOD_CK1_1	249	255	PF00069	0.483
MOD_CK1_1	268	274	PF00069	0.606
MOD_CK2_1	215	221	PF00069	0.271
MOD_CK2_1	249	255	PF00069	0.386
MOD_CK2_1	429	435	PF00069	0.295
MOD_Cter_Amidation	114	117	PF01082	0.348
MOD_Cter_Amidation	272	275	PF01082	0.635
MOD_GlcNHglycan	139	142	PF01048	0.590
MOD_GlcNHglycan	267	270	PF01048	0.499
MOD_GlcNHglycan	342	345	PF01048	0.332
MOD_GlcNHglycan	427	430	PF01048	0.375
MOD_GlcNHglycan	61	64	PF01048	0.480
MOD_GlcNHglycan	85	88	PF01048	0.293
MOD_GSK3_1	104	111	PF00069	0.287
MOD_GSK3_1	174	181	PF00069	0.550
MOD_GSK3_1	242	249	PF00069	0.419
MOD_GSK3_1	261	268	PF00069	0.514
MOD_GSK3_1	317	324	PF00069	0.373
MOD_GSK3_1	411	418	PF00069	0.233
MOD_GSK3_1	425	432	PF00069	0.313
MOD_GSK3_1	479	486	PF00069	0.551
MOD_GSK3_1	79	86	PF00069	0.451
MOD_N-GLC_1	108	113	PF02516	0.251
MOD_N-GLC_1	224	229	PF02516	0.280
MOD_NEK2_1	180	185	PF00069	0.300
MOD_NEK2_1	340	345	PF00069	0.354
MOD_NEK2_1	481	486	PF00069	0.448
MOD_NEK2_2	460	465	PF00069	0.345
MOD_PIKK_1	169	175	PF00454	0.449
MOD_PK_1	191	197	PF00069	0.345
MOD_PK_1	392	398	PF00069	0.169
MOD_PKA_1	250	256	PF00069	0.307
MOD_PKA_2	174	180	PF00069	0.464
MOD_PKA_2	249	255	PF00069	0.321
MOD_PKA_2	340	346	PF00069	0.332
MOD_Plk_1	22	28	PF00069	0.425
MOD_Plk_4	108	114	PF00069	0.251
MOD_Plk_4	191	197	PF00069	0.268
MOD_Plk_4	392	398	PF00069	0.258
MOD_Plk_4	411	417	PF00069	0.239
MOD_Plk_4	460	466	PF00069	0.307
MOD_ProDKin_1	11	17	PF00069	0.636
MOD_ProDKin_1	3	9	PF00069	0.682
MOD_ProDKin_1	321	327	PF00069	0.394
MOD_ProDKin_1	451	457	PF00069	0.345
MOD_SUMO_for_1	119	122	PF00179	0.386
MOD_SUMO_for_1	167	170	PF00179	0.484
TRG_DiLeu_BaEn_4	254	260	PF01217	0.452
TRG_DiLeu_BaLyEn_6	464	469	PF01217	0.169
TRG_ER_diArg_1	116	118	PF00400	0.307
TRG_ER_diArg_1	419	421	PF00400	0.390
TRG_ER_diArg_1	472	474	PF00400	0.304
TRG_NES_CRM1_1	91	105	PF08389	0.281
TRG_NLS_MonoExtC_3	273	279	PF00514	0.544
TRG_Pf-PMV_PEXEL_1	420	424	PF00026	0.169
TRG_Pf-PMV_PEXEL_1	467	471	PF00026	0.315

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P377	Leptomonas seymouri	63%	97%
A0A0S4JT63	Bodo saltans	40%	100%
A0A1X0NPC5	Trypanosomatidae	43%	98%
A0A3R7MDE3	Trypanosoma rangeli	42%	95%
A0A3S5H6B1	Leishmania donovani	75%	99%
A4HTT2	Leishmania infantum	75%	99%
A5WW08	Danio rerio	31%	82%
D0A993	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	94%
E9AML5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	75%	99%
Q4QI21	Leishmania major	75%	100%
Q5FWP4	Xenopus laevis	29%	83%
Q5RF77	Pongo abelii	26%	91%
Q6P256	Xenopus tropicalis	29%	83%
Q810L3	Mus musculus	28%	78%
Q96EP1	Homo sapiens	27%	78%
V5DJC2	Trypanosoma cruzi	42%	83%