EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function (DUF2453), putative

Species:

Leishmania braziliensis

UniProt:

A4H5E2_LEIBR

TriTrypDb:

LbrM.08.0790 , LBRM2903_080015400

Length:

839

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	6
GO:0110165	cellular anatomical entity	1	6
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A4H5E2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H5E2

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	6
GO:0005509	calcium ion binding	5	6
GO:0043167	ion binding	2	6
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	187	191	PF00656	0.514
CLV_C14_Caspase3-7	257	261	PF00656	0.469
CLV_C14_Caspase3-7	447	451	PF00656	0.472
CLV_C14_Caspase3-7	517	521	PF00656	0.491
CLV_NRD_NRD_1	2	4	PF00675	0.495
CLV_NRD_NRD_1	32	34	PF00675	0.703
CLV_NRD_NRD_1	418	420	PF00675	0.707
CLV_NRD_NRD_1	463	465	PF00675	0.692
CLV_NRD_NRD_1	473	475	PF00675	0.714
CLV_PCSK_FUR_1	386	390	PF00082	0.693
CLV_PCSK_KEX2_1	2	4	PF00082	0.495
CLV_PCSK_KEX2_1	32	34	PF00082	0.703
CLV_PCSK_KEX2_1	388	390	PF00082	0.689
CLV_PCSK_KEX2_1	418	420	PF00082	0.677
CLV_PCSK_KEX2_1	463	465	PF00082	0.704
CLV_PCSK_KEX2_1	472	474	PF00082	0.745
CLV_PCSK_PC1ET2_1	388	390	PF00082	0.689
CLV_PCSK_PC1ET2_1	472	474	PF00082	0.792
CLV_PCSK_PC7_1	384	390	PF00082	0.698
CLV_PCSK_SKI1_1	118	122	PF00082	0.688
CLV_PCSK_SKI1_1	157	161	PF00082	0.651
CLV_PCSK_SKI1_1	33	37	PF00082	0.653
CLV_PCSK_SKI1_1	418	422	PF00082	0.617
CLV_PCSK_SKI1_1	474	478	PF00082	0.750
CLV_PCSK_SKI1_1	519	523	PF00082	0.676
CLV_PCSK_SKI1_1	821	825	PF00082	0.650
DEG_APCC_DBOX_1	117	125	PF00400	0.488
DEG_APCC_DBOX_1	417	425	PF00400	0.411
DEG_Nend_UBRbox_1	1	4	PF02207	0.710
DEG_SCF_SKP2-CKS1_1	465	472	PF00560	0.483
DEG_SIAH_1	168	176	PF03145	0.458
DEG_SPOP_SBC_1	229	233	PF00917	0.487
DEG_SPOP_SBC_1	35	39	PF00917	0.478
DEG_SPOP_SBC_1	357	361	PF00917	0.527
DEG_SPOP_SBC_1	374	378	PF00917	0.424
DEG_SPOP_SBC_1	508	512	PF00917	0.479
DEG_SPOP_SBC_1	531	535	PF00917	0.468
DEG_SPOP_SBC_1	599	603	PF00917	0.447
DEG_SPOP_SBC_1	82	86	PF00917	0.427
DEG_SPOP_SBC_1	96	100	PF00917	0.423
DOC_CDC14_PxL_1	314	322	PF14671	0.446
DOC_CYCLIN_yCln2_LP_2	627	633	PF00134	0.421
DOC_MAPK_gen_1	129	138	PF00069	0.390
DOC_MAPK_gen_1	561	570	PF00069	0.452
DOC_MAPK_gen_1	826	833	PF00069	0.352
DOC_PP2B_LxvP_1	17	20	PF13499	0.243
DOC_USP7_MATH_1	143	147	PF00917	0.454
DOC_USP7_MATH_1	186	190	PF00917	0.529
DOC_USP7_MATH_1	194	198	PF00917	0.480
DOC_USP7_MATH_1	271	275	PF00917	0.474
DOC_USP7_MATH_1	277	281	PF00917	0.470
DOC_USP7_MATH_1	28	32	PF00917	0.473
DOC_USP7_MATH_1	290	294	PF00917	0.427
DOC_USP7_MATH_1	331	335	PF00917	0.549
DOC_USP7_MATH_1	508	512	PF00917	0.461
DOC_USP7_MATH_1	513	517	PF00917	0.548
DOC_USP7_MATH_1	532	536	PF00917	0.524
DOC_USP7_MATH_1	58	62	PF00917	0.459
DOC_USP7_MATH_1	597	601	PF00917	0.508
DOC_USP7_MATH_1	606	610	PF00917	0.483
DOC_USP7_MATH_1	611	615	PF00917	0.453
DOC_USP7_MATH_1	633	637	PF00917	0.490
DOC_USP7_MATH_1	644	648	PF00917	0.478
DOC_USP7_MATH_1	703	707	PF00917	0.407
DOC_USP7_MATH_1	791	795	PF00917	0.457
DOC_USP7_MATH_1	82	86	PF00917	0.428
DOC_USP7_MATH_1	97	101	PF00917	0.433
DOC_USP7_UBL2_3	800	804	PF12436	0.387
DOC_WW_Pin1_4	332	337	PF00397	0.516
DOC_WW_Pin1_4	378	383	PF00397	0.484
DOC_WW_Pin1_4	466	471	PF00397	0.486
DOC_WW_Pin1_4	511	516	PF00397	0.481
DOC_WW_Pin1_4	54	59	PF00397	0.499
DOC_WW_Pin1_4	602	607	PF00397	0.536
DOC_WW_Pin1_4	626	631	PF00397	0.607
DOC_WW_Pin1_4	727	732	PF00397	0.431
DOC_WW_Pin1_4	762	767	PF00397	0.320
LIG_14-3-3_CanoR_1	10	20	PF00244	0.493
LIG_14-3-3_CanoR_1	137	144	PF00244	0.389
LIG_14-3-3_CanoR_1	157	166	PF00244	0.420
LIG_14-3-3_CanoR_1	217	226	PF00244	0.466
LIG_14-3-3_CanoR_1	286	295	PF00244	0.441
LIG_14-3-3_CanoR_1	33	43	PF00244	0.511
LIG_14-3-3_CanoR_1	375	381	PF00244	0.498
LIG_14-3-3_CanoR_1	591	597	PF00244	0.466
LIG_14-3-3_CanoR_1	645	649	PF00244	0.417
LIG_14-3-3_CanoR_1	662	672	PF00244	0.253
LIG_14-3-3_CanoR_1	740	745	PF00244	0.471
LIG_14-3-3_CanoR_1	746	754	PF00244	0.432
LIG_Actin_WH2_2	284	299	PF00022	0.434
LIG_BRCT_BRCA1_1	427	431	PF00533	0.426
LIG_DLG_GKlike_1	740	748	PF00625	0.431
LIG_FHA_1	112	118	PF00498	0.468
LIG_FHA_1	251	257	PF00498	0.493
LIG_FHA_1	352	358	PF00498	0.493
LIG_FHA_1	679	685	PF00498	0.330
LIG_FHA_1	7	13	PF00498	0.624
LIG_FHA_1	763	769	PF00498	0.391
LIG_FHA_2	121	127	PF00498	0.410
LIG_FHA_2	179	185	PF00498	0.514
LIG_FHA_2	219	225	PF00498	0.462
LIG_FHA_2	324	330	PF00498	0.450
LIG_FHA_2	445	451	PF00498	0.457
LIG_GBD_Chelix_1	391	399	PF00786	0.677
LIG_LIR_Apic_2	99	105	PF02991	0.428
LIG_LIR_Gen_1	434	442	PF02991	0.451
LIG_LIR_Gen_1	658	668	PF02991	0.408
LIG_LIR_Nem_3	428	433	PF02991	0.446
LIG_LIR_Nem_3	434	438	PF02991	0.421
LIG_LIR_Nem_3	658	663	PF02991	0.415
LIG_MAD2	662	670	PF02301	0.384
LIG_MLH1_MIPbox_1	427	431	PF16413	0.426
LIG_MYND_1	403	407	PF01753	0.452
LIG_MYND_1	732	736	PF01753	0.431
LIG_NRBOX	394	400	PF00104	0.474
LIG_Pex14_2	265	269	PF04695	0.448
LIG_SH2_CRK	102	106	PF00017	0.419
LIG_SH2_NCK_1	435	439	PF00017	0.456
LIG_SH2_NCK_1	726	730	PF00017	0.532
LIG_SH2_NCK_1	744	748	PF00017	0.532
LIG_SH2_SRC	435	438	PF00017	0.459
LIG_SH2_STAP1	435	439	PF00017	0.456
LIG_SH2_STAP1	458	462	PF00017	0.421
LIG_SH2_STAT5	430	433	PF00017	0.451
LIG_SH2_STAT5	654	657	PF00017	0.505
LIG_SH2_STAT5	660	663	PF00017	0.441
LIG_SH2_STAT5	726	729	PF00017	0.532
LIG_SH3_3	223	229	PF00018	0.455
LIG_SH3_3	273	279	PF00018	0.485
LIG_SH3_3	52	58	PF00018	0.482
LIG_SH3_3	541	547	PF00018	0.453
LIG_SH3_3	593	599	PF00018	0.442
LIG_SH3_3	627	633	PF00018	0.544
LIG_SH3_4	525	532	PF00018	0.453
LIG_Sin3_3	244	251	PF02671	0.510
LIG_SUMO_SIM_anti_2	829	836	PF11976	0.357
LIG_SUMO_SIM_par_1	488	493	PF11976	0.429
LIG_SUMO_SIM_par_1	540	545	PF11976	0.469
LIG_TRAF2_1	182	185	PF00917	0.465
LIG_TRAF2_1	777	780	PF00917	0.472
LIG_TYR_ITIM	433	438	PF00017	0.465
LIG_UBA3_1	567	573	PF00899	0.488
LIG_WW_3	730	734	PF00397	0.532
MOD_CDC14_SPxK_1	381	384	PF00782	0.521
MOD_CDC14_SPxK_1	730	733	PF00782	0.431
MOD_CDK_SPxK_1	378	384	PF00069	0.529
MOD_CDK_SPxK_1	466	472	PF00069	0.492
MOD_CDK_SPxK_1	727	733	PF00069	0.431
MOD_CDK_SPxxK_3	332	339	PF00069	0.431
MOD_CDK_SPxxK_3	466	473	PF00069	0.492
MOD_CK1_1	106	112	PF00069	0.437
MOD_CK1_1	145	151	PF00069	0.432
MOD_CK1_1	179	185	PF00069	0.527
MOD_CK1_1	189	195	PF00069	0.485
MOD_CK1_1	196	202	PF00069	0.457
MOD_CK1_1	218	224	PF00069	0.543
MOD_CK1_1	231	237	PF00069	0.432
MOD_CK1_1	285	291	PF00069	0.519
MOD_CK1_1	335	341	PF00069	0.524
MOD_CK1_1	358	364	PF00069	0.469
MOD_CK1_1	373	379	PF00069	0.443
MOD_CK1_1	411	417	PF00069	0.359
MOD_CK1_1	452	458	PF00069	0.426
MOD_CK1_1	50	56	PF00069	0.449
MOD_CK1_1	511	517	PF00069	0.544
MOD_CK1_1	533	539	PF00069	0.469
MOD_CK1_1	549	555	PF00069	0.450
MOD_CK1_1	574	580	PF00069	0.538
MOD_CK1_1	600	606	PF00069	0.592
MOD_CK1_1	619	625	PF00069	0.415
MOD_CK1_1	636	642	PF00069	0.486
MOD_CK1_1	745	751	PF00069	0.537
MOD_CK1_1	771	777	PF00069	0.462
MOD_CK1_1	832	838	PF00069	0.372
MOD_CK1_1	84	90	PF00069	0.479
MOD_CK2_1	106	112	PF00069	0.465
MOD_CK2_1	120	126	PF00069	0.420
MOD_CK2_1	178	184	PF00069	0.523
MOD_CK2_1	218	224	PF00069	0.463
MOD_CK2_1	451	457	PF00069	0.434
MOD_CK2_1	574	580	PF00069	0.437
MOD_GlcNHglycan	106	109	PF01048	0.639
MOD_GlcNHglycan	169	172	PF01048	0.715
MOD_GlcNHglycan	178	181	PF01048	0.685
MOD_GlcNHglycan	198	201	PF01048	0.717
MOD_GlcNHglycan	202	206	PF01048	0.705
MOD_GlcNHglycan	273	276	PF01048	0.657
MOD_GlcNHglycan	279	282	PF01048	0.696
MOD_GlcNHglycan	288	291	PF01048	0.644
MOD_GlcNHglycan	292	295	PF01048	0.610
MOD_GlcNHglycan	346	349	PF01048	0.700
MOD_GlcNHglycan	39	42	PF01048	0.706
MOD_GlcNHglycan	413	416	PF01048	0.631
MOD_GlcNHglycan	495	498	PF01048	0.648
MOD_GlcNHglycan	52	55	PF01048	0.639
MOD_GlcNHglycan	544	547	PF01048	0.695
MOD_GlcNHglycan	573	576	PF01048	0.704
MOD_GlcNHglycan	602	605	PF01048	0.788
MOD_GlcNHglycan	635	638	PF01048	0.704
MOD_GlcNHglycan	641	644	PF01048	0.704
MOD_GlcNHglycan	693	696	PF01048	0.575
MOD_GlcNHglycan	711	714	PF01048	0.218
MOD_GlcNHglycan	770	773	PF01048	0.648
MOD_GSK3_1	139	146	PF00069	0.457
MOD_GSK3_1	158	165	PF00069	0.422
MOD_GSK3_1	176	183	PF00069	0.470
MOD_GSK3_1	18	25	PF00069	0.282
MOD_GSK3_1	184	191	PF00069	0.519
MOD_GSK3_1	192	199	PF00069	0.486
MOD_GSK3_1	201	208	PF00069	0.459
MOD_GSK3_1	215	222	PF00069	0.489
MOD_GSK3_1	228	235	PF00069	0.434
MOD_GSK3_1	267	274	PF00069	0.532
MOD_GSK3_1	282	289	PF00069	0.428
MOD_GSK3_1	325	332	PF00069	0.539
MOD_GSK3_1	33	40	PF00069	0.455
MOD_GSK3_1	340	347	PF00069	0.445
MOD_GSK3_1	351	358	PF00069	0.444
MOD_GSK3_1	370	377	PF00069	0.454
MOD_GSK3_1	393	400	PF00069	0.456
MOD_GSK3_1	46	53	PF00069	0.470
MOD_GSK3_1	507	514	PF00069	0.551
MOD_GSK3_1	515	522	PF00069	0.565
MOD_GSK3_1	530	537	PF00069	0.519
MOD_GSK3_1	54	61	PF00069	0.448
MOD_GSK3_1	542	549	PF00069	0.580
MOD_GSK3_1	598	605	PF00069	0.565
MOD_GSK3_1	6	13	PF00069	0.636
MOD_GSK3_1	606	613	PF00069	0.511
MOD_GSK3_1	632	639	PF00069	0.523
MOD_GSK3_1	693	700	PF00069	0.348
MOD_GSK3_1	829	836	PF00069	0.357
MOD_N-GLC_1	607	612	PF02516	0.731
MOD_N-GLC_1	678	683	PF02516	0.629
MOD_N-GLC_1	829	834	PF02516	0.552
MOD_NEK2_1	136	141	PF00069	0.414
MOD_NEK2_1	178	183	PF00069	0.525
MOD_NEK2_1	230	235	PF00069	0.479
MOD_NEK2_1	296	301	PF00069	0.441
MOD_NEK2_1	362	367	PF00069	0.498
MOD_NEK2_1	465	470	PF00069	0.469
MOD_NEK2_1	509	514	PF00069	0.452
MOD_NEK2_1	571	576	PF00069	0.466
MOD_NEK2_1	592	597	PF00069	0.460
MOD_NEK2_1	677	682	PF00069	0.355
MOD_NEK2_1	693	698	PF00069	0.222
MOD_NEK2_1	768	773	PF00069	0.558
MOD_NEK2_1	833	838	PF00069	0.376
MOD_NEK2_2	644	649	PF00069	0.481
MOD_NEK2_2	97	102	PF00069	0.432
MOD_PIKK_1	143	149	PF00454	0.412
MOD_PIKK_1	312	318	PF00454	0.532
MOD_PIKK_1	408	414	PF00454	0.354
MOD_PIKK_1	697	703	PF00454	0.332
MOD_PIKK_1	745	751	PF00454	0.546
MOD_PIKK_1	771	777	PF00454	0.462
MOD_PK_1	449	455	PF00069	0.445
MOD_PKA_2	136	142	PF00069	0.387
MOD_PKA_2	216	222	PF00069	0.464
MOD_PKA_2	285	291	PF00069	0.458
MOD_PKA_2	374	380	PF00069	0.541
MOD_PKA_2	549	555	PF00069	0.459
MOD_PKA_2	616	622	PF00069	0.446
MOD_PKA_2	644	650	PF00069	0.462
MOD_PKA_2	724	730	PF00069	0.532
MOD_PKA_2	745	751	PF00069	0.441
MOD_Plk_1	312	318	PF00069	0.446
MOD_Plk_1	449	455	PF00069	0.465
MOD_Plk_1	677	683	PF00069	0.332
MOD_Plk_1	829	835	PF00069	0.354
MOD_Plk_2-3	250	256	PF00069	0.488
MOD_Plk_2-3	444	450	PF00069	0.354
MOD_Plk_4	232	238	PF00069	0.538
MOD_Plk_4	341	347	PF00069	0.443
MOD_Plk_4	650	656	PF00069	0.408
MOD_Plk_4	678	684	PF00069	0.353
MOD_Plk_4	829	835	PF00069	0.370
MOD_Plk_4	89	95	PF00069	0.496
MOD_Plk_4	97	103	PF00069	0.456
MOD_ProDKin_1	332	338	PF00069	0.516
MOD_ProDKin_1	378	384	PF00069	0.482
MOD_ProDKin_1	466	472	PF00069	0.492
MOD_ProDKin_1	511	517	PF00069	0.482
MOD_ProDKin_1	54	60	PF00069	0.497
MOD_ProDKin_1	602	608	PF00069	0.536
MOD_ProDKin_1	626	632	PF00069	0.607
MOD_ProDKin_1	727	733	PF00069	0.431
MOD_ProDKin_1	762	768	PF00069	0.320
MOD_SUMO_for_1	130	133	PF00179	0.466
MOD_SUMO_rev_2	517	527	PF00179	0.456
MOD_SUMO_rev_2	815	820	PF00179	0.344
TRG_DiLeu_BaEn_1	416	421	PF01217	0.414
TRG_DiLeu_BaLyEn_6	763	768	PF01217	0.385
TRG_DiLeu_LyEn_5	416	421	PF01217	0.414
TRG_ENDOCYTIC_2	430	433	PF00928	0.451
TRG_ENDOCYTIC_2	435	438	PF00928	0.419
TRG_ENDOCYTIC_2	660	663	PF00928	0.497
TRG_ER_diArg_1	1	3	PF00400	0.704
TRG_ER_diArg_1	383	386	PF00400	0.513
TRG_ER_diArg_1	418	420	PF00400	0.463
TRG_ER_diArg_1	462	464	PF00400	0.481
TRG_ER_diArg_1	784	787	PF00400	0.403
TRG_NLS_MonoCore_2	239	244	PF00514	0.506
TRG_NLS_MonoExtN_4	238	244	PF00514	0.509
TRG_NLS_MonoExtN_4	470	476	PF00514	0.566
TRG_Pf-PMV_PEXEL_1	122	126	PF00026	0.688

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3S5H678	Leishmania donovani	68%	100%
A4HTN7	Leishmania infantum	68%	100%
E9AMH1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	66%	100%
Q4QI72	Leishmania major	70%	100%