DNA polymerase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA polymerase

Gene product:

mitochondrial DNA polymerase beta

Species:

Leishmania braziliensis

UniProt:

A4H5D4_LEIBR

TriTrypDb:

LbrM.08.0710 , LBRM2903_080014500

Length:

376

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	13
GO:0005737	cytoplasm	2	13
GO:0043226	organelle	2	13
GO:0043227	membrane-bounded organelle	3	13
GO:0043229	intracellular organelle	3	13
GO:0043231	intracellular membrane-bounded organelle	4	13
GO:0110165	cellular anatomical entity	1	13

Expansion

Sequence features

A4H5D4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H5D4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006259	DNA metabolic process	4	13
GO:0006281	DNA repair	5	13
GO:0006396	RNA processing	6	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0006950	response to stress	2	13
GO:0006974	DNA damage response	4	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0031123	RNA 3'-end processing	7	13
GO:0033554	cellular response to stress	3	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0043170	macromolecule metabolic process	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044260	obsolete cellular macromolecule metabolic process	3	13
GO:0046483	heterocycle metabolic process	3	13
GO:0050896	response to stimulus	1	13
GO:0051716	cellular response to stimulus	2	13
GO:0071076	RNA 3' uridylation	8	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13
GO:0006302	double-strand break repair	6	1
GO:0006303	double-strand break repair via nonhomologous end joining	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	13
GO:0003677	DNA binding	4	13
GO:0003824	catalytic activity	1	13
GO:0003887	DNA-directed DNA polymerase activity	5	13
GO:0005488	binding	1	13
GO:0016740	transferase activity	2	13
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	13
GO:0016779	nucleotidyltransferase activity	4	13
GO:0034061	DNA polymerase activity	4	13
GO:0043167	ion binding	2	13
GO:0043169	cation binding	3	13
GO:0046872	metal ion binding	4	13
GO:0050265	RNA uridylyltransferase activity	4	13
GO:0070569	uridylyltransferase activity	5	13
GO:0097159	organic cyclic compound binding	2	13
GO:0140097	catalytic activity, acting on DNA	3	13
GO:0140098	catalytic activity, acting on RNA	3	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13
GO:1901363	heterocyclic compound binding	2	13
GO:0016829	lyase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	187	189	PF00675	0.226
CLV_NRD_NRD_1	268	270	PF00675	0.218
CLV_NRD_NRD_1	8	10	PF00675	0.431
CLV_PCSK_KEX2_1	186	188	PF00082	0.225
CLV_PCSK_KEX2_1	268	270	PF00082	0.218
CLV_PCSK_KEX2_1	350	352	PF00082	0.227
CLV_PCSK_KEX2_1	8	10	PF00082	0.446
CLV_PCSK_PC1ET2_1	350	352	PF00082	0.227
CLV_PCSK_SKI1_1	68	72	PF00082	0.233
DEG_COP1_1	276	283	PF00400	0.426
DEG_Nend_Nbox_1	1	3	PF02207	0.538
DOC_CKS1_1	316	321	PF01111	0.524
DOC_CKS1_1	53	58	PF01111	0.524
DOC_CYCLIN_yClb5_NLxxxL_5	46	53	PF00134	0.452
DOC_CYCLIN_yCln2_LP_2	208	214	PF00134	0.470
DOC_MAPK_gen_1	263	272	PF00069	0.428
DOC_MAPK_gen_1	8	18	PF00069	0.378
DOC_MAPK_MEF2A_6	266	274	PF00069	0.428
DOC_PP2B_LxvP_1	203	206	PF13499	0.452
DOC_PP2B_LxvP_1	340	343	PF13499	0.524
DOC_PP2B_LxvP_1	364	367	PF13499	0.438
DOC_USP7_UBL2_3	68	72	PF12436	0.427
DOC_USP7_UBL2_3	88	92	PF12436	0.422
DOC_WW_Pin1_4	315	320	PF00397	0.452
DOC_WW_Pin1_4	52	57	PF00397	0.526
LIG_14-3-3_CanoR_1	3	7	PF00244	0.525
LIG_Actin_WH2_2	58	73	PF00022	0.357
LIG_Actin_WH2_2	78	96	PF00022	0.406
LIG_APCC_ABBA_1	16	21	PF00400	0.438
LIG_BIR_III_2	204	208	PF00653	0.522
LIG_BRCT_BRCA1_1	289	293	PF00533	0.418
LIG_EH1_1	119	127	PF00400	0.438
LIG_FHA_1	213	219	PF00498	0.445
LIG_FHA_1	275	281	PF00498	0.509
LIG_FHA_1	77	83	PF00498	0.371
LIG_FHA_2	119	125	PF00498	0.424
LIG_FHA_2	316	322	PF00498	0.492
LIG_FHA_2	45	51	PF00498	0.431
LIG_FHA_2	53	59	PF00498	0.433
LIG_LIR_Gen_1	176	185	PF02991	0.529
LIG_LIR_Gen_1	290	301	PF02991	0.438
LIG_LIR_Gen_1	359	369	PF02991	0.442
LIG_LIR_Nem_3	176	180	PF02991	0.529
LIG_LIR_Nem_3	290	296	PF02991	0.438
LIG_LIR_Nem_3	310	314	PF02991	0.437
LIG_LIR_Nem_3	359	364	PF02991	0.442
LIG_SH2_NCK_1	326	330	PF00017	0.515
LIG_SH2_NCK_1	368	372	PF00017	0.493
LIG_SH2_SRC	230	233	PF00017	0.444
LIG_SH2_STAT5	145	148	PF00017	0.438
LIG_SH2_STAT5	164	167	PF00017	0.526
LIG_SH2_STAT5	230	233	PF00017	0.454
LIG_SH2_STAT5	306	309	PF00017	0.438
LIG_SH3_4	90	97	PF00018	0.400
LIG_TRAF2_1	154	157	PF00917	0.524
LIG_TRAF2_1	318	321	PF00917	0.452
LIG_TRAF2_1	336	339	PF00917	0.494
LIG_TRAF2_1	95	98	PF00917	0.382
LIG_WRC_WIRS_1	284	289	PF05994	0.438
LIG_WW_3	248	252	PF00397	0.438
MOD_CK1_1	131	137	PF00069	0.524
MOD_CK1_1	279	285	PF00069	0.336
MOD_CK1_1	41	47	PF00069	0.434
MOD_CK2_1	118	124	PF00069	0.443
MOD_CK2_1	315	321	PF00069	0.437
MOD_GlcNHglycan	191	194	PF01048	0.218
MOD_GlcNHglycan	62	65	PF01048	0.327
MOD_GSK3_1	279	286	PF00069	0.344
MOD_GSK3_1	34	41	PF00069	0.509
MOD_NEK2_1	155	160	PF00069	0.438
MOD_NEK2_1	2	7	PF00069	0.526
MOD_NEK2_1	224	229	PF00069	0.463
MOD_NEK2_1	231	236	PF00069	0.414
MOD_NEK2_1	274	279	PF00069	0.495
MOD_NEK2_1	285	290	PF00069	0.384
MOD_PIKK_1	97	103	PF00454	0.438
MOD_PK_1	128	134	PF00069	0.496
MOD_PK_1	175	181	PF00069	0.524
MOD_PKA_2	2	8	PF00069	0.519
MOD_Plk_1	175	181	PF00069	0.532
MOD_Plk_1	231	237	PF00069	0.437
MOD_Plk_1	97	103	PF00069	0.438
MOD_Plk_2-3	353	359	PF00069	0.524
MOD_Plk_4	155	161	PF00069	0.434
MOD_ProDKin_1	315	321	PF00069	0.452
MOD_ProDKin_1	52	58	PF00069	0.526
MOD_SUMO_for_1	343	346	PF00179	0.462
MOD_SUMO_for_1	369	372	PF00179	0.438
MOD_SUMO_rev_2	271	280	PF00179	0.470
MOD_SUMO_rev_2	345	352	PF00179	0.438
MOD_SUMO_rev_2	83	89	PF00179	0.429
TRG_DiLeu_BaEn_4	320	326	PF01217	0.452
TRG_DiLeu_BaLyEn_6	270	275	PF01217	0.438
TRG_ENDOCYTIC_2	242	245	PF00928	0.430
TRG_ENDOCYTIC_2	311	314	PF00928	0.421
TRG_ENDOCYTIC_2	326	329	PF00928	0.406
TRG_ER_diArg_1	185	188	PF00400	0.435
TRG_ER_diArg_1	268	270	PF00400	0.418
TRG_ER_diArg_1	7	9	PF00400	0.491
TRG_Pf-PMV_PEXEL_1	323	327	PF00026	0.238

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HU90	Leptomonas seymouri	92%	100%
A0A0S4J2Q4	Bodo saltans	31%	100%
A0A0S4J8Y1	Bodo saltans	38%	100%
A0A1X0NP40	Trypanosomatidae	71%	93%
A0A3R7LYF2	Trypanosoma rangeli	69%	94%
A0A3S7WQD2	Leishmania donovani	98%	100%
A4HTM9	Leishmania infantum	98%	100%
C9ZPC6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	67%	92%
E9AMG3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
O57383	Xenopus laevis	31%	100%
P06746	Homo sapiens	30%	100%
P06766	Rattus norvegicus	31%	100%
Q09693	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	22%	74%
Q27958	Bos taurus	30%	100%
Q4QI80	Leishmania major	98%	100%
Q67VC8	Oryza sativa subsp. japonica	30%	68%
Q6DRD3	Danio rerio	30%	100%
Q7T6Y4	Acanthamoeba polyphaga mimivirus	30%	100%
Q8K409	Mus musculus	31%	100%
Q9FNY4	Arabidopsis thaliana	28%	71%
Q9JIW4	Mus musculus	22%	76%
V5BGW3	Trypanosoma cruzi	69%	93%