Calcium-transporting ATPase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

vacuolar-type Ca2+-ATPase, putative

Species:

Leishmania braziliensis

UniProt:

A4H516_LEIBR

TriTrypDb:

LbrM.07.0720 , LBRM2903_040005000 , LBRM2903_070014100

Length:

592

Annotations

LeishMANIAdb annotations

Homologous to other eukaryotic P-type Ca2+ ATPases.. For some reason, this group has heavily expanded in Kinetoplastida.. Localization: Endosomal (by homology) / ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 2
NetGPI	no	yes: 0, no: 2

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

A4H516

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H516

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	3
GO:0003824	catalytic activity	1	3
GO:0005215	transporter activity	1	3
GO:0005388	P-type calcium transporter activity	4	3
GO:0005488	binding	1	3
GO:0005524	ATP binding	5	3
GO:0008324	monoatomic cation transmembrane transporter activity	4	3
GO:0015075	monoatomic ion transmembrane transporter activity	3	3
GO:0015085	calcium ion transmembrane transporter activity	6	3
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	3
GO:0015399	primary active transmembrane transporter activity	4	3
GO:0015662	P-type ion transporter activity	4	3
GO:0016462	pyrophosphatase activity	5	3
GO:0016787	hydrolase activity	2	3
GO:0016817	hydrolase activity, acting on acid anhydrides	3	3
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	3
GO:0016887	ATP hydrolysis activity	7	3
GO:0017076	purine nucleotide binding	4	3
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	3
GO:0019829	ATPase-coupled monoatomic cation transmembrane transporter activity	3	3
GO:0022804	active transmembrane transporter activity	3	3
GO:0022853	active monoatomic ion transmembrane transporter activity	4	3
GO:0022857	transmembrane transporter activity	2	3
GO:0022890	inorganic cation transmembrane transporter activity	4	3
GO:0030554	adenyl nucleotide binding	5	3
GO:0032553	ribonucleotide binding	3	3
GO:0032555	purine ribonucleotide binding	4	3
GO:0032559	adenyl ribonucleotide binding	5	3
GO:0035639	purine ribonucleoside triphosphate binding	4	3
GO:0036094	small molecule binding	2	3
GO:0042626	ATPase-coupled transmembrane transporter activity	2	3
GO:0043167	ion binding	2	3
GO:0043168	anion binding	3	3
GO:0046873	metal ion transmembrane transporter activity	5	3
GO:0097159	organic cyclic compound binding	2	3
GO:0097367	carbohydrate derivative binding	2	3
GO:0140358	P-type transmembrane transporter activity	3	3
GO:0140657	ATP-dependent activity	1	3
GO:1901265	nucleoside phosphate binding	3	3
GO:1901363	heterocyclic compound binding	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	136	140	PF00656	0.625
CLV_C14_Caspase3-7	2	6	PF00656	0.805
CLV_NRD_NRD_1	17	19	PF00675	0.394
CLV_NRD_NRD_1	294	296	PF00675	0.335
CLV_NRD_NRD_1	34	36	PF00675	0.246
CLV_NRD_NRD_1	362	364	PF00675	0.300
CLV_NRD_NRD_1	370	372	PF00675	0.300
CLV_NRD_NRD_1	427	429	PF00675	0.300
CLV_NRD_NRD_1	550	552	PF00675	0.378
CLV_NRD_NRD_1	576	578	PF00675	0.404
CLV_PCSK_KEX2_1	357	359	PF00082	0.360
CLV_PCSK_KEX2_1	36	38	PF00082	0.300
CLV_PCSK_KEX2_1	370	372	PF00082	0.240
CLV_PCSK_KEX2_1	550	552	PF00082	0.500
CLV_PCSK_KEX2_1	576	578	PF00082	0.406
CLV_PCSK_PC1ET2_1	357	359	PF00082	0.425
CLV_PCSK_PC1ET2_1	36	38	PF00082	0.300
CLV_PCSK_SKI1_1	199	203	PF00082	0.425
CLV_PCSK_SKI1_1	221	225	PF00082	0.300
CLV_PCSK_SKI1_1	24	28	PF00082	0.425
CLV_PCSK_SKI1_1	295	299	PF00082	0.328
CLV_PCSK_SKI1_1	520	524	PF00082	0.492
DOC_CYCLIN_RxL_1	293	303	PF00134	0.530
DOC_MAPK_gen_1	295	302	PF00069	0.530
DOC_MAPK_gen_1	370	376	PF00069	0.500
DOC_MAPK_MEF2A_6	221	228	PF00069	0.500
DOC_MAPK_MEF2A_6	295	304	PF00069	0.500
DOC_MAPK_MEF2A_6	370	378	PF00069	0.542
DOC_MAPK_MEF2A_6	443	450	PF00069	0.500
DOC_MAPK_NFAT4_5	221	229	PF00069	0.500
DOC_MAPK_NFAT4_5	295	303	PF00069	0.500
DOC_PP1_RVXF_1	280	287	PF00149	0.545
DOC_USP7_MATH_1	264	268	PF00917	0.545
DOC_USP7_MATH_1	30	34	PF00917	0.500
DOC_USP7_MATH_1	366	370	PF00917	0.625
DOC_WW_Pin1_4	321	326	PF00397	0.385
DOC_WW_Pin1_4	441	446	PF00397	0.542
LIG_APCC_ABBA_1	462	467	PF00400	0.408
LIG_APCC_ABBAyCdc20_2	541	547	PF00400	0.701
LIG_BIR_III_4	192	196	PF00653	0.625
LIG_BRCT_BRCA1_1	418	422	PF00533	0.408
LIG_BRCT_BRCA1_1	443	447	PF00533	0.491
LIG_FHA_1	116	122	PF00498	0.454
LIG_FHA_1	326	332	PF00498	0.300
LIG_FHA_1	97	103	PF00498	0.672
LIG_FHA_2	180	186	PF00498	0.625
LIG_FHA_2	228	234	PF00498	0.625
LIG_FHA_2	534	540	PF00498	0.633
LIG_FHA_2	576	582	PF00498	0.646
LIG_FHA_2	69	75	PF00498	0.579
LIG_IBAR_NPY_1	173	175	PF08397	0.625
LIG_KLC1_Yacidic_2	542	547	PF13176	0.695
LIG_LIR_Gen_1	303	314	PF02991	0.525
LIG_LIR_Gen_1	410	421	PF02991	0.350
LIG_LIR_Gen_1	432	441	PF02991	0.531
LIG_LIR_Gen_1	467	474	PF02991	0.282
LIG_LIR_Nem_3	185	189	PF02991	0.500
LIG_LIR_Nem_3	274	280	PF02991	0.543
LIG_LIR_Nem_3	303	309	PF02991	0.525
LIG_LIR_Nem_3	410	416	PF02991	0.350
LIG_LIR_Nem_3	419	425	PF02991	0.350
LIG_LIR_Nem_3	432	437	PF02991	0.467
LIG_LIR_Nem_3	444	450	PF02991	0.455
LIG_LIR_Nem_3	463	468	PF02991	0.371
LIG_NRBOX	436	442	PF00104	0.542
LIG_PCNA_yPIPBox_3	288	298	PF02747	0.535
LIG_Pex14_2	453	457	PF04695	0.381
LIG_Pex14_2	506	510	PF04695	0.605
LIG_PTB_Apo_2	170	177	PF02174	0.625
LIG_PTB_Apo_2	185	192	PF02174	0.438
LIG_PTB_Apo_2	249	256	PF02174	0.538
LIG_PTB_Apo_2	392	399	PF02174	0.342
LIG_PTB_Phospho_1	170	176	PF10480	0.625
LIG_PTB_Phospho_1	185	191	PF10480	0.438
LIG_SH2_CRK	104	108	PF00017	0.700
LIG_SH2_CRK	393	397	PF00017	0.342
LIG_SH2_GRB2like	67	70	PF00017	0.707
LIG_SH2_PTP2	306	309	PF00017	0.525
LIG_SH2_STAT5	306	309	PF00017	0.525
LIG_SH2_STAT5	545	548	PF00017	0.686
LIG_SH2_STAT5	67	70	PF00017	0.657
LIG_SH3_3	129	135	PF00018	0.575
LIG_SH3_3	498	504	PF00018	0.636
LIG_SUMO_SIM_anti_2	269	275	PF11976	0.556
LIG_SUMO_SIM_par_1	224	230	PF11976	0.536
LIG_SUMO_SIM_par_1	233	238	PF11976	0.464
LIG_SUMO_SIM_par_1	269	275	PF11976	0.556
LIG_SUMO_SIM_par_1	458	463	PF11976	0.408
LIG_TRAF2_1	347	350	PF00917	0.525
LIG_TRAF2_1	351	354	PF00917	0.575
LIG_TRAF2_1	536	539	PF00917	0.642
LIG_TYR_ITIM	102	107	PF00017	0.598
LIG_TYR_ITIM	304	309	PF00017	0.525
LIG_UBA3_1	421	429	PF00899	0.315
LIG_WRC_WIRS_1	450	455	PF05994	0.525
MOD_CK1_1	324	330	PF00069	0.394
MOD_CK1_1	473	479	PF00069	0.408
MOD_CK2_1	105	111	PF00069	0.465
MOD_CK2_1	227	233	PF00069	0.525
MOD_CK2_1	533	539	PF00069	0.558
MOD_CK2_1	68	74	PF00069	0.457
MOD_GlcNHglycan	107	110	PF01048	0.559
MOD_GlcNHglycan	258	261	PF01048	0.434
MOD_GlcNHglycan	266	269	PF01048	0.430
MOD_GlcNHglycan	38	41	PF01048	0.350
MOD_GSK3_1	235	242	PF00069	0.350
MOD_GSK3_1	305	312	PF00069	0.350
MOD_GSK3_1	320	327	PF00069	0.468
MOD_GSK3_1	345	352	PF00069	0.350
MOD_GSK3_1	469	476	PF00069	0.525
MOD_GSK3_1	502	509	PF00069	0.543
MOD_LATS_1	52	58	PF00433	0.525
MOD_N-GLC_1	68	73	PF02516	0.458
MOD_N-GLC_1	96	101	PF02516	0.596
MOD_NEK2_1	179	184	PF00069	0.387
MOD_NEK2_1	213	218	PF00069	0.350
MOD_NEK2_1	227	232	PF00069	0.350
MOD_NEK2_1	300	305	PF00069	0.350
MOD_NEK2_1	313	318	PF00069	0.350
MOD_NEK2_1	372	377	PF00069	0.350
MOD_NEK2_1	384	389	PF00069	0.350
MOD_NEK2_1	416	421	PF00069	0.408
MOD_NEK2_1	469	474	PF00069	0.350
MOD_NEK2_1	510	515	PF00069	0.663
MOD_NEK2_1	566	571	PF00069	0.668
MOD_NEK2_1	575	580	PF00069	0.514
MOD_NEK2_2	147	152	PF00069	0.525
MOD_PIKK_1	213	219	PF00454	0.350
MOD_PKA_1	36	42	PF00069	0.350
MOD_PKA_2	36	42	PF00069	0.350
MOD_PKA_2	575	581	PF00069	0.552
MOD_Plk_1	406	412	PF00069	0.525
MOD_Plk_1	506	512	PF00069	0.669
MOD_Plk_1	96	102	PF00069	0.596
MOD_Plk_2-3	349	355	PF00069	0.525
MOD_Plk_4	122	128	PF00069	0.356
MOD_Plk_4	208	214	PF00069	0.381
MOD_Plk_4	309	315	PF00069	0.369
MOD_Plk_4	372	378	PF00069	0.401
MOD_Plk_4	407	413	PF00069	0.350
MOD_Plk_4	417	423	PF00069	0.350
MOD_Plk_4	45	51	PF00069	0.525
MOD_Plk_4	510	516	PF00069	0.531
MOD_ProDKin_1	321	327	PF00069	0.350
MOD_ProDKin_1	441	447	PF00069	0.408
MOD_SUMO_rev_2	3	11	PF00179	0.552
MOD_SUMO_rev_2	33	38	PF00179	0.350
MOD_SUMO_rev_2	513	522	PF00179	0.463
TRG_DiLeu_BaEn_1	45	50	PF01217	0.525
TRG_DiLeu_BaEn_4	432	438	PF01217	0.408
TRG_DiLeu_BaEn_4	584	590	PF01217	0.716
TRG_DiLeu_BaLyEn_6	494	499	PF01217	0.350
TRG_ENDOCYTIC_2	104	107	PF00928	0.609
TRG_ENDOCYTIC_2	176	179	PF00928	0.455
TRG_ENDOCYTIC_2	306	309	PF00928	0.525
TRG_ENDOCYTIC_2	393	396	PF00928	0.525
TRG_ENDOCYTIC_2	431	434	PF00928	0.434
TRG_ER_diArg_1	35	38	PF00400	0.350
TRG_ER_diArg_1	370	372	PF00400	0.350
TRG_ER_diArg_1	550	553	PF00400	0.593
TRG_ER_diArg_1	575	577	PF00400	0.543
TRG_NES_CRM1_1	119	131	PF08389	0.525
TRG_NES_CRM1_1	217	233	PF08389	0.350
TRG_Pf-PMV_PEXEL_1	187	192	PF00026	0.525
TRG_Pf-PMV_PEXEL_1	428	432	PF00026	0.350
TRG_Pf-PMV_PEXEL_1	555	560	PF00026	0.466

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A4HT82	Leishmania infantum	89%	69%
O34431	Bacillus subtilis (strain 168)	33%	67%
P28877	Candida albicans	24%	66%
P47317	Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37)	30%	68%
Q73E41	Bacillus cereus (strain ATCC 10987 / NRS 248)	31%	67%
Q9CFU9	Lactococcus lactis subsp. lactis (strain IL1403)	30%	67%