Putative tRNA-methyl transferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA-methyl transferase, putative

Species:

Leishmania braziliensis

UniProt:

A4H4V6_LEIBR

TriTrypDb:

LbrM.07.0130 , LBRM2903_070006500

Length:

593

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A4H4V6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4V6

Function

Biological processes
Term	Name	Level	Count
GO:0002097	tRNA wobble base modification	7	1
GO:0002098	tRNA wobble uridine modification	8	1
GO:0002143	tRNA wobble position uridine thiolation	8	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0034227	tRNA thio-modification	7	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004808	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity	6	8
GO:0008168	methyltransferase activity	4	8
GO:0008173	RNA methyltransferase activity	4	8
GO:0008175	tRNA methyltransferase activity	5	8
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	8
GO:0016740	transferase activity	2	10
GO:0016741	transferase activity, transferring one-carbon groups	3	8
GO:0140098	catalytic activity, acting on RNA	3	8
GO:0140101	catalytic activity, acting on a tRNA	4	8
GO:0140640	catalytic activity, acting on a nucleic acid	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	559	563	PF00656	0.635
CLV_NRD_NRD_1	183	185	PF00675	0.296
CLV_NRD_NRD_1	399	401	PF00675	0.612
CLV_PCSK_KEX2_1	183	185	PF00082	0.296
CLV_PCSK_KEX2_1	399	401	PF00082	0.612
CLV_PCSK_KEX2_1	76	78	PF00082	0.476
CLV_PCSK_PC1ET2_1	76	78	PF00082	0.429
CLV_PCSK_SKI1_1	473	477	PF00082	0.302
CLV_PCSK_SKI1_1	531	535	PF00082	0.266
CLV_PCSK_SKI1_1	70	74	PF00082	0.663
DEG_APCC_DBOX_1	60	68	PF00400	0.278
DOC_ANK_TNKS_1	398	405	PF00023	0.581
DOC_CKS1_1	71	76	PF01111	0.537
DOC_CKS1_1	90	95	PF01111	0.496
DOC_CYCLIN_RxL_1	121	132	PF00134	0.518
DOC_CYCLIN_yCln2_LP_2	465	471	PF00134	0.435
DOC_MAPK_gen_1	286	294	PF00069	0.454
DOC_MAPK_gen_1	518	526	PF00069	0.536
DOC_MAPK_MEF2A_6	3	12	PF00069	0.435
DOC_MAPK_MEF2A_6	462	469	PF00069	0.406
DOC_MAPK_NFAT4_5	462	470	PF00069	0.264
DOC_PP1_RVXF_1	170	176	PF00149	0.536
DOC_PP1_RVXF_1	521	527	PF00149	0.496
DOC_PP2B_LxvP_1	321	324	PF13499	0.534
DOC_PP2B_LxvP_1	345	348	PF13499	0.495
DOC_PP2B_LxvP_1	465	468	PF13499	0.564
DOC_PP4_FxxP_1	294	297	PF00568	0.496
DOC_PP4_FxxP_1	41	44	PF00568	0.496
DOC_PP4_FxxP_1	526	529	PF00568	0.442
DOC_USP7_MATH_1	135	139	PF00917	0.384
DOC_USP7_MATH_1	245	249	PF00917	0.384
DOC_USP7_MATH_1	267	271	PF00917	0.536
DOC_USP7_MATH_1	541	545	PF00917	0.451
DOC_USP7_MATH_1	585	589	PF00917	0.482
DOC_WW_Pin1_4	1	6	PF00397	0.364
DOC_WW_Pin1_4	131	136	PF00397	0.512
DOC_WW_Pin1_4	367	372	PF00397	0.451
DOC_WW_Pin1_4	531	536	PF00397	0.480
DOC_WW_Pin1_4	581	586	PF00397	0.394
DOC_WW_Pin1_4	70	75	PF00397	0.505
DOC_WW_Pin1_4	84	89	PF00397	0.568
LIG_14-3-3_CanoR_1	223	231	PF00244	0.459
LIG_14-3-3_CanoR_1	27	35	PF00244	0.435
LIG_14-3-3_CanoR_1	303	313	PF00244	0.402
LIG_14-3-3_CanoR_1	366	373	PF00244	0.453
LIG_14-3-3_CanoR_1	473	482	PF00244	0.442
LIG_APCC_ABBAyCdc20_2	521	527	PF00400	0.479
LIG_BIR_III_2	357	361	PF00653	0.483
LIG_BIR_III_2	562	566	PF00653	0.568
LIG_BIR_III_2	85	89	PF00653	0.440
LIG_BRCT_BRCA1_1	137	141	PF00533	0.388
LIG_BRCT_BRCA1_1	247	251	PF00533	0.384
LIG_BRCT_BRCA1_1	309	313	PF00533	0.513
LIG_BRCT_BRCA1_1	369	373	PF00533	0.454
LIG_CSL_BTD_1	463	466	PF09270	0.460
LIG_FHA_1	123	129	PF00498	0.511
LIG_FHA_1	160	166	PF00498	0.442
LIG_FHA_1	177	183	PF00498	0.543
LIG_FHA_1	200	206	PF00498	0.485
LIG_FHA_1	354	360	PF00498	0.668
LIG_FHA_1	541	547	PF00498	0.512
LIG_FHA_1	568	574	PF00498	0.281
LIG_FHA_2	29	35	PF00498	0.404
LIG_FHA_2	339	345	PF00498	0.507
LIG_Integrin_RGD_1	207	209	PF01839	0.315
LIG_LIR_Apic_2	319	323	PF02991	0.449
LIG_LIR_Apic_2	460	466	PF02991	0.474
LIG_LIR_Apic_2	576	581	PF02991	0.436
LIG_LIR_Gen_1	147	158	PF02991	0.471
LIG_LIR_Nem_3	310	316	PF02991	0.575
LIG_LIR_Nem_3	370	376	PF02991	0.555
LIG_Pex14_2	95	99	PF04695	0.442
LIG_SH2_CRK	542	546	PF00017	0.537
LIG_SH2_NCK_1	116	120	PF00017	0.473
LIG_SH2_SRC	116	119	PF00017	0.536
LIG_SH2_SRC	320	323	PF00017	0.534
LIG_SH2_STAP1	199	203	PF00017	0.456
LIG_SH2_STAP1	387	391	PF00017	0.367
LIG_SH2_STAP1	452	456	PF00017	0.463
LIG_SH2_STAP1	542	546	PF00017	0.422
LIG_SH2_STAT3	145	148	PF00017	0.536
LIG_SH2_STAT3	452	455	PF00017	0.432
LIG_SH2_STAT5	320	323	PF00017	0.482
LIG_SH2_STAT5	412	415	PF00017	0.355
LIG_SH2_STAT5	452	455	PF00017	0.359
LIG_SH2_STAT5	542	545	PF00017	0.494
LIG_SH2_STAT5	91	94	PF00017	0.435
LIG_SH3_1	320	326	PF00018	0.496
LIG_SH3_3	292	298	PF00018	0.536
LIG_SH3_3	320	326	PF00018	0.548
LIG_SH3_3	41	47	PF00018	0.616
LIG_SH3_3	414	420	PF00018	0.502
LIG_SH3_3	562	568	PF00018	0.617
LIG_SH3_3	584	590	PF00018	0.458
LIG_SUMO_SIM_anti_2	125	130	PF11976	0.427
LIG_SUMO_SIM_par_1	543	550	PF11976	0.509
LIG_TRAF2_1	341	344	PF00917	0.593
LIG_TRAF2_1	507	510	PF00917	0.505
LIG_TRAF2_1	547	550	PF00917	0.320
LIG_TYR_ITIM	540	545	PF00017	0.318
LIG_UBA3_1	67	76	PF00899	0.389
LIG_WRC_WIRS_1	313	318	PF05994	0.322
LIG_WW_2	49	52	PF00397	0.337
LIG_WW_3	322	326	PF00397	0.356
MOD_CDC14_SPxK_1	4	7	PF00782	0.300
MOD_CDK_SPxK_1	1	7	PF00069	0.351
MOD_CDK_SPxK_1	70	76	PF00069	0.426
MOD_CDK_SPxxK_3	70	77	PF00069	0.431
MOD_CK1_1	187	193	PF00069	0.361
MOD_CK1_1	299	305	PF00069	0.684
MOD_CK1_1	308	314	PF00069	0.647
MOD_CK1_1	331	337	PF00069	0.481
MOD_CK1_1	424	430	PF00069	0.663
MOD_CK1_1	454	460	PF00069	0.487
MOD_CK2_1	338	344	PF00069	0.504
MOD_Cter_Amidation	74	77	PF01082	0.453
MOD_DYRK1A_RPxSP_1	531	535	PF00069	0.347
MOD_GlcNHglycan	105	108	PF01048	0.439
MOD_GlcNHglycan	12	15	PF01048	0.362
MOD_GlcNHglycan	17	20	PF01048	0.352
MOD_GlcNHglycan	186	189	PF01048	0.356
MOD_GlcNHglycan	225	228	PF01048	0.297
MOD_GlcNHglycan	243	246	PF01048	0.297
MOD_GlcNHglycan	247	250	PF01048	0.419
MOD_GlcNHglycan	273	276	PF01048	0.353
MOD_GlcNHglycan	298	301	PF01048	0.650
MOD_GlcNHglycan	423	426	PF01048	0.642
MOD_GlcNHglycan	508	514	PF01048	0.379
MOD_GlcNHglycan	575	578	PF01048	0.384
MOD_GlcNHglycan	587	590	PF01048	0.503
MOD_GSK3_1	131	138	PF00069	0.358
MOD_GSK3_1	189	196	PF00069	0.389
MOD_GSK3_1	199	206	PF00069	0.297
MOD_GSK3_1	232	239	PF00069	0.328
MOD_GSK3_1	241	248	PF00069	0.337
MOD_GSK3_1	267	274	PF00069	0.355
MOD_GSK3_1	304	311	PF00069	0.612
MOD_GSK3_1	450	457	PF00069	0.429
MOD_GSK3_1	581	588	PF00069	0.415
MOD_LATS_1	471	477	PF00433	0.302
MOD_N-GLC_1	203	208	PF02516	0.197
MOD_N-GLC_1	305	310	PF02516	0.613
MOD_N-GLC_1	556	561	PF02516	0.481
MOD_NEK2_1	168	173	PF00069	0.431
MOD_NEK2_1	182	187	PF00069	0.261
MOD_NEK2_1	189	194	PF00069	0.330
MOD_NEK2_1	271	276	PF00069	0.405
MOD_NEK2_1	328	333	PF00069	0.562
MOD_NEK2_1	450	455	PF00069	0.375
MOD_PIKK_1	451	457	PF00454	0.412
MOD_PKA_1	286	292	PF00069	0.282
MOD_PKA_1	76	82	PF00069	0.430
MOD_PKA_2	182	188	PF00069	0.355
MOD_PKA_2	365	371	PF00069	0.474
MOD_PKA_2	398	404	PF00069	0.608
MOD_PKA_2	517	523	PF00069	0.244
MOD_PKA_2	76	82	PF00069	0.529
MOD_Plk_1	232	238	PF00069	0.279
MOD_Plk_4	189	195	PF00069	0.388
MOD_Plk_4	232	238	PF00069	0.321
MOD_Plk_4	312	318	PF00069	0.395
MOD_Plk_4	359	365	PF00069	0.483
MOD_Plk_4	541	547	PF00069	0.376
MOD_ProDKin_1	1	7	PF00069	0.351
MOD_ProDKin_1	131	137	PF00069	0.376
MOD_ProDKin_1	367	373	PF00069	0.454
MOD_ProDKin_1	531	537	PF00069	0.331
MOD_ProDKin_1	581	587	PF00069	0.404
MOD_ProDKin_1	70	76	PF00069	0.506
MOD_ProDKin_1	84	90	PF00069	0.564
MOD_SUMO_rev_2	424	432	PF00179	0.545
TRG_DiLeu_BaLyEn_6	324	329	PF01217	0.489
TRG_ENDOCYTIC_2	542	545	PF00928	0.364
TRG_ENDOCYTIC_2	91	94	PF00928	0.248
TRG_ER_diArg_1	182	184	PF00400	0.355
TRG_ER_diArg_1	394	397	PF00400	0.534
TRG_ER_diArg_1	483	486	PF00400	0.355
TRG_ER_diArg_1	521	524	PF00400	0.414
TRG_Pf-PMV_PEXEL_1	229	233	PF00026	0.410
TRG_Pf-PMV_PEXEL_1	435	439	PF00026	0.538

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0V4	Leptomonas seymouri	49%	100%
A0A1X0NK59	Trypanosomatidae	38%	100%
A0A3S7WPQ8	Leishmania donovani	79%	97%
A0A422NTD5	Trypanosoma rangeli	41%	100%
A4HT56	Leishmania infantum	79%	97%
C9ZUV8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
E9AL23	Leishmania mexicana (strain MHOM/GT/2001/U1103)	78%	98%
Q4QIT1	Leishmania major	79%	100%
V5BDF2	Trypanosoma cruzi	39%	100%