Uncharacterized protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Isy1-like splicing family, putative

Species:

Leishmania braziliensis

UniProt:

A4H4U5_LEIBR

TriTrypDb:

LbrM.07.0020 , LBRM2903_070005300

Length:

269

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	11
GO:0043226	organelle	2	11
GO:0043227	membrane-bounded organelle	3	11
GO:0043229	intracellular organelle	3	11
GO:0043231	intracellular membrane-bounded organelle	4	11
GO:0110165	cellular anatomical entity	1	11
GO:0000974	Prp19 complex	2	1
GO:0005681	spliceosomal complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0071013	catalytic step 2 spliceosome	3	1
GO:0071014	post-mRNA release spliceosomal complex	4	1
GO:0071020	post-spliceosomal complex	4	1
GO:0140513	nuclear protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

A4H4U5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4U5

Function

Biological processes
Term	Name	Level	Count
GO:0000350	generation of catalytic spliceosome for second transesterification step	7	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0022607	cellular component assembly	4	12
GO:0022618	ribonucleoprotein complex assembly	6	12
GO:0043933	protein-containing complex organization	4	12
GO:0065003	protein-containing complex assembly	5	12
GO:0071826	ribonucleoprotein complex subunit organization	5	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0000389	mRNA 3'-splice site recognition	8	1
GO:0006376	mRNA splice site selection	7	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	103	105	PF00675	0.312
CLV_NRD_NRD_1	124	126	PF00675	0.292
CLV_NRD_NRD_1	142	144	PF00675	0.262
CLV_NRD_NRD_1	17	19	PF00675	0.282
CLV_NRD_NRD_1	247	249	PF00675	0.280
CLV_NRD_NRD_1	253	255	PF00675	0.294
CLV_NRD_NRD_1	29	31	PF00675	0.319
CLV_PCSK_KEX2_1	144	146	PF00082	0.346
CLV_PCSK_KEX2_1	247	249	PF00082	0.223
CLV_PCSK_KEX2_1	28	30	PF00082	0.291
CLV_PCSK_PC1ET2_1	144	146	PF00082	0.346
CLV_PCSK_PC1ET2_1	247	249	PF00082	0.254
CLV_PCSK_PC1ET2_1	28	30	PF00082	0.291
CLV_PCSK_SKI1_1	127	131	PF00082	0.337
CLV_PCSK_SKI1_1	255	259	PF00082	0.309
CLV_PCSK_SKI1_1	35	39	PF00082	0.241
CLV_PCSK_SKI1_1	55	59	PF00082	0.278
DEG_Nend_UBRbox_3	1	3	PF02207	0.543
DOC_ANK_TNKS_1	144	151	PF00023	0.498
DOC_PP1_RVXF_1	109	115	PF00149	0.563
DOC_PP1_RVXF_1	186	192	PF00149	0.556
DOC_PP1_RVXF_1	56	63	PF00149	0.493
DOC_PP2B_LxvP_1	238	241	PF13499	0.400
DOC_USP7_MATH_1	160	164	PF00917	0.563
DOC_USP7_MATH_1	223	227	PF00917	0.475
DOC_USP7_UBL2_3	211	215	PF12436	0.556
LIG_14-3-3_CanoR_1	111	115	PF00244	0.538
LIG_14-3-3_CanoR_1	125	130	PF00244	0.510
LIG_14-3-3_CanoR_1	222	232	PF00244	0.577
LIG_14-3-3_CterR_2	266	269	PF00244	0.612
LIG_Clathr_ClatBox_1	80	84	PF01394	0.501
LIG_FHA_1	223	229	PF00498	0.529
LIG_FHA_1	70	76	PF00498	0.559
LIG_PCNA_yPIPBox_3	92	102	PF02747	0.487
LIG_SH2_STAT5	185	188	PF00017	0.512
LIG_SH2_STAT5	232	235	PF00017	0.501
LIG_SH2_STAT5	59	62	PF00017	0.517
LIG_SH3_3	116	122	PF00018	0.498
LIG_SH3_3	215	221	PF00018	0.400
LIG_SH3_3	42	48	PF00018	0.487
LIG_SUMO_SIM_par_1	79	84	PF11976	0.518
LIG_TRAF2_1	241	244	PF00917	0.475
LIG_TRAF2_1	257	260	PF00917	0.470
LIG_UBA3_1	22	28	PF00899	0.501
LIG_UBA3_1	61	65	PF00899	0.517
LIG_UBA3_1	80	85	PF00899	0.400
MOD_CK2_1	159	165	PF00069	0.500
MOD_GlcNHglycan	194	197	PF01048	0.349
MOD_GSK3_1	8	15	PF00069	0.555
MOD_N-GLC_1	160	165	PF02516	0.354
MOD_N-GLC_1	216	221	PF02516	0.200
MOD_NEK2_1	192	197	PF00069	0.581
MOD_NEK2_1	228	233	PF00069	0.556
MOD_PK_1	79	85	PF00069	0.558
MOD_PKA_1	125	131	PF00069	0.400
MOD_PKA_1	18	24	PF00069	0.487
MOD_PKA_2	110	116	PF00069	0.581
MOD_PKA_2	205	211	PF00069	0.539
MOD_PKA_2	260	266	PF00069	0.523
MOD_PKB_1	123	131	PF00069	0.400
MOD_Plk_4	110	116	PF00069	0.565
MOD_Plk_4	125	131	PF00069	0.520
MOD_Plk_4	18	24	PF00069	0.476
MOD_SUMO_for_1	64	67	PF00179	0.537
MOD_SUMO_rev_2	15	21	PF00179	0.503
MOD_SUMO_rev_2	205	212	PF00179	0.512
MOD_SUMO_rev_2	231	239	PF00179	0.581
MOD_SUMO_rev_2	50	57	PF00179	0.515
TRG_DiLeu_BaEn_1	97	102	PF01217	0.487
TRG_ENDOCYTIC_2	59	62	PF00928	0.501
TRG_ER_diArg_1	122	125	PF00400	0.563
TRG_ER_diArg_1	145	148	PF00400	0.550
TRG_NLS_Bipartite_1	125	147	PF00514	0.561
TRG_NLS_MonoExtC_3	142	147	PF00514	0.561
TRG_NLS_MonoExtN_4	122	129	PF00514	0.581
TRG_NLS_MonoExtN_4	143	148	PF00514	0.509
TRG_Pf-PMV_PEXEL_1	134	138	PF00026	0.287
TRG_Pf-PMV_PEXEL_1	256	260	PF00026	0.328

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PAF4	Leptomonas seymouri	65%	100%
A0A0S4IZ16	Bodo saltans	37%	100%
A0A1X0NL28	Trypanosomatidae	44%	100%
A0A3R7KL93	Trypanosoma rangeli	43%	100%
A0A3S5H5V5	Leishmania donovani	79%	100%
A4HT23	Leishmania infantum	80%	92%
C9ZUW9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	97%
E9AL11	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	83%
Q4QIU3	Leishmania major	82%	100%
V5B8Q7	Trypanosoma cruzi	43%	100%