Bifunctional dihydrofolate reductase-thymidylate synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

dihydrofolate reductase-thymidylate synthase

Species:

Leishmania braziliensis

UniProt:

A4H4P8_LEIBR

TriTrypDb:

LbrM.06.0830 , LBRM2903_060015100

Length:

521

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H4P8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4P8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	6
GO:0006220	pyrimidine nucleotide metabolic process	5	6
GO:0006221	pyrimidine nucleotide biosynthetic process	6	6
GO:0006231	dTMP biosynthetic process	8	6
GO:0006575	cellular modified amino acid metabolic process	3	6
GO:0006725	cellular aromatic compound metabolic process	3	6
GO:0006730	one-carbon metabolic process	3	6
GO:0006753	nucleoside phosphate metabolic process	4	6
GO:0006760	folic acid-containing compound metabolic process	4	6
GO:0006793	phosphorus metabolic process	3	6
GO:0006796	phosphate-containing compound metabolic process	4	6
GO:0006807	nitrogen compound metabolic process	2	6
GO:0008152	metabolic process	1	6
GO:0009058	biosynthetic process	2	6
GO:0009117	nucleotide metabolic process	5	6
GO:0009123	nucleoside monophosphate metabolic process	5	6
GO:0009124	nucleoside monophosphate biosynthetic process	6	6
GO:0009129	pyrimidine nucleoside monophosphate metabolic process	6	6
GO:0009130	pyrimidine nucleoside monophosphate biosynthetic process	7	6
GO:0009157	deoxyribonucleoside monophosphate biosynthetic process	7	6
GO:0009162	deoxyribonucleoside monophosphate metabolic process	6	6
GO:0009165	nucleotide biosynthetic process	6	6
GO:0009176	pyrimidine deoxyribonucleoside monophosphate metabolic process	7	6
GO:0009177	pyrimidine deoxyribonucleoside monophosphate biosynthetic process	8	6
GO:0009219	pyrimidine deoxyribonucleotide metabolic process	6	6
GO:0009221	pyrimidine deoxyribonucleotide biosynthetic process	7	6
GO:0009262	deoxyribonucleotide metabolic process	4	6
GO:0009263	deoxyribonucleotide biosynthetic process	5	6
GO:0009265	2'-deoxyribonucleotide biosynthetic process	6	6
GO:0009394	2'-deoxyribonucleotide metabolic process	5	6
GO:0009396	folic acid-containing compound biosynthetic process	5	6
GO:0009987	cellular process	1	6
GO:0018130	heterocycle biosynthetic process	4	6
GO:0019438	aromatic compound biosynthetic process	4	6
GO:0019637	organophosphate metabolic process	3	6
GO:0019692	deoxyribose phosphate metabolic process	4	6
GO:0032259	methylation	2	6
GO:0034641	cellular nitrogen compound metabolic process	3	6
GO:0034654	nucleobase-containing compound biosynthetic process	4	6
GO:0042398	cellular modified amino acid biosynthetic process	4	6
GO:0042558	pteridine-containing compound metabolic process	4	6
GO:0042559	pteridine-containing compound biosynthetic process	5	6
GO:0044237	cellular metabolic process	2	6
GO:0044238	primary metabolic process	2	6
GO:0044249	cellular biosynthetic process	3	6
GO:0044271	cellular nitrogen compound biosynthetic process	4	6
GO:0044281	small molecule metabolic process	2	6
GO:0046073	dTMP metabolic process	7	6
GO:0046385	deoxyribose phosphate biosynthetic process	5	6
GO:0046483	heterocycle metabolic process	3	6
GO:0046653	tetrahydrofolate metabolic process	5	6
GO:0046654	tetrahydrofolate biosynthetic process	6	6
GO:0055086	nucleobase-containing small molecule metabolic process	3	6
GO:0071704	organic substance metabolic process	2	6
GO:0072527	pyrimidine-containing compound metabolic process	4	6
GO:0072528	pyrimidine-containing compound biosynthetic process	5	6
GO:0090407	organophosphate biosynthetic process	4	6
GO:1901135	carbohydrate derivative metabolic process	3	6
GO:1901137	carbohydrate derivative biosynthetic process	4	6
GO:1901293	nucleoside phosphate biosynthetic process	5	6
GO:1901360	organic cyclic compound metabolic process	3	6
GO:1901362	organic cyclic compound biosynthetic process	4	6
GO:1901564	organonitrogen compound metabolic process	3	6
GO:1901566	organonitrogen compound biosynthetic process	4	6
GO:1901576	organic substance biosynthetic process	3	6

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	6
GO:0004146	dihydrofolate reductase activity	5	6
GO:0004799	thymidylate synthase activity	6	6
GO:0008168	methyltransferase activity	4	6
GO:0016491	oxidoreductase activity	2	6
GO:0016645	oxidoreductase activity, acting on the CH-NH group of donors	3	6
GO:0016646	oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor	4	6
GO:0016740	transferase activity	2	6
GO:0016741	transferase activity, transferring one-carbon groups	3	6
GO:0042083	5,10-methylenetetrahydrofolate-dependent methyltransferase activity	5	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	271	275	PF00656	0.490
CLV_C14_Caspase3-7	322	326	PF00656	0.490
CLV_C14_Caspase3-7	459	463	PF00656	0.490
CLV_NRD_NRD_1	225	227	PF00675	0.231
CLV_NRD_NRD_1	381	383	PF00675	0.290
CLV_PCSK_KEX2_1	119	121	PF00082	0.257
CLV_PCSK_KEX2_1	283	285	PF00082	0.290
CLV_PCSK_KEX2_1	381	383	PF00082	0.290
CLV_PCSK_KEX2_1	490	492	PF00082	0.193
CLV_PCSK_KEX2_1	73	75	PF00082	0.290
CLV_PCSK_PC1ET2_1	119	121	PF00082	0.257
CLV_PCSK_PC1ET2_1	283	285	PF00082	0.290
CLV_PCSK_PC1ET2_1	490	492	PF00082	0.193
CLV_PCSK_PC1ET2_1	73	75	PF00082	0.290
CLV_PCSK_SKI1_1	244	248	PF00082	0.316
CLV_PCSK_SKI1_1	283	287	PF00082	0.334
CLV_PCSK_SKI1_1	321	325	PF00082	0.310
CLV_PCSK_SKI1_1	327	331	PF00082	0.264
CLV_PCSK_SKI1_1	381	385	PF00082	0.290
CLV_PCSK_SKI1_1	493	497	PF00082	0.257
CLV_PCSK_SKI1_1	92	96	PF00082	0.290
DEG_APCC_DBOX_1	235	243	PF00400	0.341
DOC_CYCLIN_yCln2_LP_2	429	435	PF00134	0.490
DOC_MAPK_FxFP_2	19	22	PF00069	0.389
DOC_MAPK_gen_1	252	260	PF00069	0.393
DOC_MAPK_gen_1	7	14	PF00069	0.323
DOC_MAPK_gen_1	92	102	PF00069	0.436
DOC_MAPK_MEF2A_6	25	33	PF00069	0.341
DOC_MAPK_MEF2A_6	423	431	PF00069	0.490
DOC_MAPK_MEF2A_6	95	102	PF00069	0.427
DOC_PP1_RVXF_1	281	288	PF00149	0.490
DOC_PP2B_LxvP_1	429	432	PF13499	0.490
DOC_PP4_FxxP_1	19	22	PF00568	0.289
DOC_PP4_FxxP_1	8	11	PF00568	0.410
DOC_SPAK_OSR1_1	7	11	PF12202	0.401
DOC_USP7_MATH_1	145	149	PF00917	0.457
DOC_USP7_MATH_1	20	24	PF00917	0.382
DOC_WW_Pin1_4	477	482	PF00397	0.396
LIG_14-3-3_CanoR_1	181	187	PF00244	0.490
LIG_14-3-3_CanoR_1	213	221	PF00244	0.490
LIG_14-3-3_CanoR_1	269	273	PF00244	0.490
LIG_14-3-3_CanoR_1	327	335	PF00244	0.496
LIG_14-3-3_CanoR_1	382	390	PF00244	0.490
LIG_14-3-3_CanoR_1	81	85	PF00244	0.490
LIG_Actin_WH2_2	436	454	PF00022	0.490
LIG_BIR_II_1	1	5	PF00653	0.466
LIG_BRCT_BRCA1_1	17	21	PF00533	0.305
LIG_BRCT_BRCA1_1	319	323	PF00533	0.490
LIG_CtBP_PxDLS_1	50	54	PF00389	0.490
LIG_EVH1_1	193	197	PF00568	0.490
LIG_FHA_1	216	222	PF00498	0.393
LIG_FHA_1	280	286	PF00498	0.490
LIG_FHA_1	371	377	PF00498	0.393
LIG_FHA_1	58	64	PF00498	0.490
LIG_FHA_2	105	111	PF00498	0.490
LIG_FHA_2	183	189	PF00498	0.407
LIG_FHA_2	457	463	PF00498	0.490
LIG_LIR_Apic_2	17	22	PF02991	0.420
LIG_LIR_Apic_2	190	196	PF02991	0.547
LIG_LIR_Apic_2	44	50	PF02991	0.490
LIG_LIR_Gen_1	235	246	PF02991	0.341
LIG_LIR_Gen_1	437	446	PF02991	0.490
LIG_LIR_Gen_1	83	91	PF02991	0.490
LIG_LIR_LC3C_4	126	129	PF02991	0.393
LIG_LIR_Nem_3	18	24	PF02991	0.403
LIG_LIR_Nem_3	190	195	PF02991	0.502
LIG_LIR_Nem_3	235	241	PF02991	0.341
LIG_LIR_Nem_3	437	441	PF02991	0.490
LIG_LIR_Nem_3	508	512	PF02991	0.469
LIG_LIR_Nem_3	83	87	PF02991	0.490
LIG_LYPXL_yS_3	512	515	PF13949	0.393
LIG_REV1ctd_RIR_1	53	61	PF16727	0.490
LIG_SH2_CRK	438	442	PF00017	0.490
LIG_SH2_CRK	91	95	PF00017	0.393
LIG_SH2_GRB2like	408	411	PF00017	0.490
LIG_SH2_NCK_1	183	187	PF00017	0.502
LIG_SH2_SRC	228	231	PF00017	0.271
LIG_SH2_STAP1	162	166	PF00017	0.490
LIG_SH2_STAT3	341	344	PF00017	0.490
LIG_SH2_STAT5	154	157	PF00017	0.451
LIG_SH2_STAT5	178	181	PF00017	0.490
LIG_SH2_STAT5	228	231	PF00017	0.406
LIG_SH2_STAT5	238	241	PF00017	0.425
LIG_SH2_STAT5	408	411	PF00017	0.490
LIG_SH3_1	191	197	PF00018	0.490
LIG_SH3_2	511	516	PF14604	0.441
LIG_SH3_3	191	197	PF00018	0.516
LIG_SH3_3	508	514	PF00018	0.485
LIG_SUMO_SIM_anti_2	126	131	PF11976	0.490
LIG_SUMO_SIM_par_1	28	35	PF11976	0.490
LIG_TYR_ITIM	89	94	PF00017	0.393
LIG_UBA3_1	111	119	PF00899	0.457
LIG_UBA3_1	241	247	PF00899	0.490
LIG_UBA3_1	440	447	PF00899	0.490
MOD_CK1_1	104	110	PF00069	0.490
MOD_CK1_1	149	155	PF00069	0.490
MOD_CK2_1	104	110	PF00069	0.490
MOD_CK2_1	145	151	PF00069	0.490
MOD_CK2_1	182	188	PF00069	0.393
MOD_CK2_1	366	372	PF00069	0.483
MOD_CK2_1	499	505	PF00069	0.473
MOD_Cter_Amidation	117	120	PF01082	0.241
MOD_GSK3_1	100	107	PF00069	0.490
MOD_GSK3_1	145	152	PF00069	0.490
MOD_GSK3_1	160	167	PF00069	0.395
MOD_GSK3_1	323	330	PF00069	0.505
MOD_GSK3_1	366	373	PF00069	0.441
MOD_N-GLC_1	317	322	PF02516	0.290
MOD_NEK2_1	207	212	PF00069	0.490
MOD_NEK2_1	268	273	PF00069	0.573
MOD_NEK2_1	279	284	PF00069	0.463
MOD_NEK2_1	323	328	PF00069	0.490
MOD_NEK2_1	383	388	PF00069	0.490
MOD_NEK2_1	456	461	PF00069	0.490
MOD_NEK2_2	259	264	PF00069	0.490
MOD_OFUCOSY	418	425	PF10250	0.290
MOD_PIKK_1	207	213	PF00454	0.490
MOD_PK_1	25	31	PF00069	0.341
MOD_PKA_2	268	274	PF00069	0.490
MOD_PKA_2	80	86	PF00069	0.490
MOD_PKB_1	213	221	PF00069	0.393
MOD_Plk_1	187	193	PF00069	0.393
MOD_Plk_1	414	420	PF00069	0.490
MOD_Plk_1	57	63	PF00069	0.480
MOD_Plk_4	259	265	PF00069	0.409
MOD_Plk_4	366	372	PF00069	0.393
MOD_Plk_4	439	445	PF00069	0.490
MOD_Plk_4	483	489	PF00069	0.490
MOD_Plk_4	58	64	PF00069	0.478
MOD_ProDKin_1	477	483	PF00069	0.396
MOD_SUMO_for_1	489	492	PF00179	0.490
MOD_SUMO_for_1	515	518	PF00179	0.473
MOD_SUMO_rev_2	35	45	PF00179	0.393
TRG_DiLeu_BaEn_1	136	141	PF01217	0.473
TRG_DiLeu_BaEn_3	292	298	PF01217	0.490
TRG_DiLeu_BaEn_4	234	240	PF01217	0.341
TRG_DiLeu_BaLyEn_6	275	280	PF01217	0.490
TRG_DiLeu_BaLyEn_6	379	384	PF01217	0.490
TRG_ENDOCYTIC_2	238	241	PF00928	0.490
TRG_ENDOCYTIC_2	352	355	PF00928	0.495
TRG_ENDOCYTIC_2	438	441	PF00928	0.490
TRG_ENDOCYTIC_2	512	515	PF00928	0.488
TRG_ENDOCYTIC_2	91	94	PF00928	0.393
TRG_ER_diArg_1	381	383	PF00400	0.490

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJX9	Leptomonas seymouri	76%	98%
A0A0S4JJI0	Bodo saltans	59%	100%
A0A1X0NKM2	Trypanosomatidae	67%	100%
A0A3R7P0P8	Trypanosoma rangeli	65%	100%
A0A3S5H5T0	Leishmania donovani	88%	100%
C9ZTK8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	99%
E9AKW4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
O81395	Zea mays	45%	100%
P07382	Leishmania major	87%	100%
P16126	Leishmania amazonensis	87%	100%
P20712	Plasmodium chabaudi	39%	89%
P45350	Daucus carota	44%	99%
P51820	Glycine max	44%	98%
Q05762	Arabidopsis thaliana	45%	100%
Q05763	Arabidopsis thaliana	46%	92%
Q23695	Crithidia fasciculata	80%	100%
Q27783	Trypanosoma brucei brucei	61%	99%
Q27793	Trypanosoma cruzi	66%	100%
Q27828	Paramecium tetraurelia	42%	100%
Q2QRX6	Oryza sativa subsp. japonica	46%	100%
Q8MQV3	Leishmania infantum	88%	100%
V5DPL1	Trypanosoma cruzi	66%	100%