Putative glutamine synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glutamine synthetase, putative

Species:

Leishmania braziliensis

UniProt:

A4H4K2_LEIBR

TriTrypDb:

LbrM.06.0350 , LBRM2903_060009200

Length:

379

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	yes	yes: 1
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 4
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H4K2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4K2

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	13
GO:0006520	amino acid metabolic process	3	13
GO:0006541	glutamine metabolic process	6	13
GO:0006542	glutamine biosynthetic process	7	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0008652	amino acid biosynthetic process	4	13
GO:0009058	biosynthetic process	2	13
GO:0009064	glutamine family amino acid metabolic process	5	13
GO:0009084	glutamine family amino acid biosynthetic process	6	13
GO:0009987	cellular process	1	13
GO:0016053	organic acid biosynthetic process	4	13
GO:0019752	carboxylic acid metabolic process	5	13
GO:0043436	oxoacid metabolic process	4	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044249	cellular biosynthetic process	3	13
GO:0044281	small molecule metabolic process	2	13
GO:0044283	small molecule biosynthetic process	3	13
GO:0046394	carboxylic acid biosynthetic process	5	13
GO:0071704	organic substance metabolic process	2	13
GO:1901564	organonitrogen compound metabolic process	3	13
GO:1901566	organonitrogen compound biosynthetic process	4	13
GO:1901576	organic substance biosynthetic process	3	13
GO:1901605	alpha-amino acid metabolic process	4	13
GO:1901607	alpha-amino acid biosynthetic process	5	13

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0004356	glutamate-ammonia ligase activity	6	13
GO:0016211	ammonia ligase activity	5	13
GO:0016874	ligase activity	2	13
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	13
GO:0016880	acid-ammonia (or amide) ligase activity	4	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	139	143	PF00656	0.474
CLV_C14_Caspase3-7	17	21	PF00656	0.568
CLV_C14_Caspase3-7	231	235	PF00656	0.616
CLV_NRD_NRD_1	171	173	PF00675	0.358
CLV_NRD_NRD_1	276	278	PF00675	0.318
CLV_NRD_NRD_1	335	337	PF00675	0.353
CLV_PCSK_KEX2_1	335	337	PF00082	0.340
CLV_PCSK_SKI1_1	221	225	PF00082	0.348
CLV_PCSK_SKI1_1	375	379	PF00082	0.703
CLV_PCSK_SKI1_1	44	48	PF00082	0.357
DEG_APCC_DBOX_1	220	228	PF00400	0.557
DOC_CKS1_1	357	362	PF01111	0.584
DOC_CYCLIN_yCln2_LP_2	73	79	PF00134	0.581
DOC_MAPK_DCC_7	86	96	PF00069	0.557
DOC_MAPK_gen_1	26	34	PF00069	0.568
DOC_PP1_RVXF_1	280	287	PF00149	0.581
DOC_PP2B_PxIxI_1	91	97	PF00149	0.557
DOC_PP4_FxxP_1	153	156	PF00568	0.566
DOC_PP4_FxxP_1	286	289	PF00568	0.578
DOC_USP7_MATH_1	323	327	PF00917	0.643
DOC_USP7_MATH_1	85	89	PF00917	0.597
DOC_USP7_UBL2_3	278	282	PF12436	0.616
DOC_WW_Pin1_4	194	199	PF00397	0.620
DOC_WW_Pin1_4	356	361	PF00397	0.547
DOC_WW_Pin1_4	363	368	PF00397	0.521
LIG_14-3-3_CanoR_1	10	14	PF00244	0.548
LIG_14-3-3_CanoR_1	275	281	PF00244	0.581
LIG_14-3-3_CanoR_1	314	321	PF00244	0.597
LIG_14-3-3_CanoR_1	349	357	PF00244	0.548
LIG_Actin_WH2_2	262	279	PF00022	0.475
LIG_BIR_II_1	1	5	PF00653	0.622
LIG_BIR_III_2	340	344	PF00653	0.568
LIG_BRCT_BRCA1_1	347	351	PF00533	0.568
LIG_FHA_1	29	35	PF00498	0.548
LIG_FHA_1	299	305	PF00498	0.587
LIG_FHA_1	345	351	PF00498	0.614
LIG_FHA_1	357	363	PF00498	0.396
LIG_FHA_1	6	12	PF00498	0.575
LIG_FHA_1	68	74	PF00498	0.562
LIG_FHA_2	137	143	PF00498	0.474
LIG_FHA_2	190	196	PF00498	0.557
LIG_FHA_2	258	264	PF00498	0.568
LIG_IRF3_LxIS_1	183	190	PF10401	0.616
LIG_LIR_Apic_2	283	289	PF02991	0.578
LIG_LIR_Gen_1	12	21	PF02991	0.547
LIG_LIR_Nem_3	12	16	PF02991	0.547
LIG_LIR_Nem_3	126	132	PF02991	0.486
LIG_NRBOX	223	229	PF00104	0.639
LIG_PCNA_yPIPBox_3	216	226	PF02747	0.557
LIG_Pex14_1	170	174	PF04695	0.654
LIG_PTB_Apo_2	46	53	PF02174	0.654
LIG_SH2_PTP2	13	16	PF00017	0.548
LIG_SH2_SRC	101	104	PF00017	0.633
LIG_SH2_STAP1	179	183	PF00017	0.545
LIG_SH2_STAT3	33	36	PF00017	0.639
LIG_SH2_STAT5	101	104	PF00017	0.616
LIG_SH2_STAT5	13	16	PF00017	0.548
LIG_SH2_STAT5	136	139	PF00017	0.585
LIG_SH2_STAT5	161	164	PF00017	0.547
LIG_SH2_STAT5	272	275	PF00017	0.548
LIG_SH2_STAT5	33	36	PF00017	0.548
LIG_SH2_STAT5	344	347	PF00017	0.548
LIG_SUMO_SIM_anti_2	361	366	PF11976	0.491
LIG_SUMO_SIM_par_1	93	99	PF11976	0.557
LIG_SxIP_EBH_1	86	97	PF03271	0.639
LIG_TYR_ITIM	11	16	PF00017	0.557
MOD_CK1_1	109	115	PF00069	0.669
MOD_CK1_1	163	169	PF00069	0.639
MOD_CK1_1	256	262	PF00069	0.597
MOD_CK1_1	302	308	PF00069	0.639
MOD_CK2_1	189	195	PF00069	0.557
MOD_CK2_1	257	263	PF00069	0.559
MOD_Cter_Amidation	170	173	PF01082	0.357
MOD_GlcNHglycan	103	106	PF01048	0.545
MOD_GlcNHglycan	16	19	PF01048	0.377
MOD_GlcNHglycan	189	192	PF01048	0.357
MOD_GlcNHglycan	248	251	PF01048	0.348
MOD_GlcNHglycan	306	310	PF01048	0.409
MOD_GSK3_1	1	8	PF00069	0.597
MOD_GSK3_1	101	108	PF00069	0.619
MOD_GSK3_1	161	168	PF00069	0.630
MOD_GSK3_1	24	31	PF00069	0.539
MOD_GSK3_1	246	253	PF00069	0.548
MOD_GSK3_1	298	305	PF00069	0.578
MOD_GSK3_1	310	317	PF00069	0.491
MOD_N-GLC_1	187	192	PF02516	0.368
MOD_N-GLC_1	246	251	PF02516	0.343
MOD_N-GLC_1	253	258	PF02516	0.344
MOD_N-GLC_1	5	10	PF02516	0.569
MOD_NEK2_1	1	6	PF00069	0.688
MOD_NEK2_1	187	192	PF00069	0.570
MOD_NEK2_1	276	281	PF00069	0.539
MOD_NEK2_1	32	37	PF00069	0.474
MOD_NEK2_1	345	350	PF00069	0.557
MOD_NEK2_1	57	62	PF00069	0.544
MOD_PIKK_1	32	38	PF00454	0.654
MOD_PK_1	314	320	PF00069	0.548
MOD_PKA_2	116	122	PF00069	0.613
MOD_PKA_2	276	282	PF00069	0.618
MOD_PKA_2	293	299	PF00069	0.442
MOD_PKA_2	313	319	PF00069	0.418
MOD_PKA_2	85	91	PF00069	0.557
MOD_PKA_2	9	15	PF00069	0.400
MOD_Plk_1	28	34	PF00069	0.548
MOD_Plk_1	5	11	PF00069	0.562
MOD_Plk_2-3	358	364	PF00069	0.657
MOD_Plk_4	9	15	PF00069	0.400
MOD_ProDKin_1	194	200	PF00069	0.620
MOD_ProDKin_1	356	362	PF00069	0.542
MOD_ProDKin_1	363	369	PF00069	0.516
TRG_DiLeu_BaEn_2	132	138	PF01217	0.557
TRG_DiLeu_BaLyEn_6	223	228	PF01217	0.637
TRG_ENDOCYTIC_2	13	16	PF00928	0.547
TRG_ER_diArg_1	335	337	PF00400	0.548
TRG_Pf-PMV_PEXEL_1	226	230	PF00026	0.348

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6V5	Leptomonas seymouri	84%	100%
A0A0S4IX33	Bodo saltans	62%	100%
A0A1X0NKP6	Trypanosomatidae	75%	100%
A0A1X0NL55	Trypanosomatidae	75%	99%
A0A3R7R8D5	Trypanosoma rangeli	74%	99%
A0A3S5H5P8	Leishmania donovani	92%	100%
A0R083	Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)	23%	85%
A4HSS8	Leishmania infantum	92%	78%
C9ZTD4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	69%	90%
E9AKR5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
O00088	Agaricus bisporus	46%	100%
O04867	Alnus glutinosa	45%	100%
O22504	Daucus carota	45%	100%
O22506	Daucus carota	46%	88%
O82560	Glycine max	46%	100%
P00965	Phaseolus vulgaris	46%	100%
P04078	Medicago sativa	44%	100%
P04770	Phaseolus vulgaris	44%	100%
P04771	Phaseolus vulgaris	44%	100%
P04772	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	39%	100%
P04773	Cricetulus griseus	43%	100%
P07694	Pisum sativum	44%	100%
P08281	Pisum sativum	44%	88%
P08282	Pisum sativum	45%	100%
P09606	Rattus norvegicus	45%	100%
P0CN84	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	46%	100%
P0CN85	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	46%	100%
P12424	Nicotiana plumbaginifolia	45%	100%
P13564	Hordeum vulgare	45%	87%
P14636	Lupinus angustifolius	46%	100%
P14654	Oryza sativa subsp. japonica	44%	100%
P14655	Oryza sativa subsp. japonica	45%	89%
P14656	Oryza sativa subsp. japonica	45%	100%
P15102	Phaseolus vulgaris	46%	88%
P15103	Bos taurus	45%	100%
P15104	Homo sapiens	46%	100%
P15105	Mus musculus	46%	100%
P16580	Gallus gallus	46%	100%
P19432	Streptomyces viridochromogenes	44%	100%
P20477	Drosophila melanogaster	42%	95%
P20478	Drosophila melanogaster	46%	100%
P20805	Frankia alni	41%	100%
P22878	Streptomyces hygroscopicus	41%	100%
P23712	Lactuca sativa	46%	100%
P24099	Glycine max	44%	100%
P25462	Zea mays	45%	90%
P32288	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	46%	100%
P32289	Vigna aconitifolia	44%	100%
P34497	Caenorhabditis elegans	46%	100%
P36205	Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)	26%	86%
P38559	Zea mays	46%	100%
P38560	Zea mays	45%	100%
P38561	Zea mays	46%	100%
P38562	Zea mays	45%	100%
P38563	Zea mays	45%	100%
P41320	Squalus acanthias	43%	94%
P45626	Rhizobium meliloti	39%	100%
P46410	Sus scrofa	45%	100%
P51118	Vitis vinifera	45%	100%
P51119	Vitis vinifera	44%	100%
P51121	Xenopus laevis	46%	97%
P52782	Lupinus luteus	46%	100%
P52783	Pinus sylvestris	45%	100%
Q02154	Rhizobium leguminosarum bv. phaseoli	39%	100%
Q04831	Panulirus argus	47%	100%
Q06378	Hordeum vulgare	44%	100%
Q09179	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	46%	100%
Q12613	Colletotrichum gloeosporioides	47%	100%
Q42624	Brassica napus	47%	89%
Q42688	Chlamydomonas reinhardtii	44%	99%
Q42689	Chlamydomonas reinhardtii	42%	100%
Q42899	Lotus japonicus	46%	100%
Q43066	Pisum sativum	44%	100%
Q43127	Arabidopsis thaliana	47%	88%
Q43785	Medicago sativa	43%	100%
Q4QJ42	Leishmania major	92%	100%
Q4R7U3	Macaca fascicularis	46%	100%
Q4W8D0	Oryza sativa subsp. japonica	45%	100%
Q56WN1	Arabidopsis thaliana	44%	100%
Q5SIP0	Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)	28%	85%
Q5UR44	Acanthamoeba polyphaga mimivirus	46%	100%
Q6B4U7	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	45%	100%
Q6C3E0	Yarrowia lipolytica (strain CLIB 122 / E 150)	44%	100%
Q6FMT6	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	46%	100%
Q75BT9	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	46%	100%
Q86ZF9	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	47%	100%
Q86ZU6	Tuber borchii	46%	100%
Q874T6	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	46%	100%
Q8GXW5	Arabidopsis thaliana	46%	100%
Q8HZM5	Canis lupus familiaris	45%	100%
Q8J1R3	Suillus bovinus	43%	100%
Q8LCE1	Arabidopsis thaliana	44%	100%
Q8X169	Amanita muscaria	43%	100%
Q96UV5	Hebeloma cylindrosporum	45%	100%
Q96V52	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	46%	100%
Q9C2U9	Gibberella fujikuroi	46%	100%
Q9CDL9	Lactococcus lactis subsp. lactis (strain IL1403)	25%	85%
Q9FMD9	Arabidopsis thaliana	45%	100%
Q9LVI8	Arabidopsis thaliana	46%	100%
Q9QY94	Acomys cahirinus	46%	100%
Q9UUN6	Fusarium solani subsp. phaseoli	47%	100%
Q9XQ94	Medicago sativa	45%	89%
V5CHQ8	Trypanosoma cruzi	76%	99%