NAD(P)-dependent steroid dehydrogenase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NAD(P)-dependent steroid dehydrogenase protein, putative

Species:

Leishmania braziliensis

UniProt:

A4H4K0_LEIBR

TriTrypDb:

LbrM.06.0330 , LBRM2903_060009000

Length:

363

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

A4H4K0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4K0

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	12
GO:0006694	steroid biosynthetic process	5	12
GO:0008152	metabolic process	1	12
GO:0008202	steroid metabolic process	4	12
GO:0008610	lipid biosynthetic process	4	12
GO:0009058	biosynthetic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity	5	12
GO:0016229	steroid dehydrogenase activity	3	12
GO:0016491	oxidoreductase activity	2	12
GO:0016614	oxidoreductase activity, acting on CH-OH group of donors	3	12
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	4	12
GO:0033764	steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	4	12
GO:0000252	C-3 sterol dehydrogenase (C-4 sterol decarboxylase) activity	5	1
GO:0047012	sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating) activity	5	1
GO:0103066	4alpha-carboxy-4beta-methyl-5alpha-cholesta-8-en-3beta-ol:NAD(P)+ 3-oxidoreductase (decarboxylating) activity	5	1
GO:0103067	4alpha-carboxy-5alpha-cholesta-8-en-3beta-ol:NAD(P)+ 3-dehydrogenase (decarboxylating) activity	5	1
GO:0102294	cholesterol dehydrogenase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	195	199	PF00656	0.454
CLV_NRD_NRD_1	359	361	PF00675	0.683
CLV_PCSK_KEX2_1	70	72	PF00082	0.340
CLV_PCSK_PC1ET2_1	70	72	PF00082	0.178
CLV_PCSK_SKI1_1	91	95	PF00082	0.315
DEG_MDM2_SWIB_1	317	325	PF02201	0.281
DEG_Nend_Nbox_1	1	3	PF02207	0.399
DOC_ANK_TNKS_1	108	115	PF00023	0.515
DOC_PP1_RVXF_1	189	195	PF00149	0.454
DOC_PP1_RVXF_1	7	14	PF00149	0.454
DOC_PP2B_LxvP_1	168	171	PF13499	0.454
DOC_PP4_FxxP_1	107	110	PF00568	0.454
DOC_SPAK_OSR1_1	220	224	PF12202	0.540
DOC_USP7_UBL2_3	209	213	PF12436	0.540
DOC_USP7_UBL2_3	241	245	PF12436	0.484
DOC_WW_Pin1_4	212	217	PF00397	0.454
DOC_WW_Pin1_4	94	99	PF00397	0.450
LIG_14-3-3_CanoR_1	191	200	PF00244	0.448
LIG_14-3-3_CanoR_1	281	285	PF00244	0.271
LIG_APCC_ABBA_1	129	134	PF00400	0.393
LIG_APCC_ABBA_1	314	319	PF00400	0.325
LIG_BRCT_BRCA1_1	98	102	PF00533	0.465
LIG_deltaCOP1_diTrp_1	319	328	PF00928	0.282
LIG_EH1_1	86	94	PF00400	0.515
LIG_FHA_1	177	183	PF00498	0.453
LIG_FHA_1	281	287	PF00498	0.384
LIG_FHA_1	75	81	PF00498	0.454
LIG_FHA_2	114	120	PF00498	0.445
LIG_FHA_2	133	139	PF00498	0.436
LIG_FHA_2	193	199	PF00498	0.454
LIG_FHA_2	223	229	PF00498	0.495
LIG_LIR_Apic_2	105	110	PF02991	0.479
LIG_LIR_Gen_1	126	133	PF02991	0.463
LIG_LIR_Gen_1	38	47	PF02991	0.479
LIG_LIR_Nem_3	148	154	PF02991	0.515
LIG_LIR_Nem_3	308	314	PF02991	0.292
LIG_LIR_Nem_3	324	330	PF02991	0.264
LIG_LIR_Nem_3	38	43	PF02991	0.474
LIG_MAD2	9	17	PF02301	0.454
LIG_NRP_CendR_1	360	363	PF00754	0.601
LIG_Pex14_1	332	336	PF04695	0.286
LIG_Pex14_2	317	321	PF04695	0.281
LIG_Pex14_2	328	332	PF04695	0.296
LIG_SH2_CRK	69	73	PF00017	0.515
LIG_SH2_NCK_1	40	44	PF00017	0.479
LIG_SH2_STAT5	200	203	PF00017	0.524
LIG_SH2_STAT5	217	220	PF00017	0.343
LIG_SH2_STAT5	222	225	PF00017	0.439
LIG_SH2_STAT5	236	239	PF00017	0.474
LIG_SH2_STAT5	267	270	PF00017	0.322
LIG_SH2_STAT5	32	35	PF00017	0.465
LIG_SH3_3	118	124	PF00018	0.444
LIG_SH3_3	158	164	PF00018	0.447
LIG_SH3_3	310	316	PF00018	0.326
LIG_SH3_3	59	65	PF00018	0.454
LIG_TYR_ITSM	36	43	PF00017	0.515
LIG_UBA3_1	329	338	PF00899	0.234
MOD_CDK_SPxxK_3	94	101	PF00069	0.454
MOD_CK1_1	254	260	PF00069	0.520
MOD_CK1_1	348	354	PF00069	0.447
MOD_CK1_1	41	47	PF00069	0.559
MOD_CK1_1	96	102	PF00069	0.454
MOD_CK2_1	113	119	PF00069	0.449
MOD_CK2_1	132	138	PF00069	0.393
MOD_CK2_1	226	232	PF00069	0.454
MOD_GlcNHglycan	348	351	PF01048	0.682
MOD_GlcNHglycan	42	46	PF01048	0.359
MOD_GSK3_1	222	229	PF00069	0.454
MOD_GSK3_1	92	99	PF00069	0.454
MOD_N-GLC_1	321	326	PF02516	0.598
MOD_N-GLC_2	199	201	PF02516	0.315
MOD_NEK2_1	102	107	PF00069	0.454
MOD_NEK2_1	279	284	PF00069	0.483
MOD_NEK2_1	292	297	PF00069	0.235
MOD_NEK2_1	92	97	PF00069	0.454
MOD_NEK2_2	35	40	PF00069	0.378
MOD_PIKK_1	348	354	PF00454	0.396
MOD_PKA_2	143	149	PF00069	0.551
MOD_PKA_2	280	286	PF00069	0.262
MOD_Plk_1	321	327	PF00069	0.356
MOD_Plk_1	339	345	PF00069	0.289
MOD_Plk_1	35	41	PF00069	0.538
MOD_Plk_2-3	113	119	PF00069	0.454
MOD_Plk_4	102	108	PF00069	0.468
MOD_Plk_4	251	257	PF00069	0.539
MOD_ProDKin_1	212	218	PF00069	0.454
MOD_ProDKin_1	94	100	PF00069	0.450
TRG_ENDOCYTIC_2	128	131	PF00928	0.465
TRG_ENDOCYTIC_2	236	239	PF00928	0.474
TRG_ENDOCYTIC_2	40	43	PF00928	0.465
TRG_ENDOCYTIC_2	69	72	PF00928	0.454

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6H3	Leptomonas seymouri	88%	89%
A0A0S4J083	Bodo saltans	67%	89%
A0A1X0NKS1	Trypanosomatidae	73%	89%
A0A3R7NBA3	Trypanosoma rangeli	74%	89%
A0A3S5H5P6	Leishmania donovani	93%	89%
A4HSS6	Leishmania infantum	93%	89%
A8DZE7	Danio rerio	26%	94%
B2FI29	Stenotrophomonas maltophilia (strain K279a)	28%	100%
C9ZTC5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	71%	90%
E9AKR3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
O43050	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	100%
O57245	Vaccinia virus (strain Ankara)	22%	100%
P21097	Vaccinia virus (strain Copenhagen)	22%	100%
P22071	Rattus norvegicus	25%	97%
P26150	Mus musculus	24%	97%
P26670	Vaccinia virus (strain Western Reserve)	22%	100%
P53199	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	100%
P75821	Escherichia coli (strain K12)	23%	100%
P9WQP6	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	25%	98%
P9WQP7	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	25%	98%
Q0IH73	Xenopus laevis	26%	94%
Q15738	Homo sapiens	31%	97%
Q32L94	Bos taurus	26%	92%
Q3ZBE9	Bos taurus	30%	100%
Q4QJ44	Leishmania major	92%	100%
Q4R7R1	Macaca fascicularis	25%	92%
Q5A1B0	Candida albicans (strain SC5314 / ATCC MYA-2876)	26%	100%
Q5PPL3	Rattus norvegicus	29%	100%
Q8EG63	Shewanella oneidensis (strain MR-1)	24%	94%
Q8PDW5	Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)	29%	100%
Q8WUS8	Homo sapiens	25%	92%
Q9D665	Mus musculus	28%	92%
Q9FX01	Arabidopsis thaliana	31%	83%
Q9R1J0	Mus musculus	28%	100%
Q9XWF0	Caenorhabditis elegans	25%	79%
V5BEU6	Trypanosoma cruzi	72%	89%