V-type proton ATPase subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

V-type proton ATPase subunit D, putative

Species:

Leishmania braziliensis

UniProt:

A4H4F9_LEIBR

TriTrypDb:

LbrM.05.1140 , LBRM2903_050017400

Length:

357

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	12
GO:0033177	proton-transporting two-sector ATPase complex, proton-transporting domain	3	12
GO:0033179	proton-transporting V-type ATPase, V0 domain	4	12
GO:0098796	membrane protein complex	2	12
GO:0000323	lytic vacuole	6	1
GO:0005764	lysosome	7	1
GO:0005773	vacuole	5	1
GO:0005794	Golgi apparatus	5	1
GO:0016469	proton-transporting two-sector ATPase complex	3	1
GO:0016471	vacuolar proton-transporting V-type ATPase complex	5	1
GO:0020022	acidocalcisome	5	1
GO:0033176	proton-transporting V-type ATPase complex	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H4F9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4F9

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006873	intracellular monoatomic ion homeostasis	4	1
GO:0006885	regulation of pH	8	1
GO:0007034	vacuolar transport	4	1
GO:0007035	vacuolar acidification	10	1
GO:0009987	cellular process	1	1
GO:0019725	cellular homeostasis	2	1
GO:0030003	intracellular monoatomic cation homeostasis	5	1
GO:0030004	obsolete cellular monovalent inorganic cation homeostasis	6	1
GO:0030641	regulation of cellular pH	7	1
GO:0042592	homeostatic process	3	1
GO:0046907	intracellular transport	3	1
GO:0048878	chemical homeostasis	4	1
GO:0050801	monoatomic ion homeostasis	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051452	intracellular pH reduction	9	1
GO:0051453	regulation of intracellular pH	8	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0055067	obsolete monovalent inorganic cation homeostasis	7	1
GO:0055080	monoatomic cation homeostasis	6	1
GO:0055082	intracellular chemical homeostasis	3	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0098771	inorganic ion homeostasis	6	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	12
GO:0008324	monoatomic cation transmembrane transporter activity	4	12
GO:0009678	pyrophosphate hydrolysis-driven proton transmembrane transporter activity	5	12
GO:0015075	monoatomic ion transmembrane transporter activity	3	12
GO:0015078	proton transmembrane transporter activity	5	12
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	12
GO:0015399	primary active transmembrane transporter activity	4	12
GO:0019829	ATPase-coupled monoatomic cation transmembrane transporter activity	3	12
GO:0022804	active transmembrane transporter activity	3	12
GO:0022853	active monoatomic ion transmembrane transporter activity	4	12
GO:0022857	transmembrane transporter activity	2	12
GO:0022890	inorganic cation transmembrane transporter activity	4	12
GO:0042625	ATPase-coupled ion transmembrane transporter activity	3	12
GO:0042626	ATPase-coupled transmembrane transporter activity	2	12
GO:0044769	ATPase activity, coupled to transmembrane movement of ions, rotational mechanism	4	12
GO:0046961	proton-transporting ATPase activity, rotational mechanism	4	12
GO:0140657	ATP-dependent activity	1	12
GO:0003824	catalytic activity	1	2
GO:0016787	hydrolase activity	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	237	239	PF00675	0.312
CLV_NRD_NRD_1	268	270	PF00675	0.310
CLV_NRD_NRD_1	294	296	PF00675	0.380
CLV_PCSK_FUR_1	266	270	PF00082	0.294
CLV_PCSK_KEX2_1	114	116	PF00082	0.288
CLV_PCSK_KEX2_1	237	239	PF00082	0.310
CLV_PCSK_KEX2_1	268	270	PF00082	0.294
CLV_PCSK_KEX2_1	294	296	PF00082	0.294
CLV_PCSK_PC1ET2_1	114	116	PF00082	0.294
CLV_PCSK_PC7_1	264	270	PF00082	0.310
CLV_PCSK_SKI1_1	181	185	PF00082	0.310
CLV_PCSK_SKI1_1	217	221	PF00082	0.329
CLV_PCSK_SKI1_1	23	27	PF00082	0.415
CLV_PCSK_SKI1_1	230	234	PF00082	0.299
CLV_PCSK_SKI1_1	238	242	PF00082	0.294
CLV_PCSK_SKI1_1	268	272	PF00082	0.318
DOC_MAPK_gen_1	294	302	PF00069	0.380
DOC_USP7_MATH_1	113	117	PF00917	0.294
DOC_USP7_MATH_1	283	287	PF00917	0.384
DOC_WW_Pin1_4	153	158	PF00397	0.294
DOC_WW_Pin1_4	230	235	PF00397	0.384
LIG_14-3-3_CanoR_1	160	168	PF00244	0.329
LIG_14-3-3_CanoR_1	217	223	PF00244	0.297
LIG_APCC_ABBA_1	130	135	PF00400	0.415
LIG_APCC_ABBA_1	248	253	PF00400	0.294
LIG_APCC_ABBA_1	301	306	PF00400	0.310
LIG_eIF4E_1	179	185	PF01652	0.310
LIG_FHA_1	201	207	PF00498	0.294
LIG_FHA_1	219	225	PF00498	0.294
LIG_FHA_1	62	68	PF00498	0.280
LIG_FHA_2	117	123	PF00498	0.189
LIG_FHA_2	231	237	PF00498	0.368
LIG_LIR_Apic_2	85	89	PF02991	0.329
LIG_LIR_Gen_1	119	129	PF02991	0.189
LIG_LIR_Gen_1	147	157	PF02991	0.295
LIG_LIR_Gen_1	271	279	PF02991	0.318
LIG_LIR_Gen_1	30	39	PF02991	0.294
LIG_LIR_LC3C_4	298	302	PF02991	0.189
LIG_LIR_Nem_3	119	124	PF02991	0.189
LIG_LIR_Nem_3	147	152	PF02991	0.306
LIG_LIR_Nem_3	190	195	PF02991	0.296
LIG_LIR_Nem_3	271	277	PF02991	0.345
LIG_LIR_Nem_3	30	35	PF02991	0.294
LIG_LIR_Nem_3	51	56	PF02991	0.294
LIG_Pex14_2	270	274	PF04695	0.415
LIG_SH2_CRK	318	322	PF00017	0.380
LIG_SH2_PTP2	320	323	PF00017	0.380
LIG_SH2_SRC	318	321	PF00017	0.380
LIG_SH2_STAP1	189	193	PF00017	0.333
LIG_SH2_STAT3	188	191	PF00017	0.310
LIG_SH2_STAT5	179	182	PF00017	0.294
LIG_SH2_STAT5	273	276	PF00017	0.441
LIG_SH2_STAT5	320	323	PF00017	0.380
LIG_SH2_STAT5	349	352	PF00017	0.391
LIG_SH3_3	131	137	PF00018	0.415
LIG_SH3_3	203	209	PF00018	0.287
LIG_SUMO_SIM_anti_2	150	156	PF11976	0.329
LIG_SUMO_SIM_anti_2	298	304	PF11976	0.189
LIG_SUMO_SIM_par_1	150	156	PF11976	0.306
LIG_SUMO_SIM_par_1	246	253	PF11976	0.310
LIG_TYR_ITIM	316	321	PF00017	0.310
LIG_WRC_WIRS_1	219	224	PF05994	0.329
LIG_WRC_WIRS_1	53	58	PF05994	0.189
MOD_CDK_SPxxK_3	153	160	PF00069	0.294
MOD_CDK_SPxxK_3	230	237	PF00069	0.415
MOD_CK1_1	116	122	PF00069	0.328
MOD_CK1_1	288	294	PF00069	0.394
MOD_CK2_1	116	122	PF00069	0.308
MOD_CK2_1	254	260	PF00069	0.415
MOD_CK2_1	82	88	PF00069	0.441
MOD_GlcNHglycan	115	118	PF01048	0.307
MOD_GlcNHglycan	162	165	PF01048	0.329
MOD_GlcNHglycan	285	288	PF01048	0.439
MOD_GSK3_1	39	46	PF00069	0.316
MOD_GSK3_1	48	55	PF00069	0.264
MOD_N-GLC_1	39	44	PF02516	0.189
MOD_NEK2_1	1	6	PF00069	0.622
MOD_NEK2_1	218	223	PF00069	0.333
MOD_NEK2_1	277	282	PF00069	0.317
MOD_PIKK_1	43	49	PF00454	0.310
MOD_PKA_1	268	274	PF00069	0.189
MOD_PKA_2	268	274	PF00069	0.189
MOD_PKB_1	266	274	PF00069	0.189
MOD_Plk_1	173	179	PF00069	0.310
MOD_Plk_2-3	173	179	PF00069	0.310
MOD_Plk_2-3	82	88	PF00069	0.441
MOD_Plk_4	135	141	PF00069	0.380
MOD_Plk_4	243	249	PF00069	0.294
MOD_Plk_4	52	58	PF00069	0.347
MOD_ProDKin_1	153	159	PF00069	0.294
MOD_ProDKin_1	230	236	PF00069	0.384
MOD_SUMO_rev_2	39	46	PF00179	0.294
TRG_DiLeu_BaEn_1	135	140	PF01217	0.368
TRG_DiLeu_BaLyEn_6	206	211	PF01217	0.329
TRG_ENDOCYTIC_2	189	192	PF00928	0.340
TRG_ENDOCYTIC_2	273	276	PF00928	0.415
TRG_ENDOCYTIC_2	314	317	PF00928	0.294
TRG_ENDOCYTIC_2	318	321	PF00928	0.294
TRG_ENDOCYTIC_2	32	35	PF00928	0.278
TRG_ENDOCYTIC_2	53	56	PF00928	0.294
TRG_ER_diArg_1	266	269	PF00400	0.310
TRG_ER_diArg_1	294	296	PF00400	0.380
TRG_ER_diArg_1	342	345	PF00400	0.415
TRG_NES_CRM1_1	265	279	PF08389	0.386
TRG_NES_CRM1_1	39	52	PF08389	0.294
TRG_Pf-PMV_PEXEL_1	170	174	PF00026	0.320
TRG_Pf-PMV_PEXEL_1	66	70	PF00026	0.334

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IHC7	Leptomonas seymouri	89%	100%
A0A0S4INJ3	Bodo saltans	63%	100%
A0A1X0NK55	Trypanosomatidae	70%	98%
A0A3S7WP99	Leishmania donovani	94%	100%
A0A422N530	Trypanosoma rangeli	69%	100%
A4HSN6	Leishmania infantum	94%	100%
C9ZNE1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	93%
E9AKM1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O13753	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	39%	100%
P32366	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	34%	100%
P51863	Mus musculus	41%	100%
P53659	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	34%	98%
P54641	Dictyostelium discoideum	38%	100%
P61420	Bos taurus	41%	100%
P61421	Homo sapiens	41%	100%
Q25531	Manduca sexta	39%	100%
Q2KJB6	Bos taurus	39%	100%
Q4QJ88	Leishmania major	94%	100%
Q5FVL0	Rattus norvegicus	40%	100%
Q5R6I1	Pongo abelii	41%	100%
Q5R7B7	Pongo abelii	39%	100%
Q5ZHL0	Gallus gallus	39%	100%
Q6P335	Xenopus tropicalis	39%	100%
Q6PGV1	Danio rerio	40%	100%
Q80SY3	Mus musculus	40%	100%
Q8N8Y2	Homo sapiens	39%	100%
Q8RU33	Oryza sativa subsp. japonica	38%	100%
Q9LHA4	Arabidopsis thaliana	37%	100%
Q9LJI5	Arabidopsis thaliana	37%	100%
Q9VCQ3	Drosophila melanogaster	34%	100%
Q9W4P5	Drosophila melanogaster	40%	100%
V5ARU6	Trypanosoma cruzi	68%	100%