DNA-directed RNA polymerase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerase I largest subunit

Species:

Leishmania braziliensis

UniProt:

A4H4F6_LEIBR

TriTrypDb:

LbrM.05.1110 , LBRM2903_160021600

Length:

250

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 1
NetGPI	no	yes: 0, no: 1

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	2
GO:0030880	RNA polymerase complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1990234	transferase complex	3	2

Expansion

Sequence features

A4H4F6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4F6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006351	DNA-templated transcription	7	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009058	biosynthetic process	2	2
GO:0009059	macromolecule biosynthetic process	4	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0018130	heterocycle biosynthetic process	4	2
GO:0019438	aromatic compound biosynthetic process	4	2
GO:0032774	RNA biosynthetic process	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034654	nucleobase-containing compound biosynthetic process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:0097659	nucleic acid-templated transcription	6	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	2
GO:0003677	DNA binding	4	2
GO:0003824	catalytic activity	1	2
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	2
GO:0005488	binding	1	2
GO:0016740	transferase activity	2	2
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	2
GO:0016779	nucleotidyltransferase activity	4	2
GO:0034062	5'-3' RNA polymerase activity	5	2
GO:0097159	organic cyclic compound binding	2	2
GO:0097747	RNA polymerase activity	4	2
GO:0140098	catalytic activity, acting on RNA	3	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2
GO:1901363	heterocyclic compound binding	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_FUR_1	11	15	PF00082	0.850
CLV_PCSK_KEX2_1	121	123	PF00082	0.449
CLV_PCSK_KEX2_1	13	15	PF00082	0.855
CLV_PCSK_KEX2_1	170	172	PF00082	0.449
CLV_PCSK_PC1ET2_1	121	123	PF00082	0.449
CLV_PCSK_PC1ET2_1	13	15	PF00082	0.855
CLV_PCSK_PC1ET2_1	170	172	PF00082	0.449
CLV_PCSK_SKI1_1	150	154	PF00082	0.449
CLV_PCSK_SKI1_1	165	169	PF00082	0.262
CLV_PCSK_SKI1_1	227	231	PF00082	0.449
DEG_COP1_1	194	205	PF00400	0.650
DEG_COP1_1	36	45	PF00400	0.707
DEG_Nend_UBRbox_1	1	4	PF02207	0.700
DOC_CYCLIN_RxL_1	146	156	PF00134	0.650
DOC_PP1_RVXF_1	148	155	PF00149	0.650
DOC_PP2B_LxvP_1	200	203	PF13499	0.650
DOC_USP7_MATH_1	67	71	PF00917	0.674
DOC_WW_Pin1_4	129	134	PF00397	0.650
DOC_WW_Pin1_4	159	164	PF00397	0.650
DOC_WW_Pin1_4	219	224	PF00397	0.650
DOC_WW_Pin1_4	68	73	PF00397	0.704
LIG_14-3-3_CanoR_1	150	155	PF00244	0.650
LIG_14-3-3_CanoR_1	48	57	PF00244	0.645
LIG_FHA_1	45	51	PF00498	0.673
LIG_FHA_1	99	105	PF00498	0.654
LIG_FHA_2	151	157	PF00498	0.650
LIG_FHA_2	182	188	PF00498	0.650
LIG_FHA_2	189	195	PF00498	0.562
LIG_FHA_2	77	83	PF00498	0.701
LIG_LIR_Gen_1	194	205	PF02991	0.650
LIG_LIR_Gen_1	98	108	PF02991	0.658
LIG_LIR_Nem_3	153	157	PF02991	0.650
LIG_LIR_Nem_3	194	200	PF02991	0.650
LIG_LIR_Nem_3	61	66	PF02991	0.653
LIG_LIR_Nem_3	98	103	PF02991	0.654
LIG_PCNA_yPIPBox_3	90	100	PF02747	0.655
LIG_SH2_CRK	100	104	PF00017	0.661
LIG_SH2_GRB2like	100	103	PF00017	0.661
LIG_SH2_STAP1	100	104	PF00017	0.661
LIG_SH2_STAT5	100	103	PF00017	0.661
LIG_SH2_STAT5	135	138	PF00017	0.650
LIG_SH2_STAT5	214	217	PF00017	0.650
LIG_SH2_STAT5	64	67	PF00017	0.659
LIG_SH3_3	127	133	PF00018	0.650
LIG_SH3_3	139	145	PF00018	0.500
LIG_TRAF2_1	79	82	PF00917	0.711
LIG_TRFH_1	135	139	PF08558	0.650
LIG_UBA3_1	113	121	PF00899	0.687
LIG_UBA3_1	166	170	PF00899	0.650
LIG_WRC_WIRS_1	104	109	PF05994	0.664
MOD_CDC14_SPxK_1	162	165	PF00782	0.650
MOD_CDK_SPxK_1	159	165	PF00069	0.650
MOD_CK1_1	192	198	PF00069	0.650
MOD_CK2_1	150	156	PF00069	0.650
MOD_CK2_1	181	187	PF00069	0.650
MOD_CK2_1	188	194	PF00069	0.562
MOD_CK2_1	67	73	PF00069	0.696
MOD_CK2_1	76	82	PF00069	0.545
MOD_Cter_Amidation	11	14	PF01082	0.850
MOD_Cter_Amidation	119	122	PF01082	0.549
MOD_GlcNHglycan	191	194	PF01048	0.449
MOD_GlcNHglycan	30	33	PF01048	0.755
MOD_GlcNHglycan	37	40	PF01048	0.610
MOD_GlcNHglycan	6	10	PF01048	0.831
MOD_GSK3_1	188	195	PF00069	0.650
MOD_GSK3_1	217	224	PF00069	0.650
MOD_GSK3_1	33	40	PF00069	0.729
MOD_GSK3_1	44	51	PF00069	0.476
MOD_N-GLC_1	67	72	PF02516	0.687
MOD_NEK2_1	157	162	PF00069	0.650
MOD_NEK2_1	186	191	PF00069	0.650
MOD_NEK2_1	217	222	PF00069	0.650
MOD_NEK2_1	244	249	PF00069	0.650
MOD_NEK2_1	76	81	PF00069	0.703
MOD_PKA_2	28	34	PF00069	0.767
MOD_Plk_1	186	192	PF00069	0.650
MOD_Plk_4	239	245	PF00069	0.650
MOD_Plk_4	37	43	PF00069	0.718
MOD_ProDKin_1	129	135	PF00069	0.650
MOD_ProDKin_1	159	165	PF00069	0.650
MOD_ProDKin_1	219	225	PF00069	0.650
MOD_ProDKin_1	68	74	PF00069	0.706
MOD_SUMO_for_1	108	111	PF00179	0.689
TRG_ENDOCYTIC_2	100	103	PF00928	0.661
TRG_ENDOCYTIC_2	214	217	PF00928	0.650
TRG_ENDOCYTIC_2	63	66	PF00928	0.653
TRG_Pf-PMV_PEXEL_1	227	231	PF00026	0.449
TRG_Pf-PMV_PEXEL_1	48	52	PF00026	0.661

Homologs


Protein	Taxonomy	Sequence identity	Coverage
P28363	EUPOC	39%	39%
P28364	EUPOC	27%	52%