Putative ubiquitin-conjugating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania braziliensis

UniProt:

A4H4D6_LEIBR

TriTrypDb:

LbrM.05.0910 , LBRM2903_050015100

Length:

458

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0016020	membrane	2	2
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A4H4D6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4D6

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	4
GO:0016874	ligase activity	2	4
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	325	329	PF00656	0.649
CLV_NRD_NRD_1	284	286	PF00675	0.476
CLV_NRD_NRD_1	450	452	PF00675	0.374
CLV_PCSK_KEX2_1	284	286	PF00082	0.445
CLV_PCSK_KEX2_1	450	452	PF00082	0.374
CLV_PCSK_KEX2_1	9	11	PF00082	0.269
CLV_PCSK_PC1ET2_1	9	11	PF00082	0.318
CLV_PCSK_PC7_1	280	286	PF00082	0.443
CLV_PCSK_SKI1_1	334	338	PF00082	0.470
CLV_PCSK_SKI1_1	416	420	PF00082	0.500
DEG_APCC_DBOX_1	449	457	PF00400	0.374
DEG_SCF_FBW7_1	212	219	PF00400	0.660
DEG_SPOP_SBC_1	216	220	PF00917	0.635
DOC_MAPK_MEF2A_6	135	142	PF00069	0.491
DOC_MAPK_RevD_3	436	451	PF00069	0.367
DOC_PP1_RVXF_1	449	456	PF00149	0.231
DOC_PP2B_LxvP_1	249	252	PF13499	0.737
DOC_PP2B_LxvP_1	73	76	PF13499	0.518
DOC_SPAK_OSR1_1	86	90	PF12202	0.469
DOC_USP7_MATH_1	216	220	PF00917	0.696
DOC_USP7_MATH_1	301	305	PF00917	0.687
DOC_WW_Pin1_4	174	179	PF00397	0.646
DOC_WW_Pin1_4	212	217	PF00397	0.699
DOC_WW_Pin1_4	241	246	PF00397	0.736
DOC_WW_Pin1_4	250	255	PF00397	0.664
DOC_WW_Pin1_4	259	264	PF00397	0.618
DOC_WW_Pin1_4	279	284	PF00397	0.683
DOC_WW_Pin1_4	303	308	PF00397	0.758
DOC_WW_Pin1_4	314	319	PF00397	0.687
DOC_WW_Pin1_4	348	353	PF00397	0.718
DOC_WW_Pin1_4	362	367	PF00397	0.677
DOC_WW_Pin1_4	394	399	PF00397	0.709
LIG_Actin_RPEL_3	428	447	PF02755	0.417
LIG_Actin_WH2_2	434	452	PF00022	0.503
LIG_APCC_ABBA_1	170	175	PF00400	0.605
LIG_BIR_II_1	1	5	PF00653	0.555
LIG_BIR_III_4	342	346	PF00653	0.627
LIG_BRCT_BRCA1_1	83	87	PF00533	0.469
LIG_deltaCOP1_diTrp_1	144	153	PF00928	0.569
LIG_deltaCOP1_diTrp_1	39	42	PF00928	0.469
LIG_EVH1_2	264	268	PF00568	0.643
LIG_FHA_1	106	112	PF00498	0.518
LIG_FHA_1	160	166	PF00498	0.552
LIG_FHA_1	304	310	PF00498	0.735
LIG_FHA_1	394	400	PF00498	0.719
LIG_FHA_2	159	165	PF00498	0.507
LIG_FHA_2	377	383	PF00498	0.660
LIG_GBD_Chelix_1	438	446	PF00786	0.374
LIG_HCF-1_HBM_1	374	377	PF13415	0.644
LIG_LIR_Apic_2	27	32	PF02991	0.469
LIG_LIR_LC3C_4	440	445	PF02991	0.231
LIG_LIR_Nem_3	12	18	PF02991	0.518
LIG_LIR_Nem_3	266	271	PF02991	0.649
LIG_LYPXL_S_1	172	176	PF13949	0.304
LIG_LYPXL_yS_3	173	176	PF13949	0.496
LIG_MYND_1	185	189	PF01753	0.653
LIG_NRBOX	452	458	PF00104	0.451
LIG_Pex14_1	105	109	PF04695	0.469
LIG_Pex14_2	38	42	PF04695	0.469
LIG_REV1ctd_RIR_1	78	87	PF16727	0.518
LIG_SH2_CRK	29	33	PF00017	0.433
LIG_SH2_SRC	109	112	PF00017	0.537
LIG_SH2_STAP1	109	113	PF00017	0.469
LIG_SH2_STAP1	3	7	PF00017	0.573
LIG_SH2_STAP1	377	381	PF00017	0.679
LIG_SH2_STAT5	346	349	PF00017	0.616
LIG_SH3_1	69	75	PF00018	0.469
LIG_SH3_3	182	188	PF00018	0.686
LIG_SH3_3	249	255	PF00018	0.742
LIG_SH3_3	353	359	PF00018	0.760
LIG_SH3_3	363	369	PF00018	0.733
LIG_SH3_3	370	376	PF00018	0.672
LIG_SH3_3	405	411	PF00018	0.689
LIG_SH3_3	423	429	PF00018	0.534
LIG_SH3_3	69	75	PF00018	0.469
LIG_SUMO_SIM_anti_2	440	446	PF11976	0.231
LIG_SUMO_SIM_par_1	136	141	PF11976	0.547
LIG_TRFH_1	71	75	PF08558	0.518
MOD_CDC14_SPxK_1	177	180	PF00782	0.633
MOD_CDC14_SPxK_1	282	285	PF00782	0.660
MOD_CDC14_SPxK_1	351	354	PF00782	0.701
MOD_CDK_SPK_2	279	284	PF00069	0.686
MOD_CDK_SPxK_1	174	180	PF00069	0.623
MOD_CDK_SPxK_1	279	285	PF00069	0.665
MOD_CDK_SPxK_1	348	354	PF00069	0.705
MOD_CK1_1	2	8	PF00069	0.627
MOD_CK1_1	214	220	PF00069	0.711
MOD_CK1_1	225	231	PF00069	0.631
MOD_CK1_1	250	256	PF00069	0.643
MOD_CK1_1	279	285	PF00069	0.654
MOD_CK1_1	330	336	PF00069	0.801
MOD_CK1_1	397	403	PF00069	0.655
MOD_CK1_1	95	101	PF00069	0.483
MOD_CK2_1	376	382	PF00069	0.623
MOD_GlcNHglycan	196	199	PF01048	0.573
MOD_GlcNHglycan	249	252	PF01048	0.444
MOD_GlcNHglycan	276	279	PF01048	0.450
MOD_GlcNHglycan	328	332	PF01048	0.637
MOD_GlcNHglycan	83	86	PF01048	0.287
MOD_GSK3_1	149	156	PF00069	0.548
MOD_GSK3_1	190	197	PF00069	0.678
MOD_GSK3_1	211	218	PF00069	0.823
MOD_GSK3_1	225	232	PF00069	0.660
MOD_GSK3_1	310	317	PF00069	0.686
MOD_GSK3_1	376	383	PF00069	0.675
MOD_GSK3_1	393	400	PF00069	0.619
MOD_GSK3_1	92	99	PF00069	0.478
MOD_LATS_1	90	96	PF00433	0.417
MOD_NEK2_1	113	118	PF00069	0.518
MOD_NEK2_1	153	158	PF00069	0.584
MOD_NEK2_1	247	252	PF00069	0.646
MOD_NEK2_1	271	276	PF00069	0.651
MOD_NEK2_1	302	307	PF00069	0.710
MOD_NEK2_1	361	366	PF00069	0.634
MOD_NEK2_1	424	429	PF00069	0.739
MOD_NEK2_1	65	70	PF00069	0.572
MOD_NEK2_1	79	84	PF00069	0.473
MOD_NEK2_2	40	45	PF00069	0.518
MOD_PKA_1	9	15	PF00069	0.518
MOD_PKA_2	9	15	PF00069	0.553
MOD_Plk_1	310	316	PF00069	0.659
MOD_Plk_1	424	430	PF00069	0.678
MOD_Plk_1	50	56	PF00069	0.557
MOD_Plk_4	218	224	PF00069	0.675
MOD_Plk_4	380	386	PF00069	0.699
MOD_Plk_4	437	443	PF00069	0.309
MOD_ProDKin_1	174	180	PF00069	0.646
MOD_ProDKin_1	212	218	PF00069	0.698
MOD_ProDKin_1	241	247	PF00069	0.737
MOD_ProDKin_1	250	256	PF00069	0.663
MOD_ProDKin_1	259	265	PF00069	0.617
MOD_ProDKin_1	279	285	PF00069	0.694
MOD_ProDKin_1	303	309	PF00069	0.760
MOD_ProDKin_1	314	320	PF00069	0.687
MOD_ProDKin_1	348	354	PF00069	0.719
MOD_ProDKin_1	362	368	PF00069	0.676
MOD_ProDKin_1	394	400	PF00069	0.713
MOD_SUMO_for_1	336	339	PF00179	0.677
MOD_SUMO_rev_2	164	172	PF00179	0.493
TRG_ENDOCYTIC_2	15	18	PF00928	0.518
TRG_ENDOCYTIC_2	173	176	PF00928	0.496
TRG_ER_diArg_1	283	285	PF00400	0.636
TRG_ER_diArg_1	449	451	PF00400	0.374

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3S5H5K9	Leishmania donovani	59%	99%
A4HSL5	Leishmania infantum	59%	98%
Q4QJA9	Leishmania major	60%	95%
V5B702	Trypanosoma cruzi	39%	100%