tRNA 4-demethylwyosine synthase (AdoMet-dependent)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Flavodoxin/Radical SAM superfamily/Wyosine base formation, putative

Species:

Leishmania braziliensis

UniProt:

A4H4B8_LEIBR

TriTrypDb:

LbrM.05.0730 , LBRM2903_050013100

Length:

844

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	8
GO:0110165	cellular anatomical entity	1	8
GO:0016020	membrane	2	1

Expansion

Sequence features

A4H4B8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H4B8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	8
GO:0006396	RNA processing	6	8
GO:0006399	tRNA metabolic process	7	8
GO:0006725	cellular aromatic compound metabolic process	3	8
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008033	tRNA processing	8	8
GO:0008152	metabolic process	1	8
GO:0009987	cellular process	1	8
GO:0016070	RNA metabolic process	5	8
GO:0034470	ncRNA processing	7	8
GO:0034641	cellular nitrogen compound metabolic process	3	8
GO:0034660	ncRNA metabolic process	6	8
GO:0043170	macromolecule metabolic process	3	8
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0046483	heterocycle metabolic process	3	8
GO:0071704	organic substance metabolic process	2	8
GO:0090304	nucleic acid metabolic process	4	8
GO:1901360	organic cyclic compound metabolic process	3	8
GO:0006400	tRNA modification	6	1
GO:0009058	biosynthetic process	2	1
GO:0009451	RNA modification	5	1
GO:0031590	wybutosine metabolic process	4	1
GO:0031591	wybutosine biosynthetic process	5	1
GO:0043412	macromolecule modification	4	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901137	carbohydrate derivative biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1
GO:1901657	glycosyl compound metabolic process	4	1
GO:1901659	glycosyl compound biosynthetic process	5	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	8
GO:0003824	catalytic activity	1	8
GO:0005488	binding	1	8
GO:0010181	FMN binding	4	8
GO:0016829	lyase activity	2	8
GO:0032553	ribonucleotide binding	3	8
GO:0036094	small molecule binding	2	8
GO:0043167	ion binding	2	8
GO:0043168	anion binding	3	8
GO:0043169	cation binding	3	8
GO:0046872	metal ion binding	4	8
GO:0051536	iron-sulfur cluster binding	3	8
GO:0051539	4 iron, 4 sulfur cluster binding	4	8
GO:0051540	metal cluster binding	2	8
GO:0097159	organic cyclic compound binding	2	8
GO:0097367	carbohydrate derivative binding	2	8
GO:1901265	nucleoside phosphate binding	3	8
GO:1901363	heterocyclic compound binding	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	358	362	PF00656	0.717
CLV_C14_Caspase3-7	695	699	PF00656	0.311
CLV_NRD_NRD_1	218	220	PF00675	0.501
CLV_NRD_NRD_1	831	833	PF00675	0.638
CLV_PCSK_KEX2_1	220	222	PF00082	0.421
CLV_PCSK_KEX2_1	382	384	PF00082	0.565
CLV_PCSK_KEX2_1	620	622	PF00082	0.356
CLV_PCSK_PC1ET2_1	220	222	PF00082	0.421
CLV_PCSK_PC1ET2_1	382	384	PF00082	0.565
CLV_PCSK_PC1ET2_1	620	622	PF00082	0.356
CLV_PCSK_SKI1_1	463	467	PF00082	0.429
CLV_PCSK_SKI1_1	497	501	PF00082	0.317
CLV_PCSK_SKI1_1	548	552	PF00082	0.319
CLV_PCSK_SKI1_1	599	603	PF00082	0.317
CLV_PCSK_SKI1_1	624	628	PF00082	0.317
CLV_PCSK_SKI1_1	647	651	PF00082	0.317
CLV_PCSK_SKI1_1	681	685	PF00082	0.376
CLV_PCSK_SKI1_1	774	778	PF00082	0.431
DEG_APCC_DBOX_1	48	56	PF00400	0.593
DEG_MDM2_SWIB_1	627	635	PF02201	0.517
DEG_MDM2_SWIB_1	777	785	PF02201	0.469
DEG_Nend_Nbox_1	1	3	PF02207	0.605
DEG_SPOP_SBC_1	123	127	PF00917	0.470
DOC_MAPK_DCC_7	514	524	PF00069	0.556
DOC_MAPK_gen_1	599	609	PF00069	0.496
DOC_MAPK_gen_1	643	654	PF00069	0.519
DOC_MAPK_MEF2A_6	516	524	PF00069	0.556
DOC_MAPK_MEF2A_6	599	607	PF00069	0.522
DOC_PP2B_LxvP_1	251	254	PF13499	0.430
DOC_PP2B_LxvP_1	55	58	PF13499	0.475
DOC_PP2B_LxvP_1	611	614	PF13499	0.426
DOC_PP4_FxxP_1	256	259	PF00568	0.477
DOC_SPAK_OSR1_1	221	225	PF12202	0.421
DOC_USP7_MATH_1	151	155	PF00917	0.508
DOC_USP7_MATH_1	308	312	PF00917	0.761
DOC_USP7_MATH_1	32	36	PF00917	0.566
DOC_USP7_MATH_1	353	357	PF00917	0.685
DOC_USP7_MATH_1	75	79	PF00917	0.646
DOC_USP7_MATH_1	98	102	PF00917	0.481
DOC_USP7_UBL2_3	639	643	PF12436	0.496
DOC_WW_Pin1_4	486	491	PF00397	0.406
DOC_WW_Pin1_4	542	547	PF00397	0.517
DOC_WW_Pin1_4	612	617	PF00397	0.504
DOC_WW_Pin1_4	76	81	PF00397	0.694
DOC_WW_Pin1_4	807	812	PF00397	0.443
LIG_14-3-3_CanoR_1	214	218	PF00244	0.430
LIG_14-3-3_CanoR_1	273	283	PF00244	0.480
LIG_14-3-3_CanoR_1	285	289	PF00244	0.381
LIG_14-3-3_CanoR_1	460	466	PF00244	0.493
LIG_14-3-3_CanoR_1	548	555	PF00244	0.533
LIG_14-3-3_CanoR_1	633	641	PF00244	0.551
LIG_14-3-3_CanoR_1	651	655	PF00244	0.422
LIG_Actin_WH2_2	267	283	PF00022	0.493
LIG_Actin_WH2_2	604	622	PF00022	0.556
LIG_BRCT_BRCA1_1	195	199	PF00533	0.376
LIG_BRCT_BRCA1_1	509	513	PF00533	0.556
LIG_Clathr_ClatBox_1	769	773	PF01394	0.444
LIG_deltaCOP1_diTrp_1	778	784	PF00928	0.470
LIG_FHA_1	114	120	PF00498	0.609
LIG_FHA_1	186	192	PF00498	0.450
LIG_FHA_1	266	272	PF00498	0.509
LIG_FHA_1	275	281	PF00498	0.228
LIG_FHA_1	291	297	PF00498	0.350
LIG_FHA_1	311	317	PF00498	0.473
LIG_FHA_1	369	375	PF00498	0.510
LIG_FHA_1	487	493	PF00498	0.350
LIG_FHA_1	504	510	PF00498	0.371
LIG_FHA_1	658	664	PF00498	0.517
LIG_FHA_1	826	832	PF00498	0.644
LIG_FHA_2	124	130	PF00498	0.496
LIG_FHA_2	168	174	PF00498	0.430
LIG_FHA_2	356	362	PF00498	0.598
LIG_FHA_2	417	423	PF00498	0.501
LIG_FHA_2	613	619	PF00498	0.517
LIG_FHA_2	693	699	PF00498	0.311
LIG_FHA_2	781	787	PF00498	0.451
LIG_FHA_2	800	806	PF00498	0.278
LIG_GBD_Chelix_1	18	26	PF00786	0.557
LIG_LIR_Gen_1	175	184	PF02991	0.249
LIG_LIR_Gen_1	196	207	PF02991	0.376
LIG_LIR_Gen_1	234	244	PF02991	0.374
LIG_LIR_Gen_1	676	685	PF02991	0.405
LIG_LIR_Gen_1	786	794	PF02991	0.438
LIG_LIR_Gen_1	799	809	PF02991	0.439
LIG_LIR_Nem_3	175	180	PF02991	0.315
LIG_LIR_Nem_3	234	240	PF02991	0.420
LIG_LIR_Nem_3	270	275	PF02991	0.453
LIG_LIR_Nem_3	432	438	PF02991	0.461
LIG_LIR_Nem_3	476	480	PF02991	0.415
LIG_LIR_Nem_3	676	680	PF02991	0.405
LIG_LIR_Nem_3	783	787	PF02991	0.404
LIG_LIR_Nem_3	788	794	PF02991	0.437
LIG_LIR_Nem_3	799	804	PF02991	0.459
LIG_LIR_Nem_3	805	809	PF02991	0.440
LIG_LIR_Nem_3	810	816	PF02991	0.394
LIG_LYPXL_S_1	434	438	PF13949	0.493
LIG_LYPXL_S_1	568	572	PF13949	0.317
LIG_LYPXL_yS_3	435	438	PF13949	0.502
LIG_LYPXL_yS_3	569	572	PF13949	0.517
LIG_MAD2	829	837	PF02301	0.660
LIG_NRBOX	5	11	PF00104	0.367
LIG_Pex14_2	627	631	PF04695	0.517
LIG_Pex14_2	777	781	PF04695	0.487
LIG_PTB_Apo_2	588	595	PF02174	0.517
LIG_SH2_CRK	177	181	PF00017	0.390
LIG_SH2_CRK	447	451	PF00017	0.442
LIG_SH2_CRK	532	536	PF00017	0.556
LIG_SH2_CRK	724	728	PF00017	0.584
LIG_SH2_CRK	806	810	PF00017	0.433
LIG_SH2_GRB2like	187	190	PF00017	0.249
LIG_SH2_NCK_1	806	810	PF00017	0.399
LIG_SH2_STAP1	177	181	PF00017	0.430
LIG_SH2_STAP1	262	266	PF00017	0.604
LIG_SH2_STAP1	666	670	PF00017	0.517
LIG_SH2_STAP1	787	791	PF00017	0.477
LIG_SH2_STAT3	97	100	PF00017	0.448
LIG_SH2_STAT5	187	190	PF00017	0.376
LIG_SH2_STAT5	262	265	PF00017	0.546
LIG_SH2_STAT5	608	611	PF00017	0.517
LIG_SH2_STAT5	685	688	PF00017	0.376
LIG_SH3_2	72	77	PF14604	0.567
LIG_SH3_3	10	16	PF00018	0.377
LIG_SH3_3	188	194	PF00018	0.501
LIG_SH3_3	254	260	PF00018	0.479
LIG_SH3_3	56	62	PF00018	0.549
LIG_SH3_3	560	566	PF00018	0.516
LIG_SH3_3	69	75	PF00018	0.545
LIG_SH3_3	79	85	PF00018	0.793
LIG_SH3_4	602	609	PF00018	0.496
LIG_SUMO_SIM_anti_2	740	748	PF11976	0.632
LIG_SUMO_SIM_par_1	768	773	PF11976	0.442
LIG_TRAF2_1	615	618	PF00917	0.556
LIG_TRAF2_1	748	751	PF00917	0.646
LIG_TRAF2_2	595	600	PF00917	0.490
LIG_TYR_ITIM	21	26	PF00017	0.566
LIG_TYR_ITIM	433	438	PF00017	0.487
LIG_TYR_ITIM	567	572	PF00017	0.517
LIG_TYR_ITIM	804	809	PF00017	0.497
LIG_UBA3_1	769	774	PF00899	0.451
LIG_WRC_WIRS_1	584	589	PF05994	0.538
LIG_WRC_WIRS_1	706	711	PF05994	0.249
LIG_WW_1	259	262	PF00397	0.477
LIG_WW_3	192	196	PF00397	0.430
MOD_CDK_SPxK_1	542	548	PF00069	0.517
MOD_CK1_1	101	107	PF00069	0.459
MOD_CK1_1	113	119	PF00069	0.473
MOD_CK1_1	146	152	PF00069	0.581
MOD_CK1_1	185	191	PF00069	0.376
MOD_CK1_1	284	290	PF00069	0.520
MOD_CK1_1	689	695	PF00069	0.376
MOD_CK1_1	796	802	PF00069	0.528
MOD_CK2_1	122	128	PF00069	0.469
MOD_CK2_1	167	173	PF00069	0.430
MOD_CK2_1	612	618	PF00069	0.517
MOD_CMANNOS	781	784	PF00535	0.392
MOD_GlcNHglycan	143	149	PF01048	0.512
MOD_GlcNHglycan	153	156	PF01048	0.504
MOD_GlcNHglycan	310	313	PF01048	0.810
MOD_GlcNHglycan	355	358	PF01048	0.714
MOD_GlcNHglycan	453	456	PF01048	0.404
MOD_GlcNHglycan	795	798	PF01048	0.481
MOD_GSK3_1	118	125	PF00069	0.452
MOD_GSK3_1	261	268	PF00069	0.471
MOD_GSK3_1	297	304	PF00069	0.587
MOD_GSK3_1	310	317	PF00069	0.775
MOD_GSK3_1	331	338	PF00069	0.593
MOD_GSK3_1	503	510	PF00069	0.544
MOD_GSK3_1	583	590	PF00069	0.517
MOD_GSK3_1	681	688	PF00069	0.376
MOD_GSK3_1	712	719	PF00069	0.249
MOD_GSK3_1	76	83	PF00069	0.574
MOD_GSK3_1	796	803	PF00069	0.520
MOD_N-GLC_1	480	485	PF02516	0.472
MOD_N-GLC_2	164	166	PF02516	0.430
MOD_N-GLC_2	496	498	PF02516	0.317
MOD_NEK2_1	172	177	PF00069	0.362
MOD_NEK2_1	182	187	PF00069	0.392
MOD_NEK2_1	26	31	PF00069	0.379
MOD_NEK2_1	302	307	PF00069	0.736
MOD_NEK2_1	449	454	PF00069	0.424
MOD_NEK2_1	587	592	PF00069	0.517
MOD_NEK2_1	650	655	PF00069	0.517
MOD_NEK2_1	793	798	PF00069	0.465
MOD_NEK2_1	9	14	PF00069	0.628
MOD_NEK2_2	292	297	PF00069	0.566
MOD_NEK2_2	473	478	PF00069	0.411
MOD_OFUCOSY	332	339	PF10250	0.536
MOD_OFUCOSY	354	359	PF10250	0.557
MOD_OFUCOSY	760	766	PF10250	0.416
MOD_PIKK_1	172	178	PF00454	0.380
MOD_PIKK_1	193	199	PF00454	0.430
MOD_PIKK_1	265	271	PF00454	0.609
MOD_PIKK_1	342	348	PF00454	0.764
MOD_PIKK_1	42	48	PF00454	0.535
MOD_PIKK_1	480	486	PF00454	0.432
MOD_PIKK_1	96	102	PF00454	0.463
MOD_PK_1	370	376	PF00069	0.510
MOD_PKA_1	297	303	PF00069	0.627
MOD_PKA_2	158	164	PF00069	0.311
MOD_PKA_2	213	219	PF00069	0.430
MOD_PKA_2	284	290	PF00069	0.520
MOD_PKA_2	32	38	PF00069	0.525
MOD_PKA_2	459	465	PF00069	0.445
MOD_PKA_2	650	656	PF00069	0.517
MOD_PKA_2	835	841	PF00069	0.639
MOD_Plk_1	172	178	PF00069	0.249
MOD_Plk_1	799	805	PF00069	0.511
MOD_Plk_1	825	831	PF00069	0.563
MOD_Plk_2-3	800	806	PF00069	0.495
MOD_Plk_4	167	173	PF00069	0.430
MOD_Plk_4	267	273	PF00069	0.437
MOD_Plk_4	461	467	PF00069	0.457
MOD_Plk_4	473	479	PF00069	0.368
MOD_Plk_4	558	564	PF00069	0.517
MOD_Plk_4	583	589	PF00069	0.538
MOD_Plk_4	650	656	PF00069	0.517
MOD_Plk_4	780	786	PF00069	0.403
MOD_Plk_4	835	841	PF00069	0.539
MOD_ProDKin_1	486	492	PF00069	0.376
MOD_ProDKin_1	542	548	PF00069	0.517
MOD_ProDKin_1	612	618	PF00069	0.504
MOD_ProDKin_1	76	82	PF00069	0.693
MOD_ProDKin_1	807	813	PF00069	0.443
MOD_SUMO_rev_2	596	604	PF00179	0.472
MOD_SUMO_rev_2	612	622	PF00179	0.580
MOD_SUMO_rev_2	676	683	PF00179	0.376
TRG_DiLeu_BaEn_1	429	434	PF01217	0.495
TRG_ENDOCYTIC_2	177	180	PF00928	0.390
TRG_ENDOCYTIC_2	23	26	PF00928	0.635
TRG_ENDOCYTIC_2	435	438	PF00928	0.502
TRG_ENDOCYTIC_2	447	450	PF00928	0.442
TRG_ENDOCYTIC_2	532	535	PF00928	0.556
TRG_ENDOCYTIC_2	569	572	PF00928	0.517
TRG_ENDOCYTIC_2	608	611	PF00928	0.517
TRG_ENDOCYTIC_2	666	669	PF00928	0.517
TRG_ENDOCYTIC_2	724	727	PF00928	0.397
TRG_ENDOCYTIC_2	787	790	PF00928	0.429
TRG_ENDOCYTIC_2	791	794	PF00928	0.436
TRG_ENDOCYTIC_2	806	809	PF00928	0.439
TRG_ER_diArg_1	219	222	PF00400	0.462
TRG_ER_diArg_1	466	469	PF00400	0.422
TRG_ER_diArg_1	752	755	PF00400	0.592
TRG_NES_CRM1_1	600	612	PF08389	0.490
TRG_Pf-PMV_PEXEL_1	242	246	PF00026	0.430
TRG_Pf-PMV_PEXEL_1	624	628	PF00026	0.317

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I710	Leptomonas seymouri	66%	96%
A0A3S5H5J8	Leishmania donovani	81%	100%
A4HSJ7	Leishmania infantum	81%	100%
C9ZU99	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9AKI1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	79%	100%
Q4QJC7	Leishmania major	80%	96%