Probable eukaryotic initiation factor 4A

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent RNA helicase, putative

Species:

Leishmania braziliensis

UniProt:

A4H481_LEIBR

TriTrypDb:

LbrM.05.0360 , LBRM2903_050008600

Length:

630

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005730	nucleolus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H481

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H481

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006364	rRNA processing	8	1
GO:0006396	RNA processing	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016072	rRNA metabolic process	7	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	13
GO:0003676	nucleic acid binding	3	13
GO:0003724	RNA helicase activity	3	13
GO:0003743	translation initiation factor activity	4	13
GO:0003824	catalytic activity	1	13
GO:0004386	helicase activity	2	13
GO:0005488	binding	1	13
GO:0005524	ATP binding	5	13
GO:0008135	translation factor activity, RNA binding	3	13
GO:0008186	ATP-dependent activity, acting on RNA	2	13
GO:0016787	hydrolase activity	2	13
GO:0017076	purine nucleotide binding	4	13
GO:0030554	adenyl nucleotide binding	5	13
GO:0032553	ribonucleotide binding	3	13
GO:0032555	purine ribonucleotide binding	4	13
GO:0032559	adenyl ribonucleotide binding	5	13
GO:0035639	purine ribonucleoside triphosphate binding	4	13
GO:0036094	small molecule binding	2	13
GO:0043167	ion binding	2	13
GO:0043168	anion binding	3	13
GO:0045182	translation regulator activity	1	13
GO:0090079	translation regulator activity, nucleic acid binding	2	13
GO:0097159	organic cyclic compound binding	2	13
GO:0097367	carbohydrate derivative binding	2	13
GO:0140098	catalytic activity, acting on RNA	3	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13
GO:0140657	ATP-dependent activity	1	13
GO:1901265	nucleoside phosphate binding	3	13
GO:1901363	heterocyclic compound binding	2	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	112	116	PF00656	0.476
CLV_NRD_NRD_1	182	184	PF00675	0.187
CLV_NRD_NRD_1	205	207	PF00675	0.689
CLV_NRD_NRD_1	29	31	PF00675	0.167
CLV_NRD_NRD_1	505	507	PF00675	0.535
CLV_NRD_NRD_1	533	535	PF00675	0.595
CLV_NRD_NRD_1	600	602	PF00675	0.606
CLV_NRD_NRD_1	622	624	PF00675	0.716
CLV_PCSK_FUR_1	531	535	PF00082	0.718
CLV_PCSK_FUR_1	558	562	PF00082	0.606
CLV_PCSK_KEX2_1	182	184	PF00082	0.182
CLV_PCSK_KEX2_1	205	207	PF00082	0.689
CLV_PCSK_KEX2_1	448	450	PF00082	0.257
CLV_PCSK_KEX2_1	533	535	PF00082	0.574
CLV_PCSK_KEX2_1	560	562	PF00082	0.745
CLV_PCSK_KEX2_1	600	602	PF00082	0.579
CLV_PCSK_KEX2_1	624	626	PF00082	0.760
CLV_PCSK_PC1ET2_1	448	450	PF00082	0.294
CLV_PCSK_PC1ET2_1	560	562	PF00082	0.693
CLV_PCSK_PC1ET2_1	624	626	PF00082	0.760
CLV_PCSK_PC7_1	556	562	PF00082	0.493
CLV_PCSK_SKI1_1	100	104	PF00082	0.260
CLV_PCSK_SKI1_1	132	136	PF00082	0.236
CLV_PCSK_SKI1_1	170	174	PF00082	0.267
CLV_PCSK_SKI1_1	182	186	PF00082	0.199
CLV_PCSK_SKI1_1	624	628	PF00082	0.667
CLV_Separin_Metazoa	349	353	PF03568	0.428
DEG_APCC_DBOX_1	169	177	PF00400	0.350
DEG_APCC_DBOX_1	346	354	PF00400	0.431
DEG_SCF_FBW7_2	74	81	PF00400	0.417
DEG_SPOP_SBC_1	297	301	PF00917	0.606
DOC_CDC14_PxL_1	319	327	PF14671	0.397
DOC_CKS1_1	22	27	PF01111	0.493
DOC_CKS1_1	75	80	PF01111	0.417
DOC_CYCLIN_RxL_1	129	137	PF00134	0.448
DOC_CYCLIN_RxL_1	144	158	PF00134	0.407
DOC_CYCLIN_RxL_1	179	190	PF00134	0.423
DOC_CYCLIN_yCln2_LP_2	175	178	PF00134	0.483
DOC_MAPK_DCC_7	397	406	PF00069	0.428
DOC_MAPK_gen_1	179	187	PF00069	0.450
DOC_MAPK_gen_1	428	438	PF00069	0.495
DOC_MAPK_HePTP_8	394	406	PF00069	0.428
DOC_MAPK_MEF2A_6	347	355	PF00069	0.417
DOC_MAPK_MEF2A_6	397	406	PF00069	0.428
DOC_MAPK_MEF2A_6	486	493	PF00069	0.308
DOC_PP1_RVXF_1	181	188	PF00149	0.391
DOC_PP2B_LxvP_1	175	178	PF13499	0.461
DOC_PP2B_LxvP_1	198	201	PF13499	0.587
DOC_PP2B_LxvP_1	314	317	PF13499	0.333
DOC_PP2B_LxvP_1	399	402	PF13499	0.417
DOC_PP4_FxxP_1	226	229	PF00568	0.480
DOC_PP4_FxxP_1	303	306	PF00568	0.551
DOC_PP4_FxxP_1	56	59	PF00568	0.433
DOC_PP4_FxxP_1	93	96	PF00568	0.483
DOC_USP7_MATH_1	140	144	PF00917	0.350
DOC_USP7_MATH_1	208	212	PF00917	0.606
DOC_USP7_MATH_1	222	226	PF00917	0.507
DOC_USP7_MATH_1	233	237	PF00917	0.455
DOC_USP7_MATH_1	275	279	PF00917	0.663
DOC_USP7_MATH_1	297	301	PF00917	0.643
DOC_USP7_MATH_1	361	365	PF00917	0.417
DOC_USP7_MATH_1	402	406	PF00917	0.558
DOC_USP7_MATH_1	466	470	PF00917	0.438
DOC_USP7_MATH_1	47	51	PF00917	0.428
DOC_USP7_MATH_1	520	524	PF00917	0.506
DOC_USP7_MATH_1	538	542	PF00917	0.724
DOC_USP7_MATH_1	596	600	PF00917	0.614
DOC_USP7_UBL2_3	482	486	PF12436	0.280
DOC_USP7_UBL2_3	624	628	PF12436	0.627
DOC_WW_Pin1_4	124	129	PF00397	0.416
DOC_WW_Pin1_4	21	26	PF00397	0.365
DOC_WW_Pin1_4	218	223	PF00397	0.584
DOC_WW_Pin1_4	224	229	PF00397	0.620
DOC_WW_Pin1_4	460	465	PF00397	0.503
DOC_WW_Pin1_4	571	576	PF00397	0.487
DOC_WW_Pin1_4	609	614	PF00397	0.735
DOC_WW_Pin1_4	617	622	PF00397	0.769
DOC_WW_Pin1_4	74	79	PF00397	0.417
LIG_14-3-3_CanoR_1	159	166	PF00244	0.451
LIG_14-3-3_CanoR_1	194	199	PF00244	0.536
LIG_14-3-3_CanoR_1	352	356	PF00244	0.422
LIG_14-3-3_CanoR_1	457	461	PF00244	0.415
LIG_14-3-3_CanoR_1	519	527	PF00244	0.660
LIG_14-3-3_CanoR_1	558	566	PF00244	0.661
LIG_APCC_ABBA_1	406	411	PF00400	0.428
LIG_BIR_II_1	1	5	PF00653	0.339
LIG_BIR_III_4	282	286	PF00653	0.465
LIG_BIR_III_4	67	71	PF00653	0.462
LIG_BRCT_BRCA1_1	142	146	PF00533	0.350
LIG_BRCT_BRCA1_1	299	303	PF00533	0.427
LIG_EH1_1	166	174	PF00400	0.350
LIG_eIF4E_1	69	75	PF01652	0.417
LIG_FHA_1	186	192	PF00498	0.422
LIG_FHA_1	219	225	PF00498	0.472
LIG_FHA_1	256	262	PF00498	0.425
LIG_FHA_1	352	358	PF00498	0.442
LIG_FHA_2	110	116	PF00498	0.476
LIG_FHA_2	159	165	PF00498	0.414
LIG_FHA_2	194	200	PF00498	0.483
LIG_FHA_2	438	444	PF00498	0.326
LIG_FHA_2	544	550	PF00498	0.616
LIG_IRF3_LxIS_1	381	386	PF10401	0.472
LIG_LIR_Apic_2	223	229	PF02991	0.478
LIG_LIR_Apic_2	300	306	PF02991	0.600
LIG_LIR_Apic_2	459	464	PF02991	0.436
LIG_LIR_Apic_2	53	59	PF02991	0.432
LIG_LIR_Gen_1	143	154	PF02991	0.376
LIG_LIR_Gen_1	18	27	PF02991	0.413
LIG_LIR_Gen_1	243	253	PF02991	0.562
LIG_LIR_Gen_1	3	11	PF02991	0.361
LIG_LIR_Gen_1	310	320	PF02991	0.456
LIG_LIR_Gen_1	81	92	PF02991	0.417
LIG_LIR_Nem_3	143	149	PF02991	0.500
LIG_LIR_Nem_3	18	22	PF02991	0.410
LIG_LIR_Nem_3	243	248	PF02991	0.611
LIG_LIR_Nem_3	3	7	PF02991	0.340
LIG_LIR_Nem_3	310	316	PF02991	0.443
LIG_LIR_Nem_3	67	72	PF02991	0.421
LIG_LIR_Nem_3	81	87	PF02991	0.417
LIG_LIR_Nem_3	88	93	PF02991	0.417
LIG_LYPXL_SIV_4	323	331	PF13949	0.462
LIG_MYND_1	229	233	PF01753	0.483
LIG_NRBOX	130	136	PF00104	0.484
LIG_NRBOX	364	370	PF00104	0.516
LIG_RPA_C_Plants	496	507	PF08784	0.512
LIG_SH2_CRK	410	414	PF00017	0.417
LIG_SH2_GRB2like	341	344	PF00017	0.516
LIG_SH2_SRC	341	344	PF00017	0.516
LIG_SH2_SRC	84	87	PF00017	0.442
LIG_SH2_STAP1	442	446	PF00017	0.375
LIG_SH2_STAT5	151	154	PF00017	0.517
LIG_SH2_STAT5	313	316	PF00017	0.317
LIG_SH2_STAT5	324	327	PF00017	0.418
LIG_SH2_STAT5	341	344	PF00017	0.508
LIG_SH2_STAT5	419	422	PF00017	0.408
LIG_SH3_3	317	323	PF00018	0.457
LIG_SH3_3	563	569	PF00018	0.606
LIG_SH3_3	72	78	PF00018	0.408
LIG_SUMO_SIM_anti_2	434	441	PF11976	0.339
LIG_SUMO_SIM_anti_2	443	448	PF11976	0.330
LIG_TYR_ITIM	149	154	PF00017	0.516
LIG_TYR_ITIM	82	87	PF00017	0.417
LIG_WRC_WIRS_1	135	140	PF05994	0.438
LIG_WRC_WIRS_1	378	383	PF05994	0.428
LIG_WW_3	176	180	PF00397	0.441
LIG_WW_3	525	529	PF00397	0.461
MOD_CDC14_SPxK_1	620	623	PF00782	0.637
MOD_CDK_SPxK_1	124	130	PF00069	0.408
MOD_CDK_SPxK_1	617	623	PF00069	0.643
MOD_CDK_SPxK_1	74	80	PF00069	0.417
MOD_CDK_SPxxK_3	23	30	PF00069	0.229
MOD_CDK_SPxxK_3	460	467	PF00069	0.503
MOD_CDK_SPxxK_3	617	624	PF00069	0.618
MOD_CK1_1	211	217	PF00069	0.777
MOD_CK1_1	278	284	PF00069	0.658
MOD_CK1_1	287	293	PF00069	0.652
MOD_CK1_1	299	305	PF00069	0.604
MOD_CK1_1	364	370	PF00069	0.417
MOD_CK1_1	50	56	PF00069	0.430
MOD_CK1_1	562	568	PF00069	0.700
MOD_CK1_1	617	623	PF00069	0.676
MOD_CK2_1	193	199	PF00069	0.578
MOD_GlcNHglycan	210	213	PF01048	0.636
MOD_GlcNHglycan	277	280	PF01048	0.635
MOD_GlcNHglycan	289	292	PF01048	0.702
MOD_GlcNHglycan	468	471	PF01048	0.455
MOD_GlcNHglycan	479	482	PF01048	0.259
MOD_GlcNHglycan	49	52	PF01048	0.226
MOD_GlcNHglycan	536	539	PF01048	0.745
MOD_GlcNHglycan	540	543	PF01048	0.700
MOD_GlcNHglycan	552	555	PF01048	0.477
MOD_GlcNHglycan	576	579	PF01048	0.693
MOD_GlcNHglycan	585	588	PF01048	0.712
MOD_GSK3_1	11	18	PF00069	0.499
MOD_GSK3_1	207	214	PF00069	0.774
MOD_GSK3_1	218	225	PF00069	0.766
MOD_GSK3_1	251	258	PF00069	0.459
MOD_GSK3_1	285	292	PF00069	0.696
MOD_GSK3_1	456	463	PF00069	0.480
MOD_GSK3_1	534	541	PF00069	0.592
MOD_GSK3_1	543	550	PF00069	0.651
MOD_LATS_1	192	198	PF00433	0.642
MOD_LATS_1	332	338	PF00433	0.350
MOD_N-GLC_2	343	345	PF02516	0.217
MOD_NEK2_1	134	139	PF00069	0.397
MOD_NEK2_1	185	190	PF00069	0.409
MOD_NEK2_1	383	388	PF00069	0.505
MOD_NEK2_1	559	564	PF00069	0.720
MOD_PIKK_1	109	115	PF00454	0.452
MOD_PIKK_1	292	298	PF00454	0.495
MOD_PIKK_1	307	313	PF00454	0.368
MOD_PIKK_1	332	338	PF00454	0.438
MOD_PIKK_1	367	373	PF00454	0.428
MOD_PIKK_1	526	532	PF00454	0.465
MOD_PKA_2	158	164	PF00069	0.417
MOD_PKA_2	193	199	PF00069	0.576
MOD_PKA_2	351	357	PF00069	0.420
MOD_PKA_2	456	462	PF00069	0.355
MOD_PKA_2	521	527	PF00069	0.696
MOD_Plk_1	254	260	PF00069	0.551
MOD_Plk_1	442	448	PF00069	0.416
MOD_Plk_2-3	158	164	PF00069	0.486
MOD_Plk_4	15	21	PF00069	0.433
MOD_Plk_4	255	261	PF00069	0.422
MOD_Plk_4	335	341	PF00069	0.419
MOD_Plk_4	364	370	PF00069	0.424
MOD_Plk_4	402	408	PF00069	0.525
MOD_Plk_4	415	421	PF00069	0.327
MOD_Plk_4	442	448	PF00069	0.385
MOD_Plk_4	456	462	PF00069	0.317
MOD_ProDKin_1	124	130	PF00069	0.416
MOD_ProDKin_1	21	27	PF00069	0.366
MOD_ProDKin_1	218	224	PF00069	0.583
MOD_ProDKin_1	460	466	PF00069	0.500
MOD_ProDKin_1	571	577	PF00069	0.488
MOD_ProDKin_1	609	615	PF00069	0.737
MOD_ProDKin_1	617	623	PF00069	0.770
MOD_ProDKin_1	74	80	PF00069	0.417
TRG_DiLeu_BaEn_1	243	248	PF01217	0.497
TRG_DiLeu_BaLyEn_6	171	176	PF01217	0.514
TRG_DiLeu_BaLyEn_6	226	231	PF01217	0.482
TRG_DiLeu_BaLyEn_6	470	475	PF01217	0.482
TRG_ENDOCYTIC_2	151	154	PF00928	0.520
TRG_ENDOCYTIC_2	19	22	PF00928	0.394
TRG_ENDOCYTIC_2	313	316	PF00928	0.352
TRG_ENDOCYTIC_2	69	72	PF00928	0.418
TRG_ENDOCYTIC_2	84	87	PF00928	0.411
TRG_ER_diArg_1	182	184	PF00400	0.408
TRG_ER_diArg_1	205	207	PF00400	0.689
TRG_ER_diArg_1	531	534	PF00400	0.665
TRG_ER_diArg_1	555	558	PF00400	0.561
TRG_ER_diArg_1	623	626	PF00400	0.772
TRG_ER_diLys_1	627	630	PF00400	0.508
TRG_NLS_Bipartite_1	610	628	PF00514	0.624
TRG_NLS_MonoExtC_3	622	627	PF00514	0.616
TRG_NLS_MonoExtN_4	621	628	PF00514	0.736
TRG_Pf-PMV_PEXEL_1	132	136	PF00026	0.287
TRG_Pf-PMV_PEXEL_1	150	155	PF00026	0.175
TRG_Pf-PMV_PEXEL_1	80	85	PF00026	0.208

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1U1	Leptomonas seymouri	26%	81%
A0A0N1I2T9	Leptomonas seymouri	70%	100%
A0A0S4ISP4	Bodo saltans	27%	85%
A0A0S4J5D6	Bodo saltans	49%	100%
A0A0S4KKU4	Bodo saltans	29%	91%
A0A1X0NVK0	Trypanosomatidae	27%	88%
A0A1X0P0F9	Trypanosomatidae	55%	100%
A0A3Q8IE53	Leishmania donovani	27%	80%
A0A3R7M1K3	Trypanosoma rangeli	27%	88%
A0A3R7N587	Trypanosoma rangeli	26%	76%
A0A3S5H5H4	Leishmania donovani	79%	100%
A0A3S5H6T7	Leishmania donovani	31%	100%
A0A3S7X5R1	Leishmania donovani	26%	82%
A0A422NDK5	Trypanosoma rangeli	57%	100%
A2XVF7	Oryza sativa subsp. indica	31%	76%
A3AVH5	Oryza sativa subsp. japonica	31%	76%
A4HFC4	Leishmania braziliensis	27%	91%
A4HKL3	Leishmania braziliensis	27%	82%
A4HSF8	Leishmania infantum	80%	100%
A4I2K1	Leishmania infantum	27%	80%
A4I846	Leishmania infantum	26%	82%
A6ZL85	Saccharomyces cerevisiae (strain YJM789)	28%	82%
A7TJ71	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	28%	82%
C9ZNU6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	98%
D0A4H6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
D0A6Z3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	100%
D0AAB3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	87%
E9ACY2	Leishmania major	27%	80%
E9AKE2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	100%
E9AYQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	80%
E9B304	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	81%
O60173	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	89%
P26802	Drosophila melanogaster	30%	92%
P38112	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	82%
Q0CF43	Aspergillus terreus (strain NIH 2624 / FGSC A1156)	27%	82%
Q0UHM7	Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)	28%	76%
Q4IJH1	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	28%	100%
Q4Q552	Leishmania major	27%	82%
Q4QFH1	Leishmania major	31%	100%
Q4QJG6	Leishmania major	79%	99%
Q6C835	Yarrowia lipolytica (strain CLIB 122 / E 150)	29%	79%
Q6CRF4	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	29%	82%
Q6NZQ2	Mus musculus	26%	92%
Q7XJN0	Arabidopsis thaliana	26%	100%
Q93Y39	Arabidopsis thaliana	31%	76%
V5CZW7	Trypanosoma cruzi	57%	100%
V5DCA1	Trypanosoma cruzi	26%	88%