Mitotic spindle assembly checkpoint protein MAD1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania braziliensis

UniProt:

A4H451_LEIBR

TriTrypDb:

LbrM.05.0050 , LBRM2903_050005400

Length:

840

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	1
GO:0042995	cell projection	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0110165	cellular anatomical entity	1	2
GO:0120025	plasma membrane bounded cell projection	3	1

Expansion

Sequence features

A4H451

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H451

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006163	purine nucleotide metabolic process	5	11
GO:0006164	purine nucleotide biosynthetic process	6	11
GO:0006171	cAMP biosynthetic process	8	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006753	nucleoside phosphate metabolic process	4	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009117	nucleotide metabolic process	5	11
GO:0009150	purine ribonucleotide metabolic process	6	11
GO:0009152	purine ribonucleotide biosynthetic process	7	11
GO:0009165	nucleotide biosynthetic process	6	11
GO:0009187	cyclic nucleotide metabolic process	6	11
GO:0009190	cyclic nucleotide biosynthetic process	7	11
GO:0009259	ribonucleotide metabolic process	5	11
GO:0009260	ribonucleotide biosynthetic process	6	11
GO:0009987	cellular process	1	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019637	organophosphate metabolic process	3	11
GO:0019693	ribose phosphate metabolic process	4	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0044281	small molecule metabolic process	2	11
GO:0046058	cAMP metabolic process	7	11
GO:0046390	ribose phosphate biosynthetic process	5	11
GO:0046483	heterocycle metabolic process	3	11
GO:0052652	cyclic purine nucleotide metabolic process	6	11
GO:0055086	nucleobase-containing small molecule metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0072521	purine-containing compound metabolic process	4	11
GO:0072522	purine-containing compound biosynthetic process	5	11
GO:0090407	organophosphate biosynthetic process	4	11
GO:1901135	carbohydrate derivative metabolic process	3	11
GO:1901137	carbohydrate derivative biosynthetic process	4	11
GO:1901293	nucleoside phosphate biosynthetic process	5	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901566	organonitrogen compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11
GO:0007165	signal transduction	2	1
GO:0035556	intracellular signal transduction	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0065007	biological regulation	1	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	105	109	PF00656	0.523
CLV_C14_Caspase3-7	81	85	PF00656	0.636
CLV_NRD_NRD_1	112	114	PF00675	0.627
CLV_NRD_NRD_1	254	256	PF00675	0.605
CLV_NRD_NRD_1	424	426	PF00675	0.616
CLV_NRD_NRD_1	6	8	PF00675	0.524
CLV_NRD_NRD_1	600	602	PF00675	0.527
CLV_NRD_NRD_1	790	792	PF00675	0.626
CLV_PCSK_KEX2_1	112	114	PF00082	0.609
CLV_PCSK_KEX2_1	266	268	PF00082	0.643
CLV_PCSK_KEX2_1	424	426	PF00082	0.616
CLV_PCSK_KEX2_1	599	601	PF00082	0.559
CLV_PCSK_KEX2_1	790	792	PF00082	0.628
CLV_PCSK_PC1ET2_1	266	268	PF00082	0.643
CLV_PCSK_SKI1_1	122	126	PF00082	0.449
CLV_PCSK_SKI1_1	151	155	PF00082	0.504
CLV_PCSK_SKI1_1	24	28	PF00082	0.605
CLV_PCSK_SKI1_1	330	334	PF00082	0.593
CLV_PCSK_SKI1_1	763	767	PF00082	0.418
CLV_Separin_Metazoa	456	460	PF03568	0.562
CLV_Separin_Metazoa	66	70	PF03568	0.567
DEG_APCC_DBOX_1	600	608	PF00400	0.358
DEG_Nend_UBRbox_3	1	3	PF02207	0.783
DOC_CDC14_PxL_1	305	313	PF14671	0.581
DOC_CDC14_PxL_1	320	328	PF14671	0.588
DOC_CDC14_PxL_1	354	362	PF14671	0.429
DOC_CKS1_1	326	331	PF01111	0.416
DOC_CYCLIN_RxL_1	348	359	PF00134	0.509
DOC_CYCLIN_RxL_1	445	456	PF00134	0.327
DOC_CYCLIN_yClb5_NLxxxL_5	414	423	PF00134	0.652
DOC_CYCLIN_yClb5_NLxxxL_5	739	748	PF00134	0.356
DOC_CYCLIN_yClb5_NLxxxL_5	790	797	PF00134	0.395
DOC_CYCLIN_yCln2_LP_2	512	518	PF00134	0.464
DOC_CYCLIN_yCln2_LP_2	717	720	PF00134	0.573
DOC_MAPK_DCC_7	610	620	PF00069	0.528
DOC_MAPK_gen_1	266	274	PF00069	0.611
DOC_MAPK_gen_1	50	59	PF00069	0.644
DOC_MAPK_gen_1	537	545	PF00069	0.375
DOC_MAPK_gen_1	680	689	PF00069	0.561
DOC_MAPK_gen_1	805	815	PF00069	0.380
DOC_MAPK_MEF2A_6	267	276	PF00069	0.584
DOC_MAPK_MEF2A_6	435	444	PF00069	0.439
DOC_MAPK_MEF2A_6	808	815	PF00069	0.382
DOC_MAPK_NFAT4_5	808	816	PF00069	0.379
DOC_PP1_RVXF_1	175	182	PF00149	0.592
DOC_PP1_RVXF_1	409	415	PF00149	0.526
DOC_PP1_RVXF_1	433	440	PF00149	0.452
DOC_PP1_RVXF_1	446	452	PF00149	0.431
DOC_PP2B_LxvP_1	355	358	PF13499	0.383
DOC_PP2B_LxvP_1	442	445	PF13499	0.492
DOC_PP2B_LxvP_1	519	522	PF13499	0.543
DOC_PP2B_LxvP_1	717	720	PF13499	0.551
DOC_PP4_FxxP_1	36	39	PF00568	0.587
DOC_PP4_FxxP_1	647	650	PF00568	0.621
DOC_SPAK_OSR1_1	500	504	PF12202	0.300
DOC_USP7_MATH_1	127	131	PF00917	0.564
DOC_USP7_MATH_1	216	220	PF00917	0.596
DOC_USP7_MATH_1	3	7	PF00917	0.591
DOC_USP7_MATH_1	403	407	PF00917	0.469
DOC_USP7_MATH_1	44	48	PF00917	0.642
DOC_USP7_MATH_1	608	612	PF00917	0.476
DOC_USP7_MATH_1	616	620	PF00917	0.484
DOC_USP7_MATH_1	657	661	PF00917	0.699
DOC_USP7_UBL2_3	162	166	PF12436	0.622
DOC_WW_Pin1_4	325	330	PF00397	0.580
DOC_WW_Pin1_4	425	430	PF00397	0.516
DOC_WW_Pin1_4	653	658	PF00397	0.712
LIG_14-3-3_CanoR_1	151	160	PF00244	0.546
LIG_14-3-3_CanoR_1	232	237	PF00244	0.538
LIG_14-3-3_CanoR_1	267	275	PF00244	0.552
LIG_14-3-3_CanoR_1	459	463	PF00244	0.457
LIG_14-3-3_CanoR_1	540	544	PF00244	0.412
LIG_14-3-3_CanoR_1	7	13	PF00244	0.762
LIG_14-3-3_CanoR_1	707	713	PF00244	0.392
LIG_Actin_WH2_2	183	200	PF00022	0.591
LIG_Actin_WH2_2	458	474	PF00022	0.524
LIG_BIR_III_4	314	318	PF00653	0.420
LIG_deltaCOP1_diTrp_1	507	514	PF00928	0.452
LIG_FHA_1	185	191	PF00498	0.522
LIG_FHA_1	192	198	PF00498	0.530
LIG_FHA_1	25	31	PF00498	0.470
LIG_FHA_1	267	273	PF00498	0.535
LIG_FHA_1	407	413	PF00498	0.637
LIG_FHA_1	466	472	PF00498	0.561
LIG_FHA_1	54	60	PF00498	0.601
LIG_FHA_1	612	618	PF00498	0.524
LIG_FHA_1	7	13	PF00498	0.701
LIG_FHA_1	784	790	PF00498	0.628
LIG_FHA_2	124	130	PF00498	0.437
LIG_FHA_2	782	788	PF00498	0.577
LIG_FHA_2	834	840	PF00498	0.515
LIG_LIR_Apic_2	33	39	PF02991	0.585
LIG_LIR_Apic_2	549	554	PF02991	0.476
LIG_LIR_Apic_2	646	650	PF02991	0.613
LIG_LIR_Gen_1	729	739	PF02991	0.564
LIG_LIR_Nem_3	208	214	PF02991	0.648
LIG_LIR_Nem_3	298	304	PF02991	0.431
LIG_LIR_Nem_3	742	748	PF02991	0.586
LIG_LIR_Nem_3	767	771	PF02991	0.397
LIG_LIR_Nem_3	84	90	PF02991	0.528
LIG_LYPXL_SIV_4	346	354	PF13949	0.549
LIG_PTB_Apo_2	433	440	PF02174	0.491
LIG_PTB_Apo_2	562	569	PF02174	0.600
LIG_PTB_Phospho_1	562	568	PF10480	0.605
LIG_SH2_CRK	301	305	PF00017	0.327
LIG_SH2_CRK	466	470	PF00017	0.521
LIG_SH2_CRK	536	540	PF00017	0.458
LIG_SH2_PTP2	551	554	PF00017	0.588
LIG_SH2_SRC	551	554	PF00017	0.588
LIG_SH2_STAP1	247	251	PF00017	0.617
LIG_SH2_STAP1	482	486	PF00017	0.547
LIG_SH2_STAT3	247	250	PF00017	0.616
LIG_SH2_STAT5	22	25	PF00017	0.619
LIG_SH2_STAT5	292	295	PF00017	0.429
LIG_SH2_STAT5	327	330	PF00017	0.488
LIG_SH2_STAT5	413	416	PF00017	0.583
LIG_SH2_STAT5	551	554	PF00017	0.582
LIG_SH2_STAT5	768	771	PF00017	0.539
LIG_SH3_3	426	432	PF00018	0.506
LIG_SUMO_SIM_anti_2	268	276	PF11976	0.644
LIG_SUMO_SIM_anti_2	631	636	PF11976	0.450
LIG_SUMO_SIM_anti_2	685	693	PF11976	0.579
LIG_SUMO_SIM_par_1	353	359	PF11976	0.382
LIG_SUMO_SIM_par_1	55	61	PF11976	0.584
LIG_SUMO_SIM_par_1	685	693	PF11976	0.511
LIG_TRAF2_1	524	527	PF00917	0.514
LIG_TRAF2_1	754	757	PF00917	0.597
LIG_TRFH_1	715	719	PF08558	0.547
LIG_TYR_ITIM	464	469	PF00017	0.508
LIG_TYR_ITIM	534	539	PF00017	0.465
LIG_TYR_ITSM	297	304	PF00017	0.321
LIG_UBA3_1	354	362	PF00899	0.406
LIG_WRC_WIRS_1	161	166	PF05994	0.455
LIG_WRC_WIRS_1	297	302	PF05994	0.553
LIG_WRC_WIRS_1	405	410	PF05994	0.437
LIG_WRC_WIRS_1	617	622	PF05994	0.511
MOD_CDK_SPK_2	325	330	PF00069	0.416
MOD_CK1_1	265	271	PF00069	0.609
MOD_CK1_1	406	412	PF00069	0.498
MOD_CK1_1	53	59	PF00069	0.653
MOD_CK1_1	6	12	PF00069	0.596
MOD_CK1_1	611	617	PF00069	0.538
MOD_CK1_1	619	625	PF00069	0.614
MOD_CK1_1	656	662	PF00069	0.538
MOD_CK1_1	696	702	PF00069	0.593
MOD_CK1_1	831	837	PF00069	0.512
MOD_CK2_1	123	129	PF00069	0.518
MOD_CK2_1	134	140	PF00069	0.479
MOD_CK2_1	196	202	PF00069	0.582
MOD_CK2_1	242	248	PF00069	0.480
MOD_CK2_1	729	735	PF00069	0.553
MOD_CK2_1	781	787	PF00069	0.567
MOD_CK2_1	833	839	PF00069	0.501
MOD_CK2_1	88	94	PF00069	0.566
MOD_GlcNHglycan	372	375	PF01048	0.524
MOD_GlcNHglycan	46	49	PF01048	0.604
MOD_GlcNHglycan	52	55	PF01048	0.565
MOD_GlcNHglycan	526	531	PF01048	0.473
MOD_GlcNHglycan	553	557	PF01048	0.570
MOD_GlcNHglycan	623	626	PF01048	0.659
MOD_GlcNHglycan	773	777	PF01048	0.634
MOD_GSK3_1	123	130	PF00069	0.576
MOD_GSK3_1	196	203	PF00069	0.595
MOD_GSK3_1	2	9	PF00069	0.554
MOD_GSK3_1	262	269	PF00069	0.610
MOD_GSK3_1	288	295	PF00069	0.518
MOD_GSK3_1	403	410	PF00069	0.461
MOD_GSK3_1	480	487	PF00069	0.413
MOD_GSK3_1	653	660	PF00069	0.554
MOD_GSK3_1	69	76	PF00069	0.508
MOD_GSK3_1	693	700	PF00069	0.578
MOD_GSK3_1	718	725	PF00069	0.535
MOD_GSK3_1	829	836	PF00069	0.517
MOD_N-GLC_2	595	597	PF02516	0.516
MOD_NEK2_1	134	139	PF00069	0.485
MOD_NEK2_1	160	165	PF00069	0.568
MOD_NEK2_1	262	267	PF00069	0.594
MOD_NEK2_1	288	293	PF00069	0.530
MOD_NEK2_1	401	406	PF00069	0.584
MOD_NEK2_1	419	424	PF00069	0.685
MOD_NEK2_1	457	462	PF00069	0.452
MOD_NEK2_1	464	469	PF00069	0.398
MOD_NEK2_1	539	544	PF00069	0.413
MOD_NEK2_1	78	83	PF00069	0.619
MOD_NEK2_1	829	834	PF00069	0.560
MOD_PIKK_1	127	133	PF00454	0.505
MOD_PIKK_1	134	140	PF00454	0.449
MOD_PIKK_1	380	386	PF00454	0.581
MOD_PIKK_1	502	508	PF00454	0.377
MOD_PIKK_1	570	576	PF00454	0.423
MOD_PIKK_1	619	625	PF00454	0.641
MOD_PIKK_1	722	728	PF00454	0.511
MOD_PKA_1	266	272	PF00069	0.567
MOD_PKA_1	7	13	PF00069	0.691
MOD_PKA_2	200	206	PF00069	0.581
MOD_PKA_2	266	272	PF00069	0.631
MOD_PKA_2	3	9	PF00069	0.518
MOD_PKA_2	458	464	PF00069	0.453
MOD_PKA_2	539	545	PF00069	0.383
MOD_PKA_2	708	714	PF00069	0.530
MOD_Plk_1	191	197	PF00069	0.566
MOD_Plk_1	401	407	PF00069	0.532
MOD_Plk_1	419	425	PF00069	0.703
MOD_Plk_1	484	490	PF00069	0.490
MOD_Plk_1	88	94	PF00069	0.566
MOD_Plk_2-3	102	108	PF00069	0.360
MOD_Plk_2-3	191	197	PF00069	0.526
MOD_Plk_2-3	242	248	PF00069	0.521
MOD_Plk_2-3	480	486	PF00069	0.503
MOD_Plk_2-3	628	634	PF00069	0.430
MOD_Plk_2-3	643	649	PF00069	0.441
MOD_Plk_2-3	729	735	PF00069	0.585
MOD_Plk_2-3	781	787	PF00069	0.416
MOD_Plk_4	288	294	PF00069	0.483
MOD_Plk_4	296	302	PF00069	0.481
MOD_Plk_4	484	490	PF00069	0.393
MOD_Plk_4	664	670	PF00069	0.626
MOD_Plk_4	729	735	PF00069	0.550
MOD_ProDKin_1	325	331	PF00069	0.580
MOD_ProDKin_1	425	431	PF00069	0.509
MOD_ProDKin_1	653	659	PF00069	0.709
MOD_SUMO_for_1	146	149	PF00179	0.554
MOD_SUMO_rev_2	115	124	PF00179	0.511
MOD_SUMO_rev_2	169	174	PF00179	0.469
MOD_SUMO_rev_2	191	200	PF00179	0.561
MOD_SUMO_rev_2	259	268	PF00179	0.603
MOD_SUMO_rev_2	334	340	PF00179	0.567
TRG_DiLeu_BaEn_1	63	68	PF01217	0.523
TRG_DiLeu_BaEn_4	65	71	PF01217	0.629
TRG_DiLeu_BaLyEn_6	130	135	PF01217	0.504
TRG_ENDOCYTIC_2	211	214	PF00928	0.652
TRG_ENDOCYTIC_2	301	304	PF00928	0.433
TRG_ENDOCYTIC_2	466	469	PF00928	0.494
TRG_ENDOCYTIC_2	536	539	PF00928	0.456
TRG_ENDOCYTIC_2	731	734	PF00928	0.533
TRG_ENDOCYTIC_2	745	748	PF00928	0.481
TRG_ENDOCYTIC_2	768	771	PF00928	0.398
TRG_ENDOCYTIC_2	826	829	PF00928	0.490
TRG_ER_diArg_1	112	114	PF00400	0.627
TRG_ER_diArg_1	423	425	PF00400	0.619
TRG_ER_diArg_1	598	601	PF00400	0.554
TRG_ER_diArg_1	789	791	PF00400	0.602
TRG_NES_CRM1_1	630	643	PF08389	0.531
TRG_NES_CRM1_1	729	742	PF08389	0.585
TRG_Pf-PMV_PEXEL_1	133	138	PF00026	0.488
TRG_Pf-PMV_PEXEL_1	234	238	PF00026	0.611
TRG_Pf-PMV_PEXEL_1	389	393	PF00026	0.583
TRG_Pf-PMV_PEXEL_1	763	767	PF00026	0.405

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PC98	Leptomonas seymouri	68%	100%
A0A0S4JP59	Bodo saltans	30%	89%
A0A1X0P3L6	Trypanosomatidae	45%	100%
A0A3S5H5F6	Leishmania donovani	84%	100%
A0A422NYC2	Trypanosoma rangeli	46%	99%
A4HSC8	Leishmania infantum	85%	100%
C9ZPY3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	99%
E9AKA9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
Q4QJJ8	Leishmania major	85%	100%
V5BAC3	Trypanosoma cruzi	45%	99%