Adenylosuccinate lyase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

adenylosuccinate lyase, putative

Species:

Leishmania braziliensis

UniProt:

A4H3W5_LEIBR

TriTrypDb:

LbrM.04.0500 , LBRM2903_040010000

Length:

480

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H3W5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H3W5

PDB structure(s): 4mx2_A , 4mx2_B , 4mx2_C , 4mx2_D , 4mx2_E , 4mx2_F , 4mx2_G , 4mx2_H

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006163	purine nucleotide metabolic process	5	12
GO:0006164	purine nucleotide biosynthetic process	6	12
GO:0006167	AMP biosynthetic process	8	11
GO:0006188	IMP biosynthetic process	8	12
GO:0006189	'de novo' IMP biosynthetic process	9	11
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006753	nucleoside phosphate metabolic process	4	12
GO:0006793	phosphorus metabolic process	3	12
GO:0006796	phosphate-containing compound metabolic process	4	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009117	nucleotide metabolic process	5	12
GO:0009123	nucleoside monophosphate metabolic process	5	12
GO:0009124	nucleoside monophosphate biosynthetic process	6	12
GO:0009126	purine nucleoside monophosphate metabolic process	6	12
GO:0009127	purine nucleoside monophosphate biosynthetic process	7	12
GO:0009150	purine ribonucleotide metabolic process	6	12
GO:0009152	purine ribonucleotide biosynthetic process	7	12
GO:0009156	ribonucleoside monophosphate biosynthetic process	7	12
GO:0009161	ribonucleoside monophosphate metabolic process	6	12
GO:0009165	nucleotide biosynthetic process	6	12
GO:0009167	purine ribonucleoside monophosphate metabolic process	7	12
GO:0009168	purine ribonucleoside monophosphate biosynthetic process	8	12
GO:0009259	ribonucleotide metabolic process	5	12
GO:0009260	ribonucleotide biosynthetic process	6	12
GO:0009987	cellular process	1	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0019637	organophosphate metabolic process	3	12
GO:0019693	ribose phosphate metabolic process	4	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0044208	'de novo' AMP biosynthetic process	9	11
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0044281	small molecule metabolic process	2	12
GO:0046033	AMP metabolic process	7	11
GO:0046040	IMP metabolic process	7	12
GO:0046390	ribose phosphate biosynthetic process	5	12
GO:0046483	heterocycle metabolic process	3	12
GO:0055086	nucleobase-containing small molecule metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0072521	purine-containing compound metabolic process	4	12
GO:0072522	purine-containing compound biosynthetic process	5	12
GO:0090407	organophosphate biosynthetic process	4	12
GO:1901135	carbohydrate derivative metabolic process	3	12
GO:1901137	carbohydrate derivative biosynthetic process	4	12
GO:1901293	nucleoside phosphate biosynthetic process	5	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901566	organonitrogen compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004018	N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity	5	12
GO:0016829	lyase activity	2	12
GO:0016840	carbon-nitrogen lyase activity	3	12
GO:0016842	amidine-lyase activity	4	12
GO:0070626	(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity	5	11
GO:0000166	nucleotide binding	3	1
GO:0005488	binding	1	1
GO:0036094	small molecule binding	2	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901265	nucleoside phosphate binding	3	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	363	367	PF00656	0.355
CLV_NRD_NRD_1	267	269	PF00675	0.389
CLV_NRD_NRD_1	421	423	PF00675	0.355
CLV_PCSK_KEX2_1	267	269	PF00082	0.314
CLV_PCSK_KEX2_1	421	423	PF00082	0.355
CLV_PCSK_SKI1_1	163	167	PF00082	0.364
CLV_PCSK_SKI1_1	221	225	PF00082	0.358
CLV_PCSK_SKI1_1	362	366	PF00082	0.371
CLV_PCSK_SKI1_1	433	437	PF00082	0.355
CLV_PCSK_SKI1_1	84	88	PF00082	0.328
CLV_PCSK_SKI1_1	95	99	PF00082	0.328
DEG_APCC_DBOX_1	220	228	PF00400	0.528
DEG_APCC_DBOX_1	461	469	PF00400	0.355
DOC_CYCLIN_yClb5_NLxxxL_5	371	377	PF00134	0.476
DOC_CYCLIN_yCln2_LP_2	373	379	PF00134	0.355
DOC_MAPK_gen_1	41	49	PF00069	0.431
DOC_MAPK_gen_1	60	67	PF00069	0.514
DOC_MAPK_MEF2A_6	60	67	PF00069	0.514
DOC_PP2B_LxvP_1	225	228	PF13499	0.564
DOC_PP2B_LxvP_1	373	376	PF13499	0.355
DOC_USP7_MATH_1	383	387	PF00917	0.344
DOC_USP7_UBL2_3	118	122	PF12436	0.431
DOC_WW_Pin1_4	199	204	PF00397	0.503
DOC_WW_Pin1_4	27	32	PF00397	0.507
DOC_WW_Pin1_4	33	38	PF00397	0.470
DOC_WW_Pin1_4	466	471	PF00397	0.355
LIG_14-3-3_CanoR_1	268	278	PF00244	0.499
LIG_14-3-3_CanoR_1	41	49	PF00244	0.512
LIG_14-3-3_CanoR_1	50	59	PF00244	0.530
LIG_14-3-3_CanoR_1	84	91	PF00244	0.515
LIG_14-3-3_CanoR_1	95	100	PF00244	0.513
LIG_Actin_WH2_2	374	391	PF00022	0.459
LIG_Actin_WH2_2	70	86	PF00022	0.517
LIG_AP2alpha_1	255	259	PF02296	0.434
LIG_BIR_II_1	1	5	PF00653	0.750
LIG_BRCT_BRCA1_1	29	33	PF00533	0.361
LIG_deltaCOP1_diTrp_1	251	259	PF00928	0.461
LIG_FHA_1	10	16	PF00498	0.615
LIG_FHA_1	270	276	PF00498	0.509
LIG_FHA_1	44	50	PF00498	0.511
LIG_FHA_1	456	462	PF00498	0.495
LIG_FHA_2	101	107	PF00498	0.565
LIG_FHA_2	115	121	PF00498	0.395
LIG_FHA_2	137	143	PF00498	0.528
LIG_FHA_2	147	153	PF00498	0.506
LIG_FHA_2	271	277	PF00498	0.503
LIG_GBD_Chelix_1	412	420	PF00786	0.355
LIG_IRF3_LxIS_1	351	358	PF10401	0.419
LIG_LIR_Apic_2	187	191	PF02991	0.516
LIG_LIR_Apic_2	423	428	PF02991	0.355
LIG_LIR_Gen_1	140	148	PF02991	0.517
LIG_LIR_Gen_1	276	284	PF02991	0.514
LIG_LIR_Gen_1	301	311	PF02991	0.503
LIG_LIR_Gen_1	406	416	PF02991	0.342
LIG_LIR_Gen_1	469	476	PF02991	0.360
LIG_LIR_Gen_1	54	63	PF02991	0.528
LIG_LIR_Gen_1	98	107	PF02991	0.588
LIG_LIR_Nem_3	140	146	PF02991	0.516
LIG_LIR_Nem_3	251	255	PF02991	0.512
LIG_LIR_Nem_3	261	266	PF02991	0.545
LIG_LIR_Nem_3	276	281	PF02991	0.388
LIG_LIR_Nem_3	301	306	PF02991	0.503
LIG_LIR_Nem_3	406	412	PF02991	0.342
LIG_LIR_Nem_3	469	474	PF02991	0.347
LIG_LIR_Nem_3	54	59	PF02991	0.514
LIG_LIR_Nem_3	98	102	PF02991	0.588
LIG_LYPXL_yS_3	247	250	PF13949	0.503
LIG_NRBOX	176	182	PF00104	0.583
LIG_Pex14_2	255	259	PF04695	0.503
LIG_SH2_CRK	278	282	PF00017	0.528
LIG_SH2_GRB2like	278	281	PF00017	0.543
LIG_SH2_GRB2like	310	313	PF00017	0.431
LIG_SH2_PTP2	305	308	PF00017	0.608
LIG_SH2_SRC	170	173	PF00017	0.426
LIG_SH2_STAP1	271	275	PF00017	0.513
LIG_SH2_STAP1	278	282	PF00017	0.488
LIG_SH2_STAP1	476	480	PF00017	0.514
LIG_SH2_STAT3	271	274	PF00017	0.528
LIG_SH2_STAT3	310	313	PF00017	0.564
LIG_SH2_STAT3	61	64	PF00017	0.589
LIG_SH2_STAT5	127	130	PF00017	0.517
LIG_SH2_STAT5	170	173	PF00017	0.494
LIG_SH2_STAT5	263	266	PF00017	0.508
LIG_SH2_STAT5	271	274	PF00017	0.484
LIG_SH2_STAT5	305	308	PF00017	0.503
LIG_SH2_STAT5	310	313	PF00017	0.503
LIG_SH2_STAT5	473	476	PF00017	0.421
LIG_SH2_STAT5	52	55	PF00017	0.528
LIG_SH2_STAT5	69	72	PF00017	0.514
LIG_SH3_3	197	203	PF00018	0.503
LIG_SH3_3	393	399	PF00018	0.255
LIG_SUMO_SIM_par_1	464	469	PF11976	0.372
LIG_TRAF2_1	5	8	PF00917	0.759
LIG_TRFH_1	33	37	PF08558	0.490
LIG_UBA3_1	177	184	PF00899	0.528
LIG_UBA3_1	223	232	PF00899	0.565
LIG_UBA3_1	294	300	PF00899	0.565
LIG_WRC_WIRS_1	447	452	PF05994	0.390
MOD_CK1_1	2	8	PF00069	0.746
MOD_CK1_1	27	33	PF00069	0.589
MOD_CK2_1	136	142	PF00069	0.431
MOD_CK2_1	2	8	PF00069	0.762
MOD_CK2_1	270	276	PF00069	0.503
MOD_GlcNHglycan	132	135	PF01048	0.408
MOD_GlcNHglycan	22	25	PF01048	0.625
MOD_GlcNHglycan	322	325	PF01048	0.314
MOD_GlcNHglycan	351	354	PF01048	0.482
MOD_GlcNHglycan	4	7	PF01048	0.739
MOD_GSK3_1	20	27	PF00069	0.508
MOD_GSK3_1	223	230	PF00069	0.604
MOD_GSK3_1	9	16	PF00069	0.604
MOD_N-GLC_1	100	105	PF02516	0.402
MOD_N-GLC_1	153	158	PF02516	0.307
MOD_NEK2_1	1	6	PF00069	0.501
MOD_NEK2_1	146	151	PF00069	0.514
MOD_NEK2_1	180	185	PF00069	0.547
MOD_NEK2_1	193	198	PF00069	0.488
MOD_NEK2_1	223	228	PF00069	0.608
MOD_NEK2_1	258	263	PF00069	0.552
MOD_NEK2_1	341	346	PF00069	0.440
MOD_NEK2_1	364	369	PF00069	0.378
MOD_PIKK_1	147	153	PF00454	0.514
MOD_PIKK_1	270	276	PF00454	0.503
MOD_PIKK_1	78	84	PF00454	0.608
MOD_PKA_2	269	275	PF00069	0.592
MOD_PKB_1	268	276	PF00069	0.608
MOD_Plk_1	100	106	PF00069	0.555
MOD_Plk_1	153	159	PF00069	0.502
MOD_Plk_1	365	371	PF00069	0.355
MOD_Plk_2-3	100	106	PF00069	0.577
MOD_Plk_2-3	455	461	PF00069	0.502
MOD_Plk_4	290	296	PF00069	0.608
MOD_Plk_4	365	371	PF00069	0.370
MOD_Plk_4	383	389	PF00069	0.298
MOD_Plk_4	415	421	PF00069	0.355
MOD_ProDKin_1	199	205	PF00069	0.503
MOD_ProDKin_1	27	33	PF00069	0.504
MOD_ProDKin_1	466	472	PF00069	0.355
MOD_SUMO_rev_2	114	124	PF00179	0.514
TRG_DiLeu_BaEn_3	218	224	PF01217	0.608
TRG_ENDOCYTIC_2	247	250	PF00928	0.503
TRG_ENDOCYTIC_2	263	266	PF00928	0.514
TRG_ENDOCYTIC_2	278	281	PF00928	0.424
TRG_ENDOCYTIC_2	305	308	PF00928	0.503
TRG_ENDOCYTIC_2	429	432	PF00928	0.355
TRG_ENDOCYTIC_2	56	59	PF00928	0.514
TRG_ER_diArg_1	266	268	PF00400	0.589
TRG_ER_diArg_1	420	422	PF00400	0.355
TRG_Pf-PMV_PEXEL_1	362	366	PF00026	0.355

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0H3AL67	Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)	49%	100%
A0A0N1I0W7	Leptomonas seymouri	83%	100%
A0A0S4JC64	Bodo saltans	71%	100%
A0A1X0NMR7	Trypanosomatidae	72%	100%
A0A3R7N1M2	Trypanosoma rangeli	74%	100%
A3KN12	Bos taurus	24%	98%
A4HS40	Leishmania infantum	91%	100%
A5IGB3	Legionella pneumophila (strain Corby)	48%	100%
A7LBL3	Leishmania donovani	91%	100%
C9ZY37	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	70%	100%
E9AK27	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	99%
O27580	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	29%	100%
O28041	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	31%	100%
O58582	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	25%	100%
O66856	Aquifex aeolicus (strain VF5)	27%	100%
P0AB89	Escherichia coli (strain K12)	50%	100%
P0AB90	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	50%	100%
P12047	Bacillus subtilis (strain 168)	25%	100%
P30566	Homo sapiens	23%	99%
P44797	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	49%	100%
P54822	Mus musculus	23%	99%
P57351	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	43%	100%
P72478	Streptococcus mutans serotype c (strain ATCC 700610 / UA159)	27%	100%
Q05911	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q2FFI7	Staphylococcus aureus (strain USA300)	24%	100%
Q2G2S0	Staphylococcus aureus (strain NCTC 8325 / PS 47)	24%	100%
Q2YU66	Staphylococcus aureus (strain bovine RF122 / ET3-1)	24%	100%
Q49YV3	Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41)	24%	100%
Q4L7M3	Staphylococcus haemolyticus (strain JCSC1435)	25%	100%
Q54J34	Dictyostelium discoideum	56%	100%
Q58339	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	27%	100%
Q5HEL4	Staphylococcus aureus (strain COL)	24%	100%
Q5HN26	Staphylococcus epidermidis (strain ATCC 35984 / RP62A)	24%	100%
Q5ZXD1	Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)	48%	100%
Q6G825	Staphylococcus aureus (strain MSSA476)	24%	100%
Q6GFE9	Staphylococcus aureus (strain MRSA252)	24%	100%
Q7A0G9	Staphylococcus aureus (strain MW2)	24%	100%
Q7A4Q3	Staphylococcus aureus (strain N315)	24%	100%
Q89AM3	Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)	42%	100%
Q8CRT6	Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)	24%	100%
Q8HXY5	Macaca fascicularis	24%	99%
Q8K9Q7	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	42%	100%
Q99SX9	Staphylococcus aureus (strain Mu50 / ATCC 700699)	24%	100%
Q9I0K9	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	50%	100%
Q9RSE6	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	27%	100%
Q9U0T7	Leishmania major	90%	100%
Q9UZ99	Pyrococcus abyssi (strain GE5 / Orsay)	26%	100%
Q9X0I0	Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)	27%	100%
Q9ZKA2	Helicobacter pylori (strain J99 / ATCC 700824)	25%	100%
V5BE01	Trypanosoma cruzi	72%	100%