Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteine peptidase, Clan CA, family C2, putative

Species:

Leishmania braziliensis

UniProt:

A4H3W4_LEIBR

TriTrypDb:

LbrM.04.0490 , LBRM2903_040009900

Length:

856

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 38

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 103
NetGPI	no	yes: 0, no: 105

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	5
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0005930	axoneme	2	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A4H3W4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H3W4

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	106
GO:0006807	nitrogen compound metabolic process	2	106
GO:0008152	metabolic process	1	106
GO:0019538	protein metabolic process	3	106
GO:0043170	macromolecule metabolic process	3	106
GO:0044238	primary metabolic process	2	106
GO:0071704	organic substance metabolic process	2	106
GO:1901564	organonitrogen compound metabolic process	3	106

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	106
GO:0004175	endopeptidase activity	4	106
GO:0004197	cysteine-type endopeptidase activity	5	106
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	106
GO:0008233	peptidase activity	3	106
GO:0008234	cysteine-type peptidase activity	4	106
GO:0016787	hydrolase activity	2	106
GO:0140096	catalytic activity, acting on a protein	2	106

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	275	279	PF00656	0.437
CLV_C14_Caspase3-7	303	307	PF00656	0.528
CLV_NRD_NRD_1	383	385	PF00675	0.256
CLV_NRD_NRD_1	86	88	PF00675	0.543
CLV_PCSK_KEX2_1	383	385	PF00082	0.264
CLV_PCSK_SKI1_1	253	257	PF00082	0.520
CLV_PCSK_SKI1_1	311	315	PF00082	0.255
CLV_PCSK_SKI1_1	402	406	PF00082	0.324
CLV_PCSK_SKI1_1	88	92	PF00082	0.649
CLV_Separin_Metazoa	44	48	PF03568	0.488
DEG_APCC_KENBOX_2	474	478	PF00400	0.363
DEG_Kelch_Keap1_1	208	213	PF01344	0.376
DEG_SCF_TRCP1_1	306	312	PF00400	0.363
DOC_ANK_TNKS_1	383	390	PF00023	0.454
DOC_CDC14_PxL_1	736	744	PF14671	0.399
DOC_CKS1_1	498	503	PF01111	0.471
DOC_CKS1_1	572	577	PF01111	0.476
DOC_CYCLIN_RxL_1	238	248	PF00134	0.283
DOC_CYCLIN_yClb1_LxF_4	288	294	PF00134	0.428
DOC_CYCLIN_yCln2_LP_2	556	562	PF00134	0.548
DOC_CYCLIN_yCln2_LP_2	762	768	PF00134	0.454
DOC_MAPK_DCC_7	760	768	PF00069	0.429
DOC_MAPK_gen_1	491	498	PF00069	0.454
DOC_MAPK_gen_1	591	598	PF00069	0.496
DOC_MAPK_MEF2A_6	259	266	PF00069	0.347
DOC_MAPK_MEF2A_6	645	652	PF00069	0.402
DOC_MAPK_MEF2A_6	785	793	PF00069	0.468
DOC_PP1_RVXF_1	137	143	PF00149	0.649
DOC_PP1_RVXF_1	390	396	PF00149	0.479
DOC_PP1_RVXF_1	483	490	PF00149	0.533
DOC_PP2B_LxvP_1	102	105	PF13499	0.525
DOC_PP2B_LxvP_1	686	689	PF13499	0.256
DOC_PP2B_LxvP_1	737	740	PF13499	0.381
DOC_PP4_FxxP_1	157	160	PF00568	0.304
DOC_PP4_FxxP_1	373	376	PF00568	0.482
DOC_PP4_FxxP_1	617	620	PF00568	0.393
DOC_PP4_FxxP_1	83	86	PF00568	0.545
DOC_USP7_MATH_1	300	304	PF00917	0.466
DOC_USP7_MATH_1	309	313	PF00917	0.480
DOC_USP7_MATH_1	777	781	PF00917	0.611
DOC_USP7_UBL2_3	435	439	PF12436	0.476
DOC_USP7_UBL2_3	544	548	PF12436	0.545
DOC_USP7_UBL2_3	88	92	PF12436	0.543
DOC_WW_Pin1_4	122	127	PF00397	0.616
DOC_WW_Pin1_4	497	502	PF00397	0.485
DOC_WW_Pin1_4	511	516	PF00397	0.479
DOC_WW_Pin1_4	571	576	PF00397	0.514
DOC_WW_Pin1_4	90	95	PF00397	0.429
LIG_14-3-3_CanoR_1	253	262	PF00244	0.514
LIG_14-3-3_CanoR_1	392	396	PF00244	0.462
LIG_14-3-3_CanoR_1	626	632	PF00244	0.418
LIG_14-3-3_CanoR_1	701	710	PF00244	0.396
LIG_14-3-3_CanoR_1	87	94	PF00244	0.604
LIG_BIR_II_1	1	5	PF00653	0.749
LIG_BRCT_BRCA1_1	179	183	PF00533	0.315
LIG_BRCT_BRCA1_1	19	23	PF00533	0.668
LIG_BRCT_BRCA1_1	309	313	PF00533	0.513
LIG_BRCT_BRCA1_1	513	517	PF00533	0.601
LIG_BRCT_BRCA1_1	772	776	PF00533	0.300
LIG_CSL_BTD_1	96	99	PF09270	0.340
LIG_deltaCOP1_diTrp_1	712	717	PF00928	0.451
LIG_FHA_1	528	534	PF00498	0.485
LIG_FHA_1	6	12	PF00498	0.569
LIG_FHA_1	649	655	PF00498	0.466
LIG_FHA_1	724	730	PF00498	0.483
LIG_FHA_1	782	788	PF00498	0.496
LIG_FHA_1	823	829	PF00498	0.453
LIG_FHA_2	196	202	PF00498	0.338
LIG_FHA_2	204	210	PF00498	0.210
LIG_FHA_2	275	281	PF00498	0.432
LIG_FHA_2	359	365	PF00498	0.437
LIG_FHA_2	53	59	PF00498	0.580
LIG_FHA_2	628	634	PF00498	0.424
LIG_FHA_2	701	707	PF00498	0.304
LIG_LIR_Apic_2	154	160	PF02991	0.297
LIG_LIR_Apic_2	80	86	PF02991	0.594
LIG_LIR_Gen_1	434	445	PF02991	0.482
LIG_LIR_Gen_1	597	607	PF02991	0.490
LIG_LIR_Gen_1	712	723	PF02991	0.434
LIG_LIR_Gen_1	733	743	PF02991	0.358
LIG_LIR_Gen_1	771	779	PF02991	0.333
LIG_LIR_Nem_3	295	301	PF02991	0.439
LIG_LIR_Nem_3	310	316	PF02991	0.502
LIG_LIR_Nem_3	407	413	PF02991	0.503
LIG_LIR_Nem_3	427	432	PF02991	0.473
LIG_LIR_Nem_3	443	447	PF02991	0.469
LIG_LIR_Nem_3	597	603	PF02991	0.477
LIG_LIR_Nem_3	678	682	PF02991	0.448
LIG_LIR_Nem_3	694	700	PF02991	0.415
LIG_LIR_Nem_3	712	718	PF02991	0.448
LIG_LIR_Nem_3	733	739	PF02991	0.345
LIG_LIR_Nem_3	771	775	PF02991	0.312
LIG_LIR_Nem_3	818	823	PF02991	0.515
LIG_LIR_Nem_3	96	100	PF02991	0.486
LIG_LYPXL_yS_3	679	682	PF13949	0.370
LIG_PDZ_Class_2	851	856	PF00595	0.342
LIG_Pex14_2	228	232	PF04695	0.361
LIG_PTB_Apo_2	335	342	PF02174	0.364
LIG_PTB_Phospho_1	335	341	PF10480	0.363
LIG_SH2_CRK	444	448	PF00017	0.472
LIG_SH2_CRK	494	498	PF00017	0.494
LIG_SH2_CRK	697	701	PF00017	0.538
LIG_SH2_NCK_1	123	127	PF00017	0.672
LIG_SH2_NCK_1	444	448	PF00017	0.494
LIG_SH2_PTP2	324	327	PF00017	0.524
LIG_SH2_STAP1	772	776	PF00017	0.300
LIG_SH2_STAT5	214	217	PF00017	0.365
LIG_SH2_STAT5	249	252	PF00017	0.431
LIG_SH2_STAT5	324	327	PF00017	0.462
LIG_SH2_STAT5	409	412	PF00017	0.480
LIG_SH2_STAT5	609	612	PF00017	0.501
LIG_SH2_STAT5	736	739	PF00017	0.374
LIG_SH2_STAT5	836	839	PF00017	0.494
LIG_SH3_3	164	170	PF00018	0.504
LIG_SH3_3	61	67	PF00018	0.742
LIG_SH3_3	622	628	PF00018	0.213
LIG_SH3_3	641	647	PF00018	0.401
LIG_SH3_3	68	74	PF00018	0.706
LIG_SH3_3	818	824	PF00018	0.504
LIG_SH3_4	326	333	PF00018	0.441
LIG_SUMO_SIM_anti_2	595	600	PF11976	0.480
LIG_SUMO_SIM_par_1	213	219	PF11976	0.366
LIG_SUMO_SIM_par_1	539	545	PF11976	0.571
LIG_SUMO_SIM_par_1	680	685	PF11976	0.406
LIG_TRAF2_1	67	70	PF00917	0.815
LIG_TRAF2_1	795	798	PF00917	0.274
LIG_TRAF2_1	830	833	PF00917	0.555
LIG_TRFH_1	736	740	PF08558	0.373
LIG_TYR_ITIM	162	167	PF00017	0.344
LIG_UBA3_1	486	492	PF00899	0.483
LIG_WRC_WIRS_1	173	178	PF05994	0.359
LIG_WRC_WIRS_1	188	193	PF05994	0.415
MOD_CDK_SPxK_1	497	503	PF00069	0.492
MOD_CK1_1	12	18	PF00069	0.638
MOD_CK1_1	195	201	PF00069	0.327
MOD_CK1_1	304	310	PF00069	0.484
MOD_CK1_1	564	570	PF00069	0.542
MOD_CK1_1	780	786	PF00069	0.633
MOD_CK1_1	93	99	PF00069	0.432
MOD_CK2_1	195	201	PF00069	0.307
MOD_CK2_1	274	280	PF00069	0.449
MOD_CK2_1	358	364	PF00069	0.476
MOD_CK2_1	780	786	PF00069	0.404
MOD_CK2_1	827	833	PF00069	0.507
MOD_GlcNHglycan	208	213	PF01048	0.334
MOD_GlcNHglycan	30	33	PF01048	0.519
MOD_GlcNHglycan	306	309	PF01048	0.289
MOD_GlcNHglycan	448	451	PF01048	0.280
MOD_GlcNHglycan	476	480	PF01048	0.266
MOD_GlcNHglycan	563	566	PF01048	0.336
MOD_GlcNHglycan	810	813	PF01048	0.435
MOD_GSK3_1	192	199	PF00069	0.378
MOD_GSK3_1	249	256	PF00069	0.399
MOD_GSK3_1	300	307	PF00069	0.482
MOD_GSK3_1	457	464	PF00069	0.474
MOD_GSK3_1	5	12	PF00069	0.662
MOD_GSK3_1	511	518	PF00069	0.483
MOD_GSK3_1	648	655	PF00069	0.451
MOD_GSK3_1	701	708	PF00069	0.407
MOD_GSK3_1	777	784	PF00069	0.580
MOD_GSK3_1	86	93	PF00069	0.505
MOD_LATS_1	194	200	PF00433	0.166
MOD_N-GLC_1	12	17	PF02516	0.639
MOD_N-GLC_1	177	182	PF02516	0.323
MOD_N-GLC_1	701	706	PF02516	0.432
MOD_NEK2_1	17	22	PF00069	0.638
MOD_NEK2_1	200	205	PF00069	0.277
MOD_NEK2_1	400	405	PF00069	0.501
MOD_NEK2_1	440	445	PF00069	0.490
MOD_NEK2_1	527	532	PF00069	0.478
MOD_NEK2_1	640	645	PF00069	0.393
MOD_NEK2_1	648	653	PF00069	0.364
MOD_NEK2_2	309	314	PF00069	0.530
MOD_PIKK_1	243	249	PF00454	0.449
MOD_PIKK_1	253	259	PF00454	0.478
MOD_PIKK_1	326	332	PF00454	0.540
MOD_PIKK_1	358	364	PF00454	0.552
MOD_PIKK_1	564	570	PF00454	0.499
MOD_PIKK_1	705	711	PF00454	0.493
MOD_PIKK_1	827	833	PF00454	0.527
MOD_PKA_2	391	397	PF00069	0.455
MOD_PKA_2	700	706	PF00069	0.496
MOD_PKA_2	86	92	PF00069	0.535
MOD_Plk_1	12	18	PF00069	0.685
MOD_Plk_1	216	222	PF00069	0.354
MOD_Plk_1	294	300	PF00069	0.476
MOD_Plk_1	594	600	PF00069	0.459
MOD_Plk_1	648	654	PF00069	0.380
MOD_Plk_1	745	751	PF00069	0.486
MOD_Plk_1	770	776	PF00069	0.290
MOD_Plk_2-3	179	185	PF00069	0.277
MOD_Plk_2-3	34	40	PF00069	0.674
MOD_Plk_4	12	18	PF00069	0.619
MOD_Plk_4	216	222	PF00069	0.337
MOD_Plk_4	34	40	PF00069	0.652
MOD_Plk_4	536	542	PF00069	0.498
MOD_Plk_4	594	600	PF00069	0.481
MOD_Plk_4	635	641	PF00069	0.404
MOD_Plk_4	648	654	PF00069	0.372
MOD_Plk_4	93	99	PF00069	0.474
MOD_ProDKin_1	122	128	PF00069	0.626
MOD_ProDKin_1	497	503	PF00069	0.485
MOD_ProDKin_1	511	517	PF00069	0.479
MOD_ProDKin_1	571	577	PF00069	0.514
MOD_ProDKin_1	90	96	PF00069	0.428
MOD_SUMO_for_1	325	328	PF00179	0.548
MOD_SUMO_rev_2	478	487	PF00179	0.554
MOD_SUMO_rev_2	539	546	PF00179	0.526
TRG_DiLeu_BaEn_1	158	163	PF01217	0.331
TRG_DiLeu_BaEn_2	223	229	PF01217	0.255
TRG_DiLeu_BaEn_4	158	164	PF01217	0.336
TRG_ENDOCYTIC_2	164	167	PF00928	0.319
TRG_ENDOCYTIC_2	409	412	PF00928	0.499
TRG_ENDOCYTIC_2	444	447	PF00928	0.474
TRG_ENDOCYTIC_2	494	497	PF00928	0.483
TRG_ENDOCYTIC_2	538	541	PF00928	0.501
TRG_ENDOCYTIC_2	679	682	PF00928	0.406
TRG_ENDOCYTIC_2	697	700	PF00928	0.269
TRG_ENDOCYTIC_2	736	739	PF00928	0.356
TRG_ENDOCYTIC_2	772	775	PF00928	0.318
TRG_ER_diArg_1	320	323	PF00400	0.503
TRG_Pf-PMV_PEXEL_1	423	427	PF00026	0.290

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	30%	100%
A0A0N1I8N2	Leptomonas seymouri	29%	87%
A0A0N1IGQ2	Leptomonas seymouri	30%	100%
A0A0N1ILF1	Leptomonas seymouri	35%	100%
A0A0N1IMH1	Leptomonas seymouri	31%	100%
A0A0N1P9P1	Leptomonas seymouri	72%	100%
A0A0N1PCA9	Leptomonas seymouri	31%	100%
A0A0N1PE91	Leptomonas seymouri	26%	96%
A0A0N1PFI4	Leptomonas seymouri	36%	100%
A0A0S4JLK6	Bodo saltans	28%	100%
A0A0S4JS70	Bodo saltans	26%	100%
A0A0S4KGT2	Bodo saltans	38%	100%
A0A1X0NJ19	Trypanosomatidae	27%	70%
A0A1X0NJK2	Trypanosomatidae	34%	100%
A0A1X0NJX8	Trypanosomatidae	31%	100%
A0A1X0NKT7	Trypanosomatidae	32%	100%
A0A1X0NKX8	Trypanosomatidae	31%	100%
A0A1X0NMT3	Trypanosomatidae	48%	100%
A0A1X0NW84	Trypanosomatidae	36%	100%
A0A1X0NW85	Trypanosomatidae	36%	100%
A0A1X0NW89	Trypanosomatidae	43%	99%
A0A1X0NWA6	Trypanosomatidae	28%	98%
A0A1X0NWW1	Trypanosomatidae	39%	100%
A0A3Q8IBS3	Leishmania donovani	41%	94%
A0A3Q8IDD4	Leishmania donovani	31%	100%
A0A3Q8IJT4	Leishmania donovani	27%	100%
A0A3S5H5A5	Leishmania donovani	85%	100%
A0A3S5ISG2	Trypanosoma rangeli	32%	100%
A0A3S7WW13	Leishmania donovani	28%	77%
A0A3S7WW18	Leishmania donovani	40%	100%
A0A3S7WW41	Leishmania donovani	30%	100%
A0A3S7WW71	Leishmania donovani	34%	100%
A0A3S7X430	Leishmania donovani	31%	100%
A0A3S7X438	Leishmania donovani	33%	91%
A0A3S7X460	Leishmania donovani	30%	100%
A0A3S7X463	Leishmania donovani	30%	91%
A0A3S7X470	Leishmania donovani	33%	100%
A0A422MYU1	Trypanosoma rangeli	43%	93%
A0A422MYX0	Trypanosoma rangeli	36%	100%
A4HE81	Leishmania braziliensis	30%	100%
A4HJ14	Leishmania braziliensis	31%	100%
A4HJ21	Leishmania braziliensis	29%	96%
A4HJ22	Leishmania braziliensis	31%	90%
A4HJ24	Leishmania braziliensis	33%	100%
A4HS39	Leishmania infantum	85%	100%
A4HYN0	Leishmania infantum	40%	100%
A4HYW1	Leishmania infantum	41%	84%
A4HYW2	Leishmania infantum	34%	100%
A4HYW3	Leishmania infantum	30%	100%
A4HYW4	Leishmania infantum	28%	86%
A4I1J4	Leishmania infantum	31%	100%
A4I6E4	Leishmania infantum	33%	100%
A4I6E6	Leishmania infantum	30%	100%
A4I6F0	Leishmania infantum	33%	91%
A4I6K4	Leishmania infantum	31%	100%
A4I6K5	Leishmania infantum	30%	91%
A4I6K6	Leishmania infantum	27%	100%
C9ZIE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	79%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	91%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AIH1	Leishmania braziliensis	40%	100%
E9AIH3	Leishmania braziliensis	34%	100%
E9AIH4	Leishmania braziliensis	30%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	92%
E9AUQ8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	91%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
Q4Q6L7	Leishmania major	30%	100%
Q4Q6L9	Leishmania major	33%	100%
Q4Q6M0	Leishmania major	33%	100%
Q4Q6M2	Leishmania major	27%	100%
Q4Q6M3	Leishmania major	29%	100%
Q4Q6M4	Leishmania major	33%	100%
Q4Q9U3	Leishmania major	31%	98%
Q4QCS6	Leishmania major	30%	100%
Q4QCS7	Leishmania major	33%	91%
Q4QCS8	Leishmania major	40%	100%
Q4QCS9	Leishmania major	41%	100%
Q9U0T9	Leishmania major	84%	100%
V5AYJ1	Trypanosoma cruzi	30%	100%
V5B5I4	Trypanosoma cruzi	43%	100%
V5BA05	Trypanosoma cruzi	40%	100%
V5BEL3	Trypanosoma cruzi	36%	100%
V5BII7	Trypanosoma cruzi	28%	69%
V5BN20	Trypanosoma cruzi	31%	100%
V5D5V8	Trypanosoma cruzi	29%	100%
V5D9Y2	Trypanosoma cruzi	46%	100%
V5DES7	Trypanosoma cruzi	32%	100%