Putative elongation initiation factor 2 alpha subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

eukaryotic translation initiation factor 2 subunit alpha

Species:

Leishmania braziliensis

UniProt:

A4H3R0_LEIBR

TriTrypDb:

LbrM.03.0890 , LBRM2903_030014900

Length:

413

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 6

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0005737	cytoplasm	2	1
GO:0005850	eukaryotic translation initiation factor 2 complex	2	1
GO:0005851	eukaryotic translation initiation factor 2B complex	2	1
GO:0032991	protein-containing complex	1	1
GO:0033290	eukaryotic 48S preinitiation complex	4	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0043614	multi-eIF complex	3	1
GO:0070993	translation preinitiation complex	3	1
GO:0110165	cellular anatomical entity	1	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

A4H3R0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H3R0

Function

Biological processes
Term	Name	Level	Count
GO:0006413	translational initiation	3	1
GO:0006950	response to stress	2	1
GO:0007154	cell communication	2	1
GO:0008152	metabolic process	1	1
GO:0009267	cellular response to starvation	4	1
GO:0009605	response to external stimulus	2	1
GO:0009987	cellular process	1	1
GO:0009991	response to extracellular stimulus	3	1
GO:0010468	regulation of gene expression	5	1
GO:0010608	post-transcriptional regulation of gene expression	6	1
GO:0019222	regulation of metabolic process	3	1
GO:0031667	response to nutrient levels	4	1
GO:0031668	cellular response to extracellular stimulus	3	1
GO:0031669	cellular response to nutrient levels	4	1
GO:0033554	cellular response to stress	3	1
GO:0042594	response to starvation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0050789	regulation of biological process	2	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0071496	cellular response to external stimulus	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003743	translation initiation factor activity	4	12
GO:0005488	binding	1	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0043021	ribonucleoprotein complex binding	3	1
GO:0043022	ribosome binding	4	1
GO:0044877	protein-containing complex binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	367	371	PF00656	0.314
CLV_C14_Caspase3-7	5	9	PF00656	0.422
CLV_NRD_NRD_1	168	170	PF00675	0.354
CLV_NRD_NRD_1	44	46	PF00675	0.506
CLV_PCSK_KEX2_1	100	102	PF00082	0.447
CLV_PCSK_KEX2_1	155	157	PF00082	0.354
CLV_PCSK_KEX2_1	167	169	PF00082	0.354
CLV_PCSK_KEX2_1	378	380	PF00082	0.483
CLV_PCSK_KEX2_1	44	46	PF00082	0.506
CLV_PCSK_PC1ET2_1	100	102	PF00082	0.447
CLV_PCSK_PC1ET2_1	155	157	PF00082	0.354
CLV_PCSK_PC1ET2_1	378	380	PF00082	0.470
CLV_PCSK_PC7_1	96	102	PF00082	0.538
CLV_PCSK_SKI1_1	175	179	PF00082	0.250
CLV_PCSK_SKI1_1	202	206	PF00082	0.390
CLV_PCSK_SKI1_1	298	302	PF00082	0.339
CLV_PCSK_SKI1_1	320	324	PF00082	0.261
CLV_PCSK_SKI1_1	384	388	PF00082	0.652
CLV_PCSK_SKI1_1	45	49	PF00082	0.496
DOC_ANK_TNKS_1	326	333	PF00023	0.554
DOC_CKS1_1	203	208	PF01111	0.483
DOC_CYCLIN_RxL_1	292	305	PF00134	0.534
DOC_CYCLIN_RxL_1	42	51	PF00134	0.597
DOC_CYCLIN_yCln2_LP_2	49	55	PF00134	0.519
DOC_MAPK_gen_1	377	385	PF00069	0.441
DOC_MAPK_HePTP_8	57	69	PF00069	0.518
DOC_MAPK_MEF2A_6	129	138	PF00069	0.461
DOC_MAPK_MEF2A_6	292	299	PF00069	0.564
DOC_MAPK_MEF2A_6	60	69	PF00069	0.519
DOC_MAPK_NFAT4_5	292	300	PF00069	0.498
DOC_PP4_FxxP_1	53	56	PF00568	0.409
DOC_USP7_MATH_1	56	60	PF00917	0.335
DOC_USP7_UBL2_3	100	104	PF12436	0.469
DOC_WW_Pin1_4	202	207	PF00397	0.483
DOC_WW_Pin1_4	6	11	PF00397	0.539
LIG_14-3-3_CanoR_1	168	174	PF00244	0.554
LIG_14-3-3_CanoR_1	37	43	PF00244	0.496
LIG_Actin_WH2_2	276	294	PF00022	0.527
LIG_Actin_WH2_2	369	386	PF00022	0.420
LIG_APCC_ABBA_1	159	164	PF00400	0.450
LIG_APCC_ABBA_1	300	305	PF00400	0.554
LIG_BIR_II_1	1	5	PF00653	0.496
LIG_Clathr_ClatBox_1	66	70	PF01394	0.399
LIG_DLG_GKlike_1	169	176	PF00625	0.554
LIG_FAT_LD_1	62	70	PF03623	0.434
LIG_FHA_1	142	148	PF00498	0.452
LIG_FHA_1	22	28	PF00498	0.442
LIG_FHA_1	265	271	PF00498	0.465
LIG_FHA_1	371	377	PF00498	0.461
LIG_FHA_2	111	117	PF00498	0.474
LIG_FHA_2	203	209	PF00498	0.386
LIG_HCF-1_HBM_1	25	28	PF13415	0.315
LIG_LIR_Apic_2	50	56	PF02991	0.451
LIG_LIR_Gen_1	132	141	PF02991	0.450
LIG_LIR_Gen_1	193	204	PF02991	0.450
LIG_LIR_Gen_1	301	311	PF02991	0.554
LIG_LIR_Nem_3	132	138	PF02991	0.450
LIG_LIR_Nem_3	193	199	PF02991	0.450
LIG_LIR_Nem_3	25	31	PF02991	0.431
LIG_LIR_Nem_3	301	306	PF02991	0.554
LIG_LIR_Nem_3	34	38	PF02991	0.481
LIG_NRBOX	61	67	PF00104	0.477
LIG_Pex14_1	258	262	PF04695	0.517
LIG_RPA_C_Fungi	164	176	PF08784	0.430
LIG_SH2_CRK	29	33	PF00017	0.414
LIG_SH2_NCK_1	162	166	PF00017	0.300
LIG_SH2_PTP2	196	199	PF00017	0.285
LIG_SH2_STAT5	123	126	PF00017	0.511
LIG_SH2_STAT5	162	165	PF00017	0.285
LIG_SH2_STAT5	196	199	PF00017	0.285
LIG_SH2_STAT5	35	38	PF00017	0.446
LIG_SH3_3	383	389	PF00018	0.456
LIG_SUMO_SIM_anti_2	321	326	PF11976	0.319
LIG_SUMO_SIM_anti_2	63	70	PF11976	0.465
LIG_SUMO_SIM_par_1	63	70	PF11976	0.391
LIG_TYR_ITIM	160	165	PF00017	0.300
LIG_UBA3_1	273	281	PF00899	0.319
LIG_UBA3_1	287	292	PF00899	0.319
LIG_UBA3_1	65	71	PF00899	0.505
LIG_WW_1	346	349	PF00397	0.285
MOD_CK1_1	9	15	PF00069	0.569
MOD_CK2_1	202	208	PF00069	0.389
MOD_CK2_1	84	90	PF00069	0.672
MOD_Cter_Amidation	153	156	PF01082	0.430
MOD_Cter_Amidation	397	400	PF01082	0.670
MOD_GlcNHglycan	143	147	PF01048	0.285
MOD_GlcNHglycan	75	78	PF01048	0.501
MOD_GSK3_1	2	9	PF00069	0.523
MOD_GSK3_1	47	54	PF00069	0.533
MOD_N-GLC_1	141	146	PF02516	0.286
MOD_N-GLC_1	264	269	PF02516	0.319
MOD_N-GLC_1	38	43	PF02516	0.548
MOD_NEK2_1	110	115	PF00069	0.480
MOD_NEK2_1	287	292	PF00069	0.337
MOD_NEK2_1	38	43	PF00069	0.586
MOD_PIKK_1	136	142	PF00454	0.430
MOD_PKB_1	167	175	PF00069	0.379
MOD_Plk_1	142	148	PF00069	0.300
MOD_Plk_1	306	312	PF00069	0.344
MOD_Plk_1	38	44	PF00069	0.594
MOD_Plk_2-3	58	64	PF00069	0.480
MOD_Plk_4	298	304	PF00069	0.488
MOD_Plk_4	70	76	PF00069	0.454
MOD_ProDKin_1	202	208	PF00069	0.483
MOD_ProDKin_1	6	12	PF00069	0.541
MOD_SUMO_for_1	357	360	PF00179	0.494
MOD_SUMO_rev_2	114	120	PF00179	0.575
MOD_SUMO_rev_2	54	62	PF00179	0.453
MOD_SUMO_rev_2	8	17	PF00179	0.500
MOD_SUMO_rev_2	81	87	PF00179	0.654
TRG_ENDOCYTIC_2	162	165	PF00928	0.285
TRG_ENDOCYTIC_2	196	199	PF00928	0.285
TRG_ENDOCYTIC_2	28	31	PF00928	0.422
TRG_ENDOCYTIC_2	35	38	PF00928	0.463
TRG_ER_diArg_1	167	169	PF00400	0.285
TRG_ER_diArg_1	351	354	PF00400	0.316
TRG_NES_CRM1_1	125	140	PF08389	0.430

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P537	Leptomonas seymouri	86%	100%
A0A0S4IW47	Bodo saltans	46%	100%
A0A1X0P943	Trypanosomatidae	62%	99%
A0A3S7WNN3	Leishmania donovani	95%	100%
A0A422NET9	Trypanosoma rangeli	59%	99%
A4HRY4	Leishmania infantum	95%	100%
C9ZKJ3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	99%
E9ACP3	Leishmania major	95%	100%
E9AJY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
P05198	Homo sapiens	31%	100%
P20459	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	33%	100%
P41374	Drosophila melanogaster	33%	100%
P56286	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
P68101	Rattus norvegicus	31%	100%
P68102	Bos taurus	31%	100%
Q5R493	Pongo abelii	31%	100%
Q5ZLX2	Gallus gallus	31%	100%
Q6GL89	Xenopus tropicalis	32%	100%
Q6ZWX6	Mus musculus	31%	100%
Q7ZTK4	Xenopus laevis	32%	100%
Q869N9	Dictyostelium discoideum	31%	100%
Q9FE78	Arabidopsis thaliana	31%	100%
Q9SIZ2	Arabidopsis thaliana	34%	100%
V5BSN3	Trypanosoma cruzi	60%	99%