tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Met-10+ like-protein, putative

Species:

Leishmania braziliensis

UniProt:

A4H3H8_LEIBR

TriTrypDb:

LbrM.03.0070 , LBRM2903_030005600

Length:

390

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	2
GO:0110165	cellular anatomical entity	1	3
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

A4H3H8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H3H8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006400	tRNA modification	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0001510	RNA methylation	4	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	3
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0016740	transferase activity	2	12
GO:0016765	transferase activity, transferring alkyl or aryl (other than methyl) groups	3	12
GO:0102522	tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0008168	methyltransferase activity	4	3
GO:0008173	RNA methyltransferase activity	4	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0016741	transferase activity, transferring one-carbon groups	3	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	274	280	PF00089	0.230
CLV_NRD_NRD_1	134	136	PF00675	0.356
CLV_NRD_NRD_1	156	158	PF00675	0.410
CLV_NRD_NRD_1	161	163	PF00675	0.360
CLV_NRD_NRD_1	44	46	PF00675	0.497
CLV_NRD_NRD_1	76	78	PF00675	0.327
CLV_NRD_NRD_1	80	82	PF00675	0.318
CLV_PCSK_FUR_1	35	39	PF00082	0.556
CLV_PCSK_KEX2_1	134	136	PF00082	0.380
CLV_PCSK_KEX2_1	156	158	PF00082	0.399
CLV_PCSK_KEX2_1	161	163	PF00082	0.315
CLV_PCSK_KEX2_1	168	170	PF00082	0.213
CLV_PCSK_KEX2_1	185	187	PF00082	0.295
CLV_PCSK_KEX2_1	323	325	PF00082	0.293
CLV_PCSK_KEX2_1	34	36	PF00082	0.497
CLV_PCSK_KEX2_1	37	39	PF00082	0.534
CLV_PCSK_KEX2_1	379	381	PF00082	0.341
CLV_PCSK_KEX2_1	76	78	PF00082	0.309
CLV_PCSK_PC1ET2_1	168	170	PF00082	0.409
CLV_PCSK_PC1ET2_1	185	187	PF00082	0.295
CLV_PCSK_PC1ET2_1	323	325	PF00082	0.338
CLV_PCSK_PC1ET2_1	34	36	PF00082	0.503
CLV_PCSK_PC1ET2_1	37	39	PF00082	0.535
CLV_PCSK_PC1ET2_1	379	381	PF00082	0.341
CLV_PCSK_PC7_1	157	163	PF00082	0.399
CLV_PCSK_SKI1_1	209	213	PF00082	0.263
CLV_PCSK_SKI1_1	312	316	PF00082	0.338
CLV_PCSK_SKI1_1	34	38	PF00082	0.564
CLV_PCSK_SKI1_1	371	375	PF00082	0.293
CLV_PCSK_SKI1_1	46	50	PF00082	0.548
CLV_PCSK_SKI1_1	76	80	PF00082	0.400
DEG_APCC_DBOX_1	134	142	PF00400	0.580
DOC_MAPK_gen_1	134	142	PF00069	0.599
DOC_MAPK_MEF2A_6	134	142	PF00069	0.599
DOC_PP1_RVXF_1	207	213	PF00149	0.447
DOC_PP1_RVXF_1	218	224	PF00149	0.446
DOC_PP2B_LxvP_1	26	29	PF13499	0.527
DOC_PP4_FxxP_1	243	246	PF00568	0.452
DOC_USP7_MATH_1	179	183	PF00917	0.610
DOC_USP7_MATH_1	68	72	PF00917	0.594
DOC_WW_Pin1_4	187	192	PF00397	0.546
DOC_WW_Pin1_4	49	54	PF00397	0.665
LIG_14-3-3_CanoR_1	186	193	PF00244	0.585
LIG_14-3-3_CanoR_1	317	322	PF00244	0.452
LIG_14-3-3_CanoR_1	366	373	PF00244	0.516
LIG_Actin_WH2_2	336	353	PF00022	0.607
LIG_Actin_WH2_2	358	373	PF00022	0.571
LIG_BIR_II_1	1	5	PF00653	0.386
LIG_BRCT_BRCA1_1	189	193	PF00533	0.531
LIG_BRCT_BRCA1_1	264	268	PF00533	0.477
LIG_BRCT_BRCA1_2	264	270	PF00533	0.477
LIG_Clathr_ClatBox_1	123	127	PF01394	0.580
LIG_CtBP_PxDLS_1	293	299	PF00389	0.377
LIG_DLG_GKlike_1	134	141	PF00625	0.599
LIG_FHA_1	192	198	PF00498	0.460
LIG_FHA_1	216	222	PF00498	0.518
LIG_FHA_1	58	64	PF00498	0.549
LIG_FHA_2	96	102	PF00498	0.574
LIG_GBD_Chelix_1	15	23	PF00786	0.305
LIG_LIR_Gen_1	265	275	PF02991	0.493
LIG_LIR_Nem_3	113	117	PF02991	0.550
LIG_LIR_Nem_3	235	240	PF02991	0.444
LIG_LIR_Nem_3	265	271	PF02991	0.452
LIG_LYPXL_yS_3	67	70	PF13949	0.440
LIG_Pex14_2	110	114	PF04695	0.499
LIG_Pex14_2	79	83	PF04695	0.508
LIG_SH2_CRK	336	340	PF00017	0.474
LIG_SH2_STAP1	151	155	PF00017	0.591
LIG_SH2_STAP1	203	207	PF00017	0.481
LIG_SH2_STAT5	20	23	PF00017	0.352
LIG_SH2_STAT5	242	245	PF00017	0.452
LIG_SH2_STAT5	307	310	PF00017	0.448
LIG_SUMO_SIM_anti_2	118	123	PF11976	0.460
LIG_SUMO_SIM_anti_2	13	19	PF11976	0.223
LIG_SUMO_SIM_anti_2	2	7	PF11976	0.357
LIG_SUMO_SIM_par_1	115	120	PF11976	0.531
LIG_SUMO_SIM_par_1	122	127	PF11976	0.488
LIG_SUMO_SIM_par_1	7	13	PF11976	0.338
LIG_TRAF2_1	345	348	PF00917	0.554
LIG_TRAF2_1	99	102	PF00917	0.558
LIG_TYR_ITIM	18	23	PF00017	0.315
LIG_TYR_ITIM	240	245	PF00017	0.305
LIG_TYR_ITIM	334	339	PF00017	0.336
LIG_UBA3_1	304	312	PF00899	0.420
LIG_WRC_WIRS_1	202	207	PF05994	0.425
MOD_CDK_SPxK_1	49	55	PF00069	0.372
MOD_CK1_1	10	16	PF00069	0.418
MOD_CK1_1	214	220	PF00069	0.374
MOD_CK1_1	71	77	PF00069	0.420
MOD_CK2_1	95	101	PF00069	0.507
MOD_GlcNHglycan	213	216	PF01048	0.305
MOD_GlcNHglycan	228	231	PF01048	0.305
MOD_GlcNHglycan	330	333	PF01048	0.438
MOD_GSK3_1	146	153	PF00069	0.480
MOD_GSK3_1	185	192	PF00069	0.359
MOD_GSK3_1	201	208	PF00069	0.285
MOD_GSK3_1	210	217	PF00069	0.121
MOD_GSK3_1	361	368	PF00069	0.267
MOD_N-GLC_1	215	220	PF02516	0.305
MOD_NEK2_1	110	115	PF00069	0.363
MOD_NEK2_1	155	160	PF00069	0.464
MOD_NEK2_1	180	185	PF00069	0.448
MOD_NEK2_1	205	210	PF00069	0.372
MOD_NEK2_1	211	216	PF00069	0.181
MOD_NEK2_1	59	64	PF00069	0.438
MOD_NEK2_2	151	156	PF00069	0.438
MOD_PK_1	7	13	PF00069	0.199
MOD_PKA_1	134	140	PF00069	0.488
MOD_PKA_1	161	167	PF00069	0.410
MOD_PKA_1	185	191	PF00069	0.528
MOD_PKA_2	134	140	PF00069	0.484
MOD_PKA_2	155	161	PF00069	0.472
MOD_PKA_2	185	191	PF00069	0.529
MOD_PKA_2	328	334	PF00069	0.440
MOD_PKA_2	365	371	PF00069	0.504
MOD_Plk_1	180	186	PF00069	0.528
MOD_Plk_1	205	211	PF00069	0.408
MOD_Plk_1	68	74	PF00069	0.504
MOD_Plk_2-3	96	102	PF00069	0.508
MOD_Plk_4	10	16	PF00069	0.379
MOD_Plk_4	310	316	PF00069	0.348
MOD_Plk_4	317	323	PF00069	0.285
MOD_ProDKin_1	187	193	PF00069	0.429
MOD_ProDKin_1	49	55	PF00069	0.587
MOD_SUMO_rev_2	40	48	PF00179	0.687
TRG_ENDOCYTIC_2	20	23	PF00928	0.315
TRG_ENDOCYTIC_2	242	245	PF00928	0.305
TRG_ENDOCYTIC_2	336	339	PF00928	0.332
TRG_ENDOCYTIC_2	67	70	PF00928	0.287
TRG_ER_diArg_1	133	135	PF00400	0.490
TRG_ER_diArg_1	155	157	PF00400	0.522
TRG_ER_diArg_1	161	163	PF00400	0.511
TRG_ER_diArg_1	75	77	PF00400	0.383
TRG_NES_CRM1_1	355	369	PF08389	0.464
TRG_NLS_MonoExtC_3	33	38	PF00514	0.715

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4L5	Leptomonas seymouri	64%	97%
A0A0S4JHI6	Bodo saltans	49%	95%
A0A1X0P9M7	Trypanosomatidae	53%	95%
A0A422N7F4	Trypanosoma rangeli	52%	100%
A0A451EJL2	Leishmania donovani	83%	100%
D0A212	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	99%
E9ACF3	Leishmania major	84%	100%
E9AG25	Leishmania infantum	83%	100%
E9AJP1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
Q93YU6	Arabidopsis thaliana	24%	83%
V5B8P6	Trypanosoma cruzi	51%	100%