Putative ubiquitin-conjugating enzyme e2

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania braziliensis

UniProt:

A4H3D5_LEIBR

TriTrypDb:

LbrM.02.0420 , LBRM2903_020009600

Length:

223

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 14
Forrest at al. (procyclic)	no	yes: 14
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 30

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 42

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 19
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	3
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0110165	cellular anatomical entity	1	3
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

A4H3D5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H3D5

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	2
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	3
GO:0032446	protein modification by small protein conjugation	6	3
GO:0036211	protein modification process	4	3
GO:0043170	macromolecule metabolic process	3	3
GO:0043412	macromolecule modification	4	3
GO:0044238	primary metabolic process	2	3
GO:0070647	protein modification by small protein conjugation or removal	5	3
GO:0071704	organic substance metabolic process	2	3
GO:1901564	organonitrogen compound metabolic process	3	3
GO:0016925	protein sumoylation	7	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018205	peptidyl-lysine modification	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006259	DNA metabolic process	4	1
GO:0006281	DNA repair	5	1
GO:0006301	postreplication repair	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006950	response to stress	2	1
GO:0006974	DNA damage response	4	1
GO:0009987	cellular process	1	1
GO:0033554	cellular response to stress	3	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0070534	protein K63-linked ubiquitination	9	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	20
GO:0003824	catalytic activity	1	20
GO:0005488	binding	1	20
GO:0005524	ATP binding	5	20
GO:0016740	transferase activity	2	18
GO:0017076	purine nucleotide binding	4	20
GO:0019787	ubiquitin-like protein transferase activity	3	11
GO:0019789	SUMO transferase activity	4	9
GO:0030554	adenyl nucleotide binding	5	20
GO:0032553	ribonucleotide binding	3	20
GO:0032555	purine ribonucleotide binding	4	20
GO:0032559	adenyl ribonucleotide binding	5	20
GO:0035639	purine ribonucleoside triphosphate binding	4	20
GO:0036094	small molecule binding	2	20
GO:0043167	ion binding	2	20
GO:0043168	anion binding	3	20
GO:0097159	organic cyclic compound binding	2	20
GO:0097367	carbohydrate derivative binding	2	20
GO:0140096	catalytic activity, acting on a protein	2	11
GO:1901265	nucleoside phosphate binding	3	20
GO:1901363	heterocyclic compound binding	2	20
GO:0004842	ubiquitin-protein transferase activity	4	3
GO:0016746	acyltransferase activity	3	1
GO:0016874	ligase activity	2	9
GO:0061631	ubiquitin conjugating enzyme activity	5	3
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	4
GO:0061656	SUMO conjugating enzyme activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	14	16	PF00675	0.408
CLV_NRD_NRD_1	19	21	PF00675	0.426
CLV_NRD_NRD_1	216	218	PF00675	0.545
CLV_PCSK_KEX2_1	19	21	PF00082	0.425
CLV_PCSK_PC7_1	15	21	PF00082	0.406
CLV_PCSK_SKI1_1	138	142	PF00082	0.264
CLV_PCSK_SKI1_1	174	178	PF00082	0.306
CLV_PCSK_SKI1_1	203	207	PF00082	0.366
DEG_SPOP_SBC_1	66	70	PF00917	0.621
DOC_CKS1_1	113	118	PF01111	0.253
DOC_CKS1_1	140	145	PF01111	0.341
DOC_MAPK_gen_1	165	173	PF00069	0.236
DOC_MAPK_gen_1	44	54	PF00069	0.485
DOC_MAPK_MEF2A_6	165	173	PF00069	0.279
DOC_PP1_RVXF_1	123	129	PF00149	0.294
DOC_PP2B_LxvP_1	52	55	PF13499	0.471
DOC_PP2B_PxIxI_1	168	174	PF00149	0.220
DOC_USP7_MATH_1	55	59	PF00917	0.503
DOC_USP7_MATH_1	66	70	PF00917	0.489
DOC_USP7_UBL2_3	218	222	PF12436	0.466
DOC_WW_Pin1_4	112	117	PF00397	0.281
DOC_WW_Pin1_4	139	144	PF00397	0.341
DOC_WW_Pin1_4	93	98	PF00397	0.631
LIG_14-3-3_CanoR_1	123	129	PF00244	0.315
LIG_FHA_1	140	146	PF00498	0.331
LIG_FHA_1	153	159	PF00498	0.208
LIG_FHA_2	113	119	PF00498	0.253
LIG_LIR_Nem_3	195	201	PF02991	0.220
LIG_SH2_STAP1	208	212	PF00017	0.220
LIG_SH3_2	133	138	PF14604	0.294
LIG_SH3_3	107	113	PF00018	0.249
LIG_SH3_3	130	136	PF00018	0.299
LIG_SH3_3	137	143	PF00018	0.294
LIG_SUMO_SIM_par_1	176	182	PF11976	0.262
LIG_TRAF2_1	179	182	PF00917	0.220
LIG_TRAF2_1	192	195	PF00917	0.220
MOD_CK1_1	68	74	PF00069	0.658
MOD_CK1_1	75	81	PF00069	0.770
MOD_CK1_1	88	94	PF00069	0.539
MOD_CK2_1	112	118	PF00069	0.287
MOD_CK2_1	124	130	PF00069	0.177
MOD_CK2_1	81	87	PF00069	0.549
MOD_GlcNHglycan	152	155	PF01048	0.229
MOD_GlcNHglycan	40	43	PF01048	0.549
MOD_GlcNHglycan	57	60	PF01048	0.471
MOD_GlcNHglycan	77	80	PF01048	0.648
MOD_GlcNHglycan	90	93	PF01048	0.556
MOD_GSK3_1	152	159	PF00069	0.253
MOD_GSK3_1	2	9	PF00069	0.402
MOD_GSK3_1	34	41	PF00069	0.523
MOD_GSK3_1	53	60	PF00069	0.559
MOD_GSK3_1	62	69	PF00069	0.489
MOD_GSK3_1	70	77	PF00069	0.502
MOD_GSK3_1	78	85	PF00069	0.545
MOD_LATS_1	45	51	PF00433	0.478
MOD_N-GLC_1	169	174	PF02516	0.297
MOD_NEK2_1	124	129	PF00069	0.177
MOD_NEK2_1	6	11	PF00069	0.398
MOD_NEK2_2	169	174	PF00069	0.231
MOD_PIKK_1	47	53	PF00454	0.501
MOD_PKA_2	124	130	PF00069	0.177
MOD_PKA_2	38	44	PF00069	0.484
MOD_Plk_1	169	175	PF00069	0.294
MOD_Plk_4	156	162	PF00069	0.221
MOD_ProDKin_1	112	118	PF00069	0.281
MOD_ProDKin_1	139	145	PF00069	0.341
MOD_ProDKin_1	93	99	PF00069	0.348
MOD_SUMO_rev_2	9	17	PF00179	0.387
TRG_ER_diArg_1	122	125	PF00400	0.294
TRG_ER_diArg_1	18	20	PF00400	0.473

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PAW7	Leptomonas seymouri	74%	100%
A0A0S4IJE0	Bodo saltans	34%	100%
A0A1X0NT81	Trypanosomatidae	36%	100%
A0A1X0NV24	Trypanosomatidae	32%	90%
A0A3Q8II46	Leishmania donovani	39%	100%
A0A3R7L4E4	Trypanosoma rangeli	69%	98%
A0A3R7LRA8	Trypanosoma rangeli	31%	69%
A0A3S5H5C0	Leishmania donovani	36%	100%
A0A3S7WYH9	Leishmania donovani	32%	100%
A0A3S7WYK3	Leishmania donovani	36%	100%
A0A451EJH5	Leishmania donovani	88%	100%
A3KN22	Bos taurus	28%	100%
A4H402	Leishmania braziliensis	41%	100%
A4HDN1	Leishmania braziliensis	31%	100%
A4HDS4	Leishmania braziliensis	36%	100%
A4HMF2	Leishmania braziliensis	39%	100%
A4HRN6	Leishmania infantum	88%	100%
A4HS64	Leishmania infantum	36%	100%
A4I122	Leishmania infantum	36%	100%
A4IB24	Leishmania infantum	39%	100%
B4L7V4	Drosophila mojavensis	32%	100%
C9ZJ18	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	100%
E9ACB4	Leishmania major	94%	100%
E9AH78	Leishmania infantum	32%	100%
E9AJK2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
E9AK51	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9AX16	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9AX59	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
E9B609	Leishmania mexicana (strain MHOM/GT/2001/U1103)	39%	100%
P52483	Mus musculus	30%	100%
P56616	Xenopus laevis	28%	100%
P61081	Homo sapiens	28%	100%
P61082	Mus musculus	28%	100%
Q02159	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
Q2T9X7	Bos taurus	30%	100%
Q4QAB7	Leishmania major	36%	100%
Q4QAG0	Leishmania major	31%	100%
Q66KB0	Xenopus tropicalis	28%	71%
Q6DCZ9	Xenopus laevis	31%	100%
Q6NY82	Danio rerio	24%	100%
Q6P8D9	Xenopus tropicalis	31%	100%
Q75AF2	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	28%	100%
Q969T4	Homo sapiens	30%	100%
Q9GNC1	Leishmania major	39%	100%
Q9LJD7	Arabidopsis thaliana	36%	100%
Q9NED4	Leishmania major	36%	100%
Q9ZU75	Arabidopsis thaliana	29%	100%