Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

FtsJ-like methyltransferase, putative

Species:

Leishmania braziliensis

UniProt:

A4H3D4_LEIBR

TriTrypDb:

LbrM.02.0410 , LBRM2903_020009500

Length:

457

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	10
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

A4H3D4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H3D4

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	10
GO:0002128	tRNA nucleoside ribose methylation	6	10
GO:0002181	cytoplasmic translation	5	10
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006396	RNA processing	6	10
GO:0006399	tRNA metabolic process	7	10
GO:0006400	tRNA modification	6	10
GO:0006412	translation	4	10
GO:0006518	peptide metabolic process	4	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008033	tRNA processing	8	10
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	10
GO:0009059	macromolecule biosynthetic process	4	10
GO:0009451	RNA modification	5	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0019538	protein metabolic process	3	10
GO:0030488	tRNA methylation	5	10
GO:0032259	methylation	2	12
GO:0034470	ncRNA processing	7	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0034645	obsolete cellular macromolecule biosynthetic process	4	10
GO:0034660	ncRNA metabolic process	6	10
GO:0043043	peptide biosynthetic process	5	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0043414	macromolecule methylation	3	10
GO:0043603	amide metabolic process	3	10
GO:0043604	amide biosynthetic process	4	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0044249	cellular biosynthetic process	3	10
GO:0044260	obsolete cellular macromolecule metabolic process	3	10
GO:0044271	cellular nitrogen compound biosynthetic process	4	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10
GO:1901564	organonitrogen compound metabolic process	3	10
GO:1901566	organonitrogen compound biosynthetic process	4	10
GO:1901576	organic substance biosynthetic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	10
GO:0008175	tRNA methyltransferase activity	5	10
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0140098	catalytic activity, acting on RNA	3	10
GO:0140101	catalytic activity, acting on a tRNA	4	10
GO:0140640	catalytic activity, acting on a nucleic acid	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	13	15	PF00675	0.417
CLV_NRD_NRD_1	163	165	PF00675	0.312
CLV_NRD_NRD_1	304	306	PF00675	0.545
CLV_PCSK_KEX2_1	304	306	PF00082	0.545
CLV_PCSK_KEX2_1	8	10	PF00082	0.465
CLV_PCSK_PC1ET2_1	8	10	PF00082	0.465
CLV_PCSK_SKI1_1	14	18	PF00082	0.395
CLV_PCSK_SKI1_1	234	238	PF00082	0.316
DEG_SPOP_SBC_1	148	152	PF00917	0.511
DOC_MAPK_gen_1	164	170	PF00069	0.487
DOC_PP2B_LxvP_1	124	127	PF13499	0.549
DOC_PP2B_LxvP_1	401	404	PF13499	0.577
DOC_PP4_FxxP_1	214	217	PF00568	0.469
DOC_PP4_FxxP_1	255	258	PF00568	0.399
DOC_USP7_MATH_1	143	147	PF00917	0.566
DOC_USP7_MATH_1	148	152	PF00917	0.523
DOC_USP7_MATH_1	238	242	PF00917	0.469
DOC_USP7_MATH_1	282	286	PF00917	0.693
DOC_USP7_MATH_1	308	312	PF00917	0.667
DOC_USP7_MATH_1	333	337	PF00917	0.532
DOC_USP7_MATH_1	339	343	PF00917	0.550
DOC_USP7_MATH_1	352	356	PF00917	0.433
DOC_USP7_MATH_1	409	413	PF00917	0.533
DOC_USP7_MATH_1	449	453	PF00917	0.534
DOC_WW_Pin1_4	318	323	PF00397	0.698
DOC_WW_Pin1_4	84	89	PF00397	0.574
LIG_14-3-3_CanoR_1	215	223	PF00244	0.512
LIG_14-3-3_CanoR_1	22	30	PF00244	0.466
LIG_14-3-3_CanoR_1	242	247	PF00244	0.469
LIG_14-3-3_CanoR_1	307	311	PF00244	0.517
LIG_14-3-3_CanoR_1	357	363	PF00244	0.655
LIG_Actin_WH2_2	189	206	PF00022	0.478
LIG_APCC_ABBAyCdc20_2	225	231	PF00400	0.465
LIG_BRCT_BRCA1_1	225	229	PF00533	0.487
LIG_Clathr_ClatBox_1	29	33	PF01394	0.465
LIG_eIF4E_1	186	192	PF01652	0.469
LIG_FHA_1	110	116	PF00498	0.554
LIG_FHA_1	197	203	PF00498	0.465
LIG_FHA_1	217	223	PF00498	0.455
LIG_FHA_1	44	50	PF00498	0.483
LIG_FHA_1	85	91	PF00498	0.510
LIG_FHA_2	114	120	PF00498	0.469
LIG_FHA_2	133	139	PF00498	0.469
LIG_FHA_2	359	365	PF00498	0.692
LIG_LIR_Apic_2	211	217	PF02991	0.469
LIG_LIR_Gen_1	227	238	PF02991	0.490
LIG_LIR_Gen_1	33	44	PF02991	0.491
LIG_LIR_Gen_1	57	66	PF02991	0.469
LIG_LIR_Nem_3	227	233	PF02991	0.414
LIG_LIR_Nem_3	25	30	PF02991	0.469
LIG_LIR_Nem_3	33	39	PF02991	0.469
LIG_LIR_Nem_3	57	61	PF02991	0.469
LIG_PCNA_PIPBox_1	180	189	PF02747	0.487
LIG_PCNA_yPIPBox_3	237	250	PF02747	0.469
LIG_SH2_CRK	27	31	PF00017	0.469
LIG_SH2_STAT5	186	189	PF00017	0.469
LIG_SH2_STAT5	261	264	PF00017	0.533
LIG_SH2_STAT5	327	330	PF00017	0.688
LIG_SH2_STAT5	405	408	PF00017	0.542
LIG_SH3_3	232	238	PF00018	0.536
LIG_SH3_3	319	325	PF00018	0.592
LIG_SH3_3	363	369	PF00018	0.517
LIG_SH3_3	390	396	PF00018	0.544
LIG_SUMO_SIM_anti_2	102	108	PF11976	0.510
LIG_SUMO_SIM_par_1	89	94	PF11976	0.469
LIG_TRAF2_1	16	19	PF00917	0.392
LIG_TRAF2_1	451	454	PF00917	0.539
LIG_WRC_WIRS_1	198	203	PF05994	0.469
LIG_WRC_WIRS_1	246	251	PF05994	0.549
LIG_WW_1	258	261	PF00397	0.304
MOD_CDK_SPK_2	84	89	PF00069	0.510
MOD_CK1_1	113	119	PF00069	0.487
MOD_CK1_1	146	152	PF00069	0.514
MOD_CK1_1	245	251	PF00069	0.549
MOD_CK1_1	272	278	PF00069	0.634
MOD_CK1_1	283	289	PF00069	0.674
MOD_CK1_1	318	324	PF00069	0.637
MOD_CK1_1	355	361	PF00069	0.525
MOD_CK2_1	113	119	PF00069	0.469
MOD_CK2_1	132	138	PF00069	0.469
MOD_CK2_1	287	293	PF00069	0.681
MOD_CK2_1	358	364	PF00069	0.442
MOD_Cter_Amidation	302	305	PF01082	0.546
MOD_GlcNHglycan	24	27	PF01048	0.269
MOD_GlcNHglycan	266	269	PF01048	0.599
MOD_GlcNHglycan	271	274	PF01048	0.642
MOD_GlcNHglycan	277	280	PF01048	0.644
MOD_GlcNHglycan	282	285	PF01048	0.645
MOD_GlcNHglycan	310	313	PF01048	0.777
MOD_GlcNHglycan	317	320	PF01048	0.713
MOD_GlcNHglycan	341	344	PF01048	0.571
MOD_GlcNHglycan	375	379	PF01048	0.522
MOD_GlcNHglycan	415	418	PF01048	0.528
MOD_GSK3_1	109	116	PF00069	0.469
MOD_GSK3_1	142	149	PF00069	0.502
MOD_GSK3_1	238	245	PF00069	0.469
MOD_GSK3_1	269	276	PF00069	0.583
MOD_GSK3_1	278	285	PF00069	0.603
MOD_GSK3_1	328	335	PF00069	0.674
MOD_GSK3_1	352	359	PF00069	0.672
MOD_GSK3_1	409	416	PF00069	0.454
MOD_N-GLC_1	127	132	PF02516	0.203
MOD_N-GLC_1	242	247	PF02516	0.269
MOD_N-GLC_1	449	454	PF02516	0.536
MOD_N-GLC_1	71	76	PF02516	0.292
MOD_NEK2_1	159	164	PF00069	0.431
MOD_NEK2_1	196	201	PF00069	0.469
MOD_NEK2_1	315	320	PF00069	0.655
MOD_PIKK_1	159	165	PF00454	0.397
MOD_PIKK_1	352	358	PF00454	0.618
MOD_PIKK_1	437	443	PF00454	0.592
MOD_PIKK_1	56	62	PF00454	0.469
MOD_PKA_2	2	8	PF00069	0.530
MOD_PKA_2	306	312	PF00069	0.603
MOD_PKA_2	315	321	PF00069	0.631
MOD_PKA_2	356	362	PF00069	0.691
MOD_Plk_1	242	248	PF00069	0.469
MOD_Plk_4	197	203	PF00069	0.466
MOD_Plk_4	245	251	PF00069	0.540
MOD_ProDKin_1	318	324	PF00069	0.693
MOD_ProDKin_1	84	90	PF00069	0.574
MOD_SUMO_for_1	16	19	PF00179	0.392
MOD_SUMO_for_1	224	227	PF00179	0.414
TRG_DiLeu_BaEn_1	119	124	PF01217	0.507
TRG_DiLeu_BaEn_3	33	39	PF01217	0.397
TRG_DiLeu_BaEn_4	34	40	PF01217	0.487
TRG_DiLeu_BaEn_4	389	395	PF01217	0.465
TRG_DiLeu_BaLyEn_6	261	266	PF01217	0.333
TRG_DiLeu_LyEn_5	119	124	PF01217	0.487
TRG_ENDOCYTIC_2	27	30	PF00928	0.455
TRG_ER_diArg_1	21	24	PF00400	0.466
TRG_NES_CRM1_1	96	110	PF08389	0.469
TRG_NLS_MonoExtC_3	433	439	PF00514	0.660
TRG_NLS_MonoExtN_4	431	438	PF00514	0.703

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HTF7	Leptomonas seymouri	60%	100%
A0A0S4JD71	Bodo saltans	47%	100%
A0A3S7X469	Leishmania donovani	24%	100%
A0A451EJH4	Leishmania donovani	76%	100%
A4HRN5	Leishmania infantum	76%	100%
A4I6F9	Leishmania infantum	25%	100%
C9ZJ17	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	100%
E9ACB3	Leishmania major	76%	99%
E9AJK1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	76%	100%
V5BCX7	Trypanosoma cruzi	48%	100%