NEDD8-activating enzyme E1 catalytic subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin activating enzyme, putative

Species:

Leishmania braziliensis

UniProt:

A4H389_LEIBR

TriTrypDb:

LbrM.01.0690 , LBRM2903_010012600

Length:

543

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H389

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H389

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0019538	protein metabolic process	3	11
GO:0032446	protein modification by small protein conjugation	6	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0045116	protein neddylation	7	11
GO:0070647	protein modification by small protein conjugation or removal	5	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	11
GO:0016874	ligase activity	2	11
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	11
GO:0017076	purine nucleotide binding	4	11
GO:0019781	NEDD8 activating enzyme activity	3	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:0140657	ATP-dependent activity	1	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0016740	transferase activity	2	1
GO:0016746	acyltransferase activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	299	303	PF00656	0.331
CLV_C14_Caspase3-7	489	493	PF00656	0.418
CLV_NRD_NRD_1	145	147	PF00675	0.305
CLV_NRD_NRD_1	149	151	PF00675	0.305
CLV_NRD_NRD_1	281	283	PF00675	0.429
CLV_NRD_NRD_1	436	438	PF00675	0.498
CLV_PCSK_KEX2_1	145	147	PF00082	0.305
CLV_PCSK_KEX2_1	281	283	PF00082	0.429
CLV_PCSK_KEX2_1	436	438	PF00082	0.498
CLV_PCSK_PC7_1	277	283	PF00082	0.450
CLV_PCSK_SKI1_1	158	162	PF00082	0.197
CLV_PCSK_SKI1_1	369	373	PF00082	0.305
CLV_PCSK_SKI1_1	8	12	PF00082	0.514
CLV_PCSK_SKI1_1	84	88	PF00082	0.305
DEG_APCC_DBOX_1	435	443	PF00400	0.562
DEG_MDM2_SWIB_1	506	514	PF02201	0.378
DOC_CKS1_1	159	164	PF01111	0.389
DOC_CYCLIN_yClb1_LxF_4	6	11	PF00134	0.602
DOC_MAPK_DCC_7	346	354	PF00069	0.305
DOC_MAPK_DCC_7	38	48	PF00069	0.466
DOC_MAPK_gen_1	436	442	PF00069	0.346
DOC_MAPK_MEF2A_6	130	139	PF00069	0.290
DOC_MAPK_MEF2A_6	346	354	PF00069	0.305
DOC_MAPK_MEF2A_6	455	462	PF00069	0.606
DOC_PP1_RVXF_1	6	12	PF00149	0.592
DOC_PP4_FxxP_1	42	45	PF00568	0.456
DOC_USP7_MATH_1	110	114	PF00917	0.451
DOC_USP7_MATH_1	138	142	PF00917	0.324
DOC_USP7_MATH_1	472	476	PF00917	0.437
DOC_USP7_MATH_1	528	532	PF00917	0.541
DOC_WW_Pin1_4	158	163	PF00397	0.415
DOC_WW_Pin1_4	165	170	PF00397	0.369
DOC_WW_Pin1_4	182	187	PF00397	0.137
DOC_WW_Pin1_4	20	25	PF00397	0.393
DOC_WW_Pin1_4	226	231	PF00397	0.324
LIG_14-3-3_CanoR_1	173	181	PF00244	0.431
LIG_14-3-3_CanoR_1	245	249	PF00244	0.275
LIG_14-3-3_CanoR_1	529	536	PF00244	0.592
LIG_14-3-3_CanoR_1	8	18	PF00244	0.505
LIG_BIR_II_1	1	5	PF00653	0.563
LIG_BRCT_BRCA1_1	24	28	PF00533	0.450
LIG_Clathr_ClatBox_1	136	140	PF01394	0.417
LIG_deltaCOP1_diTrp_1	39	42	PF00928	0.417
LIG_FHA_1	113	119	PF00498	0.388
LIG_FHA_1	155	161	PF00498	0.380
LIG_FHA_1	227	233	PF00498	0.303
LIG_FHA_1	245	251	PF00498	0.376
LIG_FHA_1	339	345	PF00498	0.364
LIG_FHA_2	392	398	PF00498	0.450
LIG_FHA_2	522	528	PF00498	0.405
LIG_FHA_2	68	74	PF00498	0.305
LIG_FHA_2	87	93	PF00498	0.305
LIG_FXI_DFP_1	129	133	PF00024	0.197
LIG_LIR_Apic_2	39	45	PF02991	0.456
LIG_LIR_Gen_1	131	139	PF02991	0.428
LIG_LIR_Gen_1	39	48	PF02991	0.309
LIG_LIR_Gen_1	64	74	PF02991	0.305
LIG_LIR_Gen_1	75	82	PF02991	0.305
LIG_LIR_Nem_3	126	132	PF02991	0.305
LIG_LIR_Nem_3	39	43	PF02991	0.303
LIG_LIR_Nem_3	64	69	PF02991	0.305
LIG_LIR_Nem_3	73	79	PF02991	0.305
LIG_NRBOX	438	444	PF00104	0.535
LIG_NRBOX	56	62	PF00104	0.417
LIG_PDZ_Class_3	538	543	PF00595	0.430
LIG_Pex14_2	128	132	PF04695	0.305
LIG_Pex14_2	506	510	PF04695	0.575
LIG_PTB_Apo_2	377	384	PF02174	0.378
LIG_SH2_NCK_1	393	397	PF00017	0.352
LIG_SH2_PTP2	266	269	PF00017	0.450
LIG_SH2_STAT5	199	202	PF00017	0.446
LIG_SH2_STAT5	237	240	PF00017	0.417
LIG_SH2_STAT5	266	269	PF00017	0.324
LIG_SH2_STAT5	358	361	PF00017	0.417
LIG_SH2_STAT5	393	396	PF00017	0.339
LIG_SH3_3	156	162	PF00018	0.423
LIG_SH3_3	183	189	PF00018	0.197
LIG_SH3_3	251	257	PF00018	0.309
LIG_SH3_3	303	309	PF00018	0.352
LIG_SH3_3	413	419	PF00018	0.440
LIG_SH3_3	466	472	PF00018	0.629
LIG_SUMO_SIM_anti_2	438	446	PF11976	0.351
LIG_SUMO_SIM_anti_2	457	464	PF11976	0.367
LIG_SUMO_SIM_par_1	135	141	PF11976	0.324
LIG_SUMO_SIM_par_1	421	426	PF11976	0.397
LIG_SUMO_SIM_par_1	438	446	PF11976	0.553
LIG_SUMO_SIM_par_1	457	464	PF11976	0.367
LIG_SUMO_SIM_par_1	59	64	PF11976	0.316
LIG_TYR_ITIM	264	269	PF00017	0.450
LIG_UBA3_1	339	346	PF00899	0.319
MOD_CK1_1	23	29	PF00069	0.303
MOD_CK1_1	241	247	PF00069	0.360
MOD_CK1_1	428	434	PF00069	0.597
MOD_CK1_1	488	494	PF00069	0.578
MOD_CK2_1	391	397	PF00069	0.450
MOD_CK2_1	535	541	PF00069	0.411
MOD_DYRK1A_RPxSP_1	158	162	PF00069	0.197
MOD_GlcNHglycan	165	168	PF01048	0.411
MOD_GlcNHglycan	240	243	PF01048	0.197
MOD_GlcNHglycan	406	409	PF01048	0.657
MOD_GlcNHglycan	426	430	PF01048	0.301
MOD_GlcNHglycan	463	466	PF01048	0.646
MOD_GlcNHglycan	474	477	PF01048	0.626
MOD_GlcNHglycan	530	533	PF01048	0.589
MOD_GlcNHglycan	63	66	PF01048	0.305
MOD_GSK3_1	154	161	PF00069	0.311
MOD_GSK3_1	486	493	PF00069	0.678
MOD_N-GLC_1	226	231	PF02516	0.417
MOD_N-GLC_1	486	491	PF02516	0.441
MOD_NEK2_1	238	243	PF00069	0.311
MOD_NEK2_1	61	66	PF00069	0.305
MOD_NEK2_1	86	91	PF00069	0.417
MOD_NEK2_1	9	14	PF00069	0.625
MOD_PIKK_1	23	29	PF00454	0.305
MOD_PIKK_1	281	287	PF00454	0.423
MOD_PIKK_1	9	15	PF00454	0.625
MOD_PKA_1	281	287	PF00069	0.450
MOD_PKA_2	172	178	PF00069	0.431
MOD_PKA_2	244	250	PF00069	0.305
MOD_PKA_2	280	286	PF00069	0.448
MOD_PKA_2	454	460	PF00069	0.372
MOD_PKA_2	528	534	PF00069	0.610
MOD_Plk_1	38	44	PF00069	0.333
MOD_Plk_1	425	431	PF00069	0.620
MOD_Plk_4	249	255	PF00069	0.374
MOD_Plk_4	454	460	PF00069	0.494
MOD_Plk_4	493	499	PF00069	0.586
MOD_Plk_4	67	73	PF00069	0.417
MOD_ProDKin_1	158	164	PF00069	0.415
MOD_ProDKin_1	165	171	PF00069	0.369
MOD_ProDKin_1	182	188	PF00069	0.137
MOD_ProDKin_1	20	26	PF00069	0.389
MOD_ProDKin_1	226	232	PF00069	0.324
MOD_SUMO_rev_2	198	207	PF00179	0.430
TRG_DiLeu_BaEn_1	202	207	PF01217	0.450
TRG_DiLeu_BaEn_1	438	443	PF01217	0.334
TRG_DiLeu_BaEn_1	56	61	PF01217	0.381
TRG_ENDOCYTIC_2	129	132	PF00928	0.297
TRG_ENDOCYTIC_2	266	269	PF00928	0.305
TRG_ENDOCYTIC_2	393	396	PF00928	0.427
TRG_ER_diArg_1	145	147	PF00400	0.305
TRG_ER_diArg_1	435	437	PF00400	0.496
TRG_Pf-PMV_PEXEL_1	437	441	PF00026	0.462

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIU8	Leptomonas seymouri	63%	100%
A0A1X0P2L8	Trypanosomatidae	44%	100%
A0A3S7WNL1	Leishmania donovani	82%	100%
A0A422NGT4	Trypanosoma rangeli	45%	100%
A4HRJ4	Leishmania infantum	82%	100%
C9ZXM5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E9AC62	Leishmania major	80%	100%
E9AJF8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
O65041	Arabidopsis thaliana	33%	100%
Q09765	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	100%
Q54QG9	Dictyostelium discoideum	31%	100%
Q5R4A0	Pongo abelii	31%	100%
Q8C878	Mus musculus	30%	100%
Q8TBC4	Homo sapiens	30%	100%
Q99MI7	Rattus norvegicus	30%	100%
Q9V6U8	Drosophila melanogaster	31%	100%
V5BH30	Trypanosoma cruzi	47%	100%