Thymidylate_kin domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

UMP-CMP kinase, mitochondrial, putative

Species:

Leishmania braziliensis

UniProt:

A4H324_LEIBR

TriTrypDb:

LbrM.01.0120 , LBRM2903_010005800

Length:

272

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A4H324

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A4H324

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006220	pyrimidine nucleotide metabolic process	5	1
GO:0006221	pyrimidine nucleotide biosynthetic process	6	1
GO:0006227	dUDP biosynthetic process	8	1
GO:0006233	dTDP biosynthetic process	8	1
GO:0006235	dTTP biosynthetic process	8	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006753	nucleoside phosphate metabolic process	4	1
GO:0006793	phosphorus metabolic process	3	4
GO:0006796	phosphate-containing compound metabolic process	4	4
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	4
GO:0009058	biosynthetic process	2	1
GO:0009117	nucleotide metabolic process	5	1
GO:0009132	nucleoside diphosphate metabolic process	5	1
GO:0009133	nucleoside diphosphate biosynthetic process	6	1
GO:0009138	pyrimidine nucleoside diphosphate metabolic process	6	1
GO:0009139	pyrimidine nucleoside diphosphate biosynthetic process	7	1
GO:0009141	nucleoside triphosphate metabolic process	5	1
GO:0009142	nucleoside triphosphate biosynthetic process	6	1
GO:0009147	pyrimidine nucleoside triphosphate metabolic process	6	1
GO:0009148	pyrimidine nucleoside triphosphate biosynthetic process	7	1
GO:0009165	nucleotide biosynthetic process	6	1
GO:0009186	deoxyribonucleoside diphosphate metabolic process	6	1
GO:0009189	deoxyribonucleoside diphosphate biosynthetic process	7	1
GO:0009196	pyrimidine deoxyribonucleoside diphosphate metabolic process	7	1
GO:0009197	pyrimidine deoxyribonucleoside diphosphate biosynthetic process	8	1
GO:0009211	pyrimidine deoxyribonucleoside triphosphate metabolic process	7	1
GO:0009212	pyrimidine deoxyribonucleoside triphosphate biosynthetic process	8	1
GO:0009219	pyrimidine deoxyribonucleotide metabolic process	6	1
GO:0009221	pyrimidine deoxyribonucleotide biosynthetic process	7	1
GO:0009262	deoxyribonucleotide metabolic process	4	1
GO:0009263	deoxyribonucleotide biosynthetic process	5	1
GO:0009265	2'-deoxyribonucleotide biosynthetic process	6	1
GO:0009394	2'-deoxyribonucleotide metabolic process	5	1
GO:0009987	cellular process	1	4
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0019637	organophosphate metabolic process	3	1
GO:0019692	deoxyribose phosphate metabolic process	4	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0044281	small molecule metabolic process	2	1
GO:0046072	dTDP metabolic process	7	1
GO:0046075	dTTP metabolic process	7	1
GO:0046077	dUDP metabolic process	7	1
GO:0046385	deoxyribose phosphate biosynthetic process	5	1
GO:0046483	heterocycle metabolic process	3	1
GO:0055086	nucleobase-containing small molecule metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0072527	pyrimidine-containing compound metabolic process	4	1
GO:0072528	pyrimidine-containing compound biosynthetic process	5	1
GO:0090407	organophosphate biosynthetic process	4	1
GO:1901135	carbohydrate derivative metabolic process	3	1
GO:1901137	carbohydrate derivative biosynthetic process	4	1
GO:1901293	nucleoside phosphate biosynthetic process	5	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1
GO:0016310	phosphorylation	5	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0016301	kinase activity	4	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016776	phosphotransferase activity, phosphate group as acceptor	4	10
GO:0019205	nucleobase-containing compound kinase activity	5	10
GO:0050145	nucleoside monophosphate kinase activity	5	10
GO:0004127	cytidylate kinase activity	6	2
GO:0004550	nucleoside diphosphate kinase activity	5	1
GO:0004798	thymidylate kinase activity	6	1
GO:0009041	uridylate kinase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	20	24	PF00656	0.261
CLV_C14_Caspase3-7	71	75	PF00656	0.271
CLV_NRD_NRD_1	197	199	PF00675	0.298
CLV_NRD_NRD_1	217	219	PF00675	0.363
CLV_NRD_NRD_1	225	227	PF00675	0.329
CLV_NRD_NRD_1	63	65	PF00675	0.598
CLV_PCSK_KEX2_1	199	201	PF00082	0.304
CLV_PCSK_KEX2_1	217	219	PF00082	0.361
CLV_PCSK_KEX2_1	225	227	PF00082	0.329
CLV_PCSK_KEX2_1	63	65	PF00082	0.559
CLV_PCSK_PC1ET2_1	199	201	PF00082	0.234
DEG_APCC_DBOX_1	18	26	PF00400	0.405
DEG_SCF_FBW7_1	37	44	PF00400	0.431
DOC_CKS1_1	38	43	PF01111	0.365
DOC_CKS1_1	94	99	PF01111	0.271
DOC_MAPK_gen_1	19	27	PF00069	0.418
DOC_MAPK_gen_1	63	70	PF00069	0.427
DOC_MAPK_MEF2A_6	63	70	PF00069	0.494
DOC_PP1_SILK_1	243	248	PF00149	0.358
DOC_PP2B_LxvP_1	177	180	PF13499	0.475
DOC_PP2B_PxIxI_1	65	71	PF00149	0.331
DOC_USP7_MATH_1	134	138	PF00917	0.400
DOC_USP7_MATH_1	241	245	PF00917	0.435
DOC_USP7_MATH_1	41	45	PF00917	0.408
DOC_WW_Pin1_4	32	37	PF00397	0.414
DOC_WW_Pin1_4	93	98	PF00397	0.276
LIG_14-3-3_CanoR_1	117	126	PF00244	0.250
LIG_14-3-3_CanoR_1	144	154	PF00244	0.271
LIG_14-3-3_CanoR_1	209	216	PF00244	0.350
LIG_CAP-Gly_1	265	272	PF01302	0.456
LIG_Clathr_ClatBox_1	185	189	PF01394	0.289
LIG_CSL_BTD_1	38	41	PF09270	0.371
LIG_DLG_GKlike_1	21	28	PF00625	0.253
LIG_EVH1_2	97	101	PF00568	0.438
LIG_FHA_1	225	231	PF00498	0.381
LIG_FHA_1	235	241	PF00498	0.280
LIG_FHA_1	80	86	PF00498	0.323
LIG_FHA_2	260	266	PF00498	0.393
LIG_FHA_2	82	88	PF00498	0.436
LIG_LIR_Apic_2	168	174	PF02991	0.292
LIG_LIR_Apic_2	35	41	PF02991	0.313
LIG_PDZ_Class_3	267	272	PF00595	0.452
LIG_SH2_CRK	160	164	PF00017	0.384
LIG_SH2_NCK_1	120	124	PF00017	0.289
LIG_SH2_STAT5	125	128	PF00017	0.275
LIG_SH3_3	230	236	PF00018	0.349
LIG_SH3_3	91	97	PF00018	0.284
LIG_SUMO_SIM_anti_2	137	143	PF11976	0.394
LIG_SUMO_SIM_par_1	137	143	PF11976	0.338
LIG_SUMO_SIM_par_1	65	71	PF11976	0.334
LIG_TRAF2_1	110	113	PF00917	0.228
MOD_CK1_1	211	217	PF00069	0.384
MOD_CK1_1	42	48	PF00069	0.345
MOD_CK1_1	52	58	PF00069	0.525
MOD_CK2_1	107	113	PF00069	0.390
MOD_CK2_1	209	215	PF00069	0.455
MOD_CK2_1	259	265	PF00069	0.368
MOD_CK2_1	81	87	PF00069	0.314
MOD_GlcNHglycan	120	123	PF01048	0.314
MOD_GlcNHglycan	136	139	PF01048	0.289
MOD_GlcNHglycan	165	168	PF01048	0.313
MOD_GlcNHglycan	251	254	PF01048	0.419
MOD_GlcNHglycan	57	60	PF01048	0.664
MOD_GlcNHglycan	89	92	PF01048	0.483
MOD_GSK3_1	13	20	PF00069	0.366
MOD_GSK3_1	159	166	PF00069	0.477
MOD_GSK3_1	224	231	PF00069	0.423
MOD_GSK3_1	37	44	PF00069	0.366
MOD_GSK3_1	49	56	PF00069	0.378
MOD_NEK2_1	132	137	PF00069	0.335
MOD_NEK2_1	53	58	PF00069	0.649
MOD_NEK2_1	7	12	PF00069	0.478
MOD_NEK2_1	73	78	PF00069	0.370
MOD_NEK2_1	92	97	PF00069	0.473
MOD_NEK2_2	13	18	PF00069	0.274
MOD_PIKK_1	107	113	PF00454	0.353
MOD_PIKK_1	132	138	PF00454	0.335
MOD_PIKK_1	209	215	PF00454	0.313
MOD_PIKK_1	259	265	PF00454	0.362
MOD_PKA_2	104	110	PF00069	0.404
MOD_PKA_2	208	214	PF00069	0.326
MOD_PKA_2	224	230	PF00069	0.271
MOD_PKB_1	19	27	PF00069	0.262
MOD_Plk_2-3	265	271	PF00069	0.448
MOD_Plk_4	13	19	PF00069	0.318
MOD_Plk_4	241	247	PF00069	0.472
MOD_Plk_4	42	48	PF00069	0.444
MOD_Plk_4	76	82	PF00069	0.354
MOD_ProDKin_1	32	38	PF00069	0.410
MOD_ProDKin_1	93	99	PF00069	0.276
TRG_DiLeu_BaLyEn_6	244	249	PF01217	0.375
TRG_ENDOCYTIC_2	160	163	PF00928	0.335
TRG_ER_diArg_1	18	21	PF00400	0.459
TRG_ER_diArg_1	197	200	PF00400	0.273
TRG_ER_diArg_1	216	218	PF00400	0.163
TRG_ER_diArg_1	224	226	PF00400	0.255
TRG_NLS_MonoExtC_3	197	202	PF00514	0.284
TRG_NLS_MonoExtN_4	196	202	PF00514	0.284
TRG_Pf-PMV_PEXEL_1	247	251	PF00026	0.401
TRG_Pf-PMV_PEXEL_1	83	87	PF00026	0.404

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IL87	Leptomonas seymouri	65%	96%
A0A0S4JME8	Bodo saltans	44%	92%
A0A1X0P3H2	Trypanosomatidae	54%	100%
A0A3S5H4S9	Leishmania donovani	86%	100%
A0A422NYA0	Trypanosoma rangeli	52%	100%
A4HRD0	Leishmania infantum	86%	100%
E9ABZ8	Leishmania major	85%	100%
E9AJ95	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	99%
V5D316	Trypanosoma cruzi	52%	100%