Fumarate hydratase

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Fumarate hydratase

Gene product:

Fumarate hydratase class I, cytosolic

Species:

Leishmania donovani

UniProt:

A2CIN8_LEIDO

TriTrypDb:

LdBPK_292080.1 , LdCL_290026700 , LDHU3_29.2970

Length:

568

Annotations

Annotations by Jardim et al.

Tricarboxylic acid cycle, Fumarate hydratase fh

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	yes	yes: 4
Forrest at al. (procyclic)	yes	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005777	peroxisome	6	1
GO:0005829	cytosol	2	1
GO:0020015	glycosome	7	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0042579	microbody	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A2CIN8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

No structure information available for this entry

Related structures:

PDB structure(s): 5l2r_A , 5l2r_B , 6msn_A , 6msn_B , 6unz_A , 6unz_B , 6unz_C , 6unz_D , 6unz_E , 6unz_F , 6unz_G , 6unz_H , 6uo0_A , 6uo0_B , 6uoi_A , 6uoi_B , 6uoj_A , 6uoj_B , 6up9_A , 6up9_B , 6upm_A , 6upm_B , 6upo_A , 6upo_B , 6uq8_A , 6uq8_B , 6uq9_A , 6uq9_B , 6uqb_A , 6uqb_B , 6uql_A , 6uql_B , 6uqm_A , 6uqm_B , 6uqn_A , 6uqn_B

Function

Biological processes
Term	Name	Level	Count
GO:0006091	generation of precursor metabolites and energy	3	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0044237	cellular metabolic process	2	11
GO:0006082	organic acid metabolic process	3	1
GO:0006106	fumarate metabolic process	7	1
GO:0006108	malate metabolic process	7	1
GO:0019752	carboxylic acid metabolic process	5	1
GO:0043436	oxoacid metabolic process	4	1
GO:0043648	dicarboxylic acid metabolic process	6	1
GO:0044281	small molecule metabolic process	2	1
GO:0071704	organic substance metabolic process	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004333	fumarate hydratase activity	5	11
GO:0005488	binding	1	11
GO:0016829	lyase activity	2	11
GO:0016835	carbon-oxygen lyase activity	3	11
GO:0016836	hydro-lyase activity	4	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0051536	iron-sulfur cluster binding	3	11
GO:0051539	4 iron, 4 sulfur cluster binding	4	11
GO:0051540	metal cluster binding	2	11
GO:0005515	protein binding	2	1
GO:0042802	identical protein binding	3	1
GO:0042803	protein homodimerization activity	4	1
GO:0046983	protein dimerization activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	106	110	PF00656	0.260
CLV_C14_Caspase3-7	430	434	PF00656	0.392
CLV_NRD_NRD_1	12	14	PF00675	0.450
CLV_NRD_NRD_1	64	66	PF00675	0.369
CLV_NRD_NRD_1	90	92	PF00675	0.260
CLV_NRD_NRD_1	99	101	PF00675	0.227
CLV_PCSK_KEX2_1	12	14	PF00082	0.448
CLV_PCSK_KEX2_1	144	146	PF00082	0.260
CLV_PCSK_KEX2_1	392	394	PF00082	0.305
CLV_PCSK_KEX2_1	99	101	PF00082	0.251
CLV_PCSK_PC1ET2_1	144	146	PF00082	0.260
CLV_PCSK_PC1ET2_1	392	394	PF00082	0.305
CLV_PCSK_SKI1_1	228	232	PF00082	0.271
CLV_PCSK_SKI1_1	271	275	PF00082	0.246
CLV_PCSK_SKI1_1	350	354	PF00082	0.260
CLV_PCSK_SKI1_1	410	414	PF00082	0.309
CLV_PCSK_SKI1_1	496	500	PF00082	0.246
CLV_PCSK_SKI1_1	526	530	PF00082	0.327
CLV_PCSK_SKI1_1	65	69	PF00082	0.322
DEG_APCC_DBOX_1	349	357	PF00400	0.246
DOC_MAPK_RevD_3	378	393	PF00069	0.258
DOC_PP2B_LxvP_1	199	202	PF13499	0.387
DOC_PP4_FxxP_1	295	298	PF00568	0.258
DOC_USP7_MATH_1	105	109	PF00917	0.260
DOC_USP7_MATH_1	308	312	PF00917	0.279
DOC_USP7_MATH_1	460	464	PF00917	0.290
DOC_USP7_MATH_1	521	525	PF00917	0.420
DOC_USP7_UBL2_3	157	161	PF12436	0.310
DOC_USP7_UBL2_3	522	526	PF12436	0.392
DOC_WW_Pin1_4	442	447	PF00397	0.305
DOC_WW_Pin1_4	452	457	PF00397	0.217
LIG_14-3-3_CanoR_1	173	183	PF00244	0.246
LIG_14-3-3_CanoR_1	333	339	PF00244	0.246
LIG_14-3-3_CanoR_1	381	391	PF00244	0.443
LIG_14-3-3_CanoR_1	399	407	PF00244	0.234
LIG_14-3-3_CanoR_1	81	90	PF00244	0.333
LIG_APCC_ABBA_1	180	185	PF00400	0.246
LIG_APCC_ABBAyCdc20_2	220	226	PF00400	0.340
LIG_APCC_ABBAyCdc20_2	358	364	PF00400	0.340
LIG_APCC_Cbox_2	111	117	PF00515	0.246
LIG_BIR_II_1	1	5	PF00653	0.296
LIG_BRCT_BRCA1_1	31	35	PF00533	0.507
LIG_BRCT_BRCA1_1	460	464	PF00533	0.246
LIG_deltaCOP1_diTrp_1	539	549	PF00928	0.298
LIG_DLG_GKlike_1	471	478	PF00625	0.392
LIG_eIF4E_1	113	119	PF01652	0.392
LIG_FHA_1	136	142	PF00498	0.297
LIG_FHA_1	175	181	PF00498	0.256
LIG_FHA_1	225	231	PF00498	0.246
LIG_FHA_1	270	276	PF00498	0.260
LIG_FHA_1	382	388	PF00498	0.278
LIG_FHA_1	414	420	PF00498	0.271
LIG_FHA_1	442	448	PF00498	0.312
LIG_FHA_2	149	155	PF00498	0.260
LIG_FHA_2	262	268	PF00498	0.246
LIG_FHA_2	284	290	PF00498	0.340
LIG_LIR_Gen_1	111	119	PF02991	0.363
LIG_LIR_Gen_1	204	212	PF02991	0.305
LIG_LIR_Gen_1	28	37	PF02991	0.410
LIG_LIR_Gen_1	297	308	PF02991	0.260
LIG_LIR_Gen_1	45	54	PF02991	0.295
LIG_LIR_Gen_1	473	479	PF02991	0.322
LIG_LIR_Gen_1	485	490	PF02991	0.273
LIG_LIR_Nem_3	204	209	PF02991	0.305
LIG_LIR_Nem_3	28	34	PF02991	0.377
LIG_LIR_Nem_3	297	303	PF02991	0.260
LIG_LIR_Nem_3	45	50	PF02991	0.322
LIG_LIR_Nem_3	473	478	PF02991	0.342
LIG_LIR_Nem_3	485	489	PF02991	0.232
LIG_LIR_Nem_3	547	552	PF02991	0.341
LIG_PDZ_Class_1	563	568	PF00595	0.358
LIG_Pex14_2	31	35	PF04695	0.507
LIG_PTB_Apo_2	168	175	PF02174	0.392
LIG_PTB_Phospho_1	168	174	PF10480	0.392
LIG_REV1ctd_RIR_1	87	96	PF16727	0.279
LIG_RPA_C_Fungi	416	428	PF08784	0.279
LIG_SH2_CRK	169	173	PF00017	0.392
LIG_SH2_CRK	281	285	PF00017	0.246
LIG_SH2_CRK	449	453	PF00017	0.246
LIG_SH2_GRB2like	169	172	PF00017	0.311
LIG_SH2_PTP2	475	478	PF00017	0.290
LIG_SH2_PTP2	486	489	PF00017	0.362
LIG_SH2_STAP1	174	178	PF00017	0.255
LIG_SH2_STAP1	206	210	PF00017	0.392
LIG_SH2_STAP1	563	567	PF00017	0.370
LIG_SH2_STAT3	222	225	PF00017	0.340
LIG_SH2_STAT3	362	365	PF00017	0.305
LIG_SH2_STAT5	113	116	PF00017	0.392
LIG_SH2_STAT5	158	161	PF00017	0.334
LIG_SH2_STAT5	222	225	PF00017	0.340
LIG_SH2_STAT5	321	324	PF00017	0.298
LIG_SH2_STAT5	362	365	PF00017	0.314
LIG_SH2_STAT5	373	376	PF00017	0.194
LIG_SH2_STAT5	475	478	PF00017	0.271
LIG_SH2_STAT5	486	489	PF00017	0.343
LIG_SH2_STAT5	509	512	PF00017	0.246
LIG_SH2_STAT5	56	59	PF00017	0.369
LIG_SH3_3	34	40	PF00018	0.371
LIG_SH3_3	372	378	PF00018	0.297
LIG_SH3_5	202	206	PF00018	0.258
LIG_SUMO_SIM_anti_2	73	79	PF11976	0.381
LIG_UBA3_1	352	358	PF00899	0.369
MOD_CK1_1	283	289	PF00069	0.260
MOD_CK1_1	383	389	PF00069	0.324
MOD_CK1_1	485	491	PF00069	0.236
MOD_CK2_1	148	154	PF00069	0.260
MOD_CK2_1	201	207	PF00069	0.389
MOD_CK2_1	261	267	PF00069	0.246
MOD_CK2_1	521	527	PF00069	0.246
MOD_Cter_Amidation	142	145	PF01082	0.260
MOD_Cter_Amidation	63	66	PF01082	0.306
MOD_GlcNHglycan	20	25	PF01048	0.369
MOD_GlcNHglycan	216	219	PF01048	0.232
MOD_GlcNHglycan	264	267	PF01048	0.246
MOD_GlcNHglycan	31	34	PF01048	0.436
MOD_GlcNHglycan	310	313	PF01048	0.288
MOD_GlcNHglycan	346	349	PF01048	0.246
MOD_GlcNHglycan	358	361	PF01048	0.339
MOD_GlcNHglycan	460	463	PF01048	0.249
MOD_GSK3_1	129	136	PF00069	0.361
MOD_GSK3_1	262	269	PF00069	0.246
MOD_GSK3_1	280	287	PF00069	0.273
MOD_GSK3_1	460	467	PF00069	0.290
MOD_GSK3_1	478	485	PF00069	0.130
MOD_GSK3_1	492	499	PF00069	0.246
MOD_N-GLC_1	356	361	PF02516	0.343
MOD_N-GLC_1	413	418	PF02516	0.392
MOD_N-GLC_1	492	497	PF02516	0.246
MOD_N-GLC_2	121	123	PF02516	0.260
MOD_NEK2_1	148	153	PF00069	0.279
MOD_NEK2_1	172	177	PF00069	0.246
MOD_NEK2_1	29	34	PF00069	0.408
MOD_NEK2_1	342	347	PF00069	0.260
MOD_NEK2_1	382	387	PF00069	0.430
MOD_NEK2_1	413	418	PF00069	0.253
MOD_NEK2_1	478	483	PF00069	0.305
MOD_NEK2_2	266	271	PF00069	0.246
MOD_NEK2_2	521	526	PF00069	0.392
MOD_PIKK_1	172	178	PF00454	0.246
MOD_PIKK_1	400	406	PF00454	0.279
MOD_PKA_2	172	178	PF00069	0.246
MOD_PKA_2	380	386	PF00069	0.200
MOD_Plk_1	20	26	PF00069	0.442
MOD_Plk_1	266	272	PF00069	0.256
MOD_Plk_1	526	532	PF00069	0.200
MOD_Plk_4	114	120	PF00069	0.260
MOD_Plk_4	413	419	PF00069	0.260
MOD_Plk_4	471	477	PF00069	0.255
MOD_Plk_4	482	488	PF00069	0.226
MOD_Plk_4	73	79	PF00069	0.332
MOD_ProDKin_1	442	448	PF00069	0.305
MOD_ProDKin_1	452	458	PF00069	0.217
MOD_SUMO_for_1	48	51	PF00179	0.431
MOD_SUMO_rev_2	154	159	PF00179	0.279
MOD_SUMO_rev_2	235	244	PF00179	0.278
MOD_SUMO_rev_2	386	394	PF00179	0.305
TRG_DiLeu_BaEn_1	226	231	PF01217	0.392
TRG_ENDOCYTIC_2	113	116	PF00928	0.246
TRG_ENDOCYTIC_2	169	172	PF00928	0.426
TRG_ENDOCYTIC_2	206	209	PF00928	0.280
TRG_ENDOCYTIC_2	281	284	PF00928	0.246
TRG_ENDOCYTIC_2	475	478	PF00928	0.400
TRG_ENDOCYTIC_2	486	489	PF00928	0.440
TRG_ER_diArg_1	98	100	PF00400	0.251
TRG_NES_CRM1_1	190	205	PF08389	0.313
TRG_Pf-PMV_PEXEL_1	100	104	PF00026	0.249
TRG_Pf-PMV_PEXEL_1	393	397	PF00026	0.250

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I631	Leptomonas seymouri	87%	100%
A0A0N1PCN7	Leptomonas seymouri	65%	100%
A0A1X0NIU7	Trypanosomatidae	63%	100%
A0A1X0NZZ8	Trypanosomatidae	76%	100%
A0A3R7N408	Trypanosoma rangeli	62%	100%
A0A3S5IRI9	Trypanosoma rangeli	74%	100%
A0A3S7WY69	Leishmania donovani	60%	100%
A2CIN0	Leishmania infantum	100%	100%
A4HD88	Leishmania braziliensis	63%	100%
A4HHJ6	Leishmania braziliensis	92%	100%
C9ZLE3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	72%	100%
D0A701	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	100%
E9AE57	Leishmania major	98%	100%
E9AH43	Leishmania infantum	60%	100%
E9AWM1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	59%	100%
P0AC33	Escherichia coli (strain K12)	64%	100%
P0AC34	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	64%	100%
P14407	Escherichia coli (strain K12)	66%	100%
P40720	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	66%	98%
Q04718	Geobacillus stearothermophilus	28%	100%
Q4QAU9	Leishmania major	59%	100%
Q8X4P8	Escherichia coli O157:H7	65%	100%
V5BGH8	Trypanosoma cruzi	74%	100%
V5DSN1	Trypanosoma cruzi	66%	100%