Ubiquitin thioesterase OTU1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

OTU-like cysteine protease, putative

Species:

Leishmania donovani

UniProt:

A0A3S7XC16_LEIDO

TriTrypDb:

LdBPK_366280.1 , LdCL_360070200 , LDHU3_36.8330

Length:

385

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A0A3S7XC16

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7XC16

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	12
GO:0006511	ubiquitin-dependent protein catabolic process	7	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0008152	metabolic process	1	12
GO:0009056	catabolic process	2	12
GO:0009057	macromolecule catabolic process	4	12
GO:0009987	cellular process	1	12
GO:0010033	response to organic substance	3	12
GO:0010243	response to organonitrogen compound	4	12
GO:0010498	proteasomal protein catabolic process	5	12
GO:0016579	protein deubiquitination	6	12
GO:0019538	protein metabolic process	3	12
GO:0019941	modification-dependent protein catabolic process	6	12
GO:0030163	protein catabolic process	4	12
GO:0030433	ubiquitin-dependent ERAD pathway	6	12
GO:0033554	cellular response to stress	3	12
GO:0034976	response to endoplasmic reticulum stress	4	12
GO:0036211	protein modification process	4	12
GO:0036503	ERAD pathway	5	12
GO:0042221	response to chemical	2	12
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043632	modification-dependent macromolecule catabolic process	5	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044248	cellular catabolic process	3	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0044265	obsolete cellular macromolecule catabolic process	4	12
GO:0050896	response to stimulus	1	12
GO:0051603	proteolysis involved in protein catabolic process	5	12
GO:0051716	cellular response to stimulus	2	12
GO:0070646	protein modification by small protein removal	5	12
GO:0070647	protein modification by small protein conjugation or removal	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901565	organonitrogen compound catabolic process	4	12
GO:1901575	organic substance catabolic process	3	12
GO:1901698	response to nitrogen compound	3	12
GO:0007165	signal transduction	2	1
GO:0030968	endoplasmic reticulum unfolded protein response	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0065007	biological regulation	1	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004843	cysteine-type deubiquitinase activity	5	12
GO:0008233	peptidase activity	3	12
GO:0008234	cysteine-type peptidase activity	4	12
GO:0016787	hydrolase activity	2	12
GO:0019783	ubiquitin-like protein peptidase activity	4	12
GO:0101005	deubiquitinase activity	5	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	88	94	PF00089	0.428
CLV_NRD_NRD_1	151	153	PF00675	0.485
CLV_NRD_NRD_1	222	224	PF00675	0.381
CLV_NRD_NRD_1	67	69	PF00675	0.502
CLV_PCSK_FUR_1	367	371	PF00082	0.469
CLV_PCSK_KEX2_1	221	223	PF00082	0.391
CLV_PCSK_KEX2_1	369	371	PF00082	0.460
CLV_PCSK_PC1ET2_1	369	371	PF00082	0.489
CLV_PCSK_SKI1_1	326	330	PF00082	0.402
CLV_PCSK_SKI1_1	54	58	PF00082	0.446
CLV_Separin_Metazoa	49	53	PF03568	0.421
DEG_SCF_FBW7_1	124	130	PF00400	0.593
DEG_SPOP_SBC_1	127	131	PF00917	0.631
DOC_CKS1_1	124	129	PF01111	0.581
DOC_CYCLIN_RxL_1	6	18	PF00134	0.413
DOC_CYCLIN_yCln2_LP_2	22	28	PF00134	0.408
DOC_MAPK_gen_1	152	158	PF00069	0.485
DOC_MAPK_gen_1	184	193	PF00069	0.481
DOC_SPAK_OSR1_1	91	95	PF12202	0.449
DOC_USP7_MATH_1	127	131	PF00917	0.542
DOC_USP7_MATH_1	226	230	PF00917	0.490
DOC_USP7_MATH_1	276	280	PF00917	0.431
DOC_WW_Pin1_4	123	128	PF00397	0.573
DOC_WW_Pin1_4	175	180	PF00397	0.561
LIG_14-3-3_CanoR_1	125	135	PF00244	0.499
LIG_14-3-3_CanoR_1	152	158	PF00244	0.582
LIG_14-3-3_CanoR_1	42	51	PF00244	0.433
LIG_14-3-3_CanoR_1	54	64	PF00244	0.370
LIG_14-3-3_CanoR_1	68	73	PF00244	0.385
LIG_14-3-3_CanoR_1	91	95	PF00244	0.449
LIG_BRCT_BRCA1_1	228	232	PF00533	0.331
LIG_EVH1_1	210	214	PF00568	0.375
LIG_FHA_1	16	22	PF00498	0.537
LIG_FHA_1	30	36	PF00498	0.486
LIG_FHA_1	45	51	PF00498	0.421
LIG_FHA_1	9	15	PF00498	0.646
LIG_FHA_2	106	112	PF00498	0.470
LIG_FHA_2	140	146	PF00498	0.533
LIG_LIR_Apic_2	207	212	PF02991	0.503
LIG_LIR_Gen_1	17	26	PF02991	0.567
LIG_LIR_Gen_1	58	66	PF02991	0.616
LIG_LIR_Gen_1	89	100	PF02991	0.469
LIG_LIR_Nem_3	17	22	PF02991	0.569
LIG_LIR_Nem_3	58	63	PF02991	0.585
LIG_LIR_Nem_3	89	95	PF02991	0.456
LIG_PCNA_yPIPBox_3	251	259	PF02747	0.522
LIG_Pex14_2	60	64	PF04695	0.437
LIG_PTB_Apo_2	339	346	PF02174	0.425
LIG_PTB_Phospho_1	339	345	PF10480	0.441
LIG_SH2_CRK	336	340	PF00017	0.326
LIG_SH2_NCK_1	336	340	PF00017	0.326
LIG_SH2_SRC	209	212	PF00017	0.474
LIG_SH2_STAP1	277	281	PF00017	0.441
LIG_SH2_STAP1	323	327	PF00017	0.338
LIG_SH2_STAP1	336	340	PF00017	0.326
LIG_SH2_STAT5	209	212	PF00017	0.474
LIG_SH2_STAT5	237	240	PF00017	0.339
LIG_SH2_STAT5	323	326	PF00017	0.346
LIG_SH2_STAT5	331	334	PF00017	0.336
LIG_SH2_STAT5	36	39	PF00017	0.429
LIG_SH3_1	208	214	PF00018	0.619
LIG_SH3_2	211	216	PF14604	0.616
LIG_SH3_3	208	214	PF00018	0.619
LIG_SH3_3	341	347	PF00018	0.325
LIG_SH3_3	69	75	PF00018	0.585
LIG_SUMO_SIM_anti_2	292	297	PF11976	0.339
LIG_SUMO_SIM_par_1	189	195	PF11976	0.442
LIG_UBA3_1	295	301	PF00899	0.446
LIG_UBA3_1	63	69	PF00899	0.630
LIG_WRC_WIRS_1	16	21	PF05994	0.411
LIG_WRC_WIRS_1	295	300	PF05994	0.347
LIG_WRC_WIRS_1	57	62	PF05994	0.568
MOD_CDK_SPK_2	123	128	PF00069	0.573
MOD_CK1_1	139	145	PF00069	0.497
MOD_CK1_1	151	157	PF00069	0.392
MOD_CK1_1	178	184	PF00069	0.488
MOD_CK1_1	279	285	PF00069	0.545
MOD_CK1_1	93	99	PF00069	0.460
MOD_CK2_1	105	111	PF00069	0.643
MOD_CK2_1	127	133	PF00069	0.539
MOD_CK2_1	139	145	PF00069	0.521
MOD_CK2_1	240	246	PF00069	0.550
MOD_CK2_1	346	352	PF00069	0.460
MOD_CK2_1	356	362	PF00069	0.440
MOD_CK2_1	43	49	PF00069	0.417
MOD_GlcNHglycan	139	142	PF01048	0.528
MOD_GlcNHglycan	180	183	PF01048	0.490
MOD_GSK3_1	123	130	PF00069	0.543
MOD_GSK3_1	279	286	PF00069	0.500
MOD_GSK3_1	352	359	PF00069	0.512
MOD_GSK3_1	4	11	PF00069	0.622
MOD_GSK3_1	99	106	PF00069	0.549
MOD_N-GLC_1	137	142	PF02516	0.534
MOD_N-GLC_2	228	230	PF02516	0.343
MOD_NEK2_1	1	6	PF00069	0.453
MOD_NEK2_1	118	123	PF00069	0.492
MOD_NEK2_1	137	142	PF00069	0.496
MOD_NEK2_1	14	19	PF00069	0.374
MOD_NEK2_1	321	326	PF00069	0.436
MOD_NEK2_1	329	334	PF00069	0.463
MOD_NEK2_1	43	48	PF00069	0.421
MOD_NEK2_2	90	95	PF00069	0.446
MOD_OFUCOSY	224	230	PF10250	0.387
MOD_PIKK_1	29	35	PF00454	0.406
MOD_PIKK_1	4	10	PF00454	0.417
MOD_PIKK_1	99	105	PF00454	0.478
MOD_PK_1	68	74	PF00069	0.434
MOD_PKA_1	68	74	PF00069	0.434
MOD_PKA_2	151	157	PF00069	0.476
MOD_PKA_2	90	96	PF00069	0.451
MOD_Plk_1	226	232	PF00069	0.353
MOD_Plk_1	352	358	PF00069	0.497
MOD_Plk_4	276	282	PF00069	0.472
MOD_Plk_4	294	300	PF00069	0.258
MOD_Plk_4	352	358	PF00069	0.497
MOD_Plk_4	56	62	PF00069	0.504
MOD_ProDKin_1	123	129	PF00069	0.578
MOD_ProDKin_1	175	181	PF00069	0.562
MOD_SUMO_rev_2	195	200	PF00179	0.626
MOD_SUMO_rev_2	375	385	PF00179	0.491
TRG_DiLeu_BaEn_1	145	150	PF01217	0.551
TRG_DiLeu_BaEn_2	293	299	PF01217	0.448
TRG_ENDOCYTIC_2	336	339	PF00928	0.329
TRG_ER_diArg_1	122	125	PF00400	0.401
TRG_ER_diArg_1	160	163	PF00400	0.452
TRG_ER_diArg_1	221	223	PF00400	0.404
TRG_ER_diArg_1	51	54	PF00400	0.420
TRG_NES_CRM1_1	263	273	PF08389	0.420

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P901	Leptomonas seymouri	76%	100%
A0A0S4IPY5	Bodo saltans	47%	100%
A0A1X0NNB5	Trypanosomatidae	50%	100%
A0A3R7LXZ2	Trypanosoma rangeli	54%	100%
A4HQC1	Leishmania braziliensis	76%	100%
A4IE15	Leishmania infantum	100%	100%
D0A3Y1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	100%
E9AU37	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
O13974	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
Q4Q0F8	Leishmania major	81%	97%
Q55BI3	Dictyostelium discoideum	32%	100%
Q9VRJ9	Drosophila melanogaster	29%	100%
V5B3J2	Trypanosoma cruzi	53%	100%