Asparaginase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Asparaginase

Gene product:

asparaginase-like protein

Species:

Leishmania donovani

UniProt:

A0A3S7XBI7_LEIDO

TriTrypDb:

LdBPK_364650.1 , LdCL_360053600 , LDHU3_36.6270

Length:

369

Annotations

Annotations by Jardim et al.

Amino acid metabolism, Asparaginase-like

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3S7XBI7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7XBI7

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006520	amino acid metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009066	aspartate family amino acid metabolic process	5	12
GO:0009987	cellular process	1	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901605	alpha-amino acid metabolic process	4	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004067	asparaginase activity	5	12
GO:0016787	hydrolase activity	2	12
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	3	12
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	4	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	114	118	PF00656	0.426
CLV_NRD_NRD_1	10	12	PF00675	0.705
CLV_NRD_NRD_1	215	217	PF00675	0.360
CLV_NRD_NRD_1	277	279	PF00675	0.313
CLV_NRD_NRD_1	350	352	PF00675	0.453
CLV_NRD_NRD_1	53	55	PF00675	0.520
CLV_PCSK_KEX2_1	277	279	PF00082	0.335
CLV_PCSK_KEX2_1	282	284	PF00082	0.326
CLV_PCSK_KEX2_1	52	54	PF00082	0.551
CLV_PCSK_PC1ET2_1	282	284	PF00082	0.381
CLV_PCSK_PC7_1	278	284	PF00082	0.337
CLV_PCSK_SKI1_1	185	189	PF00082	0.319
CLV_PCSK_SKI1_1	216	220	PF00082	0.402
CLV_PCSK_SKI1_1	237	241	PF00082	0.404
CLV_PCSK_SKI1_1	28	32	PF00082	0.458
CLV_PCSK_SKI1_1	282	286	PF00082	0.351
CLV_PCSK_SKI1_1	338	342	PF00082	0.322
CLV_PCSK_SKI1_1	53	57	PF00082	0.462
DEG_APCC_DBOX_1	53	61	PF00400	0.515
DEG_Nend_Nbox_1	1	3	PF02207	0.622
DEG_SPOP_SBC_1	120	124	PF00917	0.391
DOC_CDC14_PxL_1	258	266	PF14671	0.337
DOC_CYCLIN_yCln2_LP_2	133	139	PF00134	0.322
DOC_MAPK_gen_1	289	297	PF00069	0.372
DOC_MAPK_gen_1	52	59	PF00069	0.490
DOC_MAPK_MEF2A_6	155	163	PF00069	0.307
DOC_MAPK_MEF2A_6	289	297	PF00069	0.415
DOC_MAPK_RevD_3	203	217	PF00069	0.278
DOC_PP1_RVXF_1	133	140	PF00149	0.306
DOC_PP4_FxxP_1	197	200	PF00568	0.306
DOC_USP7_MATH_1	162	166	PF00917	0.349
DOC_USP7_MATH_1	204	208	PF00917	0.426
DOC_USP7_MATH_1	45	49	PF00917	0.608
DOC_USP7_MATH_2	20	26	PF00917	0.647
DOC_WW_Pin1_4	16	21	PF00397	0.614
DOC_WW_Pin1_4	196	201	PF00397	0.303
DOC_WW_Pin1_4	343	348	PF00397	0.397
DOC_WW_Pin1_4	361	366	PF00397	0.420
LIG_14-3-3_CanoR_1	148	157	PF00244	0.328
LIG_14-3-3_CanoR_1	191	195	PF00244	0.363
LIG_Actin_WH2_2	70	85	PF00022	0.363
LIG_BRCT_BRCA1_1	198	202	PF00533	0.322
LIG_DCNL_PONY_1	1	4	PF03556	0.694
LIG_eIF4E_1	3	9	PF01652	0.609
LIG_FHA_1	138	144	PF00498	0.363
LIG_FHA_1	152	158	PF00498	0.375
LIG_FHA_1	223	229	PF00498	0.492
LIG_FHA_1	234	240	PF00498	0.366
LIG_FHA_1	288	294	PF00498	0.283
LIG_FHA_2	274	280	PF00498	0.414
LIG_FHA_2	87	93	PF00498	0.367
LIG_LIR_Gen_1	73	82	PF02991	0.375
LIG_LIR_Gen_1	91	100	PF02991	0.323
LIG_LIR_LC3C_4	290	295	PF02991	0.429
LIG_LIR_Nem_3	103	107	PF02991	0.293
LIG_LIR_Nem_3	199	205	PF02991	0.322
LIG_LIR_Nem_3	91	96	PF02991	0.293
LIG_MAD2	237	245	PF02301	0.426
LIG_Rb_LxCxE_1	58	75	PF01857	0.467
LIG_Rb_pABgroove_1	170	178	PF01858	0.341
LIG_REV1ctd_RIR_1	249	257	PF16727	0.375
LIG_SH2_CRK	303	307	PF00017	0.426
LIG_SH2_NCK_1	258	262	PF00017	0.457
LIG_SH2_STAT5	150	153	PF00017	0.324
LIG_SH2_STAT5	258	261	PF00017	0.337
LIG_SH2_STAT5	3	6	PF00017	0.607
LIG_SH2_STAT5	339	342	PF00017	0.322
LIG_SH2_STAT5	353	356	PF00017	0.404
LIG_SH3_3	197	203	PF00018	0.322
LIG_SH3_3	242	248	PF00018	0.392
LIG_SH3_3	359	365	PF00018	0.477
LIG_SH3_3	66	72	PF00018	0.492
LIG_SUMO_SIM_anti_2	292	299	PF11976	0.417
LIG_SUMO_SIM_par_1	235	242	PF11976	0.409
LIG_SUMO_SIM_par_1	292	299	PF11976	0.344
LIG_SUMO_SIM_par_1	304	310	PF11976	0.333
LIG_SxIP_EBH_1	137	148	PF03271	0.375
LIG_TRAF2_1	19	22	PF00917	0.641
LIG_UBA3_1	129	135	PF00899	0.306
LIG_UBA3_1	284	289	PF00899	0.375
LIG_WRC_WIRS_1	71	76	PF05994	0.375
MOD_CDK_SPxxK_3	361	368	PF00069	0.617
MOD_CK1_1	190	196	PF00069	0.394
MOD_CK1_1	36	42	PF00069	0.494
MOD_CK1_1	363	369	PF00069	0.680
MOD_CK2_1	16	22	PF00069	0.640
MOD_CK2_1	306	312	PF00069	0.395
MOD_Cter_Amidation	9	12	PF01082	0.599
MOD_GlcNHglycan	113	116	PF01048	0.426
MOD_GlcNHglycan	14	17	PF01048	0.687
MOD_GlcNHglycan	21	27	PF01048	0.576
MOD_GlcNHglycan	259	262	PF01048	0.396
MOD_GlcNHglycan	270	273	PF01048	0.366
MOD_GlcNHglycan	323	326	PF01048	0.326
MOD_GlcNHglycan	365	368	PF01048	0.705
MOD_GlcNHglycan	5	8	PF01048	0.699
MOD_GlcNHglycan	84	87	PF01048	0.283
MOD_GSK3_1	12	19	PF00069	0.688
MOD_GSK3_1	120	127	PF00069	0.342
MOD_GSK3_1	183	190	PF00069	0.282
MOD_GSK3_1	233	240	PF00069	0.421
MOD_GSK3_1	82	89	PF00069	0.369
MOD_N-GLC_1	183	188	PF02516	0.257
MOD_NEK2_1	119	124	PF00069	0.322
MOD_NEK2_1	139	144	PF00069	0.123
MOD_NEK2_1	2	7	PF00069	0.659
MOD_NEK2_2	183	188	PF00069	0.276
MOD_NEK2_2	204	209	PF00069	0.341
MOD_PIKK_1	164	170	PF00454	0.341
MOD_PKA_2	190	196	PF00069	0.392
MOD_Plk_1	183	189	PF00069	0.247
MOD_Plk_4	124	130	PF00069	0.307
MOD_Plk_4	139	145	PF00069	0.351
MOD_ProDKin_1	16	22	PF00069	0.613
MOD_ProDKin_1	196	202	PF00069	0.303
MOD_ProDKin_1	343	349	PF00069	0.397
MOD_ProDKin_1	361	367	PF00069	0.436
MOD_SUMO_for_1	100	103	PF00179	0.453
TRG_DiLeu_BaEn_1	95	100	PF01217	0.426
TRG_DiLeu_BaLyEn_6	280	285	PF01217	0.414
TRG_ENDOCYTIC_2	107	110	PF00928	0.322
TRG_ENDOCYTIC_2	150	153	PF00928	0.341
TRG_ENDOCYTIC_2	303	306	PF00928	0.306
TRG_ENDOCYTIC_2	353	356	PF00928	0.419
TRG_ER_diArg_1	227	230	PF00400	0.395
TRG_ER_diArg_1	52	54	PF00400	0.589
TRG_PTS1	366	369	PF00515	0.617

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HVX8	Leptomonas seymouri	31%	94%
A0A0N1PEN4	Leptomonas seymouri	73%	98%
A0A0S4J4T5	Bodo saltans	48%	91%
A0A0S4J8X9	Bodo saltans	45%	100%
A0A0S4JTX6	Bodo saltans	36%	91%
A0A1X0P1U7	Trypanosomatidae	51%	99%
A0A3Q8IA09	Leishmania donovani	30%	100%
A0A3R7KPG5	Trypanosoma rangeli	50%	100%
A0A422N3G9	Trypanosoma rangeli	30%	82%
A1RX40	Thermofilum pendens (strain DSM 2475 / Hrk 5)	26%	82%
A4FWR5	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	26%	88%
A4H811	Leishmania braziliensis	28%	100%
A4HPW0	Leishmania braziliensis	85%	100%
A4HWE1	Leishmania infantum	30%	100%
A4IDM2	Leishmania infantum	100%	100%
A4YHH3	Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2)	25%	84%
A5UK11	Methanobrevibacter smithii (strain ATCC 35061 / DSM 861 / OCM 144 / PS)	27%	85%
A6UPR4	Methanococcus vannielii (strain ATCC 35089 / DSM 1224 / JCM 13029 / OCM 148 / SB)	25%	87%
A6VGK5	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	27%	88%
A9AA46	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	26%	88%
B0R6H4	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	26%	86%
C3MPS1	Sulfolobus islandicus (strain L.S.2.15 / Lassen #1)	25%	83%
C3MYR5	Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)	25%	83%
C3N5E7	Sulfolobus islandicus (strain M.16.27)	25%	83%
C3NE01	Sulfolobus islandicus (strain Y.G.57.14 / Yellowstone #1)	25%	83%
C3NHQ2	Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)	25%	83%
C4KH13	Sulfolobus islandicus (strain M.16.4 / Kamchatka #3)	25%	83%
E9AQ41	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9ATM7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
O26802	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	26%	85%
O29380	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	27%	88%
O59132	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	28%	84%
P0A962	Escherichia coli (strain K12)	37%	100%
P0A963	Escherichia coli O157:H7	37%	100%
P26900	Bacillus subtilis (strain 168)	32%	100%
P61400	Methanococcus maripaludis (strain S2 / LL)	26%	88%
P61401	Nanoarchaeum equitans (strain Kin4-M)	27%	94%
Q12X65	Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / ACE-M)	25%	89%
Q2NEH1	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	24%	84%
Q46GJ6	Methanosarcina barkeri (strain Fusaro / DSM 804)	26%	87%
Q4J955	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	22%	83%
Q4Q0X1	Leishmania major	95%	100%
Q4QFE1	Leishmania major	29%	100%
Q5JI77	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	28%	84%
Q60331	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	27%	88%
Q8PUM7	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	25%	87%
Q8TR66	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	24%	87%
Q8TV84	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	25%	81%
Q8TZE8	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	32%	100%
Q8U0X0	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	27%	84%
Q8ZY04	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	24%	88%
Q971W5	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	26%	82%
Q979L8	Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1)	29%	91%
Q97ZH5	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	25%	83%
Q9HJJ5	Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)	28%	90%
Q9HP20	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	26%	86%
Q9RRX9	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	26%	100%
Q9V0T9	Pyrococcus abyssi (strain GE5 / Orsay)	28%	84%
Q9Y9T8	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	31%	86%
V5BUU3	Trypanosoma cruzi	50%	100%