NmrA-like_family/NADH(P)-binding/NAD_dependent_ep imerase/dehydratase_family/3-beta_hydroxysteroid_dehydrogen ase/isomerase_family_putative/Pfam:PF05368/Pfam:PF13460/Pfa m:PF01370/Pfam:PF01073

Quick info Annotations Localization Phosphorylation Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NAD dependent epimerase/dehydratase family, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X885_LEIDO

TriTrypDb:

LdBPK_342970.1 , LdCL_340038500 , LDHU3_34.5090

Length:

510

Annotations

Annotations by Jardim et al.

Oxidoreductase, NAD dependent epimerase/dehydratase family Uncharacterized

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10
GO:0005739	mitochondrion	5	1
GO:0005741	mitochondrial outer membrane	5	1
GO:0019867	outer membrane	3	1
GO:0031090	organelle membrane	3	1
GO:0031966	mitochondrial membrane	4	1
GO:0031968	organelle outer membrane	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0098588	bounding membrane of organelle	4	1

Phosphorylation

Promastigote: 60, 72

Expansion

Sequence features

A0A3S7X885

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X885

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0016853	isomerase activity	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	143	147	PF00656	0.274
CLV_C14_Caspase3-7	179	183	PF00656	0.268
CLV_C14_Caspase3-7	340	344	PF00656	0.252
CLV_NRD_NRD_1	417	419	PF00675	0.270
CLV_NRD_NRD_1	424	426	PF00675	0.277
CLV_NRD_NRD_1	472	474	PF00675	0.536
CLV_NRD_NRD_1	508	510	PF00675	0.685
CLV_PCSK_KEX2_1	229	231	PF00082	0.407
CLV_PCSK_KEX2_1	419	421	PF00082	0.304
CLV_PCSK_KEX2_1	424	426	PF00082	0.313
CLV_PCSK_KEX2_1	472	474	PF00082	0.536
CLV_PCSK_KEX2_1	508	510	PF00082	0.689
CLV_PCSK_PC1ET2_1	229	231	PF00082	0.415
CLV_PCSK_PC1ET2_1	419	421	PF00082	0.320
CLV_PCSK_PC1ET2_1	472	474	PF00082	0.525
CLV_PCSK_PC1ET2_1	508	510	PF00082	0.732
CLV_PCSK_PC7_1	420	426	PF00082	0.380
CLV_PCSK_SKI1_1	108	112	PF00082	0.362
CLV_PCSK_SKI1_1	214	218	PF00082	0.446
CLV_PCSK_SKI1_1	230	234	PF00082	0.449
CLV_PCSK_SKI1_1	273	277	PF00082	0.470
CLV_PCSK_SKI1_1	373	377	PF00082	0.551
CLV_PCSK_SKI1_1	424	428	PF00082	0.320
CLV_PCSK_SKI1_1	450	454	PF00082	0.363
CLV_PCSK_SKI1_1	505	509	PF00082	0.661
CLV_Separin_Metazoa	42	46	PF03568	0.659
DEG_APCC_DBOX_1	441	449	PF00400	0.525
DEG_Nend_Nbox_1	1	3	PF02207	0.601
DOC_CKS1_1	261	266	PF01111	0.252
DOC_CKS1_1	381	386	PF01111	0.382
DOC_CYCLIN_RxL_1	211	218	PF00134	0.262
DOC_CYCLIN_yCln2_LP_2	484	490	PF00134	0.239
DOC_MAPK_gen_1	106	114	PF00069	0.525
DOC_MAPK_gen_1	282	291	PF00069	0.206
DOC_MAPK_gen_1	424	434	PF00069	0.517
DOC_MAPK_MEF2A_6	108	116	PF00069	0.572
DOC_MAPK_MEF2A_6	282	291	PF00069	0.256
DOC_MAPK_MEF2A_6	425	434	PF00069	0.500
DOC_MAPK_MEF2A_6	483	491	PF00069	0.495
DOC_PP1_RVXF_1	271	277	PF00149	0.215
DOC_PP2B_LxvP_1	382	385	PF13499	0.270
DOC_PP2B_PxIxI_1	295	301	PF00149	0.215
DOC_USP7_MATH_1	236	240	PF00917	0.266
DOC_USP7_MATH_1	319	323	PF00917	0.246
DOC_USP7_MATH_1	477	481	PF00917	0.721
DOC_USP7_MATH_1	69	73	PF00917	0.650
DOC_USP7_UBL2_3	225	229	PF12436	0.228
DOC_WW_Pin1_4	196	201	PF00397	0.318
DOC_WW_Pin1_4	260	265	PF00397	0.366
DOC_WW_Pin1_4	380	385	PF00397	0.306
DOC_WW_Pin1_4	432	437	PF00397	0.488
DOC_WW_Pin1_4	483	488	PF00397	0.529
DOC_WW_Pin1_4	77	82	PF00397	0.703
LIG_14-3-3_CanoR_1	141	145	PF00244	0.226
LIG_Actin_WH2_2	167	183	PF00022	0.226
LIG_BRCT_BRCA1_1	249	253	PF00533	0.241
LIG_BRCT_BRCA1_1	321	325	PF00533	0.226
LIG_BRCT_BRCA1_1	331	335	PF00533	0.226
LIG_EH1_1	127	135	PF00400	0.268
LIG_FHA_1	208	214	PF00498	0.251
LIG_FHA_1	392	398	PF00498	0.244
LIG_FHA_1	77	83	PF00498	0.717
LIG_FHA_2	141	147	PF00498	0.226
LIG_FHA_2	242	248	PF00498	0.239
LIG_FHA_2	261	267	PF00498	0.252
LIG_FHA_2	438	444	PF00498	0.563
LIG_FHA_2	98	104	PF00498	0.636
LIG_LIR_Gen_1	255	264	PF02991	0.239
LIG_LIR_Gen_1	332	342	PF02991	0.284
LIG_LIR_Gen_1	388	398	PF02991	0.264
LIG_LIR_Gen_1	52	61	PF02991	0.715
LIG_LIR_LC3C_4	290	293	PF02991	0.226
LIG_LIR_Nem_3	255	261	PF02991	0.226
LIG_LIR_Nem_3	321	327	PF02991	0.290
LIG_LIR_Nem_3	332	338	PF02991	0.220
LIG_LIR_Nem_3	388	393	PF02991	0.253
LIG_LIR_Nem_3	52	56	PF02991	0.716
LIG_MLH1_MIPbox_1	321	325	PF16413	0.316
LIG_MYND_1	380	384	PF01753	0.293
LIG_Pex14_2	53	57	PF04695	0.720
LIG_Rb_pABgroove_1	211	219	PF01858	0.226
LIG_SH2_SRC	189	192	PF00017	0.199
LIG_SH2_STAP1	138	142	PF00017	0.227
LIG_SH2_STAP1	209	213	PF00017	0.316
LIG_SH2_STAT5	124	127	PF00017	0.263
LIG_SH2_STAT5	189	192	PF00017	0.215
LIG_SH2_STAT5	209	212	PF00017	0.316
LIG_SH2_STAT5	324	327	PF00017	0.289
LIG_SH2_STAT5	506	509	PF00017	0.508
LIG_SH3_1	323	329	PF00018	0.296
LIG_SH3_3	21	27	PF00018	0.604
LIG_SH3_3	258	264	PF00018	0.342
LIG_SH3_3	290	296	PF00018	0.206
LIG_SH3_3	323	329	PF00018	0.227
LIG_SH3_3	363	369	PF00018	0.348
LIG_SH3_3	40	46	PF00018	0.503
LIG_SH3_3	85	91	PF00018	0.610
LIG_SUMO_SIM_anti_2	290	297	PF11976	0.215
LIG_SUMO_SIM_par_1	115	122	PF11976	0.395
LIG_SUMO_SIM_par_1	204	210	PF11976	0.304
LIG_SUMO_SIM_par_1	290	297	PF11976	0.209
LIG_SUMO_SIM_par_1	306	316	PF11976	0.294
LIG_SUMO_SIM_par_1	489	495	PF11976	0.376
LIG_UBA3_1	216	225	PF00899	0.268
MOD_CDK_SPxxK_3	196	203	PF00069	0.398
MOD_CK1_1	196	202	PF00069	0.395
MOD_CK1_1	223	229	PF00069	0.415
MOD_CK1_1	260	266	PF00069	0.417
MOD_CK1_1	492	498	PF00069	0.350
MOD_CK1_1	8	14	PF00069	0.513
MOD_CK2_1	364	370	PF00069	0.296
MOD_CK2_1	97	103	PF00069	0.447
MOD_Cter_Amidation	470	473	PF01082	0.553
MOD_GlcNHglycan	121	124	PF01048	0.355
MOD_GlcNHglycan	196	199	PF01048	0.371
MOD_GlcNHglycan	222	225	PF01048	0.303
MOD_GlcNHglycan	249	252	PF01048	0.247
MOD_GlcNHglycan	39	42	PF01048	0.641
MOD_GlcNHglycan	450	453	PF01048	0.504
MOD_GlcNHglycan	5	8	PF01048	0.538
MOD_GlcNHglycan	57	60	PF01048	0.410
MOD_GSK3_1	136	143	PF00069	0.256
MOD_GSK3_1	14	21	PF00069	0.617
MOD_GSK3_1	172	179	PF00069	0.292
MOD_GSK3_1	220	227	PF00069	0.361
MOD_GSK3_1	231	238	PF00069	0.294
MOD_GSK3_1	253	260	PF00069	0.269
MOD_GSK3_1	296	303	PF00069	0.285
MOD_N-GLC_1	319	324	PF02516	0.347
MOD_N-GLC_1	329	334	PF02516	0.347
MOD_N-GLC_2	363	365	PF02516	0.333
MOD_NEK2_1	253	258	PF00069	0.241
MOD_NEK2_1	318	323	PF00069	0.395
MOD_NEK2_1	402	407	PF00069	0.296
MOD_NEK2_1	411	416	PF00069	0.336
MOD_NEK2_1	430	435	PF00069	0.243
MOD_NEK2_1	437	442	PF00069	0.322
MOD_NEK2_1	448	453	PF00069	0.313
MOD_NEK2_1	5	10	PF00069	0.515
MOD_NEK2_1	57	62	PF00069	0.523
MOD_NEK2_2	136	141	PF00069	0.246
MOD_NEK2_2	224	229	PF00069	0.279
MOD_PKA_2	140	146	PF00069	0.253
MOD_PKA_2	165	171	PF00069	0.287
MOD_PKA_2	220	226	PF00069	0.292
MOD_PKA_2	465	471	PF00069	0.548
MOD_Plk_1	329	335	PF00069	0.376
MOD_Plk_2-3	364	370	PF00069	0.447
MOD_Plk_4	14	20	PF00069	0.663
MOD_Plk_4	165	171	PF00069	0.287
MOD_Plk_4	236	242	PF00069	0.268
MOD_Plk_4	396	402	PF00069	0.277
MOD_Plk_4	489	495	PF00069	0.324
MOD_Plk_4	90	96	PF00069	0.457
MOD_ProDKin_1	196	202	PF00069	0.398
MOD_ProDKin_1	260	266	PF00069	0.464
MOD_ProDKin_1	380	386	PF00069	0.377
MOD_ProDKin_1	432	438	PF00069	0.360
MOD_ProDKin_1	483	489	PF00069	0.413
MOD_ProDKin_1	77	83	PF00069	0.656
TRG_DiLeu_BaEn_1	25	30	PF01217	0.519
TRG_DiLeu_BaEn_1	290	295	PF01217	0.395
TRG_ENDOCYTIC_2	324	327	PF00928	0.376
TRG_ER_diArg_1	281	284	PF00400	0.386
TRG_ER_diArg_1	417	420	PF00400	0.387
TRG_Pf-PMV_PEXEL_1	214	218	PF00026	0.313

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P451	Leptomonas seymouri	82%	100%
A0A0S4ILP0	Bodo saltans	53%	100%
A0A0S4KPM4	Bodo saltans	30%	84%
A0A1X0PAG0	Trypanosomatidae	25%	100%
A0A1X0PAW0	Trypanosomatidae	53%	98%
A0A3S5IRZ5	Trypanosoma rangeli	24%	100%
A0A422NNP8	Trypanosoma rangeli	56%	100%
A4HB38	Leishmania braziliensis	91%	100%
A4IAN1	Leishmania infantum	100%	100%
C9ZM09	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
E9B5A8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
P53111	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	22%	100%
P53183	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
P83775	Candida albicans (strain SC5314 / ATCC MYA-2876)	22%	100%
Q12068	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
Q4Q2M5	Leishmania major	98%	100%
Q9S9N9	Arabidopsis thaliana	28%	100%
V5B687	Trypanosoma cruzi	26%	100%
V5DLY1	Trypanosoma cruzi	58%	100%