Asparagine--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

asparaginyl-tRNA synthetase, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X7X7_LEIDO

TriTrypDb:

LdBPK_342110.1 , LdCL_340028800 , LDHU3_34.3540

Length:

890

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

A0A3S7X7X7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X7X7

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006421	asparaginyl-tRNA aminoacylation	7	10
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0004816	asparagine-tRNA ligase activity	5	10
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	826	830	PF00656	0.536
CLV_C14_Caspase3-7	834	838	PF00656	0.484
CLV_C14_Caspase3-7	874	878	PF00656	0.517
CLV_MEL_PAP_1	692	698	PF00089	0.329
CLV_NRD_NRD_1	175	177	PF00675	0.767
CLV_NRD_NRD_1	380	382	PF00675	0.417
CLV_NRD_NRD_1	730	732	PF00675	0.302
CLV_NRD_NRD_1	848	850	PF00675	0.302
CLV_PCSK_FUR_1	173	177	PF00082	0.668
CLV_PCSK_KEX2_1	175	177	PF00082	0.767
CLV_PCSK_KEX2_1	380	382	PF00082	0.316
CLV_PCSK_KEX2_1	656	658	PF00082	0.292
CLV_PCSK_KEX2_1	726	728	PF00082	0.302
CLV_PCSK_KEX2_1	730	732	PF00082	0.283
CLV_PCSK_KEX2_1	832	834	PF00082	0.361
CLV_PCSK_KEX2_1	848	850	PF00082	0.283
CLV_PCSK_PC1ET2_1	656	658	PF00082	0.359
CLV_PCSK_PC1ET2_1	726	728	PF00082	0.394
CLV_PCSK_PC1ET2_1	832	834	PF00082	0.396
CLV_PCSK_PC7_1	652	658	PF00082	0.306
CLV_PCSK_SKI1_1	246	250	PF00082	0.316
CLV_PCSK_SKI1_1	279	283	PF00082	0.302
CLV_PCSK_SKI1_1	394	398	PF00082	0.429
CLV_PCSK_SKI1_1	92	96	PF00082	0.659
CLV_Separin_Metazoa	578	582	PF03568	0.568
DEG_APCC_DBOX_1	575	583	PF00400	0.510
DEG_SCF_FBW7_1	190	197	PF00400	0.612
DEG_SPOP_SBC_1	129	133	PF00917	0.602
DOC_CKS1_1	191	196	PF01111	0.616
DOC_CYCLIN_yCln2_LP_2	107	113	PF00134	0.529
DOC_CYCLIN_yCln2_LP_2	165	171	PF00134	0.649
DOC_CYCLIN_yCln2_LP_2	522	528	PF00134	0.502
DOC_CYCLIN_yCln2_LP_2	62	68	PF00134	0.587
DOC_MAPK_gen_1	254	264	PF00069	0.501
DOC_MAPK_gen_1	367	376	PF00069	0.596
DOC_MAPK_gen_1	470	479	PF00069	0.492
DOC_MAPK_gen_1	574	582	PF00069	0.506
DOC_MAPK_gen_1	730	740	PF00069	0.492
DOC_MAPK_RevD_3	163	176	PF00069	0.637
DOC_PP1_RVXF_1	862	869	PF00149	0.492
DOC_PP2B_LxvP_1	107	110	PF13499	0.783
DOC_PP2B_LxvP_1	165	168	PF13499	0.716
DOC_PP2B_LxvP_1	522	525	PF13499	0.502
DOC_USP7_MATH_1	112	116	PF00917	0.584
DOC_USP7_MATH_1	129	133	PF00917	0.544
DOC_USP7_MATH_1	139	143	PF00917	0.753
DOC_USP7_MATH_1	210	214	PF00917	0.801
DOC_USP7_MATH_1	266	270	PF00917	0.456
DOC_USP7_MATH_1	322	326	PF00917	0.617
DOC_USP7_MATH_1	549	553	PF00917	0.545
DOC_USP7_MATH_1	650	654	PF00917	0.514
DOC_USP7_UBL2_3	722	726	PF12436	0.500
DOC_WW_Pin1_4	183	188	PF00397	0.692
DOC_WW_Pin1_4	190	195	PF00397	0.683
DOC_WW_Pin1_4	271	276	PF00397	0.496
DOC_WW_Pin1_4	445	450	PF00397	0.605
DOC_WW_Pin1_4	593	598	PF00397	0.502
DOC_WW_Pin1_4	61	66	PF00397	0.585
DOC_WW_Pin1_4	721	726	PF00397	0.609
DOC_WW_Pin1_4	82	87	PF00397	0.792
LIG_14-3-3_CanoR_1	148	157	PF00244	0.705
LIG_14-3-3_CanoR_1	173	179	PF00244	0.710
LIG_14-3-3_CanoR_1	18	23	PF00244	0.644
LIG_14-3-3_CanoR_1	476	480	PF00244	0.528
LIG_14-3-3_CanoR_1	657	665	PF00244	0.494
LIG_14-3-3_CanoR_1	92	101	PF00244	0.763
LIG_Actin_WH2_2	372	388	PF00022	0.559
LIG_APCC_ABBA_1	580	585	PF00400	0.506
LIG_BRCT_BRCA1_1	608	612	PF00533	0.492
LIG_eIF4E_1	822	828	PF01652	0.596
LIG_FHA_1	195	201	PF00498	0.607
LIG_FHA_1	229	235	PF00498	0.492
LIG_FHA_1	297	303	PF00498	0.618
LIG_FHA_1	308	314	PF00498	0.455
LIG_FHA_1	45	51	PF00498	0.824
LIG_FHA_1	484	490	PF00498	0.605
LIG_FHA_1	633	639	PF00498	0.512
LIG_FHA_1	799	805	PF00498	0.492
LIG_FHA_2	33	39	PF00498	0.621
LIG_FHA_2	364	370	PF00498	0.623
LIG_FHA_2	601	607	PF00498	0.499
LIG_FHA_2	608	614	PF00498	0.483
LIG_FHA_2	661	667	PF00498	0.502
LIG_FHA_2	689	695	PF00498	0.616
LIG_FHA_2	797	803	PF00498	0.516
LIG_GBD_Chelix_1	198	206	PF00786	0.561
LIG_LIR_Gen_1	478	484	PF02991	0.499
LIG_LIR_Gen_1	532	540	PF02991	0.557
LIG_LIR_Gen_1	609	617	PF02991	0.492
LIG_LIR_Gen_1	660	669	PF02991	0.536
LIG_LIR_Gen_1	673	681	PF02991	0.492
LIG_LIR_Gen_1	682	693	PF02991	0.485
LIG_LIR_Gen_1	775	784	PF02991	0.596
LIG_LIR_Nem_3	436	441	PF02991	0.456
LIG_LIR_Nem_3	478	482	PF02991	0.520
LIG_LIR_Nem_3	532	536	PF02991	0.547
LIG_LIR_Nem_3	589	595	PF02991	0.652
LIG_LIR_Nem_3	609	615	PF02991	0.534
LIG_LIR_Nem_3	660	665	PF02991	0.536
LIG_LIR_Nem_3	673	677	PF02991	0.492
LIG_LIR_Nem_3	682	688	PF02991	0.491
LIG_LIR_Nem_3	839	844	PF02991	0.511
LIG_MYND_1	86	90	PF01753	0.613
LIG_Pex14_1	588	592	PF04695	0.596
LIG_Pex14_2	583	587	PF04695	0.502
LIG_Pex14_2	778	782	PF04695	0.534
LIG_Rb_pABgroove_1	551	559	PF01858	0.471
LIG_RPA_C_Fungi	340	352	PF08784	0.346
LIG_SH2_CRK	533	537	PF00017	0.408
LIG_SH2_NCK_1	533	537	PF00017	0.408
LIG_SH2_SRC	630	633	PF00017	0.453
LIG_SH2_SRC	734	737	PF00017	0.384
LIG_SH2_STAP1	734	738	PF00017	0.360
LIG_SH2_STAP1	824	828	PF00017	0.345
LIG_SH2_STAT3	622	625	PF00017	0.397
LIG_SH2_STAT3	844	847	PF00017	0.326
LIG_SH2_STAT5	463	466	PF00017	0.419
LIG_SH2_STAT5	622	625	PF00017	0.357
LIG_SH2_STAT5	630	633	PF00017	0.324
LIG_SH2_STAT5	642	645	PF00017	0.206
LIG_SH2_STAT5	844	847	PF00017	0.326
LIG_SH3_1	722	728	PF00018	0.333
LIG_SH3_2	725	730	PF14604	0.326
LIG_SH3_2	87	92	PF14604	0.663
LIG_SH3_3	106	112	PF00018	0.540
LIG_SH3_3	184	190	PF00018	0.753
LIG_SH3_3	286	292	PF00018	0.424
LIG_SH3_3	443	449	PF00018	0.568
LIG_SH3_3	592	598	PF00018	0.326
LIG_SH3_3	712	718	PF00018	0.345
LIG_SH3_3	722	728	PF00018	0.413
LIG_SH3_3	792	798	PF00018	0.397
LIG_SH3_3	803	809	PF00018	0.341
LIG_SH3_3	84	90	PF00018	0.663
LIG_SH3_3	98	104	PF00018	0.509
LIG_SUMO_SIM_anti_2	801	808	PF11976	0.326
LIG_SUMO_SIM_par_1	103	108	PF11976	0.603
LIG_TRAF2_2	705	710	PF00917	0.397
LIG_UBA3_1	611	620	PF00899	0.294
LIG_UBA3_1	869	876	PF00899	0.471
LIG_WRC_WIRS_1	671	676	PF05994	0.471
LIG_WRC_WIRS_1	786	791	PF05994	0.326
LIG_WW_2	109	112	PF00397	0.517
MOD_CDK_SPK_2	183	188	PF00069	0.770
MOD_CDK_SPK_2	271	276	PF00069	0.326
MOD_CDK_SPK_2	721	726	PF00069	0.346
MOD_CDK_SPxK_1	190	196	PF00069	0.719
MOD_CDK_SPxK_1	721	727	PF00069	0.471
MOD_CK1_1	130	136	PF00069	0.654
MOD_CK1_1	142	148	PF00069	0.741
MOD_CK1_1	152	158	PF00069	0.678
MOD_CK1_1	220	226	PF00069	0.736
MOD_CK1_1	475	481	PF00069	0.341
MOD_CK1_1	539	545	PF00069	0.496
MOD_CK1_1	660	666	PF00069	0.335
MOD_CK1_1	785	791	PF00069	0.346
MOD_CK1_1	80	86	PF00069	0.766
MOD_CK2_1	250	256	PF00069	0.320
MOD_CK2_1	266	272	PF00069	0.320
MOD_CK2_1	32	38	PF00069	0.619
MOD_CK2_1	452	458	PF00069	0.490
MOD_CK2_1	600	606	PF00069	0.360
MOD_CK2_1	607	613	PF00069	0.360
MOD_CK2_1	660	666	PF00069	0.326
MOD_CK2_1	688	694	PF00069	0.397
MOD_CK2_1	796	802	PF00069	0.377
MOD_GlcNHglycan	132	135	PF01048	0.735
MOD_GlcNHglycan	210	213	PF01048	0.808
MOD_GSK3_1	124	131	PF00069	0.809
MOD_GSK3_1	135	142	PF00069	0.774
MOD_GSK3_1	149	156	PF00069	0.676
MOD_GSK3_1	190	197	PF00069	0.687
MOD_GSK3_1	545	552	PF00069	0.480
MOD_GSK3_1	564	571	PF00069	0.500
MOD_GSK3_1	596	603	PF00069	0.404
MOD_GSK3_1	630	637	PF00069	0.430
MOD_GSK3_1	684	691	PF00069	0.436
MOD_GSK3_1	77	84	PF00069	0.829
MOD_GSK3_1	796	803	PF00069	0.373
MOD_NEK2_1	135	140	PF00069	0.822
MOD_NEK2_1	208	213	PF00069	0.716
MOD_NEK2_1	307	312	PF00069	0.341
MOD_NEK2_1	568	573	PF00069	0.413
MOD_NEK2_1	600	605	PF00069	0.374
MOD_NEK2_1	623	628	PF00069	0.357
MOD_NEK2_1	66	71	PF00069	0.737
MOD_NEK2_1	684	689	PF00069	0.398
MOD_NEK2_1	81	86	PF00069	0.626
MOD_NEK2_1	815	820	PF00069	0.346
MOD_NEK2_1	836	841	PF00069	0.388
MOD_NEK2_1	97	102	PF00069	0.705
MOD_NEK2_2	607	612	PF00069	0.326
MOD_PIKK_1	307	313	PF00454	0.326
MOD_PIKK_1	600	606	PF00454	0.375
MOD_PIKK_1	621	627	PF00454	0.362
MOD_PIKK_1	703	709	PF00454	0.388
MOD_PK_1	18	24	PF00069	0.552
MOD_PKA_2	174	180	PF00069	0.614
MOD_PKA_2	475	481	PF00069	0.341
MOD_PKA_2	501	507	PF00069	0.326
MOD_PKA_2	508	514	PF00069	0.326
MOD_PKA_2	564	570	PF00069	0.327
MOD_PKB_1	16	24	PF00069	0.756
MOD_PKB_1	470	478	PF00069	0.332
MOD_Plk_1	220	226	PF00069	0.644
MOD_Plk_1	549	555	PF00069	0.580
MOD_Plk_1	660	666	PF00069	0.370
MOD_Plk_1	836	842	PF00069	0.451
MOD_Plk_2-3	670	676	PF00069	0.433
MOD_Plk_2-3	796	802	PF00069	0.362
MOD_Plk_4	201	207	PF00069	0.710
MOD_Plk_4	475	481	PF00069	0.419
MOD_Plk_4	542	548	PF00069	0.595
MOD_Plk_4	568	574	PF00069	0.419
MOD_Plk_4	596	602	PF00069	0.348
MOD_Plk_4	607	613	PF00069	0.326
MOD_Plk_4	684	690	PF00069	0.405
MOD_Plk_4	785	791	PF00069	0.355
MOD_Plk_4	837	843	PF00069	0.365
MOD_Plk_4	97	103	PF00069	0.796
MOD_ProDKin_1	183	189	PF00069	0.691
MOD_ProDKin_1	190	196	PF00069	0.685
MOD_ProDKin_1	271	277	PF00069	0.332
MOD_ProDKin_1	445	451	PF00069	0.608
MOD_ProDKin_1	593	599	PF00069	0.341
MOD_ProDKin_1	61	67	PF00069	0.586
MOD_ProDKin_1	721	727	PF00069	0.489
MOD_ProDKin_1	82	88	PF00069	0.795
MOD_SUMO_for_1	417	420	PF00179	0.723
MOD_SUMO_for_1	425	428	PF00179	0.735
MOD_SUMO_for_1	518	521	PF00179	0.371
MOD_SUMO_rev_2	271	281	PF00179	0.333
MOD_SUMO_rev_2	407	415	PF00179	0.628
TRG_DiLeu_BaLyEn_6	861	866	PF01217	0.384
TRG_ENDOCYTIC_2	533	536	PF00928	0.408
TRG_ENDOCYTIC_2	592	595	PF00928	0.471
TRG_ENDOCYTIC_2	642	645	PF00928	0.326
TRG_ENDOCYTIC_2	824	827	PF00928	0.345
TRG_ER_diArg_1	16	19	PF00400	0.620
TRG_ER_diArg_1	172	175	PF00400	0.763
TRG_ER_diArg_1	379	381	PF00400	0.471
TRG_ER_diArg_1	729	731	PF00400	0.327
TRG_ER_diArg_1	8	11	PF00400	0.700
TRG_ER_diArg_1	848	850	PF00400	0.462
TRG_Pf-PMV_PEXEL_1	657	661	PF00026	0.326

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMR3	Leptomonas seymouri	77%	100%
A0A0S4JDH2	Bodo saltans	60%	100%
A0A1X0P9Y4	Trypanosomatidae	62%	100%
A0A3R7P1H1	Trypanosoma rangeli	63%	100%
A4HAU6	Leishmania braziliensis	76%	100%
A4IA13	Leishmania infantum	100%	100%
C9ZMM2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	100%
E9AV72	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9B520	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	99%
Q4Q2W6	Leishmania major	94%	100%
Q554D9	Dictyostelium discoideum	39%	100%
V5DGS5	Trypanosoma cruzi	63%	100%