ThiF domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania donovani

UniProt:

A0A3S7X724_LEIDO

TriTrypDb:

LdBPK_332790.1 , LdCL_330035000 , LDHU3_33.3930

Length:

799

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S7X724

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X724

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0045116	protein neddylation	7	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	8
GO:0016874	ligase activity	2	8
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	8
GO:0140096	catalytic activity, acting on a protein	2	8
GO:0140657	ATP-dependent activity	1	8
GO:0019781	NEDD8 activating enzyme activity	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	130	134	PF00656	0.336
CLV_NRD_NRD_1	323	325	PF00675	0.450
CLV_NRD_NRD_1	365	367	PF00675	0.460
CLV_NRD_NRD_1	571	573	PF00675	0.543
CLV_NRD_NRD_1	655	657	PF00675	0.423
CLV_NRD_NRD_1	672	674	PF00675	0.489
CLV_NRD_NRD_1	730	732	PF00675	0.387
CLV_PCSK_KEX2_1	323	325	PF00082	0.450
CLV_PCSK_KEX2_1	334	336	PF00082	0.428
CLV_PCSK_KEX2_1	365	367	PF00082	0.460
CLV_PCSK_KEX2_1	655	657	PF00082	0.423
CLV_PCSK_KEX2_1	672	674	PF00082	0.489
CLV_PCSK_PC1ET2_1	334	336	PF00082	0.468
CLV_PCSK_SKI1_1	168	172	PF00082	0.481
CLV_PCSK_SKI1_1	21	25	PF00082	0.471
CLV_PCSK_SKI1_1	595	599	PF00082	0.443
CLV_PCSK_SKI1_1	67	71	PF00082	0.402
CLV_PCSK_SKI1_1	731	735	PF00082	0.422
DEG_MDM2_SWIB_1	322	329	PF02201	0.444
DOC_CYCLIN_RxL_1	592	602	PF00134	0.432
DOC_MAPK_DCC_7	232	242	PF00069	0.424
DOC_MAPK_gen_1	21	31	PF00069	0.349
DOC_MAPK_MEF2A_6	232	241	PF00069	0.407
DOC_MAPK_MEF2A_6	774	781	PF00069	0.425
DOC_PP1_RVXF_1	19	26	PF00149	0.450
DOC_PP1_RVXF_1	611	618	PF00149	0.424
DOC_PP1_RVXF_1	634	640	PF00149	0.432
DOC_PP2B_LxvP_1	204	207	PF13499	0.373
DOC_USP7_MATH_1	190	194	PF00917	0.644
DOC_USP7_MATH_1	299	303	PF00917	0.508
DOC_USP7_MATH_1	355	359	PF00917	0.429
DOC_USP7_MATH_1	424	428	PF00917	0.500
DOC_USP7_MATH_1	57	61	PF00917	0.376
DOC_USP7_MATH_1	677	681	PF00917	0.548
DOC_USP7_UBL2_3	380	384	PF12436	0.482
DOC_USP7_UBL2_3	482	486	PF12436	0.781
DOC_WW_Pin1_4	194	199	PF00397	0.642
DOC_WW_Pin1_4	217	222	PF00397	0.426
DOC_WW_Pin1_4	418	423	PF00397	0.500
DOC_WW_Pin1_4	426	431	PF00397	0.445
DOC_WW_Pin1_4	440	445	PF00397	0.311
DOC_WW_Pin1_4	92	97	PF00397	0.353
LIG_14-3-3_CanoR_1	324	330	PF00244	0.480
LIG_14-3-3_CanoR_1	335	345	PF00244	0.616
LIG_14-3-3_CanoR_1	354	360	PF00244	0.448
LIG_14-3-3_CanoR_1	411	417	PF00244	0.341
LIG_14-3-3_CanoR_1	586	590	PF00244	0.441
LIG_14-3-3_CanoR_1	613	618	PF00244	0.448
LIG_14-3-3_CanoR_1	642	647	PF00244	0.523
LIG_14-3-3_CanoR_1	67	72	PF00244	0.402
LIG_Actin_WH2_2	386	404	PF00022	0.460
LIG_BIR_II_1	1	5	PF00653	0.678
LIG_BRCT_BRCA1_1	342	346	PF00533	0.588
LIG_BRCT_BRCA1_1	83	87	PF00533	0.273
LIG_FHA_1	161	167	PF00498	0.672
LIG_FHA_1	380	386	PF00498	0.421
LIG_FHA_1	458	464	PF00498	0.466
LIG_FHA_1	49	55	PF00498	0.350
LIG_FHA_1	525	531	PF00498	0.495
LIG_FHA_1	585	591	PF00498	0.445
LIG_FHA_1	600	606	PF00498	0.485
LIG_FHA_1	639	645	PF00498	0.519
LIG_FHA_1	66	72	PF00498	0.360
LIG_FHA_1	762	768	PF00498	0.419
LIG_FHA_1	791	797	PF00498	0.469
LIG_FHA_2	197	203	PF00498	0.550
LIG_FHA_2	326	332	PF00498	0.455
LIG_FHA_2	68	74	PF00498	0.360
LIG_LIR_Gen_1	171	179	PF02991	0.551
LIG_LIR_Nem_3	171	176	PF02991	0.584
LIG_LIR_Nem_3	213	219	PF02991	0.384
LIG_LIR_Nem_3	278	283	PF02991	0.437
LIG_LIR_Nem_3	310	314	PF02991	0.387
LIG_LIR_Nem_3	352	356	PF02991	0.366
LIG_LIR_Nem_3	529	535	PF02991	0.377
LIG_LYPXL_yS_3	314	317	PF13949	0.378
LIG_MLH1_MIPbox_1	83	87	PF16413	0.273
LIG_NRBOX	396	402	PF00104	0.378
LIG_PDZ_Class_2	794	799	PF00595	0.441
LIG_Pex14_1	124	128	PF04695	0.425
LIG_Pex14_1	136	140	PF04695	0.476
LIG_Pex14_2	322	326	PF04695	0.463
LIG_PTB_Apo_2	791	798	PF02174	0.440
LIG_SH2_CRK	173	177	PF00017	0.485
LIG_SH2_CRK	353	357	PF00017	0.425
LIG_SH2_CRK	514	518	PF00017	0.458
LIG_SH2_GRB2like	792	795	PF00017	0.450
LIG_SH2_NCK_1	173	177	PF00017	0.400
LIG_SH2_SRC	140	143	PF00017	0.642
LIG_SH2_STAP1	173	177	PF00017	0.400
LIG_SH2_STAP1	601	605	PF00017	0.303
LIG_SH2_STAP1	792	796	PF00017	0.448
LIG_SH2_STAT5	140	143	PF00017	0.449
LIG_SH2_STAT5	254	257	PF00017	0.420
LIG_SH2_STAT5	311	314	PF00017	0.355
LIG_SH2_STAT5	521	524	PF00017	0.442
LIG_SH2_STAT5	601	604	PF00017	0.276
LIG_SH2_STAT5	664	667	PF00017	0.377
LIG_SH2_STAT5	792	795	PF00017	0.450
LIG_SH2_STAT5	86	89	PF00017	0.376
LIG_SH3_1	153	159	PF00018	0.558
LIG_SH3_1	232	238	PF00018	0.440
LIG_SH3_3	153	159	PF00018	0.599
LIG_SH3_3	232	238	PF00018	0.475
LIG_SH3_3	260	266	PF00018	0.557
LIG_SH3_3	293	299	PF00018	0.446
LIG_SH3_3	486	492	PF00018	0.549
LIG_SH3_3	752	758	PF00018	0.460
LIG_SUMO_SIM_anti_2	175	180	PF11976	0.433
LIG_SUMO_SIM_anti_2	208	214	PF11976	0.353
LIG_SUMO_SIM_anti_2	68	74	PF11976	0.376
LIG_SUMO_SIM_par_1	201	208	PF11976	0.482
LIG_SUMO_SIM_par_1	238	245	PF11976	0.396
LIG_SUMO_SIM_par_1	50	56	PF11976	0.376
LIG_SUMO_SIM_par_1	67	74	PF11976	0.201
LIG_TRAF2_1	116	119	PF00917	0.376
LIG_TRAF2_1	199	202	PF00917	0.516
LIG_TRAF2_1	330	333	PF00917	0.516
LIG_TRAF2_1	488	491	PF00917	0.575
LIG_TRAF2_1	698	701	PF00917	0.505
LIG_TRAF2_1	737	740	PF00917	0.397
LIG_TRAF2_1	768	771	PF00917	0.347
LIG_TYR_ITIM	312	317	PF00017	0.331
LIG_TYR_ITIM	530	535	PF00017	0.430
LIG_TYR_ITSM	169	176	PF00017	0.401
MOD_CDK_SPxxK_3	426	433	PF00069	0.388
MOD_CK1_1	183	189	PF00069	0.637
MOD_CK1_1	205	211	PF00069	0.504
MOD_CK1_1	220	226	PF00069	0.437
MOD_CK1_1	336	342	PF00069	0.590
MOD_CK1_1	418	424	PF00069	0.592
MOD_CK1_1	585	591	PF00069	0.610
MOD_CK1_1	616	622	PF00069	0.449
MOD_CK1_1	680	686	PF00069	0.494
MOD_CK1_1	696	702	PF00069	0.583
MOD_CK2_1	196	202	PF00069	0.532
MOD_CK2_1	325	331	PF00069	0.445
MOD_CK2_1	696	702	PF00069	0.518
MOD_GlcNHglycan	1	4	PF01048	0.736
MOD_GlcNHglycan	142	145	PF01048	0.584
MOD_GlcNHglycan	149	152	PF01048	0.532
MOD_GlcNHglycan	163	166	PF01048	0.570
MOD_GlcNHglycan	244	247	PF01048	0.414
MOD_GlcNHglycan	301	304	PF01048	0.457
MOD_GlcNHglycan	518	521	PF01048	0.426
MOD_GlcNHglycan	618	621	PF01048	0.481
MOD_GlcNHglycan	681	685	PF01048	0.542
MOD_GlcNHglycan	77	80	PF01048	0.304
MOD_GSK3_1	157	164	PF00069	0.636
MOD_GSK3_1	168	175	PF00069	0.540
MOD_GSK3_1	179	186	PF00069	0.603
MOD_GSK3_1	190	197	PF00069	0.646
MOD_GSK3_1	333	340	PF00069	0.571
MOD_GSK3_1	638	645	PF00069	0.492
MOD_GSK3_1	723	730	PF00069	0.618
MOD_GSK3_1	756	763	PF00069	0.444
MOD_GSK3_1	88	95	PF00069	0.340
MOD_N-GLC_1	179	184	PF02516	0.576
MOD_N-GLC_1	191	196	PF02516	0.573
MOD_NEK2_1	1	6	PF00069	0.611
MOD_NEK2_1	179	184	PF00069	0.731
MOD_NEK2_1	225	230	PF00069	0.451
MOD_NEK2_1	241	246	PF00069	0.394
MOD_NEK2_1	40	45	PF00069	0.378
MOD_NEK2_1	425	430	PF00069	0.476
MOD_NEK2_1	48	53	PF00069	0.320
MOD_NEK2_1	525	530	PF00069	0.436
MOD_NEK2_1	599	604	PF00069	0.375
MOD_NEK2_1	682	687	PF00069	0.452
MOD_NEK2_1	717	722	PF00069	0.673
MOD_NEK2_1	97	102	PF00069	0.359
MOD_PIKK_1	264	270	PF00454	0.533
MOD_PIKK_1	693	699	PF00454	0.498
MOD_PKA_2	242	248	PF00069	0.470
MOD_PKA_2	336	342	PF00069	0.559
MOD_PKA_2	585	591	PF00069	0.439
MOD_PKB_1	335	343	PF00069	0.540
MOD_Plk_1	40	46	PF00069	0.243
MOD_Plk_1	717	723	PF00069	0.676
MOD_Plk_4	205	211	PF00069	0.388
MOD_Plk_4	220	226	PF00069	0.462
MOD_Plk_4	307	313	PF00069	0.371
MOD_Plk_4	341	347	PF00069	0.560
MOD_Plk_4	355	361	PF00069	0.420
MOD_Plk_4	526	532	PF00069	0.463
MOD_Plk_4	57	63	PF00069	0.360
MOD_Plk_4	585	591	PF00069	0.576
MOD_Plk_4	67	73	PF00069	0.360
MOD_Plk_4	97	103	PF00069	0.470
MOD_ProDKin_1	194	200	PF00069	0.639
MOD_ProDKin_1	217	223	PF00069	0.419
MOD_ProDKin_1	418	424	PF00069	0.496
MOD_ProDKin_1	426	432	PF00069	0.443
MOD_ProDKin_1	440	446	PF00069	0.303
MOD_ProDKin_1	92	98	PF00069	0.353
MOD_SUMO_rev_2	331	336	PF00179	0.558
MOD_SUMO_rev_2	483	488	PF00179	0.564
MOD_SUMO_rev_2	565	570	PF00179	0.411
TRG_DiLeu_BaEn_1	690	695	PF01217	0.461
TRG_DiLeu_BaEn_1	771	776	PF01217	0.343
TRG_DiLeu_BaEn_4	201	207	PF01217	0.419
TRG_DiLeu_BaEn_4	565	571	PF01217	0.421
TRG_DiLeu_BaLyEn_6	593	598	PF01217	0.431
TRG_ENDOCYTIC_2	173	176	PF00928	0.521
TRG_ENDOCYTIC_2	216	219	PF00928	0.440
TRG_ENDOCYTIC_2	314	317	PF00928	0.330
TRG_ENDOCYTIC_2	353	356	PF00928	0.368
TRG_ENDOCYTIC_2	532	535	PF00928	0.430
TRG_ENDOCYTIC_2	601	604	PF00928	0.276
TRG_ER_diArg_1	322	324	PF00400	0.445
TRG_ER_diArg_1	364	366	PF00400	0.426
TRG_ER_diArg_1	629	632	PF00400	0.430
TRG_Pf-PMV_PEXEL_1	533	537	PF00026	0.443
TRG_Pf-PMV_PEXEL_1	731	736	PF00026	0.377

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5J2	Leptomonas seymouri	51%	100%
A0A0S4JRQ2	Bodo saltans	23%	97%
A4HLX6	Leishmania braziliensis	71%	98%
A4I9A5	Leishmania infantum	100%	100%
E9B4A5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	99%
Q4Q3S1	Leishmania major	85%	98%