Diphthamide synthesis protein, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

diphthamide biosynthesis enzyme Dph1/Dph2 domain containing protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X6Z1_LEIDO

TriTrypDb:

LdBPK_332540.1 , LdCL_330032500

Length:

667

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S7X6Z1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X6Z1

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	11
GO:0006448	regulation of translational elongation	7	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009889	regulation of biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0010468	regulation of gene expression	5	11
GO:0010556	regulation of macromolecule biosynthetic process	5	11
GO:0010608	post-transcriptional regulation of gene expression	6	11
GO:0017182	peptidyl-diphthamide metabolic process	7	11
GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine	4	11
GO:0018193	peptidyl-amino acid modification	5	11
GO:0018202	peptidyl-histidine modification	6	11
GO:0019222	regulation of metabolic process	3	11
GO:0019538	protein metabolic process	3	11
GO:0031323	regulation of cellular metabolic process	4	11
GO:0031326	regulation of cellular biosynthetic process	5	11
GO:0034248	regulation of amide metabolic process	5	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0050789	regulation of biological process	2	11
GO:0050794	regulation of cellular process	3	11
GO:0051171	regulation of nitrogen compound metabolic process	4	11
GO:0051246	regulation of protein metabolic process	5	11
GO:0060255	regulation of macromolecule metabolic process	4	11
GO:0065007	biological regulation	1	11
GO:0071704	organic substance metabolic process	2	11
GO:0080090	regulation of primary metabolic process	4	11
GO:1900247	regulation of cytoplasmic translational elongation	8	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0016740	transferase activity	2	11
GO:0016765	transferase activity, transferring alkyl or aryl (other than methyl) groups	3	11
GO:0090560	2-(3-amino-3-carboxypropyl)histidine synthase activity	4	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	161	165	PF00656	0.354
CLV_C14_Caspase3-7	387	391	PF00656	0.489
CLV_C14_Caspase3-7	508	512	PF00656	0.516
CLV_MEL_PAP_1	628	634	PF00089	0.663
CLV_NRD_NRD_1	227	229	PF00675	0.458
CLV_NRD_NRD_1	422	424	PF00675	0.350
CLV_NRD_NRD_1	563	565	PF00675	0.422
CLV_PCSK_KEX2_1	227	229	PF00082	0.488
CLV_PCSK_KEX2_1	422	424	PF00082	0.261
CLV_PCSK_KEX2_1	468	470	PF00082	0.261
CLV_PCSK_KEX2_1	509	511	PF00082	0.350
CLV_PCSK_KEX2_1	557	559	PF00082	0.516
CLV_PCSK_KEX2_1	563	565	PF00082	0.482
CLV_PCSK_PC1ET2_1	468	470	PF00082	0.261
CLV_PCSK_PC1ET2_1	509	511	PF00082	0.350
CLV_PCSK_PC1ET2_1	557	559	PF00082	0.548
CLV_PCSK_PC7_1	464	470	PF00082	0.261
CLV_PCSK_SKI1_1	2	6	PF00082	0.363
CLV_PCSK_SKI1_1	78	82	PF00082	0.602
CLV_Separin_Metazoa	172	176	PF03568	0.348
DEG_Nend_UBRbox_1	1	4	PF02207	0.323
DEG_SPOP_SBC_1	269	273	PF00917	0.586
DEG_SPOP_SBC_1	289	293	PF00917	0.394
DOC_MAPK_gen_1	468	475	PF00069	0.460
DOC_MAPK_MEF2A_6	435	444	PF00069	0.460
DOC_MAPK_RevD_3	551	564	PF00069	0.355
DOC_PP2B_PxIxI_1	207	213	PF00149	0.375
DOC_PP4_FxxP_1	513	516	PF00568	0.475
DOC_USP7_MATH_1	126	130	PF00917	0.478
DOC_USP7_MATH_1	261	265	PF00917	0.639
DOC_USP7_MATH_1	309	313	PF00917	0.462
DOC_USP7_MATH_1	343	347	PF00917	0.494
DOC_USP7_MATH_1	378	382	PF00917	0.439
DOC_USP7_MATH_1	526	530	PF00917	0.550
DOC_USP7_MATH_1	562	566	PF00917	0.496
DOC_USP7_MATH_1	74	78	PF00917	0.633
DOC_WW_Pin1_4	516	521	PF00397	0.460
DOC_WW_Pin1_4	632	637	PF00397	0.527
LIG_14-3-3_CanoR_1	175	185	PF00244	0.437
LIG_14-3-3_CanoR_1	227	237	PF00244	0.511
LIG_14-3-3_CanoR_1	247	252	PF00244	0.616
LIG_14-3-3_CanoR_1	288	297	PF00244	0.614
LIG_14-3-3_CanoR_1	415	421	PF00244	0.492
LIG_14-3-3_CanoR_1	435	444	PF00244	0.438
LIG_14-3-3_CanoR_1	588	596	PF00244	0.667
LIG_14-3-3_CanoR_1	78	83	PF00244	0.657
LIG_deltaCOP1_diTrp_1	277	286	PF00928	0.447
LIG_FHA_1	148	154	PF00498	0.588
LIG_FHA_1	216	222	PF00498	0.473
LIG_FHA_1	387	393	PF00498	0.502
LIG_FHA_1	454	460	PF00498	0.475
LIG_FHA_2	159	165	PF00498	0.323
LIG_FHA_2	229	235	PF00498	0.575
LIG_FHA_2	526	532	PF00498	0.542
LIG_FHA_2	572	578	PF00498	0.622
LIG_GBD_Chelix_1	177	185	PF00786	0.382
LIG_LIR_Apic_2	511	516	PF02991	0.476
LIG_LIR_Gen_1	300	310	PF02991	0.461
LIG_LIR_Gen_1	322	332	PF02991	0.485
LIG_LIR_Gen_1	491	499	PF02991	0.460
LIG_LIR_Gen_1	546	556	PF02991	0.384
LIG_LIR_Nem_3	240	244	PF02991	0.356
LIG_LIR_Nem_3	300	306	PF02991	0.456
LIG_LIR_Nem_3	322	327	PF02991	0.518
LIG_LIR_Nem_3	37	41	PF02991	0.432
LIG_LIR_Nem_3	450	455	PF02991	0.516
LIG_LIR_Nem_3	470	474	PF02991	0.350
LIG_LIR_Nem_3	491	496	PF02991	0.460
LIG_LIR_Nem_3	546	552	PF02991	0.379
LIG_LIR_Nem_3	622	628	PF02991	0.544
LIG_NRBOX	458	464	PF00104	0.475
LIG_SH2_PTP2	138	141	PF00017	0.550
LIG_SH2_PTP2	625	628	PF00017	0.632
LIG_SH2_SRC	32	35	PF00017	0.545
LIG_SH2_STAP1	545	549	PF00017	0.399
LIG_SH2_STAT5	115	118	PF00017	0.537
LIG_SH2_STAT5	138	141	PF00017	0.460
LIG_SH2_STAT5	303	306	PF00017	0.367
LIG_SH2_STAT5	32	35	PF00017	0.545
LIG_SH2_STAT5	336	339	PF00017	0.420
LIG_SH2_STAT5	474	477	PF00017	0.468
LIG_SH2_STAT5	545	548	PF00017	0.482
LIG_SH2_STAT5	625	628	PF00017	0.632
LIG_SH3_3	202	208	PF00018	0.572
LIG_SH3_3	281	287	PF00018	0.485
LIG_SH3_3	493	499	PF00018	0.550
LIG_SH3_3	54	60	PF00018	0.701
LIG_SH3_3	82	88	PF00018	0.683
LIG_SUMO_SIM_anti_2	14	20	PF11976	0.411
LIG_SUMO_SIM_par_1	522	529	PF11976	0.492
MOD_CK1_1	129	135	PF00069	0.311
MOD_CK1_1	271	277	PF00069	0.608
MOD_CK1_1	291	297	PF00069	0.459
MOD_CK1_1	501	507	PF00069	0.372
MOD_CK1_1	599	605	PF00069	0.722
MOD_CK2_1	227	233	PF00069	0.609
MOD_CK2_1	516	522	PF00069	0.305
MOD_CK2_1	562	568	PF00069	0.619
MOD_Cter_Amidation	466	469	PF01082	0.305
MOD_GlcNHglycan	145	148	PF01048	0.530
MOD_GlcNHglycan	178	181	PF01048	0.519
MOD_GlcNHglycan	198	201	PF01048	0.541
MOD_GlcNHglycan	262	266	PF01048	0.655
MOD_GlcNHglycan	273	276	PF01048	0.643
MOD_GlcNHglycan	294	297	PF01048	0.648
MOD_GlcNHglycan	312	315	PF01048	0.402
MOD_GlcNHglycan	382	385	PF01048	0.506
MOD_GlcNHglycan	396	399	PF01048	0.414
MOD_GlcNHglycan	437	440	PF01048	0.324
MOD_GlcNHglycan	528	531	PF01048	0.324
MOD_GlcNHglycan	591	594	PF01048	0.593
MOD_GlcNHglycan	601	604	PF01048	0.586
MOD_GlcNHglycan	663	666	PF01048	0.747
MOD_GlcNHglycan	89	92	PF01048	0.582
MOD_GSK3_1	143	150	PF00069	0.391
MOD_GSK3_1	228	235	PF00069	0.505
MOD_GSK3_1	288	295	PF00069	0.601
MOD_GSK3_1	378	385	PF00069	0.567
MOD_GSK3_1	526	533	PF00069	0.396
MOD_GSK3_1	562	569	PF00069	0.549
MOD_GSK3_1	571	578	PF00069	0.641
MOD_GSK3_1	74	81	PF00069	0.639
MOD_LATS_1	76	82	PF00433	0.602
MOD_N-GLC_1	369	374	PF02516	0.479
MOD_NEK2_1	128	133	PF00069	0.309
MOD_NEK2_1	143	148	PF00069	0.367
MOD_NEK2_1	290	295	PF00069	0.641
MOD_NEK2_1	443	448	PF00069	0.404
MOD_PIKK_1	453	459	PF00454	0.429
MOD_PIKK_1	530	536	PF00454	0.547
MOD_PIKK_1	575	581	PF00454	0.702
MOD_PIKK_1	610	616	PF00454	0.604
MOD_PK_1	247	253	PF00069	0.622
MOD_PKA_1	227	233	PF00069	0.433
MOD_PKA_2	129	135	PF00069	0.314
MOD_PKA_2	183	189	PF00069	0.509
MOD_PKA_2	227	233	PF00069	0.521
MOD_PKA_2	434	440	PF00069	0.324
MOD_PKA_2	501	507	PF00069	0.372
MOD_PKA_2	562	568	PF00069	0.576
MOD_PKA_2	587	593	PF00069	0.662
MOD_PKA_2	596	602	PF00069	0.568
MOD_Plk_1	232	238	PF00069	0.517
MOD_Plk_1	98	104	PF00069	0.312
MOD_Plk_4	158	164	PF00069	0.411
MOD_ProDKin_1	516	522	PF00069	0.305
MOD_ProDKin_1	632	638	PF00069	0.530
MOD_SUMO_rev_2	34	41	PF00179	0.598
TRG_DiLeu_BaEn_1	300	305	PF01217	0.370
TRG_DiLeu_BaLyEn_6	551	556	PF01217	0.356
TRG_ENDOCYTIC_2	303	306	PF00928	0.400
TRG_ENDOCYTIC_2	324	327	PF00928	0.404
TRG_ENDOCYTIC_2	329	332	PF00928	0.412
TRG_ENDOCYTIC_2	452	455	PF00928	0.362
TRG_ENDOCYTIC_2	625	628	PF00928	0.632
TRG_ER_diArg_1	181	184	PF00400	0.405
TRG_ER_diArg_1	206	209	PF00400	0.343
TRG_ER_diArg_1	227	229	PF00400	0.488
TRG_ER_diArg_1	422	425	PF00400	0.331

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8D8	Leptomonas seymouri	55%	86%
A0A1X0P3T2	Trypanosomatidae	39%	100%
A0A3S5ISG6	Trypanosoma rangeli	41%	100%
A4HLV3	Leishmania braziliensis	73%	100%
D0A670	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AHQ9	Leishmania infantum	98%	100%
E9B480	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4Q3U6	Leishmania major	92%	100%
V5BP75	Trypanosoma cruzi	40%	100%