tRNA pseudouridine synthase D (TruD), putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase D (TruD), putative

Species:

Leishmania donovani

UniProt:

A0A3S7X658_LEIDO

TriTrypDb:

LdBPK_323580.1 , LdCL_320041700 , LDHU3_32.4520

Length:

717

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 13
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A0A3S7X658

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X658

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	15
GO:0006139	nucleobase-containing compound metabolic process	3	15
GO:0006725	cellular aromatic compound metabolic process	3	15
GO:0006807	nitrogen compound metabolic process	2	15
GO:0008152	metabolic process	1	15
GO:0009451	RNA modification	5	15
GO:0009987	cellular process	1	15
GO:0016070	RNA metabolic process	5	15
GO:0034641	cellular nitrogen compound metabolic process	3	15
GO:0043170	macromolecule metabolic process	3	15
GO:0043412	macromolecule modification	4	15
GO:0044237	cellular metabolic process	2	15
GO:0044238	primary metabolic process	2	15
GO:0046483	heterocycle metabolic process	3	15
GO:0071704	organic substance metabolic process	2	15
GO:0090304	nucleic acid metabolic process	4	15
GO:1901360	organic cyclic compound metabolic process	3	15
GO:0006396	RNA processing	6	3
GO:0006399	tRNA metabolic process	7	3
GO:0008033	tRNA processing	8	3
GO:0034470	ncRNA processing	7	3
GO:0034660	ncRNA metabolic process	6	3

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	15
GO:0003723	RNA binding	4	15
GO:0003824	catalytic activity	1	15
GO:0005488	binding	1	15
GO:0009982	pseudouridine synthase activity	4	15
GO:0016853	isomerase activity	2	15
GO:0016866	intramolecular transferase activity	3	15
GO:0097159	organic cyclic compound binding	2	15
GO:1901363	heterocyclic compound binding	2	15
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1
GO:0016829	lyase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	178	182	PF00656	0.533
CLV_C14_Caspase3-7	29	33	PF00656	0.568
CLV_NRD_NRD_1	124	126	PF00675	0.673
CLV_NRD_NRD_1	133	135	PF00675	0.634
CLV_NRD_NRD_1	149	151	PF00675	0.403
CLV_NRD_NRD_1	157	159	PF00675	0.432
CLV_NRD_NRD_1	196	198	PF00675	0.730
CLV_NRD_NRD_1	206	208	PF00675	0.682
CLV_NRD_NRD_1	240	242	PF00675	0.277
CLV_NRD_NRD_1	359	361	PF00675	0.290
CLV_NRD_NRD_1	636	638	PF00675	0.433
CLV_NRD_NRD_1	671	673	PF00675	0.707
CLV_PCSK_FUR_1	194	198	PF00082	0.760
CLV_PCSK_KEX2_1	124	126	PF00082	0.673
CLV_PCSK_KEX2_1	133	135	PF00082	0.656
CLV_PCSK_KEX2_1	149	151	PF00082	0.452
CLV_PCSK_KEX2_1	157	159	PF00082	0.471
CLV_PCSK_KEX2_1	196	198	PF00082	0.682
CLV_PCSK_KEX2_1	206	208	PF00082	0.685
CLV_PCSK_KEX2_1	242	244	PF00082	0.237
CLV_PCSK_KEX2_1	273	275	PF00082	0.402
CLV_PCSK_KEX2_1	359	361	PF00082	0.274
CLV_PCSK_KEX2_1	636	638	PF00082	0.552
CLV_PCSK_KEX2_1	671	673	PF00082	0.663
CLV_PCSK_PC1ET2_1	242	244	PF00082	0.235
CLV_PCSK_PC1ET2_1	273	275	PF00082	0.326
CLV_PCSK_SKI1_1	251	255	PF00082	0.281
CLV_PCSK_SKI1_1	297	301	PF00082	0.335
CLV_PCSK_SKI1_1	382	386	PF00082	0.276
CLV_PCSK_SKI1_1	498	502	PF00082	0.328
CLV_PCSK_SKI1_1	593	597	PF00082	0.585
CLV_PCSK_SKI1_1	708	712	PF00082	0.321
CLV_PCSK_SKI1_1	86	90	PF00082	0.451
CLV_Separin_Metazoa	154	158	PF03568	0.447
DEG_APCC_DBOX_1	592	600	PF00400	0.348
DEG_APCC_DBOX_1	81	89	PF00400	0.446
DEG_MDM2_SWIB_1	422	430	PF02201	0.382
DEG_ODPH_VHL_1	571	584	PF01847	0.275
DEG_SPOP_SBC_1	558	562	PF00917	0.535
DOC_CYCLIN_RxL_1	293	302	PF00134	0.273
DOC_CYCLIN_yCln2_LP_2	311	317	PF00134	0.355
DOC_CYCLIN_yCln2_LP_2	601	607	PF00134	0.355
DOC_MAPK_gen_1	271	278	PF00069	0.472
DOC_MAPK_gen_1	636	643	PF00069	0.348
DOC_MAPK_MEF2A_6	688	695	PF00069	0.270
DOC_MAPK_NFAT4_5	688	696	PF00069	0.267
DOC_PP1_RVXF_1	347	354	PF00149	0.382
DOC_PP1_RVXF_1	621	628	PF00149	0.438
DOC_PP4_FxxP_1	695	698	PF00568	0.319
DOC_USP7_MATH_1	136	140	PF00917	0.668
DOC_USP7_MATH_1	141	145	PF00917	0.640
DOC_USP7_MATH_1	192	196	PF00917	0.530
DOC_USP7_MATH_1	317	321	PF00917	0.379
DOC_USP7_MATH_1	327	331	PF00917	0.375
DOC_USP7_MATH_1	429	433	PF00917	0.267
DOC_USP7_MATH_1	479	483	PF00917	0.353
DOC_USP7_MATH_1	515	519	PF00917	0.266
DOC_USP7_MATH_1	545	549	PF00917	0.528
DOC_USP7_MATH_1	553	557	PF00917	0.718
DOC_USP7_MATH_1	558	562	PF00917	0.650
DOC_USP7_MATH_1	595	599	PF00917	0.568
DOC_WW_Pin1_4	217	222	PF00397	0.554
DOC_WW_Pin1_4	361	366	PF00397	0.359
LIG_14-3-3_CanoR_1	274	279	PF00244	0.338
LIG_14-3-3_CanoR_1	280	284	PF00244	0.289
LIG_14-3-3_CanoR_1	297	306	PF00244	0.311
LIG_14-3-3_CanoR_1	326	336	PF00244	0.426
LIG_14-3-3_CanoR_1	405	413	PF00244	0.297
LIG_14-3-3_CanoR_1	468	472	PF00244	0.233
LIG_14-3-3_CanoR_1	626	633	PF00244	0.468
LIG_14-3-3_CanoR_1	63	67	PF00244	0.547
LIG_14-3-3_CanoR_1	688	692	PF00244	0.444
LIG_BIR_III_2	17	21	PF00653	0.478
LIG_BIR_III_2	218	222	PF00653	0.540
LIG_BRCT_BRCA1_1	112	116	PF00533	0.498
LIG_BRCT_BRCA1_1	517	521	PF00533	0.235
LIG_deltaCOP1_diTrp_1	624	633	PF00928	0.360
LIG_FHA_1	462	468	PF00498	0.386
LIG_FHA_1	499	505	PF00498	0.479
LIG_FHA_1	688	694	PF00498	0.437
LIG_FHA_1	702	708	PF00498	0.245
LIG_FHA_2	112	118	PF00498	0.617
LIG_FHA_2	176	182	PF00498	0.633
LIG_FHA_2	206	212	PF00498	0.740
LIG_FHA_2	286	292	PF00498	0.347
LIG_FHA_2	365	371	PF00498	0.388
LIG_LIR_Apic_2	694	698	PF02991	0.325
LIG_LIR_Gen_1	113	123	PF02991	0.524
LIG_LIR_Gen_1	320	329	PF02991	0.240
LIG_LIR_Gen_1	43	53	PF02991	0.423
LIG_LIR_Gen_1	439	448	PF02991	0.317
LIG_LIR_Gen_1	489	500	PF02991	0.285
LIG_LIR_Gen_1	575	584	PF02991	0.376
LIG_LIR_Gen_1	700	710	PF02991	0.270
LIG_LIR_Nem_3	113	119	PF02991	0.533
LIG_LIR_Nem_3	256	260	PF02991	0.326
LIG_LIR_Nem_3	320	324	PF02991	0.226
LIG_LIR_Nem_3	340	346	PF02991	0.396
LIG_LIR_Nem_3	394	400	PF02991	0.249
LIG_LIR_Nem_3	43	49	PF02991	0.437
LIG_LIR_Nem_3	439	444	PF02991	0.296
LIG_LIR_Nem_3	470	474	PF02991	0.356
LIG_LIR_Nem_3	489	495	PF02991	0.285
LIG_LIR_Nem_3	518	523	PF02991	0.370
LIG_LIR_Nem_3	575	579	PF02991	0.427
LIG_LIR_Nem_3	646	650	PF02991	0.492
LIG_LIR_Nem_3	700	705	PF02991	0.275
LIG_LIR_Nem_3	94	100	PF02991	0.507
LIG_LYPXL_S_1	159	163	PF13949	0.540
LIG_LYPXL_yS_3	160	163	PF13949	0.397
LIG_MLH1_MIPbox_1	517	521	PF16413	0.382
LIG_NRBOX	84	90	PF00104	0.399
LIG_Pex14_2	397	401	PF04695	0.248
LIG_Pex14_2	422	426	PF04695	0.375
LIG_SH2_CRK	321	325	PF00017	0.382
LIG_SH2_CRK	576	580	PF00017	0.477
LIG_SH2_CRK	702	706	PF00017	0.258
LIG_SH2_NCK_1	702	706	PF00017	0.285
LIG_SH2_PTP2	235	238	PF00017	0.276
LIG_SH2_SRC	235	238	PF00017	0.276
LIG_SH2_STAP1	321	325	PF00017	0.328
LIG_SH2_STAP1	576	580	PF00017	0.278
LIG_SH2_STAP1	702	706	PF00017	0.302
LIG_SH2_STAT3	400	403	PF00017	0.248
LIG_SH2_STAT5	235	238	PF00017	0.276
LIG_SH2_STAT5	257	260	PF00017	0.326
LIG_SH2_STAT5	400	403	PF00017	0.277
LIG_SH2_STAT5	46	49	PF00017	0.404
LIG_SH2_STAT5	474	477	PF00017	0.388
LIG_SH2_STAT5	507	510	PF00017	0.352
LIG_SH2_STAT5	527	530	PF00017	0.159
LIG_SH3_1	125	131	PF00018	0.520
LIG_SH3_2	128	133	PF14604	0.495
LIG_SH3_3	125	131	PF00018	0.590
LIG_SH3_3	16	22	PF00018	0.526
LIG_SH3_3	311	317	PF00018	0.240
LIG_SH3_3	567	573	PF00018	0.475
LIG_SH3_3	579	585	PF00018	0.451
LIG_SH3_3	638	644	PF00018	0.338
LIG_SH3_3	648	654	PF00018	0.296
LIG_TRAF2_1	71	74	PF00917	0.432
LIG_TRFH_1	695	699	PF08558	0.402
LIG_TYR_ITIM	574	579	PF00017	0.280
LIG_WW_2	19	22	PF00397	0.486
MOD_CK1_1	115	121	PF00069	0.520
MOD_CK1_1	220	226	PF00069	0.625
MOD_CK1_1	328	334	PF00069	0.337
MOD_CK1_1	36	42	PF00069	0.666
MOD_CK1_1	364	370	PF00069	0.257
MOD_CK1_1	454	460	PF00069	0.259
MOD_CK1_1	561	567	PF00069	0.722
MOD_CK1_1	675	681	PF00069	0.439
MOD_CK1_1	91	97	PF00069	0.474
MOD_CK2_1	115	121	PF00069	0.615
MOD_CK2_1	205	211	PF00069	0.591
MOD_CK2_1	285	291	PF00069	0.347
MOD_CK2_1	364	370	PF00069	0.377
MOD_CK2_1	562	568	PF00069	0.542
MOD_CMANNOS	610	613	PF00535	0.459
MOD_GlcNHglycan	222	225	PF01048	0.619
MOD_GlcNHglycan	229	232	PF01048	0.267
MOD_GlcNHglycan	29	32	PF01048	0.663
MOD_GlcNHglycan	327	330	PF01048	0.303
MOD_GlcNHglycan	339	342	PF01048	0.320
MOD_GlcNHglycan	481	484	PF01048	0.386
MOD_GlcNHglycan	546	550	PF01048	0.777
MOD_GlcNHglycan	555	558	PF01048	0.643
MOD_GlcNHglycan	561	564	PF01048	0.661
MOD_GlcNHglycan	605	608	PF01048	0.506
MOD_GlcNHglycan	628	631	PF01048	0.514
MOD_GlcNHglycan	674	677	PF01048	0.529
MOD_GlcNHglycan	680	684	PF01048	0.728
MOD_GSK3_1	111	118	PF00069	0.541
MOD_GSK3_1	223	230	PF00069	0.476
MOD_GSK3_1	486	493	PF00069	0.370
MOD_GSK3_1	523	530	PF00069	0.403
MOD_GSK3_1	553	560	PF00069	0.723
MOD_GSK3_1	595	602	PF00069	0.536
MOD_GSK3_1	675	682	PF00069	0.641
MOD_GSK3_1	687	694	PF00069	0.380
MOD_GSK3_1	697	704	PF00069	0.327
MOD_N-GLC_1	183	188	PF02516	0.564
MOD_N-GLC_1	33	38	PF02516	0.527
MOD_N-GLC_1	486	491	PF02516	0.362
MOD_NEK2_1	111	116	PF00069	0.517
MOD_NEK2_1	325	330	PF00069	0.320
MOD_NEK2_1	406	411	PF00069	0.447
MOD_NEK2_1	452	457	PF00069	0.400
MOD_NEK2_1	467	472	PF00069	0.217
MOD_NEK2_1	49	54	PF00069	0.408
MOD_NEK2_1	523	528	PF00069	0.310
MOD_NEK2_1	625	630	PF00069	0.373
MOD_NEK2_1	691	696	PF00069	0.304
MOD_NEK2_2	170	175	PF00069	0.484
MOD_NEK2_2	429	434	PF00069	0.382
MOD_NEK2_2	515	520	PF00069	0.382
MOD_NEK2_2	595	600	PF00069	0.353
MOD_NEK2_2	701	706	PF00069	0.387
MOD_PIKK_1	666	672	PF00454	0.412
MOD_PK_1	261	267	PF00069	0.243
MOD_PK_1	274	280	PF00069	0.216
MOD_PKA_2	205	211	PF00069	0.703
MOD_PKA_2	279	285	PF00069	0.270
MOD_PKA_2	325	331	PF00069	0.435
MOD_PKA_2	404	410	PF00069	0.266
MOD_PKA_2	467	473	PF00069	0.303
MOD_PKA_2	62	68	PF00069	0.610
MOD_PKA_2	625	631	PF00069	0.469
MOD_PKA_2	652	658	PF00069	0.372
MOD_PKA_2	687	693	PF00069	0.469
MOD_Plk_1	183	189	PF00069	0.564
MOD_Plk_1	261	267	PF00069	0.226
MOD_Plk_1	33	39	PF00069	0.522
MOD_Plk_1	655	661	PF00069	0.428
MOD_Plk_1	73	79	PF00069	0.613
MOD_Plk_4	33	39	PF00069	0.653
MOD_Plk_4	515	521	PF00069	0.293
MOD_Plk_4	523	529	PF00069	0.293
MOD_Plk_4	701	707	PF00069	0.310
MOD_ProDKin_1	217	223	PF00069	0.552
MOD_ProDKin_1	361	367	PF00069	0.359
TRG_DiLeu_BaEn_1	249	254	PF01217	0.266
TRG_DiLeu_BaEn_1	84	89	PF01217	0.393
TRG_DiLeu_BaEn_3	379	385	PF01217	0.226
TRG_DiLeu_BaEn_4	376	382	PF01217	0.198
TRG_ENDOCYTIC_2	160	163	PF00928	0.397
TRG_ENDOCYTIC_2	235	238	PF00928	0.376
TRG_ENDOCYTIC_2	257	260	PF00928	0.326
TRG_ENDOCYTIC_2	321	324	PF00928	0.237
TRG_ENDOCYTIC_2	441	444	PF00928	0.248
TRG_ENDOCYTIC_2	46	49	PF00928	0.404
TRG_ENDOCYTIC_2	576	579	PF00928	0.477
TRG_ENDOCYTIC_2	702	705	PF00928	0.262
TRG_ER_diArg_1	100	103	PF00400	0.548
TRG_ER_diArg_1	123	125	PF00400	0.660
TRG_ER_diArg_1	133	135	PF00400	0.695
TRG_ER_diArg_1	148	150	PF00400	0.430
TRG_ER_diArg_1	156	158	PF00400	0.407
TRG_ER_diArg_1	194	197	PF00400	0.697
TRG_ER_diArg_1	206	209	PF00400	0.625
TRG_ER_diArg_1	240	243	PF00400	0.275
TRG_ER_diArg_1	346	349	PF00400	0.331
TRG_ER_diArg_1	359	361	PF00400	0.345
TRG_ER_diArg_1	636	638	PF00400	0.547
TRG_ER_diArg_1	671	673	PF00400	0.441
TRG_NES_CRM1_1	249	262	PF08389	0.291
TRG_NLS_MonoExtC_3	240	246	PF00514	0.248
TRG_Pf-PMV_PEXEL_1	251	256	PF00026	0.266
TRG_Pf-PMV_PEXEL_1	297	302	PF00026	0.184
TRG_Pf-PMV_PEXEL_1	75	79	PF00026	0.578

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4F4	Leptomonas seymouri	51%	75%
A0A0S4JDL8	Bodo saltans	27%	100%
A0A1X0NRV3	Trypanosomatidae	33%	100%
A0A3Q8IJ87	Leishmania donovani	23%	100%
A0A3R7LD82	Trypanosoma rangeli	33%	100%
A4HKY3	Leishmania braziliensis	74%	100%
A4I8G2	Leishmania infantum	100%	100%
A4IDY9	Leishmania infantum	23%	100%
D0AAQ9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9B3C1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
Q08647	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	22%	100%
Q1L8I0	Danio rerio	23%	100%
Q4Q0I6	Leishmania major	23%	100%
Q4Q4T4	Leishmania major	92%	99%
V5BLA6	Trypanosoma cruzi	32%	100%