Ergosterol biosynthesis ERG4/ERG24 family protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

C-14 sterol reductase, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X5R0_LEIDO

TriTrypDb:

LdBPK_322470.1 , LdCL_320030000 , LDHU3_32.3060

Length:

437

Annotations

Annotations by Jardim et al.

Sterol metabolism/biosynthesis, C-14 sterol reductase

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

A0A3S7X5R0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X5R0

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	12
GO:0006694	steroid biosynthetic process	5	12
GO:0008152	metabolic process	1	12
GO:0008202	steroid metabolic process	4	12
GO:0008610	lipid biosynthetic process	4	12
GO:0009058	biosynthetic process	2	12
GO:0016125	sterol metabolic process	4	12
GO:0016126	sterol biosynthetic process	5	12
GO:0044238	primary metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12
GO:1901615	organic hydroxy compound metabolic process	3	12
GO:1901617	organic hydroxy compound biosynthetic process	4	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0016491	oxidoreductase activity	2	12
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	12
GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor	4	12
GO:0050613	delta14-sterol reductase activity	5	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	332	334	PF00675	0.298
CLV_PCSK_SKI1_1	175	179	PF00082	0.311
CLV_PCSK_SKI1_1	345	349	PF00082	0.216
CLV_PCSK_SKI1_1	426	430	PF00082	0.256
DEG_APCC_DBOX_1	174	182	PF00400	0.511
DOC_CYCLIN_yCln2_LP_2	148	154	PF00134	0.337
DOC_MAPK_gen_1	348	357	PF00069	0.457
DOC_MAPK_MEF2A_6	78	85	PF00069	0.303
DOC_USP7_MATH_1	44	48	PF00917	0.311
DOC_WW_Pin1_4	235	240	PF00397	0.420
LIG_14-3-3_CanoR_1	19	24	PF00244	0.537
LIG_14-3-3_CanoR_1	323	332	PF00244	0.432
LIG_14-3-3_CanoR_1	345	355	PF00244	0.456
LIG_Actin_WH2_2	62	80	PF00022	0.224
LIG_Actin_WH2_2	95	111	PF00022	0.261
LIG_BIR_II_1	1	5	PF00653	0.681
LIG_CSL_BTD_1	283	286	PF09270	0.258
LIG_deltaCOP1_diTrp_1	281	287	PF00928	0.243
LIG_deltaCOP1_diTrp_1	372	379	PF00928	0.408
LIG_deltaCOP1_diTrp_1	387	392	PF00928	0.408
LIG_deltaCOP1_diTrp_1	419	424	PF00928	0.435
LIG_deltaCOP1_diTrp_1	84	94	PF00928	0.277
LIG_EH1_1	123	131	PF00400	0.266
LIG_eIF4E_1	251	257	PF01652	0.416
LIG_eIF4E_1	369	375	PF01652	0.418
LIG_eIF4E_1	396	402	PF01652	0.408
LIG_FHA_1	135	141	PF00498	0.307
LIG_FHA_1	200	206	PF00498	0.412
LIG_FHA_1	236	242	PF00498	0.418
LIG_FHA_1	286	292	PF00498	0.295
LIG_FHA_1	38	44	PF00498	0.389
LIG_FHA_2	325	331	PF00498	0.456
LIG_LIR_Apic_2	22	28	PF02991	0.409
LIG_LIR_Gen_1	244	253	PF02991	0.477
LIG_LIR_Gen_1	419	428	PF02991	0.456
LIG_LIR_LC3C_4	40	43	PF02991	0.255
LIG_LIR_Nem_3	147	152	PF02991	0.240
LIG_LIR_Nem_3	244	250	PF02991	0.472
LIG_LIR_Nem_3	281	287	PF02991	0.265
LIG_LIR_Nem_3	358	363	PF02991	0.403
LIG_LIR_Nem_3	385	391	PF02991	0.428
LIG_LIR_Nem_3	419	424	PF02991	0.422
LIG_MAD2	426	434	PF02301	0.475
LIG_NRBOX	252	258	PF00104	0.511
LIG_PCNA_PIPBox_1	142	151	PF02747	0.256
LIG_Pex14_1	388	392	PF04695	0.543
LIG_Pex14_2	132	136	PF04695	0.266
LIG_Pex14_2	275	279	PF04695	0.243
LIG_Pex14_2	33	37	PF04695	0.391
LIG_PTB_Apo_2	221	228	PF02174	0.408
LIG_SH2_CRK	149	153	PF00017	0.382
LIG_SH2_STAP1	168	172	PF00017	0.365
LIG_SH2_STAP1	190	194	PF00017	0.408
LIG_SH2_STAT3	289	292	PF00017	0.357
LIG_SH2_STAT5	251	254	PF00017	0.416
LIG_SH2_STAT5	289	292	PF00017	0.259
LIG_SH2_STAT5	295	298	PF00017	0.208
LIG_SH2_STAT5	319	322	PF00017	0.296
LIG_SH2_STAT5	369	372	PF00017	0.420
LIG_SH2_STAT5	391	394	PF00017	0.426
LIG_SH2_STAT5	396	399	PF00017	0.404
LIG_SH3_1	333	339	PF00018	0.432
LIG_SH3_3	12	18	PF00018	0.532
LIG_SH3_3	294	300	PF00018	0.353
LIG_SH3_3	333	339	PF00018	0.432
LIG_SUMO_SIM_par_1	40	47	PF11976	0.285
LIG_TRFH_1	430	434	PF08558	0.243
LIG_UBA3_1	96	101	PF00899	0.328
LIG_WRC_WIRS_1	158	163	PF05994	0.415
LIG_WRC_WIRS_1	247	252	PF05994	0.302
MOD_CK1_1	171	177	PF00069	0.304
MOD_CK2_1	6	12	PF00069	0.685
MOD_GlcNHglycan	109	112	PF01048	0.393
MOD_GlcNHglycan	184	187	PF01048	0.309
MOD_GlcNHglycan	314	317	PF01048	0.243
MOD_GlcNHglycan	357	360	PF01048	0.293
MOD_GlcNHglycan	382	385	PF01048	0.278
MOD_GSK3_1	157	164	PF00069	0.260
MOD_GSK3_1	233	240	PF00069	0.246
MOD_GSK3_1	347	354	PF00069	0.246
MOD_N-GLC_1	261	266	PF02516	0.264
MOD_NEK2_1	134	139	PF00069	0.361
MOD_NEK2_1	161	166	PF00069	0.316
MOD_NEK2_1	246	251	PF00069	0.258
MOD_NEK2_1	287	292	PF00069	0.275
MOD_NEK2_1	310	315	PF00069	0.308
MOD_NEK2_1	347	352	PF00069	0.249
MOD_NEK2_1	355	360	PF00069	0.243
MOD_NEK2_1	76	81	PF00069	0.390
MOD_Plk_1	261	267	PF00069	0.263
MOD_Plk_4	135	141	PF00069	0.452
MOD_Plk_4	161	167	PF00069	0.296
MOD_Plk_4	184	190	PF00069	0.281
MOD_Plk_4	237	243	PF00069	0.250
MOD_Plk_4	246	252	PF00069	0.231
MOD_Plk_4	261	267	PF00069	0.259
MOD_Plk_4	301	307	PF00069	0.285
MOD_Plk_4	351	357	PF00069	0.258
MOD_Plk_4	37	43	PF00069	0.343
MOD_Plk_4	52	58	PF00069	0.219
MOD_ProDKin_1	235	241	PF00069	0.260
TRG_DiLeu_BaEn_1	52	57	PF01217	0.406
TRG_ENDOCYTIC_2	149	152	PF00928	0.355
TRG_ENDOCYTIC_2	319	322	PF00928	0.291
TRG_ENDOCYTIC_2	360	363	PF00928	0.243
TRG_ENDOCYTIC_2	396	399	PF00928	0.269
TRG_ENDOCYTIC_2	430	433	PF00928	0.243
TRG_ER_diArg_1	172	175	PF00400	0.387
TRG_Pf-PMV_PEXEL_1	404	408	PF00026	0.243

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3C6	Leptomonas seymouri	25%	87%
A0A0N1PBH8	Leptomonas seymouri	76%	100%
A0A0S4IS37	Bodo saltans	59%	100%
A0A0S4KLN5	Bodo saltans	25%	93%
A0A1D8PIC7	Candida albicans (strain SC5314 / ATCC MYA-2876)	36%	98%
A0A1D8PJ25	Candida albicans (strain SC5314 / ATCC MYA-2876)	26%	93%
A0A1X0NRH6	Trypanosomatidae	60%	95%
A0A1X0P0Q6	Trypanosomatidae	28%	88%
A0A3Q8IM20	Leishmania donovani	27%	100%
A0A3R7KUI7	Trypanosoma rangeli	28%	90%
A0A3R7N804	Trypanosoma rangeli	59%	96%
A4HKM3	Leishmania braziliensis	86%	100%
A4HLA8	Leishmania braziliensis	26%	100%
A4I855	Leishmania infantum	100%	100%
A4I8T4	Leishmania infantum	27%	100%
D0AAC2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	95%
E9B312	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
E9B3Q0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
G4SW86	Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z)	38%	100%
I1RF79	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	34%	91%
I1RR90	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	34%	90%
I1RZZ3	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	29%	73%
O08984	Rattus norvegicus	42%	70%
O13597	Septoria lycopersici	34%	85%
O76062	Homo sapiens	47%	100%
O88455	Mus musculus	30%	93%
P23913	Gallus gallus	40%	69%
P25340	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	92%
P32462	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	36%	100%
P36209	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	96%
P38670	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	37%	89%
P78575	Ascobolus immersus	40%	100%
Q01447	Fusarium vanettenii	35%	90%
Q09195	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	100%
Q14739	Homo sapiens	41%	71%
Q3U9G9	Mus musculus	42%	70%
Q4Q4D7	Leishmania major	26%	100%
Q4Q543	Leishmania major	95%	100%
Q4WJ59	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	28%	77%
Q4WJJ9	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	39%	84%
Q4WKA5	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	36%	88%
Q4WW43	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	29%	93%
Q54PP1	Dictyostelium discoideum	33%	95%
Q5E9J5	Bos taurus	30%	92%
Q5R7H4	Pongo abelii	41%	71%
Q5UQI4	Acanthamoeba polyphaga mimivirus	31%	98%
Q6P4M0	Xenopus tropicalis	31%	92%
Q71KT5	Mus musculus	43%	100%
Q7SXF1	Danio rerio	30%	91%
Q7ZXH1	Xenopus laevis	33%	92%
Q8WMV1	Bos taurus	46%	100%
Q9LDR4	Arabidopsis thaliana	35%	100%
Q9LDU6	Arabidopsis thaliana	31%	100%
Q9UBM7	Homo sapiens	31%	92%
Q9Z2Z8	Rattus norvegicus	31%	93%
V5BKN3	Trypanosoma cruzi	57%	96%