ATP-dependent DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

PIF1 helicase-like protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X5K3_LEIDO

TriTrypDb:

LdBPK_110320.1 , LdCL_320022250 , LDHU3_11.0410

Length:

937

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	9
GO:0110165	cellular anatomical entity	1	9

Expansion

Sequence features

A0A3S7X5K3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X5K3

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	9
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006259	DNA metabolic process	4	9
GO:0006281	DNA repair	5	9
GO:0006310	DNA recombination	5	9
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0006950	response to stress	2	9
GO:0006974	DNA damage response	4	9
GO:0006996	organelle organization	4	9
GO:0008152	metabolic process	1	9
GO:0009987	cellular process	1	9
GO:0016043	cellular component organization	3	9
GO:0032200	telomere organization	6	9
GO:0033554	cellular response to stress	3	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0043170	macromolecule metabolic process	3	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0044260	obsolete cellular macromolecule metabolic process	3	9
GO:0046483	heterocycle metabolic process	3	9
GO:0050896	response to stimulus	1	9
GO:0051276	chromosome organization	5	9
GO:0051716	cellular response to stimulus	2	9
GO:0071704	organic substance metabolic process	2	9
GO:0071840	cellular component organization or biogenesis	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	9
GO:0003678	DNA helicase activity	3	9
GO:0003824	catalytic activity	1	9
GO:0004386	helicase activity	2	9
GO:0005488	binding	1	9
GO:0005524	ATP binding	5	9
GO:0008094	ATP-dependent activity, acting on DNA	2	9
GO:0016462	pyrophosphatase activity	5	9
GO:0016787	hydrolase activity	2	9
GO:0016817	hydrolase activity, acting on acid anhydrides	3	9
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	9
GO:0016887	ATP hydrolysis activity	7	9
GO:0017076	purine nucleotide binding	4	9
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	9
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:0140097	catalytic activity, acting on DNA	3	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:0140657	ATP-dependent activity	1	9
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	388	392	PF00656	0.486
CLV_C14_Caspase3-7	58	62	PF00656	0.776
CLV_C14_Caspase3-7	687	691	PF00656	0.351
CLV_NRD_NRD_1	263	265	PF00675	0.624
CLV_NRD_NRD_1	300	302	PF00675	0.459
CLV_NRD_NRD_1	339	341	PF00675	0.690
CLV_NRD_NRD_1	407	409	PF00675	0.762
CLV_NRD_NRD_1	423	425	PF00675	0.532
CLV_NRD_NRD_1	47	49	PF00675	0.612
CLV_NRD_NRD_1	504	506	PF00675	0.489
CLV_NRD_NRD_1	632	634	PF00675	0.531
CLV_NRD_NRD_1	696	698	PF00675	0.526
CLV_NRD_NRD_1	710	712	PF00675	0.449
CLV_NRD_NRD_1	873	875	PF00675	0.469
CLV_NRD_NRD_1	98	100	PF00675	0.789
CLV_PCSK_FUR_1	337	341	PF00082	0.724
CLV_PCSK_KEX2_1	263	265	PF00082	0.623
CLV_PCSK_KEX2_1	300	302	PF00082	0.459
CLV_PCSK_KEX2_1	337	339	PF00082	0.685
CLV_PCSK_KEX2_1	407	409	PF00082	0.768
CLV_PCSK_KEX2_1	423	425	PF00082	0.484
CLV_PCSK_KEX2_1	47	49	PF00082	0.612
CLV_PCSK_KEX2_1	632	634	PF00082	0.499
CLV_PCSK_KEX2_1	696	698	PF00082	0.487
CLV_PCSK_KEX2_1	98	100	PF00082	0.789
CLV_PCSK_PC1ET2_1	407	409	PF00082	0.801
CLV_PCSK_PC7_1	692	698	PF00082	0.612
CLV_PCSK_SKI1_1	127	131	PF00082	0.682
CLV_PCSK_SKI1_1	13	17	PF00082	0.640
CLV_PCSK_SKI1_1	432	436	PF00082	0.470
CLV_PCSK_SKI1_1	505	509	PF00082	0.496
CLV_PCSK_SKI1_1	567	571	PF00082	0.678
CLV_PCSK_SKI1_1	637	641	PF00082	0.506
CLV_PCSK_SKI1_1	692	696	PF00082	0.487
CLV_PCSK_SKI1_1	719	723	PF00082	0.422
CLV_PCSK_SKI1_1	726	730	PF00082	0.354
CLV_PCSK_SKI1_1	731	735	PF00082	0.393
CLV_PCSK_SKI1_1	755	759	PF00082	0.483
CLV_Separin_Metazoa	682	686	PF03568	0.594
DEG_APCC_DBOX_1	572	580	PF00400	0.559
DEG_Nend_UBRbox_1	1	4	PF02207	0.622
DEG_SCF_FBW7_1	323	330	PF00400	0.473
DOC_ANK_TNKS_1	341	348	PF00023	0.511
DOC_ANK_TNKS_1	796	803	PF00023	0.457
DOC_CKS1_1	205	210	PF01111	0.413
DOC_CKS1_1	324	329	PF01111	0.437
DOC_CKS1_1	831	836	PF01111	0.433
DOC_CYCLIN_RxL_1	258	270	PF00134	0.647
DOC_CYCLIN_RxL_1	749	761	PF00134	0.438
DOC_CYCLIN_yClb1_LxF_4	262	267	PF00134	0.567
DOC_CYCLIN_yCln2_LP_2	604	610	PF00134	0.630
DOC_MAPK_DCC_7	803	812	PF00069	0.362
DOC_MAPK_gen_1	575	584	PF00069	0.551
DOC_MAPK_gen_1	719	729	PF00069	0.382
DOC_MAPK_gen_1	872	881	PF00069	0.475
DOC_MAPK_MEF2A_6	803	812	PF00069	0.362
DOC_PP1_RVXF_1	261	268	PF00149	0.625
DOC_PP1_RVXF_1	742	749	PF00149	0.477
DOC_PP4_FxxP_1	201	204	PF00568	0.337
DOC_PP4_FxxP_1	559	562	PF00568	0.465
DOC_PP4_FxxP_1	925	928	PF00568	0.490
DOC_PP4_MxPP_1	527	530	PF00568	0.483
DOC_USP7_MATH_1	251	255	PF00917	0.489
DOC_USP7_MATH_1	28	32	PF00917	0.668
DOC_USP7_MATH_1	327	331	PF00917	0.444
DOC_USP7_MATH_1	368	372	PF00917	0.769
DOC_USP7_MATH_1	382	386	PF00917	0.674
DOC_USP7_MATH_1	392	396	PF00917	0.704
DOC_USP7_MATH_1	403	407	PF00917	0.585
DOC_USP7_MATH_1	562	566	PF00917	0.651
DOC_USP7_MATH_1	595	599	PF00917	0.566
DOC_USP7_MATH_1	649	653	PF00917	0.528
DOC_USP7_MATH_1	914	918	PF00917	0.397
DOC_USP7_UBL2_3	14	18	PF12436	0.607
DOC_USP7_UBL2_3	496	500	PF12436	0.562
DOC_WW_Pin1_4	204	209	PF00397	0.405
DOC_WW_Pin1_4	323	328	PF00397	0.437
DOC_WW_Pin1_4	359	364	PF00397	0.683
DOC_WW_Pin1_4	47	52	PF00397	0.618
DOC_WW_Pin1_4	558	563	PF00397	0.658
DOC_WW_Pin1_4	56	61	PF00397	0.571
DOC_WW_Pin1_4	603	608	PF00397	0.579
DOC_WW_Pin1_4	764	769	PF00397	0.559
DOC_WW_Pin1_4	830	835	PF00397	0.429
DOC_WW_Pin1_4	882	887	PF00397	0.432
LIG_14-3-3_CanoR_1	178	187	PF00244	0.393
LIG_14-3-3_CanoR_1	632	637	PF00244	0.457
LIG_14-3-3_CanoR_1	731	736	PF00244	0.544
LIG_14-3-3_CanoR_1	749	758	PF00244	0.551
LIG_14-3-3_CanoR_1	887	892	PF00244	0.475
LIG_Actin_WH2_2	691	709	PF00022	0.609
LIG_BIR_III_2	367	371	PF00653	0.512
LIG_BIR_III_4	799	803	PF00653	0.508
LIG_BRCT_BRCA1_1	307	311	PF00533	0.420
LIG_BRCT_BRCA1_1	318	322	PF00533	0.293
LIG_BRCT_BRCA1_1	436	440	PF00533	0.409
LIG_BRCT_BRCA1_1	894	898	PF00533	0.509
LIG_Clathr_ClatBox_1	683	687	PF01394	0.526
LIG_deltaCOP1_diTrp_1	426	435	PF00928	0.593
LIG_deltaCOP1_diTrp_1	436	440	PF00928	0.466
LIG_deltaCOP1_diTrp_1	820	823	PF00928	0.570
LIG_FHA_1	128	134	PF00498	0.705
LIG_FHA_1	14	20	PF00498	0.682
LIG_FHA_1	198	204	PF00498	0.362
LIG_FHA_1	268	274	PF00498	0.540
LIG_FHA_1	31	37	PF00498	0.712
LIG_FHA_1	374	380	PF00498	0.713
LIG_FHA_1	439	445	PF00498	0.422
LIG_FHA_1	623	629	PF00498	0.553
LIG_FHA_1	693	699	PF00498	0.565
LIG_FHA_1	809	815	PF00498	0.507
LIG_FHA_1	897	903	PF00498	0.520
LIG_FHA_2	113	119	PF00498	0.726
LIG_FHA_2	138	144	PF00498	0.496
LIG_FHA_2	205	211	PF00498	0.364
LIG_FHA_2	386	392	PF00498	0.571
LIG_FHA_2	520	526	PF00498	0.635
LIG_FHA_2	815	821	PF00498	0.508
LIG_IRF3_LxIS_1	808	815	PF10401	0.397
LIG_LIR_Apic_2	199	204	PF02991	0.338
LIG_LIR_Gen_1	319	329	PF02991	0.339
LIG_LIR_Nem_3	308	314	PF02991	0.405
LIG_LIR_Nem_3	319	325	PF02991	0.271
LIG_LIR_Nem_3	326	331	PF02991	0.200
LIG_LIR_Nem_3	641	646	PF02991	0.649
LIG_MYND_1	42	46	PF01753	0.616
LIG_PCNA_yPIPBox_3	739	749	PF02747	0.460
LIG_Pex14_2	197	201	PF04695	0.337
LIG_SH2_CRK	341	345	PF00017	0.512
LIG_SH2_CRK	430	434	PF00017	0.531
LIG_SH2_CRK	602	606	PF00017	0.522
LIG_SH2_GRB2like	737	740	PF00017	0.419
LIG_SH2_SRC	737	740	PF00017	0.419
LIG_SH2_SRC	926	929	PF00017	0.523
LIG_SH2_STAP1	610	614	PF00017	0.597
LIG_SH2_STAP1	665	669	PF00017	0.462
LIG_SH2_STAP1	836	840	PF00017	0.379
LIG_SH2_STAT5	737	740	PF00017	0.419
LIG_SH2_STAT5	867	870	PF00017	0.366
LIG_SH2_STAT5	926	929	PF00017	0.629
LIG_SH3_1	48	54	PF00018	0.510
LIG_SH3_2	42	47	PF14604	0.699
LIG_SH3_3	117	123	PF00018	0.703
LIG_SH3_3	200	206	PF00018	0.381
LIG_SH3_3	328	334	PF00018	0.640
LIG_SH3_3	39	45	PF00018	0.680
LIG_SH3_3	48	54	PF00018	0.788
LIG_SH3_3	788	794	PF00018	0.512
LIG_SH3_3	802	808	PF00018	0.322
LIG_SH3_3	828	834	PF00018	0.377
LIG_SH3_3	888	894	PF00018	0.488
LIG_SUMO_SIM_anti_2	275	280	PF11976	0.343
LIG_SUMO_SIM_par_1	277	283	PF11976	0.339
LIG_SUMO_SIM_par_1	506	511	PF11976	0.570
LIG_SUMO_SIM_par_1	811	817	PF11976	0.478
LIG_UBA3_1	164	171	PF00899	0.413
LIG_WRC_WIRS_1	292	297	PF05994	0.473
LIG_WRC_WIRS_1	748	753	PF05994	0.470
LIG_WW_2	42	45	PF00397	0.615
LIG_WW_3	44	48	PF00397	0.703
MOD_CDK_SPK_2	882	887	PF00069	0.489
MOD_CK1_1	114	120	PF00069	0.708
MOD_CK1_1	132	138	PF00069	0.376
MOD_CK1_1	362	368	PF00069	0.671
MOD_CK1_1	385	391	PF00069	0.675
MOD_CK1_1	412	418	PF00069	0.708
MOD_CK1_1	565	571	PF00069	0.591
MOD_CK1_1	606	612	PF00069	0.619
MOD_CK1_1	62	68	PF00069	0.615
MOD_CK1_1	622	628	PF00069	0.565
MOD_CK1_1	652	658	PF00069	0.667
MOD_CK1_1	82	88	PF00069	0.612
MOD_CK2_1	368	374	PF00069	0.763
MOD_CK2_1	392	398	PF00069	0.686
MOD_CK2_1	519	525	PF00069	0.592
MOD_CMANNOS	912	915	PF00535	0.405
MOD_Cter_Amidation	335	338	PF01082	0.734
MOD_Cter_Amidation	421	424	PF01082	0.663
MOD_GlcNHglycan	25	28	PF01048	0.709
MOD_GlcNHglycan	30	33	PF01048	0.597
MOD_GlcNHglycan	318	321	PF01048	0.362
MOD_GlcNHglycan	370	373	PF01048	0.668
MOD_GlcNHglycan	398	401	PF01048	0.728
MOD_GlcNHglycan	417	420	PF01048	0.672
MOD_GlcNHglycan	478	481	PF01048	0.439
MOD_GlcNHglycan	564	567	PF01048	0.659
MOD_GlcNHglycan	614	617	PF01048	0.539
MOD_GlcNHglycan	621	624	PF01048	0.587
MOD_GlcNHglycan	64	67	PF01048	0.763
MOD_GlcNHglycan	643	646	PF01048	0.631
MOD_GlcNHglycan	647	650	PF01048	0.703
MOD_GlcNHglycan	655	658	PF01048	0.677
MOD_GlcNHglycan	751	754	PF01048	0.579
MOD_GlcNHglycan	788	791	PF01048	0.546
MOD_GlcNHglycan	84	87	PF01048	0.784
MOD_GlcNHglycan	920	923	PF01048	0.637
MOD_GlcNHglycan	934	937	PF01048	0.567
MOD_GlcNHglycan	99	102	PF01048	0.602
MOD_GSK3_1	108	115	PF00069	0.702
MOD_GSK3_1	193	200	PF00069	0.369
MOD_GSK3_1	209	216	PF00069	0.690
MOD_GSK3_1	23	30	PF00069	0.788
MOD_GSK3_1	323	330	PF00069	0.407
MOD_GSK3_1	392	399	PF00069	0.687
MOD_GSK3_1	434	441	PF00069	0.435
MOD_GSK3_1	52	59	PF00069	0.760
MOD_GSK3_1	528	535	PF00069	0.620
MOD_GSK3_1	558	565	PF00069	0.663
MOD_GSK3_1	606	613	PF00069	0.561
MOD_GSK3_1	641	648	PF00069	0.559
MOD_GSK3_1	649	656	PF00069	0.662
MOD_GSK3_1	71	78	PF00069	0.702
MOD_GSK3_1	808	815	PF00069	0.559
MOD_GSK3_1	82	89	PF00069	0.540
MOD_GSK3_1	892	899	PF00069	0.474
MOD_GSK3_1	914	921	PF00069	0.545
MOD_N-GLC_1	731	736	PF02516	0.454
MOD_N-GLC_1	738	743	PF02516	0.395
MOD_N-GLC_1	882	887	PF02516	0.432
MOD_NEK2_1	197	202	PF00069	0.362
MOD_NEK2_1	267	272	PF00069	0.574
MOD_NEK2_1	434	439	PF00069	0.470
MOD_NEK2_1	476	481	PF00069	0.436
MOD_NEK2_1	532	537	PF00069	0.716
MOD_NEK2_1	608	613	PF00069	0.558
MOD_NEK2_1	702	707	PF00069	0.421
MOD_NEK2_1	896	901	PF00069	0.452
MOD_NEK2_2	193	198	PF00069	0.362
MOD_NEK2_2	32	37	PF00069	0.576
MOD_NEK2_2	382	387	PF00069	0.619
MOD_NEK2_2	836	841	PF00069	0.383
MOD_PIKK_1	13	19	PF00454	0.525
MOD_PIKK_1	213	219	PF00454	0.534
MOD_PIKK_1	305	311	PF00454	0.346
MOD_PIKK_1	516	522	PF00454	0.532
MOD_PIKK_1	79	85	PF00454	0.729
MOD_PIKK_1	896	902	PF00454	0.516
MOD_PK_1	2	8	PF00069	0.569
MOD_PK_1	887	893	PF00069	0.515
MOD_PKA_1	13	19	PF00069	0.579
MOD_PKA_1	409	415	PF00069	0.778
MOD_PKA_1	632	638	PF00069	0.463
MOD_PKA_1	711	717	PF00069	0.553
MOD_PKA_2	267	273	PF00069	0.576
MOD_PKA_2	409	415	PF00069	0.778
MOD_PKA_2	474	480	PF00069	0.457
MOD_PKA_2	595	601	PF00069	0.479
MOD_PKA_2	619	625	PF00069	0.544
MOD_PKA_2	632	638	PF00069	0.536
MOD_PKA_2	649	655	PF00069	0.454
MOD_PKA_2	892	898	PF00069	0.546
MOD_PKA_2	97	103	PF00069	0.619
MOD_Plk_1	282	288	PF00069	0.337
MOD_Plk_1	373	379	PF00069	0.610
MOD_Plk_1	731	737	PF00069	0.502
MOD_Plk_2-3	137	143	PF00069	0.473
MOD_Plk_4	129	135	PF00069	0.646
MOD_Plk_4	147	153	PF00069	0.308
MOD_Plk_4	282	288	PF00069	0.318
MOD_Plk_4	711	717	PF00069	0.548
MOD_Plk_4	892	898	PF00069	0.534
MOD_ProDKin_1	204	210	PF00069	0.405
MOD_ProDKin_1	323	329	PF00069	0.437
MOD_ProDKin_1	359	365	PF00069	0.683
MOD_ProDKin_1	47	53	PF00069	0.618
MOD_ProDKin_1	558	564	PF00069	0.665
MOD_ProDKin_1	56	62	PF00069	0.568
MOD_ProDKin_1	603	609	PF00069	0.577
MOD_ProDKin_1	764	770	PF00069	0.564
MOD_ProDKin_1	830	836	PF00069	0.431
MOD_ProDKin_1	882	888	PF00069	0.431
MOD_SUMO_rev_2	498	508	PF00179	0.326
MOD_SUMO_rev_2	760	764	PF00179	0.637
MOD_SUMO_rev_2	799	805	PF00179	0.382
TRG_DiLeu_BaLyEn_6	488	493	PF01217	0.527
TRG_ENDOCYTIC_2	341	344	PF00928	0.672
TRG_ER_diArg_1	1	4	PF00400	0.740
TRG_ER_diArg_1	262	264	PF00400	0.641
TRG_ER_diArg_1	300	302	PF00400	0.459
TRG_ER_diArg_1	337	340	PF00400	0.694
TRG_ER_diArg_1	408	411	PF00400	0.719
TRG_ER_diArg_1	46	48	PF00400	0.616
TRG_ER_diArg_1	573	576	PF00400	0.565
TRG_ER_diArg_1	696	698	PF00400	0.526
TRG_NLS_MonoCore_2	406	411	PF00514	0.593
TRG_NLS_MonoExtN_4	404	411	PF00514	0.801
TRG_Pf-PMV_PEXEL_1	586	590	PF00026	0.583
TRG_Pf-PMV_PEXEL_1	731	736	PF00026	0.469

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAU3	Leptomonas seymouri	48%	100%
A0A1X0NW19	Trypanosomatidae	33%	100%
A0A3R7LZC1	Trypanosoma rangeli	37%	100%
A4HKE6	Leishmania braziliensis	66%	100%
A4I7X4	Leishmania infantum	99%	100%
D0AA39	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
E9B2T5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%