SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

RNA polymerase-like protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X5F8_LEIDO

TriTrypDb:

LdBPK_321340.1 , LdCL_320019100 , LDHU3_32.1700

Length:

199

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0000932	P-body	5	1
GO:0005665	RNA polymerase II, core complex	4	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	1
GO:0099080	supramolecular complex	2	1
GO:0110165	cellular anatomical entity	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A0A3S7X5F8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X5F8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006352	DNA-templated transcription initiation	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009059	macromolecule biosynthetic process	4	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0032774	RNA biosynthetic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12
GO:0000291	nuclear-transcribed mRNA catabolic process, exonucleolytic	8	1
GO:0000956	nuclear-transcribed mRNA catabolic process	7	1
GO:0006367	transcription initiation at RNA polymerase II promoter	7	1
GO:0006401	RNA catabolic process	5	1
GO:0006402	mRNA catabolic process	6	1
GO:0006417	regulation of translation	6	1
GO:0006446	regulation of translational initiation	7	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0009891	positive regulation of biosynthetic process	5	1
GO:0009892	negative regulation of metabolic process	4	1
GO:0009893	positive regulation of metabolic process	4	1
GO:0009894	regulation of catabolic process	4	1
GO:0009896	positive regulation of catabolic process	5	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0010557	positive regulation of macromolecule biosynthetic process	6	1
GO:0010604	positive regulation of macromolecule metabolic process	5	1
GO:0010605	negative regulation of macromolecule metabolic process	5	1
GO:0010608	post-transcriptional regulation of gene expression	6	1
GO:0010628	positive regulation of gene expression	6	1
GO:0010629	negative regulation of gene expression	6	1
GO:0016071	mRNA metabolic process	6	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0019439	aromatic compound catabolic process	4	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031325	positive regulation of cellular metabolic process	5	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0031328	positive regulation of cellular biosynthetic process	6	1
GO:0031329	regulation of cellular catabolic process	5	1
GO:0031331	positive regulation of cellular catabolic process	6	1
GO:0034248	regulation of amide metabolic process	5	1
GO:0034250	positive regulation of amide metabolic process	6	1
GO:0034655	nucleobase-containing compound catabolic process	4	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0044270	cellular nitrogen compound catabolic process	4	1
GO:0045727	positive regulation of translation	7	1
GO:0045935	positive regulation of nucleobase-containing compound metabolic process	6	1
GO:0045948	positive regulation of translational initiation	8	1
GO:0046700	heterocycle catabolic process	4	1
GO:0048518	positive regulation of biological process	3	1
GO:0048519	negative regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051173	positive regulation of nitrogen compound metabolic process	5	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0051247	positive regulation of protein metabolic process	6	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0051254	positive regulation of RNA metabolic process	6	1
GO:0060211	regulation of nuclear-transcribed mRNA poly(A) tail shortening	7	1
GO:0060213	positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	8	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0061013	regulation of mRNA catabolic process	6	1
GO:0061014	positive regulation of mRNA catabolic process	7	1
GO:0065007	biological regulation	1	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901361	organic cyclic compound catabolic process	4	1
GO:1901575	organic substance catabolic process	3	1
GO:1903311	regulation of mRNA metabolic process	6	1
GO:1903313	positive regulation of mRNA metabolic process	7	1
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	1
GO:0003677	DNA binding	4	1
GO:0003697	single-stranded DNA binding	5	1
GO:0003723	RNA binding	4	1
GO:0003727	single-stranded RNA binding	5	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0031369	translation initiation factor binding	3	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1
GO:0003824	catalytic activity	1	1
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	1
GO:0016740	transferase activity	2	1
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	1
GO:0016779	nucleotidyltransferase activity	4	1
GO:0034062	5'-3' RNA polymerase activity	5	1
GO:0097747	RNA polymerase activity	4	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	192	198	PF00089	0.610
CLV_NRD_NRD_1	158	160	PF00675	0.647
CLV_PCSK_KEX2_1	158	160	PF00082	0.614
CLV_PCSK_SKI1_1	159	163	PF00082	0.543
CLV_PCSK_SKI1_1	24	28	PF00082	0.119
CLV_PCSK_SKI1_1	4	8	PF00082	0.555
DEG_APCC_DBOX_1	23	31	PF00400	0.536
DEG_Nend_Nbox_1	1	3	PF02207	0.536
DEG_SCF_FBW7_2	172	179	PF00400	0.558
DOC_ANK_TNKS_1	30	37	PF00023	0.450
DOC_CYCLIN_RxL_1	21	28	PF00134	0.536
DOC_MAPK_gen_1	158	169	PF00069	0.565
DOC_MAPK_gen_1	96	105	PF00069	0.530
DOC_MAPK_MEF2A_6	162	171	PF00069	0.572
DOC_MAPK_MEF2A_6	57	65	PF00069	0.536
DOC_MAPK_MEF2A_6	99	107	PF00069	0.526
DOC_PP4_FxxP_1	191	194	PF00568	0.617
DOC_USP7_MATH_1	151	155	PF00917	0.680
DOC_USP7_MATH_1	55	59	PF00917	0.514
DOC_WW_Pin1_4	172	177	PF00397	0.555
DOC_WW_Pin1_4	38	43	PF00397	0.542
LIG_14-3-3_CanoR_1	24	30	PF00244	0.522
LIG_14-3-3_CanoR_1	87	91	PF00244	0.643
LIG_Actin_WH2_2	15	33	PF00022	0.461
LIG_BRCT_BRCA1_1	82	86	PF00533	0.561
LIG_deltaCOP1_diTrp_1	142	145	PF00928	0.695
LIG_eIF4E_1	120	126	PF01652	0.648
LIG_FHA_1	8	14	PF00498	0.536
LIG_LIR_Gen_1	113	122	PF02991	0.638
LIG_LIR_Gen_1	89	97	PF02991	0.607
LIG_LIR_Nem_3	113	119	PF02991	0.527
LIG_MYND_1	190	194	PF01753	0.655
LIG_PCNA_yPIPBox_3	23	37	PF02747	0.519
LIG_Pex14_1	116	120	PF04695	0.649
LIG_RPA_C_Fungi	94	106	PF08784	0.635
LIG_SH2_CRK	3	7	PF00017	0.646
LIG_SH2_STAP1	90	94	PF00017	0.557
LIG_SH2_STAT5	52	55	PF00017	0.372
LIG_SUMO_SIM_anti_2	101	109	PF11976	0.518
LIG_SUMO_SIM_par_1	61	67	PF11976	0.291
LIG_WRC_WIRS_1	26	31	PF05994	0.423
MOD_CK1_1	141	147	PF00069	0.652
MOD_CK1_1	25	31	PF00069	0.423
MOD_CK1_1	41	47	PF00069	0.423
MOD_CK1_1	56	62	PF00069	0.202
MOD_GlcNHglycan	58	61	PF01048	0.342
MOD_GSK3_1	18	25	PF00069	0.396
MOD_GSK3_1	51	58	PF00069	0.399
MOD_GSK3_1	82	89	PF00069	0.632
MOD_N-GLC_1	22	27	PF02516	0.423
MOD_NEK2_1	115	120	PF00069	0.557
MOD_NEK2_1	18	23	PF00069	0.243
MOD_NEK2_1	7	12	PF00069	0.353
MOD_NEK2_2	82	87	PF00069	0.590
MOD_PKA_1	162	168	PF00069	0.675
MOD_PKA_2	157	163	PF00069	0.636
MOD_PKA_2	86	92	PF00069	0.636
MOD_Plk_1	141	147	PF00069	0.688
MOD_Plk_1	22	28	PF00069	0.369
MOD_Plk_4	115	121	PF00069	0.585
MOD_Plk_4	162	168	PF00069	0.675
MOD_Plk_4	25	31	PF00069	0.423
MOD_ProDKin_1	172	178	PF00069	0.554
MOD_ProDKin_1	38	44	PF00069	0.431
MOD_SUMO_rev_2	134	138	PF00179	0.695
TRG_DiLeu_BaEn_1	101	106	PF01217	0.540
TRG_ENDOCYTIC_2	3	6	PF00928	0.645
TRG_ENDOCYTIC_2	90	93	PF00928	0.523
TRG_ER_diArg_1	167	170	PF00400	0.559
TRG_Pf-PMV_PEXEL_1	170	174	PF00026	0.668

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEU0	Leptomonas seymouri	84%	100%
A0A0S4KJI5	Bodo saltans	38%	100%
A0A1X0NV48	Trypanosomatidae	50%	100%
A0A422P1E0	Trypanosoma rangeli	49%	100%
A4HKB6	Leishmania braziliensis	94%	100%
A4I7U5	Leishmania infantum	100%	100%
D0AA03	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
E9B2Q3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
O14459	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
P34087	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
P38421	Arabidopsis thaliana	27%	100%
P46279	Glycine max	27%	100%
Q4Q5F4	Leishmania major	97%	100%
Q54P04	Dictyostelium discoideum	28%	100%
V5BGQ6	Trypanosoma cruzi	47%	100%