LEM3_(Ligand-effect_modulator_3)_family_/_CDC50_f amily_putative/Pfam:PF03381

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

miltefosine transporter beta subunit

Species:

Leishmania donovani

UniProt:

A0A3S7X551_LEIDO

TriTrypDb:

LdBPK_320540.1 , LdCL_320010900 , LDHU3_32.0670

Length:

363

Annotations

LeishMANIAdb annotations

Homologous to animal CDC50 family phospholipid flippase (ATPase) proteins.. This family has expanded in kinetoplastids

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 16
NetGPI	no	yes: 0, no: 16

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	17
GO:0110165	cellular anatomical entity	1	17
GO:0005783	endoplasmic reticulum	5	1
GO:0005794	Golgi apparatus	5	1
GO:0005886	plasma membrane	3	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A0A3S7X551

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X551

Function

Biological processes
Term	Name	Level	Count
GO:0033036	macromolecule localization	2	17
GO:0051179	localization	1	17

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_SKI1_1	124	128	PF00082	0.509
CLV_PCSK_SKI1_1	207	211	PF00082	0.530
CLV_PCSK_SKI1_1	242	246	PF00082	0.417
CLV_PCSK_SKI1_1	97	101	PF00082	0.485
DOC_CYCLIN_yCln2_LP_2	106	112	PF00134	0.253
DOC_CYCLIN_yCln2_LP_2	145	151	PF00134	0.231
DOC_MAPK_FxFP_2	149	152	PF00069	0.291
DOC_MAPK_gen_1	284	293	PF00069	0.308
DOC_MAPK_MEF2A_6	25	33	PF00069	0.445
DOC_MAPK_MEF2A_6	284	293	PF00069	0.304
DOC_PP1_RVXF_1	56	62	PF00149	0.326
DOC_PP1_RVXF_1	95	101	PF00149	0.313
DOC_PP2B_LxvP_1	106	109	PF13499	0.280
DOC_PP2B_LxvP_1	145	148	PF13499	0.304
DOC_PP2B_LxvP_1	356	359	PF13499	0.476
DOC_PP4_FxxP_1	149	152	PF00568	0.270
DOC_USP7_MATH_1	214	218	PF00917	0.309
DOC_USP7_MATH_1	277	281	PF00917	0.327
DOC_USP7_MATH_1	310	314	PF00917	0.344
DOC_USP7_MATH_1	320	324	PF00917	0.325
DOC_USP7_UBL2_3	7	11	PF12436	0.668
DOC_WW_Pin1_4	114	119	PF00397	0.307
DOC_WW_Pin1_4	24	29	PF00397	0.563
LIG_14-3-3_CanoR_1	124	132	PF00244	0.315
LIG_14-3-3_CanoR_1	138	145	PF00244	0.265
LIG_14-3-3_CanoR_1	97	103	PF00244	0.296
LIG_APCC_ABBA_1	358	363	PF00400	0.512
LIG_BIR_II_1	1	5	PF00653	0.646
LIG_BRCT_BRCA1_1	116	120	PF00533	0.245
LIG_BRCT_BRCA1_1	31	35	PF00533	0.420
LIG_BRCT_BRCA1_1	312	316	PF00533	0.336
LIG_BRCT_BRCA1_1	67	71	PF00533	0.406
LIG_FHA_1	105	111	PF00498	0.287
LIG_FHA_1	125	131	PF00498	0.106
LIG_FHA_1	277	283	PF00498	0.287
LIG_FHA_1	340	346	PF00498	0.418
LIG_FHA_1	54	60	PF00498	0.394
LIG_FHA_2	55	61	PF00498	0.386
LIG_LIR_Apic_2	161	166	PF02991	0.325
LIG_LIR_Apic_2	221	226	PF02991	0.248
LIG_LIR_Gen_1	129	139	PF02991	0.317
LIG_LIR_Gen_1	313	320	PF02991	0.306
LIG_LIR_Gen_1	352	361	PF02991	0.463
LIG_LIR_Gen_1	60	71	PF02991	0.344
LIG_LIR_Nem_3	117	123	PF02991	0.306
LIG_LIR_Nem_3	129	135	PF02991	0.324
LIG_LIR_Nem_3	283	289	PF02991	0.251
LIG_LIR_Nem_3	297	302	PF02991	0.263
LIG_LIR_Nem_3	313	319	PF02991	0.213
LIG_LIR_Nem_3	32	38	PF02991	0.387
LIG_LIR_Nem_3	60	66	PF02991	0.307
LIG_MLH1_MIPbox_1	116	120	PF16413	0.190
LIG_NRBOX	331	337	PF00104	0.428
LIG_Pex14_2	79	83	PF04695	0.190
LIG_PTAP_UEV_1	306	311	PF05743	0.287
LIG_PTB_Apo_2	119	126	PF02174	0.342
LIG_PTB_Phospho_1	119	125	PF10480	0.287
LIG_SH2_CRK	132	136	PF00017	0.297
LIG_SH2_CRK	223	227	PF00017	0.315
LIG_SH2_CRK	286	290	PF00017	0.280
LIG_SH2_GRB2like	163	166	PF00017	0.342
LIG_SH2_NCK_1	63	67	PF00017	0.336
LIG_SH2_SRC	63	66	PF00017	0.308
LIG_SH2_STAT5	125	128	PF00017	0.297
LIG_SH2_STAT5	248	251	PF00017	0.302
LIG_SH2_STAT5	299	302	PF00017	0.247
LIG_SH2_STAT5	355	358	PF00017	0.547
LIG_SH3_3	2	8	PF00018	0.680
LIG_SH3_3	288	294	PF00018	0.319
LIG_SH3_3	304	310	PF00018	0.319
LIG_SH3_3	78	84	PF00018	0.336
LIG_SH3_4	7	14	PF00018	0.676
LIG_SH3_5	294	298	PF00018	0.319
LIG_SUMO_SIM_anti_2	30	35	PF11976	0.354
LIG_SUMO_SIM_anti_2	334	340	PF11976	0.447
LIG_SUMO_SIM_par_1	262	269	PF11976	0.190
LIG_SUMO_SIM_par_1	27	32	PF11976	0.540
LIG_SUMO_SIM_par_1	46	52	PF11976	0.158
LIG_TRFH_1	114	118	PF08558	0.420
LIG_TYR_ITIM	130	135	PF00017	0.448
LIG_WW_3	224	228	PF00397	0.339
MOD_CK1_1	133	139	PF00069	0.383
MOD_CK1_1	140	146	PF00069	0.389
MOD_CK1_1	193	199	PF00069	0.380
MOD_CK1_1	27	33	PF00069	0.437
MOD_CK1_1	280	286	PF00069	0.313
MOD_CK2_1	262	268	PF00069	0.339
MOD_GlcNHglycan	135	138	PF01048	0.338
MOD_GlcNHglycan	139	142	PF01048	0.318
MOD_GlcNHglycan	160	163	PF01048	0.445
MOD_GlcNHglycan	191	195	PF01048	0.369
MOD_GlcNHglycan	210	213	PF01048	0.385
MOD_GlcNHglycan	236	239	PF01048	0.394
MOD_GlcNHglycan	242	245	PF01048	0.365
MOD_GlcNHglycan	51	54	PF01048	0.490
MOD_GlcNHglycan	91	94	PF01048	0.415
MOD_GSK3_1	126	133	PF00069	0.307
MOD_GSK3_1	177	184	PF00069	0.325
MOD_GSK3_1	193	200	PF00069	0.366
MOD_GSK3_1	276	283	PF00069	0.411
MOD_GSK3_1	49	56	PF00069	0.481
MOD_GSK3_1	85	92	PF00069	0.329
MOD_GSK3_1	98	105	PF00069	0.299
MOD_N-GLC_1	175	180	PF02516	0.284
MOD_N-GLC_1	197	202	PF02516	0.373
MOD_N-GLC_1	240	245	PF02516	0.400
MOD_N-GLC_1	85	90	PF02516	0.305
MOD_N-GLC_2	206	208	PF02516	0.292
MOD_NEK2_1	175	180	PF00069	0.292
MOD_NEK2_1	29	34	PF00069	0.404
MOD_NEK2_1	339	344	PF00069	0.300
MOD_NEK2_1	346	351	PF00069	0.294
MOD_NEK2_1	85	90	PF00069	0.341
MOD_NEK2_1	98	103	PF00069	0.316
MOD_PIKK_1	130	136	PF00454	0.318
MOD_PKA_2	137	143	PF00069	0.366
MOD_PKA_2	214	220	PF00069	0.428
MOD_Plk_1	130	136	PF00069	0.376
MOD_Plk_1	175	181	PF00069	0.284
MOD_Plk_4	184	190	PF00069	0.386
MOD_Plk_4	29	35	PF00069	0.328
MOD_Plk_4	331	337	PF00069	0.381
MOD_Plk_4	346	352	PF00069	0.224
MOD_Plk_4	85	91	PF00069	0.430
MOD_ProDKin_1	114	120	PF00069	0.387
MOD_ProDKin_1	24	30	PF00069	0.462
TRG_ENDOCYTIC_2	123	126	PF00928	0.355
TRG_ENDOCYTIC_2	132	135	PF00928	0.370
TRG_ENDOCYTIC_2	286	289	PF00928	0.314
TRG_ENDOCYTIC_2	299	302	PF00928	0.273
TRG_ENDOCYTIC_2	355	358	PF00928	0.490
TRG_ENDOCYTIC_2	63	66	PF00928	0.365
TRG_Pf-PMV_PEXEL_1	287	292	PF00026	0.359

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBG2	Leptomonas seymouri	31%	87%
A0A0N1PDQ9	Leptomonas seymouri	60%	100%
A0A0S4IMK4	Bodo saltans	31%	90%
A0A0S4J4T1	Bodo saltans	40%	96%
A0A0S4JB98	Bodo saltans	31%	90%
A0A0S4JJY0	Bodo saltans	29%	90%
A0A0S4JQR9	Bodo saltans	33%	69%
A0A1X0NHQ5	Trypanosomatidae	31%	76%
A0A1X0NV71	Trypanosomatidae	40%	96%
A0A1X0P5L3	Trypanosomatidae	32%	91%
A0A3R7LIN7	Trypanosoma rangeli	31%	85%
A0A3R7MGX3	Trypanosoma rangeli	41%	97%
A0A3S7X9N4	Leishmania donovani	30%	86%
A0A422NR22	Trypanosoma rangeli	34%	91%
A4HK31	Leishmania braziliensis	73%	100%
A4HN07	Leishmania braziliensis	30%	100%
A4I7N1	Leishmania infantum	100%	100%
A4IBN0	Leishmania infantum	30%	100%
C9ZZ02	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	94%
D0A9K3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	77%
D0A9R9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AFG4	Leishmania major	31%	100%
E9B2H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
E9B6L9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
P25656	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	93%
P42838	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	88%
P53740	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	92%
Q17QL5	Bos taurus	31%	100%
Q1MTQ5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	92%
Q2T9P5	Bos taurus	25%	100%
Q3MIR4	Homo sapiens	28%	100%
Q4Q5N2	Leishmania major	91%	100%
Q5F362	Gallus gallus	31%	98%
Q5R6C0	Pongo abelii	30%	100%
Q67YS6	Arabidopsis thaliana	26%	100%
Q6AY41	Rattus norvegicus	30%	100%
Q8BHG3	Mus musculus	27%	100%
Q8L8W0	Arabidopsis thaliana	27%	100%
Q8VEK0	Mus musculus	31%	100%
Q95JK4	Macaca fascicularis	26%	100%
Q96WW4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	98%
Q9D4D7	Mus musculus	25%	100%
Q9LTW0	Arabidopsis thaliana	28%	100%
Q9NV96	Homo sapiens	30%	100%
Q9SA35	Arabidopsis thaliana	28%	100%
Q9SLK2	Arabidopsis thaliana	28%	100%
V5AY40	Trypanosoma cruzi	34%	100%
V5B6A6	Trypanosoma cruzi	42%	96%
V5BY23	Trypanosoma cruzi	31%	91%