Emp24/gp25L/p24 family/GOLD, putative

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

emp24/gp25L/p24 family/GOLD, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X4D1_LEIDO

TriTrypDb:

LdBPK_311120.1 , LdCL_310018000 , LDHU3_31.1850

Length:

325

Annotations

LeishMANIAdb annotations

Related to other eukaryotic COPII adaptors. Likely involved in ER to Golgi transport.. A Leishmaniid-specific subgroup of these proteins is missing the SigP (unclear topology and function). Localization: ER (by homology) / Golgi (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 4
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	7
GO:0110165	cellular anatomical entity	1	7
GO:0005783	endoplasmic reticulum	5	1
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	1
GO:0005794	Golgi apparatus	5	1
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	1
GO:0030135	coated vesicle	7	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1

Expansion

Sequence features

A0A3S7X4D1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X4D1

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006886	intracellular protein transport	4	1
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	1
GO:0006996	organelle organization	4	1
GO:0007030	Golgi organization	5	1
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0015031	protein transport	4	1
GO:0016043	cellular component organization	3	1
GO:0016192	vesicle-mediated transport	4	1
GO:0033036	macromolecule localization	2	1
GO:0045184	establishment of protein localization	3	1
GO:0046907	intracellular transport	3	1
GO:0048193	Golgi vesicle transport	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0071840	cellular component organization or biogenesis	2	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	119	121	PF00675	0.391
CLV_NRD_NRD_1	268	270	PF00675	0.410
CLV_NRD_NRD_1	3	5	PF00675	0.395
CLV_NRD_NRD_1	322	324	PF00675	0.424
CLV_NRD_NRD_1	56	58	PF00675	0.380
CLV_PCSK_KEX2_1	118	120	PF00082	0.403
CLV_PCSK_KEX2_1	3	5	PF00082	0.395
CLV_PCSK_KEX2_1	321	323	PF00082	0.451
CLV_PCSK_KEX2_1	56	58	PF00082	0.380
CLV_PCSK_PC1ET2_1	118	120	PF00082	0.363
CLV_PCSK_PC1ET2_1	321	323	PF00082	0.354
CLV_PCSK_SKI1_1	120	124	PF00082	0.353
CLV_PCSK_SKI1_1	56	60	PF00082	0.380
CLV_PCSK_SKI1_1	95	99	PF00082	0.396
DEG_APCC_DBOX_1	119	127	PF00400	0.530
DOC_CYCLIN_RxL_1	211	221	PF00134	0.255
DOC_CYCLIN_yCln2_LP_2	40	46	PF00134	0.567
DOC_MAPK_FxFP_2	179	182	PF00069	0.210
DOC_MAPK_gen_1	116	126	PF00069	0.548
DOC_MAPK_gen_1	56	63	PF00069	0.577
DOC_MAPK_MEF2A_6	119	128	PF00069	0.604
DOC_MAPK_MEF2A_6	27	36	PF00069	0.571
DOC_MAPK_MEF2A_6	56	63	PF00069	0.577
DOC_PP1_RVXF_1	212	219	PF00149	0.275
DOC_PP1_RVXF_1	290	296	PF00149	0.378
DOC_PP2B_LxvP_1	101	104	PF13499	0.583
DOC_PP2B_LxvP_1	205	208	PF13499	0.224
DOC_PP2B_LxvP_1	50	53	PF13499	0.574
DOC_PP4_FxxP_1	179	182	PF00568	0.210
DOC_PP4_FxxP_1	38	41	PF00568	0.568
DOC_USP7_MATH_1	228	232	PF00917	0.215
DOC_USP7_MATH_1	66	70	PF00917	0.569
DOC_WW_Pin1_4	197	202	PF00397	0.210
DOC_WW_Pin1_4	82	87	PF00397	0.608
LIG_14-3-3_CanoR_1	17	26	PF00244	0.559
LIG_14-3-3_CanoR_1	3	11	PF00244	0.585
LIG_14-3-3_CanoR_1	62	70	PF00244	0.570
LIG_14-3-3_CterR_2	322	325	PF00244	0.571
LIG_BRCT_BRCA1_1	230	234	PF00533	0.210
LIG_BRCT_BRCA1_1	34	38	PF00533	0.570
LIG_EH1_1	156	164	PF00400	0.210
LIG_FHA_1	158	164	PF00498	0.334
LIG_FHA_1	188	194	PF00498	0.229
LIG_FHA_1	211	217	PF00498	0.250
LIG_FHA_1	241	247	PF00498	0.210
LIG_FHA_2	258	264	PF00498	0.210
LIG_LIR_Apic_2	178	182	PF02991	0.430
LIG_LIR_Apic_2	35	41	PF02991	0.571
LIG_LIR_Gen_1	260	268	PF02991	0.210
LIG_LIR_Nem_3	35	40	PF02991	0.572
LIG_MLH1_MIPbox_1	34	38	PF16413	0.570
LIG_Pex14_1	94	98	PF04695	0.588
LIG_Pex14_2	38	42	PF04695	0.569
LIG_PTB_Apo_2	173	180	PF02174	0.210
LIG_SH2_CRK	74	78	PF00017	0.598
LIG_SH2_GRB2like	174	177	PF00017	0.210
LIG_SH2_GRB2like	204	207	PF00017	0.237
LIG_SH2_STAT5	204	207	PF00017	0.210
LIG_SH3_3	101	107	PF00018	0.580
LIG_SUMO_SIM_anti_2	125	130	PF11976	0.231
LIG_SUMO_SIM_par_1	122	127	PF11976	0.526
LIG_SUMO_SIM_par_1	225	231	PF11976	0.210
LIG_SUMO_SIM_par_1	30	35	PF11976	0.569
LIG_UBA3_1	261	270	PF00899	0.210
LIG_WRC_WIRS_1	301	306	PF05994	0.341
LIG_WRC_WIRS_1	315	320	PF05994	0.480
LIG_WRC_WIRS_1	33	38	PF05994	0.570
LIG_WRPW_2	94	97	PF00400	0.588
MOD_CK1_1	21	27	PF00069	0.560
MOD_CK1_1	230	236	PF00069	0.210
MOD_CK1_1	5	11	PF00069	0.581
MOD_CK1_1	65	71	PF00069	0.571
MOD_CK2_1	257	263	PF00069	0.210
MOD_CK2_1	314	320	PF00069	0.213
MOD_GlcNHglycan	144	147	PF01048	0.355
MOD_GlcNHglycan	21	24	PF01048	0.364
MOD_GlcNHglycan	50	53	PF01048	0.374
MOD_GlcNHglycan	64	67	PF01048	0.356
MOD_GSK3_1	106	113	PF00069	0.609
MOD_GSK3_1	151	158	PF00069	0.321
MOD_GSK3_1	206	213	PF00069	0.336
MOD_GSK3_1	62	69	PF00069	0.569
MOD_GSK3_1	73	80	PF00069	0.591
MOD_NEK2_1	124	129	PF00069	0.436
MOD_NEK2_1	136	141	PF00069	0.314
MOD_NEK2_1	220	225	PF00069	0.427
MOD_NEK2_1	227	232	PF00069	0.340
MOD_NEK2_1	314	319	PF00069	0.213
MOD_NEK2_1	32	37	PF00069	0.571
MOD_NEK2_2	66	71	PF00069	0.573
MOD_PIKK_1	220	226	PF00454	0.210
MOD_PKA_2	187	193	PF00069	0.380
MOD_PKA_2	2	8	PF00069	0.592
MOD_PKA_2	77	83	PF00069	0.600
MOD_PKB_1	60	68	PF00069	0.570
MOD_Plk_2-3	155	161	PF00069	0.281
MOD_Plk_4	124	130	PF00069	0.424
MOD_Plk_4	222	228	PF00069	0.380
MOD_Plk_4	257	263	PF00069	0.210
MOD_Plk_4	300	306	PF00069	0.256
MOD_Plk_4	32	38	PF00069	0.570
MOD_ProDKin_1	197	203	PF00069	0.210
MOD_ProDKin_1	82	88	PF00069	0.608
MOD_SUMO_rev_2	249	258	PF00179	0.372
TRG_DiLeu_BaLyEn_6	54	59	PF01217	0.575
TRG_ENDOCYTIC_2	204	207	PF00928	0.210
TRG_ENDOCYTIC_2	74	77	PF00928	0.596
TRG_ER_diArg_1	183	186	PF00400	0.252
TRG_ER_diArg_1	56	58	PF00400	0.580
TRG_ER_diArg_1	59	62	PF00400	0.574
TRG_NLS_MonoExtN_4	116	122	PF00514	0.562
TRG_Pf-PMV_PEXEL_1	214	219	PF00026	0.419
TRG_Pf-PMV_PEXEL_1	237	241	PF00026	0.412
TRG_Pf-PMV_PEXEL_1	269	273	PF00026	0.410
TRG_Pf-PMV_PEXEL_1	283	287	PF00026	0.403

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3S7X4G7	Leishmania donovani	41%	100%
A4HJ91	Leishmania braziliensis	41%	100%
A4I6N5	Leishmania infantum	41%	100%
E9B1Q8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	37%	100%
Q4Q6F2	Leishmania major	41%	100%